Post on 30-Dec-2015
description
7 13
PODperoxidase
11.1 H2O2+ AH22H2O+A
1.21 2
1.
20%POD2mol/L NaCl()569,000/g240/g
2 1 2 3
POD0.3%H2O2
3pH3.1pHpHpH5.0-5.4
3..35-60553540
44.1. POD
88 -
70 130 30-40%50 40
4.2.
4.3.
5 5.1. POD9510min12010min0.3%
5.2. POD40%
5.3. pHNaCl(pH 7.076)pH7pH 4.0pH1082NaCl(pH7.0NaCl0.6mo1L)
5.4. pHTTHTST
5.5.
6 6.1. POD
6.2. 2.1 SO2SO2H2O2 SO2+ H2O 2H2O+SO30.1-0.15%
6.2.2 6.2.3 6.2.4 pH5.5PODpHPODPODpH5.58.0pH
E1,10,3,1
O2
112PPO3PPOPPOPPOPPO
22.1. (1)
(2)
2.2.2.2.1 3,4 3,4
2.2.2PPO PPOCH2PPOPPO
3 pHPPOpH4-7PPOpHpHpHpHPPOpHpH
4 PPO337PPO22153570-90/
5O2
1PPOEDTA
2 SO2 SO2
3PVPP
4
6 01200 Lx()PPO()PPO
7
SDS () PVP()SDSSDS
Cu2+34
8 12PPO34
7 2
lipoxygenase, LOX EC 1.13.1.1320
1 7-114
14 CH3CH24CHCHCH2CHCH(CH2)7COOH CH3(CH2CH=CH)3(CH2)7COOH CH3(CH2)4(CH=CH-CH2)4(CH2)2COOH
148310(58111420)8111420
13L9D
2
3 pHpH7.08.0pH7.08.0pH7pH7-220pH
7-2 pH20(B)pH7.5pHpHpH920(A)pH7.0pH20
4
4.1
1%
(SH)SS
4.2
1
2AA
5 ()pH
80~10010pHpH3.88
E
GODGlucose OxidaseGOD-D- EC1.1.3.4-D-
1928MullerAspergillus nigerKusaiPazurSwobod-daP.Variable
1 1C6H12O6 + O2 C6H12O7 + H2O2
1C6H12O6 + 1/2O2 C6H12O71C6H12O6 + C2H5OH + O2 C6H12O7 + CH3CHO + H2O
1.1 -D-C1--160
7-14
2 pHpH5.6pH3.5pH6.5pH3.57.640pH8.02.0pH8.11090%pH4020%
pH30pH2.6pH3.27-15
7-15
3 3050-15840603080%GOD?
Q103060
4 Hg2+Ag+Cu2+ NH4OHFADD-
5 FAD2FAD160000
O2 Ping Pong Bi Bi
GL-D--D-
EFAD-D-EFADH2-D--D-D-H2O2H2OO2
6
6.1 0.5%0.6%
--------H2O2H2O2
6.2
1
21070
2040/L
3Vc1g/L20mg/L
477%20.6%
51020%
67-17
7-17
6
6.3
6.4
/5
Superoxide dismutaseSOD, EC1.15.1.1
300SODCu-Zn-SODMn-SODFe-SODCu-Zn-SOD32000
Mn-SOD4800030000Fe-SOD38000SODSODSOD
1O2+ O2+2HO2+H2 O2
SODO2H2O2H2O2H2O O2SODO2
2SODpH
2.155/15~3060/10~2565/10~157595
Cu-Zn-SODTm83960%51.2%SODMn-SODMn-SOD65~70
2.2 pHpHCu-Zn-SODpH3.695%ZnpH12.2SODpHpH5.39.5
2.3SODCu-Zn-SODMn-SODSODMn-SODSODMn-SOD
2.4Cu-Zn-SODCuZnZnCuCuMnFeCuMn-SODFe-SOD
2.5SODSDSEDTASODSDSEDTA6M8MSOD -SHSODCu-Zn-SODSOD
2.6SODSODSODMn-SODFe-SODMnFe
3
3.1 SOD100
SODO2
3.2 SOD O2SODSODXSODSOD
3.3 SOD SODSODSOD1988SODSOD
4 SOD
4.1 SODSODSOD37150min81%SODT37210min82%84%SODSOD
SODSODSOD
SODSOD
4.2 SOD
4.3 SODSOD
PPO3,4PPO3,460-7020-30GOD