7 13

PODperoxidase

11.1 H2O2+ AH22H2O+A

1.21 2

1.

20%POD2mol/L NaCl()569,000/g240/g

2 1 2 3

POD0.3%H2O2

3pH3.1pHpHpH5.0-5.4

3..35-60553540

44.1. POD

88 -

70 130 30-40%50 40

4.2.

4.3.

5 5.1. POD9510min12010min0.3%

5.2. POD40%

5.3. pHNaCl(pH 7.076)pH7pH 4.0pH1082NaCl(pH7.0NaCl0.6mo1L)

5.4. pHTTHTST

5.5.

6 6.1. POD

6.2. 2.1 SO2SO2H2O2 SO2+ H2O 2H2O+SO30.1-0.15%

6.2.2 6.2.3 6.2.4 pH5.5PODpHPODPODpH5.58.0pH

E1,10,3,1

O2

112PPO3PPOPPOPPOPPO

22.1. (1)

(2)

2.2.2.2.1 3,4 3,4

2.2.2PPO PPOCH2PPOPPO

3 pHPPOpH4-7PPOpHpHpHpHPPOpHpH

4 PPO337PPO22153570-90/

5O2

1PPOEDTA

2 SO2 SO2

3PVPP

4

6 01200 Lx()PPO()PPO

7

SDS () PVP()SDSSDS

Cu2+34

8 12PPO34

7 2

lipoxygenase, LOX EC 1.13.1.1320

1 7-114

14 CH3CH24CHCHCH2CHCH(CH2)7COOH CH3(CH2CH=CH)3(CH2)7COOH CH3(CH2)4(CH=CH-CH2)4(CH2)2COOH

148310(58111420)8111420

13L9D

2

3 pHpH7.08.0pH7.08.0pH7pH7-220pH

7-2 pH20(B)pH7.5pHpHpH920(A)pH7.0pH20

4

4.1

1%

(SH)SS

4.2

1

2AA

5 ()pH

80~10010pHpH3.88

E

GODGlucose OxidaseGOD-D- EC1.1.3.4-D-

1928MullerAspergillus nigerKusaiPazurSwobod-daP.Variable

1 1C6H12O6 + O2 C6H12O7 + H2O2

1C6H12O6 + 1/2O2 C6H12O71C6H12O6 + C2H5OH + O2 C6H12O7 + CH3CHO + H2O

1.1 -D-C1--160

7-14

2 pHpH5.6pH3.5pH6.5pH3.57.640pH8.02.0pH8.11090%pH4020%

pH30pH2.6pH3.27-15

7-15

3 3050-15840603080%GOD?

Q103060

4 Hg2+Ag+Cu2+ NH4OHFADD-

5 FAD2FAD160000

O2 Ping Pong Bi Bi

GL-D--D-

EFAD-D-EFADH2-D--D-D-H2O2H2OO2

6

6.1 0.5%0.6%

--------H2O2H2O2

6.2

1

21070

2040/L

3Vc1g/L20mg/L

477%20.6%

51020%

67-17

7-17

6

6.3

6.4

/5

Superoxide dismutaseSOD, EC1.15.1.1

300SODCu-Zn-SODMn-SODFe-SODCu-Zn-SOD32000

Mn-SOD4800030000Fe-SOD38000SODSODSOD

1O2+ O2+2HO2+H2 O2

SODO2H2O2H2O2H2O O2SODO2

2SODpH

2.155/15~3060/10~2565/10~157595

Cu-Zn-SODTm83960%51.2%SODMn-SODMn-SOD65~70

2.2 pHpHCu-Zn-SODpH3.695%ZnpH12.2SODpHpH5.39.5

2.3SODCu-Zn-SODMn-SODSODMn-SODSODMn-SOD

2.4Cu-Zn-SODCuZnZnCuCuMnFeCuMn-SODFe-SOD

2.5SODSDSEDTASODSDSEDTA6M8MSOD -SHSODCu-Zn-SODSOD

2.6SODSODSODMn-SODFe-SODMnFe

3

3.1 SOD100

SODO2

3.2 SOD O2SODSODXSODSOD

3.3 SOD SODSODSOD1988SODSOD

4 SOD

4.1 SODSODSOD37150min81%SODT37210min82%84%SODSOD

SODSODSOD

SODSOD

4.2 SOD

4.3 SODSOD

PPO3,4PPO3,460-7020-30GOD