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SCI期刊投稿策略與技巧

陳春榮台中榮民總醫院教學研究部

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學術研究成果呈現項目

學術期刊 專利 技術移轉 專書

如何撰寫高品質之期刊論文提升學術研究效率及論文寫作學術期刊投稿策略與技巧如何投稿SCI國外學術期刊期刊投稿經驗分享

“近年來投稿學術期刊已成為一個發表研究成果的重要管道”

希望藉由經驗的累積與分享，經驗傳承，熟悉研究方法與技巧，進而達到提昇成果發表的目的。

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正式論文，簡報型論文，病例報告，致編輯信，綜合評論等

學術期刊領域分類

Science Citation Index (SCI)

收錄科學技術類期刊

Engineering Index (EI)

Social Science Citation Index (SSCI)

Arts & Humanities Citation Index (A&HCI)

非SCI

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Impact factor

The frequency of “average article” in a Journal has been

cited in a year.

Dividing the number of citations to articles published in

the two previous years by the total number of articles

Published in the two previous years.

A Journal’s relative importance within the same field.

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學術研究成果努力追求目標

以SCI論文而言

質 量

高Impact Factor

高Citation篇數多

第一/通訊作者共同作者

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SCI學術研究成果衡量影響面

學校或研究機構聲譽研究學者績效評量研究計畫申請執行能力評量學術課程工作機會職務升遷薪資等

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國科會生物處研究成果統計及獲獎情形

國科會AB表及RPI分數

表A

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表B

研究表現指數RPI(Research Performance Index)

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評比論文篇數

分母

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C x J x A

分子

SCI論文

C

J

A

11

12

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學術期刊選擇

Impact factor

領域排名同領域參考主流發行量/期數接受率/退稿率刊出時間費用投稿方式

Aims and Scope

Format

Relevant topics

Criteria

Matched Journal

Special Issue

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Journal homepage

Content

Author instruction

Journal homepage

Content

Author instruction

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資料庫

Year

Category

Specific Journal

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17

36/211=17.06%

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SCI學術期刊論文發表流程

1. In preparation:

2. Submission: Submitted

Acknowledgement letter

Rejected without reviewing

Format requirement check

Reviewing process

Reviewing comment

Rejected

Revision requested

Accepted

Manuscript

In preparation

In submission

Rejection

In revision

Acceptance

Proof

In press

Copyright transfer

Reprint

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SCI學術期刊論文發表流程

3. Resubmission: Revised

Acknowledgement letter

Reviewing comment

Rejected

Revision requested

Accepted

4. Proofreading: In press

Proof

Charge

Copyright transfer

5. Published: Reprint

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On-line submission

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Article type

Classification

Title

Authors

Abstract

Keywords

Comments

Upload files

Cover Letter

Reviewer suggestion

Manuscript

Figures

Tables

Author agreement

Conflict statement

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PDF

View check

Ethic comments

Approval

Manuscript number

Primary reviewing ~2 Months

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同儕審查

Reviewer suggestion

學術期刊論文審查流程

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29

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Ref.: Ms. No. LFS-D-09-00849

Anti-inflammatory characterization of quercetin in activated microglia

Life Sciences

Dear Professor Chen,

First let us apologize for the long handling time.

Your manuscript has been examined by the editors and two qualified reviewer. We are pleased to inform you that they find the

manuscript worthy of publication if revised in accordance with the enclosed comments.

Please incorporate responses to the reviewer comments into the revised paper. A complete rebuttal with no manuscript alterations is

usually considered inadequate.

A letter detailing your revisions point-by-point must accompany the resubmission. You will be requested to upload this Response to

Reviewers as a separate file in the Attach Files area.

To facilitate the assessment of your revised paper, please also provide an extra copy of the manuscript file where all changes have been

marked by using highlighting or color text. Please do not use the "track changes" function. Apart from the markings, this file should

be exactly the same as the manuscript file. This "marked version" should be uploaded in the Attach Files area. Please check the final

version of the pdf file carefully to ensure that the marked manuscript is included in the resubmission.

We ask that you resubmit your manuscript within 45 days. After this time, your file will be placed on inactive status and a further

submission will be considered a new manuscript.

To submit a revision, go to http://ees.elsevier.com/lfs/ and log in as an Author. You will see a menu item called Submission Needing

Revision. You will find your submission record there.

Summary of items required in your resubmission:

1) Point-by-point responses to editor and reviewer comments

2) Revised manuscript

3) All figures and tables

4) Marked copy of revised manuscript

Yours sincerely,

Janet Holmén

Managing Editor

Life Sciences

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COMMENTS FROM THE SCIENTIFIC EDITORS:

The manuscript is interesting but, in addition to the comments by the reviewers, attention should be paid to the following points:

1. Change the title to better describe the main findings of the study.

2. When presenting effects of quercetin on various factors (e.g. percentage inhibition of something), give the concentration of quercetin

which caused the effect in question; this should be checked throughout the Results section.

3. Results, page 9, when you measure phosphorylation of signaling molecules (e.g. ERK, JNK, P38...) as a marker of their activation,

change the wording accordingly (i.e. change activity / activation / inactivation to phospohorylation / reduced phosphorylation) to

describe the phenomenon which was actually measured.

4. Explain what are lipid drafts.

FORMAT SUGGESTIONS:

(1) Access the Guide to Authors at our website to check the format of your article. NOTE that the Results and the Discussion must be

presented in different sections.

(2) The language in your paper is generally clear, but sometimes uses far more words than necessary. This type of inefficient writing is

particularly obvious in the Abstract. Please get help from an experienced colleague or a professional language editor to improve and

streamline your presentation. A list of scientific language editing companies can be found on our website at

http://www.elsevier.com/wps/find/authorsview.authors/languagepolishing

(3) The reference format in your article is not quite correct. Please do not use initials in references in text. If you need to distinguish

between articles by Chen CC and Chen CJ, this should be done by giving publication year and - if necessary - letters, e.g. 2004a, 2004b,

2004c.

(4) We encourage you to test the relevance of your key words by using them for a database search and comparing the results with the

topic of your own paper.

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REVIEWERS' COMMENTS:

Reviewer #1: In this manuscript, authors characterize quercetin's anti-inflammatory profile in activated

BV-2 microglia. Quercetin was found to down-regulate activation of p38, ERK, Akt, Scr, JAK-2, Tyk2,

STAT-1 and NF-<kappa>B along with its inhibitory effect on NO production and iNOS expression in cells

treated with LPS and IFN<gamma>. In addition, quercetin was found to suppress free radical generation in

LPS/IFN<gamma> treated cells as compared to cells treated with stimuli only. The accumulation of lipid

rafts was disrupted by quercetin.

Few minor points:

1. In Materials and Methods; Cell culture: Please check how the origin of the cells was written in Chen CJ

et al. 2004 (cell line was kindly donated by..). In my opinion, it should be stated here in similar manner, and

the reference is then unnecessary.

2. In Materials and Methods; NO determination: Chen CJ et al. 2004 is not the correct reference for the

Griess reaction. Please correct this. In addition, Griess reaction measures nitrite, not nitrate (unless

reduction step is included), please correct this in Materials and Methods, page 5.

3. In Materials and Methods; Isolation of RNA..: Please check also here the use of Chen CJ et al. 2004 as a

reference - is the method reported where indicated?

4. In Materials and Methods; Preparation of nuclear..: Please consider if you could omit some of the text

when you refer to Chen CJ et al. 2004

5. Figure legends, Figure 5. (B) at the end of the text should propably not be there.

Reviewer #2: The submitted manuscript describes the modulatory effect of quercetin against

neuroinflammation. Materials and Methods section is complete and methods are well described.

Experiments are clear cut. Results are interesting above all from a speculative point of view. Results are

adequately discussed in the light of most recent papers on this matter.

Brief description

Weakness

Strength

Specific comments

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Journal of Virology 5.308

Biochemical Pharmacology 4.838

Journal of Neurochemistry 4.500

Journal of Nutritional Biochemistry 4.352

Diabetes Obesity and Metabolism 4.259

Experimental Neurology 3.974

Neurosurgery 3.398

European Journal of Neuroscience 3.385

Neurochemistry International 3.228

Journal of General Virology 3.092

European Journal of Pharmacology 2.787

Biochemical and Biophysical Research Communications 2.648

Life Science 2.583

NeuroToxicology 2.409

Neurochemical Research 2.260

Neuroscience Letters 2.200

NeuroReport 1.904

American Journal of Chinese Medicine 1.058

Journal IF

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Journal ReviewerNeuroscience Letters

Journal of Neurochemistry

Brain Research

Glia

Journal of Neuroscience Research

Acta Pharmacologica Sinica

European Journal of Neuroscience

Experimental Neurology

Transactions of the Royal Society of Tropical Medicine and Hygiene

PHARMACOLOGICAL REPORTS

Neurochemical Research

Chemical Research in Toxicology

Neurochemistry International

BMC Neurology

Brain Research Bulletin

Cell Biology International

Neuroscience

Journal of Medical Virology

Planta Medica

Life Sciences

NeuroToxicology

Pharmaceutical Biology

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學術期刊論文撰寫流程及注意事項

Title page

Abstract and key words

Introduction

Materials and Methods

Results

Discussion

Conclusion

Acknowledgment

Reference

Figure legend

Tables

Figures

整理實驗結果、數據繪圖蒐集研討相關文獻擬定論文訴求主軸重點依結果選定適切期刊依期刊格式列出大綱撰寫稿件擬定題目及作者論文稿件修改撰寫cover letter

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實驗數據的完整性新穎性技術創新觀念想法創新實驗設計邏輯實驗結果闡述、論文撰寫

成果內容部份

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Neuroprotective effect of atorvastatin in an experimental model of nerve crush injury

Statins have therapeutic benefit for the management of several disorders. A short-term course

of high-dose statins pre-treatment seems to have a neuroprotective effect. However, the

molecular basis underlying their neuroprotective action remains unclear. We investigated

whether a short-term course of high-dose atorvastatin pre-treatment has neuroprotective effects

on biochemical, functional, electrophysiological, and morphological alterations occurring

during injury-induced degeneration/regeneration through a rat model of sciatic nerve crush

injury. Atorvastatin or saline was given orally to rats for 7 days before injury. Motor function

recovery, compound muscle action potential, nerve conduction latency, and axonal integrity

showed significant improvement in the atorvastatin-treated group. Crush injury disrupted

nerve integrity, produced oxidative stress, induced inflammatory changes, and caused cell

apoptosis, and these detrimental alterations were attenuated by atorvastatin. After injury,

parameters of restorative potential were further upregulated in groups receiving atorvastatin,

including cell proliferation and viability and elevated expression of neurofilament, growth-

associated protein-43, myelin basic protein, ciliary neurotrophic factor, and collagen. The

progression of degeneration/regeneration after crush injury was accompanied by elevated

activity of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), Akt,

signal transducer and activator transcription-1 (STAT1), Smad2/3, NF-B, and AP-1.

Intriguingly, the suppression of ERK, AKT, STAT1, and NF-B and the elevated activation of

JNK, Smad2/3, and AP-1 were both associated with the neuroprotective action of atorvastatin.

These findings suggest that a short-term course of high-dose atorvastatin pre-treatment can

protect against sciatic nerve crush injury through directly altering primary insult and/or

modifying intracellular or extracellular environment making it favorable for regeneration.

實驗條件與臨床的差異、原因、相關實驗的差異、原因

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Conclusion

In summary, our findings indicate that pre-administration of atorvastatin led to

improved functional, biochemical, electrophysiological, and morphological outcomes

in a rat model of sciatic nerve crush injury. Although we did not fully establish a

direct causal relationship between signaling molecules, transcription factors, and

gene expression profiles, this improvement was associated with attenuated activity of

cytotoxic molecules and stimulated activity of cytoprotective molecules, suggesting

that the neuroprotective effect of atorvastatin following sciatic nerve crush injury

may be attributable, in part, to a dampening of damaging potential and

strengthening of regenerating potential. That is, rather than directly altering

primary insult, atorvastatin might modify intracellular or extracellular environment

making it favorable for regeneration after peripheral nerve crush injury. The

findings imply that hypercholesterolemia patients receiving statin treatment might

suffer less severity from peripheral nerve injury. However, the clinical relevance

(such as dosage and treatment course) for therapeutic intervention against

peripheral nerve injury by atorvastatin warrants further investigation.

過度闡訴結論

45訴求重點、機制、差異、結論

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主軸標的分子介紹實驗設計邏輯檢體分析標的

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Graptopetalum paraguayense E. Walther, a traditional Chinese herbal medicine, possesses

several biological/pharmacological activities including hepatoprotective, anti-oxidant, and anti-

inflammatory. The aim of this study was to evaluate the effect of Graptopetalum paraguayense E.

Walther extracts on inflammation-associated brain injury and neuroinflammation. Water

(GWE), 50% alcohol (GE50) extracts of Graptopetalum paraguayense E. Walther, and extracts

obtained from further extraction of GE50 with ethyl acetate (GEE) were used in this study.

Focal cerebral ischemia/reperfusion injured rat was used as a model to evaluate the

neuroprotective and anti-inflammatory effects of Graptopetalum paraguayense E. Walther

extracts. Its anti-inflammatory mechanism was further investigated on lipopolysaccharide

(LPS)/interferon- (IFN-)-activated BV-2 microglial cells. Oral administration of GEE, but not

GWE or GE50, for 2 weeks protected animals against ischemic brain injury The

neuroprotective effect of GEE was accompanied by decreased caspase-3 activity,

malondialdehyde content, and inducible nitric oxide synthase (iNOS) expression. GEE

decreased H2O2- and LPS/IFN--induced free radical generation and LPS/IFN--induced iNOS

expression in BV-2 microglial cells. Mechanistic study revealed that the neuroactive effects of

GEE were markedly associated with anti-oxidative potential, activation of serine/threonine and

tyrosine phosphatase, and down-regulation of extracellular signal-regulated kinase (ERK), c-

Jun N-terminal kinase (JNK), p38, Akt, Src, Janus kinase-1 (Jak1), Tyk2, signal transducer and

activator of transcription-1 (STAT1), and NF-B. Furthermore, the neuroprotective and anti-

inflammatory effects of Graptopetalum paraguayense E. Walther extracts were positively

paralleled to their polyphenolic contents such as gallic acid, genistin, daidzin, and quercetin.

These results suggest that Graptopetalum paraguayense E. Walther extracts protected against

ischemic brain injury and the neuroprotective effect might be associated with their ability to

scavenge free radicals and attenuate NO production in microglia.

中草藥典籍應用、適應症、使用方式、有效成份

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The nonregenerative capability of the injured adult brain has been challenged in

recent years and neural plasticity has been observed experimentally in both global

and focal brain ischemia in animal models. Whether neuro-genesis increases in

response to brain lesios or stem cells can be used for transplantation are the

potential questions to be answered. Here, we performed the middle cerebral artery

occlusion (MCAO) operation at rodent model and try to rescue neurons

surrounding insult from the ischemic damage using acute transplantation of neural

stem cells (NSCs). The infarct volumes and brain atrophy were obvious diminished

at grafted NSCs treatment. The behavior recovery of grafted NSCs treatment

significantly improved compared with vehicle control. Furthermore, we detected

the inflammation related moleculars such as COX-2 and IL-1β and found that the

grfated NSCs treatment after ischemic stroke could repressed the expression of

inflammation moleculars. We also detected the protein level of heat shock protein

27 (HSP27) as a protective protein against apoptosis. The results showed that the

group of grafted NSCs treatment induced the protein level of HSP27 and down-

regulated activity of caspase-3 compared with vehicle control. Our results

demonstrate that transplanted NSCs provide benefit in behavioral function

recovery after MCAO in rats and more neuroprotection or less inflammatory

destruction. These data reveal the another essential explain of cellular

transplantation therapy in damage recovery from ischemic stroke and offer new

therapeutic possibilities.

訴求邏輯、結果闡訴、結論、英文

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The goal of this study was to screen plants for anti-allergic effects by evaluating the

amounts of histamine and leukotrienes released after guinea pig lung mast cell

activation. Bioassay-guided fractionation led to two flavonoids, baicalin from

Scutellaria baicalenesis and isoorientin from Phyllastachys edulis. Based on these

compounds, we developed two standardized extracts, SSBE from S. baicalensis and

SPEE from P. edulis. SSBE or SPEE remarkably inhibited histamine and leukotrienes

release in a dose-dependent manner. We also prepared Uniflavon™ by combining these

extracts at a 1:2 ratio; the resulting combination had a stronger activity than either

extract alone. These data suggest that Uniflavon™ in low dose than in dose of each

standardized extract alone may be utilized as a therapeutic agent not only for allergic

diseases but also for immune diseases related to mast cells, including rheumatoid

arthritis and osteoarthritis, in further studies.

Anti-allergic Flavonoids, Baicalin and Isoorientin, from Scutellaria

baicalensis and Phyllostachys edulis

論訴重點一致性

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