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學術研究成果呈現項目
學術期刊 專利 技術移轉 專書
如何撰寫高品質之期刊論文提升學術研究效率及論文寫作學術期刊投稿策略與技巧如何投稿SCI國外學術期刊期刊投稿經驗分享
“近年來投稿學術期刊已成為一個發表研究成果的重要管道”
希望藉由經驗的累積與分享,經驗傳承,熟悉研究方法與技巧,進而達到提昇成果發表的目的。
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正式論文,簡報型論文,病例報告,致編輯信,綜合評論等
學術期刊領域分類
Science Citation Index (SCI)
收錄科學技術類期刊
Engineering Index (EI)
Social Science Citation Index (SSCI)
Arts & Humanities Citation Index (A&HCI)
非SCI
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Impact factor
The frequency of “average article” in a Journal has been
cited in a year.
Dividing the number of citations to articles published in
the two previous years by the total number of articles
Published in the two previous years.
A Journal’s relative importance within the same field.
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學術期刊選擇
Impact factor
領域排名同領域參考主流發行量/期數接受率/退稿率刊出時間費用投稿方式
Aims and Scope
Format
Relevant topics
Criteria
Matched Journal
Special Issue
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SCI學術期刊論文發表流程
1. In preparation:
2. Submission: Submitted
Acknowledgement letter
Rejected without reviewing
Format requirement check
Reviewing process
Reviewing comment
Rejected
Revision requested
Accepted
Manuscript
In preparation
In submission
Rejection
In revision
Acceptance
Proof
In press
Copyright transfer
Reprint
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SCI學術期刊論文發表流程
3. Resubmission: Revised
Acknowledgement letter
Reviewing comment
Rejected
Revision requested
Accepted
4. Proofreading: In press
Proof
Charge
Copyright transfer
5. Published: Reprint
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Article type
Classification
Title
Authors
Abstract
Keywords
Comments
Upload files
Cover Letter
Reviewer suggestion
Manuscript
Figures
Tables
Author agreement
Conflict statement
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Ref.: Ms. No. LFS-D-09-00849
Anti-inflammatory characterization of quercetin in activated microglia
Life Sciences
Dear Professor Chen,
First let us apologize for the long handling time.
Your manuscript has been examined by the editors and two qualified reviewer. We are pleased to inform you that they find the
manuscript worthy of publication if revised in accordance with the enclosed comments.
Please incorporate responses to the reviewer comments into the revised paper. A complete rebuttal with no manuscript alterations is
usually considered inadequate.
A letter detailing your revisions point-by-point must accompany the resubmission. You will be requested to upload this Response to
Reviewers as a separate file in the Attach Files area.
To facilitate the assessment of your revised paper, please also provide an extra copy of the manuscript file where all changes have been
marked by using highlighting or color text. Please do not use the "track changes" function. Apart from the markings, this file should
be exactly the same as the manuscript file. This "marked version" should be uploaded in the Attach Files area. Please check the final
version of the pdf file carefully to ensure that the marked manuscript is included in the resubmission.
We ask that you resubmit your manuscript within 45 days. After this time, your file will be placed on inactive status and a further
submission will be considered a new manuscript.
To submit a revision, go to http://ees.elsevier.com/lfs/ and log in as an Author. You will see a menu item called Submission Needing
Revision. You will find your submission record there.
Summary of items required in your resubmission:
1) Point-by-point responses to editor and reviewer comments
2) Revised manuscript
3) All figures and tables
4) Marked copy of revised manuscript
Yours sincerely,
Janet Holmén
Managing Editor
Life Sciences
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COMMENTS FROM THE SCIENTIFIC EDITORS:
The manuscript is interesting but, in addition to the comments by the reviewers, attention should be paid to the following points:
1. Change the title to better describe the main findings of the study.
2. When presenting effects of quercetin on various factors (e.g. percentage inhibition of something), give the concentration of quercetin
which caused the effect in question; this should be checked throughout the Results section.
3. Results, page 9, when you measure phosphorylation of signaling molecules (e.g. ERK, JNK, P38...) as a marker of their activation,
change the wording accordingly (i.e. change activity / activation / inactivation to phospohorylation / reduced phosphorylation) to
describe the phenomenon which was actually measured.
4. Explain what are lipid drafts.
FORMAT SUGGESTIONS:
(1) Access the Guide to Authors at our website to check the format of your article. NOTE that the Results and the Discussion must be
presented in different sections.
(2) The language in your paper is generally clear, but sometimes uses far more words than necessary. This type of inefficient writing is
particularly obvious in the Abstract. Please get help from an experienced colleague or a professional language editor to improve and
streamline your presentation. A list of scientific language editing companies can be found on our website at
http://www.elsevier.com/wps/find/authorsview.authors/languagepolishing
(3) The reference format in your article is not quite correct. Please do not use initials in references in text. If you need to distinguish
between articles by Chen CC and Chen CJ, this should be done by giving publication year and - if necessary - letters, e.g. 2004a, 2004b,
2004c.
(4) We encourage you to test the relevance of your key words by using them for a database search and comparing the results with the
topic of your own paper.
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REVIEWERS' COMMENTS:
Reviewer #1: In this manuscript, authors characterize quercetin's anti-inflammatory profile in activated
BV-2 microglia. Quercetin was found to down-regulate activation of p38, ERK, Akt, Scr, JAK-2, Tyk2,
STAT-1 and NF-<kappa>B along with its inhibitory effect on NO production and iNOS expression in cells
treated with LPS and IFN<gamma>. In addition, quercetin was found to suppress free radical generation in
LPS/IFN<gamma> treated cells as compared to cells treated with stimuli only. The accumulation of lipid
rafts was disrupted by quercetin.
Few minor points:
1. In Materials and Methods; Cell culture: Please check how the origin of the cells was written in Chen CJ
et al. 2004 (cell line was kindly donated by..). In my opinion, it should be stated here in similar manner, and
the reference is then unnecessary.
2. In Materials and Methods; NO determination: Chen CJ et al. 2004 is not the correct reference for the
Griess reaction. Please correct this. In addition, Griess reaction measures nitrite, not nitrate (unless
reduction step is included), please correct this in Materials and Methods, page 5.
3. In Materials and Methods; Isolation of RNA..: Please check also here the use of Chen CJ et al. 2004 as a
reference - is the method reported where indicated?
4. In Materials and Methods; Preparation of nuclear..: Please consider if you could omit some of the text
when you refer to Chen CJ et al. 2004
5. Figure legends, Figure 5. (B) at the end of the text should propably not be there.
Reviewer #2: The submitted manuscript describes the modulatory effect of quercetin against
neuroinflammation. Materials and Methods section is complete and methods are well described.
Experiments are clear cut. Results are interesting above all from a speculative point of view. Results are
adequately discussed in the light of most recent papers on this matter.
Brief description
Weakness
Strength
Specific comments
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Journal of Virology 5.308
Biochemical Pharmacology 4.838
Journal of Neurochemistry 4.500
Journal of Nutritional Biochemistry 4.352
Diabetes Obesity and Metabolism 4.259
Experimental Neurology 3.974
Neurosurgery 3.398
European Journal of Neuroscience 3.385
Neurochemistry International 3.228
Journal of General Virology 3.092
European Journal of Pharmacology 2.787
Biochemical and Biophysical Research Communications 2.648
Life Science 2.583
NeuroToxicology 2.409
Neurochemical Research 2.260
Neuroscience Letters 2.200
NeuroReport 1.904
American Journal of Chinese Medicine 1.058
Journal IF
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Journal ReviewerNeuroscience Letters
Journal of Neurochemistry
Brain Research
Glia
Journal of Neuroscience Research
Acta Pharmacologica Sinica
European Journal of Neuroscience
Experimental Neurology
Transactions of the Royal Society of Tropical Medicine and Hygiene
PHARMACOLOGICAL REPORTS
Neurochemical Research
Chemical Research in Toxicology
Neurochemistry International
BMC Neurology
Brain Research Bulletin
Cell Biology International
Neuroscience
Journal of Medical Virology
Planta Medica
Life Sciences
NeuroToxicology
Pharmaceutical Biology
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學術期刊論文撰寫流程及注意事項
Title page
Abstract and key words
Introduction
Materials and Methods
Results
Discussion
Conclusion
Acknowledgment
Reference
Figure legend
Tables
Figures
整理實驗結果、數據繪圖蒐集研討相關文獻擬定論文訴求主軸重點依結果選定適切期刊依期刊格式列出大綱撰寫稿件擬定題目及作者論文稿件修改撰寫cover letter
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Neuroprotective effect of atorvastatin in an experimental model of nerve crush injury
Statins have therapeutic benefit for the management of several disorders. A short-term course
of high-dose statins pre-treatment seems to have a neuroprotective effect. However, the
molecular basis underlying their neuroprotective action remains unclear. We investigated
whether a short-term course of high-dose atorvastatin pre-treatment has neuroprotective effects
on biochemical, functional, electrophysiological, and morphological alterations occurring
during injury-induced degeneration/regeneration through a rat model of sciatic nerve crush
injury. Atorvastatin or saline was given orally to rats for 7 days before injury. Motor function
recovery, compound muscle action potential, nerve conduction latency, and axonal integrity
showed significant improvement in the atorvastatin-treated group. Crush injury disrupted
nerve integrity, produced oxidative stress, induced inflammatory changes, and caused cell
apoptosis, and these detrimental alterations were attenuated by atorvastatin. After injury,
parameters of restorative potential were further upregulated in groups receiving atorvastatin,
including cell proliferation and viability and elevated expression of neurofilament, growth-
associated protein-43, myelin basic protein, ciliary neurotrophic factor, and collagen. The
progression of degeneration/regeneration after crush injury was accompanied by elevated
activity of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), Akt,
signal transducer and activator transcription-1 (STAT1), Smad2/3, NF-B, and AP-1.
Intriguingly, the suppression of ERK, AKT, STAT1, and NF-B and the elevated activation of
JNK, Smad2/3, and AP-1 were both associated with the neuroprotective action of atorvastatin.
These findings suggest that a short-term course of high-dose atorvastatin pre-treatment can
protect against sciatic nerve crush injury through directly altering primary insult and/or
modifying intracellular or extracellular environment making it favorable for regeneration.
實驗條件與臨床的差異、原因、相關實驗的差異、原因
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Conclusion
In summary, our findings indicate that pre-administration of atorvastatin led to
improved functional, biochemical, electrophysiological, and morphological outcomes
in a rat model of sciatic nerve crush injury. Although we did not fully establish a
direct causal relationship between signaling molecules, transcription factors, and
gene expression profiles, this improvement was associated with attenuated activity of
cytotoxic molecules and stimulated activity of cytoprotective molecules, suggesting
that the neuroprotective effect of atorvastatin following sciatic nerve crush injury
may be attributable, in part, to a dampening of damaging potential and
strengthening of regenerating potential. That is, rather than directly altering
primary insult, atorvastatin might modify intracellular or extracellular environment
making it favorable for regeneration after peripheral nerve crush injury. The
findings imply that hypercholesterolemia patients receiving statin treatment might
suffer less severity from peripheral nerve injury. However, the clinical relevance
(such as dosage and treatment course) for therapeutic intervention against
peripheral nerve injury by atorvastatin warrants further investigation.
過度闡訴結論
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Graptopetalum paraguayense E. Walther, a traditional Chinese herbal medicine, possesses
several biological/pharmacological activities including hepatoprotective, anti-oxidant, and anti-
inflammatory. The aim of this study was to evaluate the effect of Graptopetalum paraguayense E.
Walther extracts on inflammation-associated brain injury and neuroinflammation. Water
(GWE), 50% alcohol (GE50) extracts of Graptopetalum paraguayense E. Walther, and extracts
obtained from further extraction of GE50 with ethyl acetate (GEE) were used in this study.
Focal cerebral ischemia/reperfusion injured rat was used as a model to evaluate the
neuroprotective and anti-inflammatory effects of Graptopetalum paraguayense E. Walther
extracts. Its anti-inflammatory mechanism was further investigated on lipopolysaccharide
(LPS)/interferon- (IFN-)-activated BV-2 microglial cells. Oral administration of GEE, but not
GWE or GE50, for 2 weeks protected animals against ischemic brain injury The
neuroprotective effect of GEE was accompanied by decreased caspase-3 activity,
malondialdehyde content, and inducible nitric oxide synthase (iNOS) expression. GEE
decreased H2O2- and LPS/IFN--induced free radical generation and LPS/IFN--induced iNOS
expression in BV-2 microglial cells. Mechanistic study revealed that the neuroactive effects of
GEE were markedly associated with anti-oxidative potential, activation of serine/threonine and
tyrosine phosphatase, and down-regulation of extracellular signal-regulated kinase (ERK), c-
Jun N-terminal kinase (JNK), p38, Akt, Src, Janus kinase-1 (Jak1), Tyk2, signal transducer and
activator of transcription-1 (STAT1), and NF-B. Furthermore, the neuroprotective and anti-
inflammatory effects of Graptopetalum paraguayense E. Walther extracts were positively
paralleled to their polyphenolic contents such as gallic acid, genistin, daidzin, and quercetin.
These results suggest that Graptopetalum paraguayense E. Walther extracts protected against
ischemic brain injury and the neuroprotective effect might be associated with their ability to
scavenge free radicals and attenuate NO production in microglia.
中草藥典籍應用、適應症、使用方式、有效成份
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The nonregenerative capability of the injured adult brain has been challenged in
recent years and neural plasticity has been observed experimentally in both global
and focal brain ischemia in animal models. Whether neuro-genesis increases in
response to brain lesios or stem cells can be used for transplantation are the
potential questions to be answered. Here, we performed the middle cerebral artery
occlusion (MCAO) operation at rodent model and try to rescue neurons
surrounding insult from the ischemic damage using acute transplantation of neural
stem cells (NSCs). The infarct volumes and brain atrophy were obvious diminished
at grafted NSCs treatment. The behavior recovery of grafted NSCs treatment
significantly improved compared with vehicle control. Furthermore, we detected
the inflammation related moleculars such as COX-2 and IL-1β and found that the
grfated NSCs treatment after ischemic stroke could repressed the expression of
inflammation moleculars. We also detected the protein level of heat shock protein
27 (HSP27) as a protective protein against apoptosis. The results showed that the
group of grafted NSCs treatment induced the protein level of HSP27 and down-
regulated activity of caspase-3 compared with vehicle control. Our results
demonstrate that transplanted NSCs provide benefit in behavioral function
recovery after MCAO in rats and more neuroprotection or less inflammatory
destruction. These data reveal the another essential explain of cellular
transplantation therapy in damage recovery from ischemic stroke and offer new
therapeutic possibilities.
訴求邏輯、結果闡訴、結論、英文
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The goal of this study was to screen plants for anti-allergic effects by evaluating the
amounts of histamine and leukotrienes released after guinea pig lung mast cell
activation. Bioassay-guided fractionation led to two flavonoids, baicalin from
Scutellaria baicalenesis and isoorientin from Phyllastachys edulis. Based on these
compounds, we developed two standardized extracts, SSBE from S. baicalensis and
SPEE from P. edulis. SSBE or SPEE remarkably inhibited histamine and leukotrienes
release in a dose-dependent manner. We also prepared Uniflavon™ by combining these
extracts at a 1:2 ratio; the resulting combination had a stronger activity than either
extract alone. These data suggest that Uniflavon™ in low dose than in dose of each
standardized extract alone may be utilized as a therapeutic agent not only for allergic
diseases but also for immune diseases related to mast cells, including rheumatoid
arthritis and osteoarthritis, in further studies.
Anti-allergic Flavonoids, Baicalin and Isoorientin, from Scutellaria
baicalensis and Phyllostachys edulis
論訴重點一致性