1Gene Structure 2Topics to be CoveredIntroductionGene structurePromoterTerminatorSplice site3Terminology Genome entire genetic material of an individualTranscriptome set of transcribed seuencesProteome set of proteins encoded by t!e genome"Terminology#nly one strand of $%& serves as a template for transcription'$ifferent genes are transcribed from different strands ()rom Gene to Protein*+u,aryotic Gene Structure5 - Promoter Exon1Intron1 Exon2 Terminator 3 UTR splice splice UTRtranscriptiontranslationPoly &protein-Pro,aryotic Gene StructurePromoter CDSTerminatortranscriptionGenomic $%&m.%&proteinUTRUTRtranslation/Topics to be CoveredIntroductionGene structurePromoterTerminatorSplice sites0PromoterPromoter determines11' 2!ic! strand 3ill serve as a template'2' Transcription starting point'3' Strengt! of polymerase binding'"' )reuency of polymerase binding'14Pro,aryotic Promoter#ne type of .%& polymerase'Pribno3 bo5 located at 14 6*7-bp8 3( seuence located at 73( 6*bp811+u,aryote Promoter3 types of .%& polymerases are employed in transcription of genes1.%& polymerase I transcribes r.%&.%& polymerase II transcribes all genes coding for polypeptides.%& polymerase III transcribes small cytoplasmatic .%&9 suc! as t.%&'12+u,aryote PromoterGoldberg7:ogness or T&T& located at 34&dditional regions at 144 and at 244Possible distant regions acting as en!ancers or silencers 6even more t!an (4 ,b8' 13PromoterPromoters seuences can vary tremendously'.%& polymerase recogni;es !undreds of different promoters 1"PromoterStrong promoter resemble t!e consensus seuence'