Post on 10-Apr-2020
MP BiomedicalsCulture Growth Media
www.mpbio.comMP Biomedicals Korea | 02-425-5991
Yeast 연구를 위한
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•일반 배양 배지
•선택배지 (SD, DOB, YNB, CSM)
•Yeast Transformation Kit
•Yeast Lytic Enzyme : Zymolyase
• Others
total Solution
Yeast MediaSaccharomyces cerevisiae
일반배지 (Growth Media)
YEP Broth YPD(YEPD) Agar
YPD(YEPD) Broth
1리터 제조 : 30g (6 large capsules)리터당 함량 : peptone 20g, yeast extract 10g, pH 6.5 at 30℃
형태 제품명 용량 제품번호
Powder YEP Broth 1 kg 114004032
Large Capsules YEP Broth 5 kg 114004046
1리터 제조 : 67g (67 small capsules)리터당 함량 : YPD, agar 17g
형태 제품명 용량 제품번호
Powder YPD(YEPD) Agar 1 kg 114001232
Pouches YPD(YEPD) Agar 10 × 1 L 114001275
Small Capsules YPD(YEPD) Agar 1 kg 114001231
선택배지 (Synthetic Defined Media)
SD Media (Synthetic Defined Media) SD Agar (Synthetic Defined Media Agar)
리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g,CSM-based amino acid mixture
형태 제품명 용량 제품번호
Single Dropout Formulas
Powder
SD Medium - LEU10 × 0.5 L 114811065
10 × 1 L 114811075
SD Medium - TRP 10 × 0.5 L 114812065
SD Medium - URA10 × 0.5 L 114813065
10 × 1 L 114813075
Double Dropout Formulas
Pouches SD Medium-LEU-TRP 10 × 0.5 L 114823065
Triple Dropout Formulas
PouchesSD Medium-HIS-LEU-TRP
10 × 0.5 L 114830065
4+ Dropout Formulas
PouchesSD Medium-ADE-HIS-LEU-TRP
10 × 0.5 L 114842065
리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g,CSM-based amino acid mixture, agar 17g
형태 제품명 용량 제품번호
Pouches
SDA Medium-LEU 10 × 0.5 L 114811165
SDA Medium-URA 10 × 0.5 L 114813165
SDA Medium-HIS-LEU-TRP
10 × 0.5 L 114830165
SDA Medium-LEU-TRP-URA
10 × 1 L 114833175
1리터 제조 : 50g (10 large capsules)리터당 함량 : peptone 20g, yeast extract 10g, dextrose 20g, pH 6.5 at 30℃
형태 제품명 용량 제품번호
Powder YPD(YEPD) Broth
454 g 114001022
1 kg 114001032
5 kg 114001042
Pouches YPD(YEPD) Broth 10 × 1 L 114001075
Large Capsules YPD(YEPD) Broth 1 kg 114001036
Yeast MediaSaccharomyces cerevisiae
DOB (Drop Out Base) / DOBA (Drop Out Base Agar) YNB(Yeast Nitrogen Base)
리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g, (Agar 17g)
형태 제품명 용량 제품번호
Powder
DOB Medium
227 g 114025012
454 g 114025022
1 kg 114025032
5 kg 114025042
DOB 2% Galactose (glucose-free)
454 g 114025522
DOB 2% Galactose, 1% Raffinose
227 g 114025912
DOBA Medium
454 g 114026022
1 kg 114026032
5 kg 114026042
DOBA 2% Galactose(glucose-free)
454 g 114026522
1리터 제조 : 1.7g (YNB w/ Ammonium Sulfate : 6.7g)리터당 함량 : 별도 table 참조
형태 제품명 용량 제품번호
Powder
YNB w/oAmmonium Sulfate
227 g 114027012
454 g 114027022
YNB w/Ammonium Sulfate
227 g 114027512
454 g 114027522
1 kg 114027532
5 kg 114027542
CSM (Complete Supplement Mixture)
* Single Amino Acids 용량별 구매 가능합니다.
형태 제품명 용량 제품번호
Complete Formulation
Powder CSM10 g 114500012
100 g 114500022
Single Dropout Formulation
Powder
CSM-ADE 10 g 114510012
CSM-ARG 10 g 114510112
CSM-HIS10 g 114510312
100 g 114510322
CSM-LEU10 g 114510512
100 g 114510522
CSM-LYS 10 g 114510612
CSM-MET 10 g 114510712
CSM-TRP10 g 114511012
100 g 114511022
CSM-URA10 g 114511212
100 g 114511222
Triple Dropout Formulation
Powder
CSM-HIS-LEU-TRP10 g 114530112
100 g 114530122
CSM-HIS-TRP-URA 10 g 114530812
CSM-LEU-TRP-URA 10 g 114530912
CSM-HIS-LEU-URA10 g 114531212
100 g 114531222
형태 제품명 용량 제품번호
Double Dropout Formulation
Powder
CSM-LEU-TRP10 g 114520012
100 g 114520022
CSM-HIS-TRP 10 g 114520112
CSM-LEU-URA10 g 114520212
100 g 114520222
CSM-HIS-URA10 g 114520312
100 g 114520322
CSM-HIS-LEU10 g 114520412
100 g 114520422
CSM-TRP-URA10 g 114520512
100 g 114520522
CSM-MET-URA 10 g 114520812
4+ Dropout Formulation
Powder
CSM-HIS-LEU-TRP-URA
10 g 114540012
100 g 114540022
CSM-ADE-HIS-LEU-TRP
10 g 114540412
CSM-ADE-HIS-LEU-TRP-URA
100 g 114550122
CSM-HIS-LEU-MET-TRP-URA
10 g 114550412
CSM-ADE-HIS-LEU-MET-TRP-URA
100 g 114560222
Yeast MediaSaccharomyces cerevisiae
Schizosaccharomyces pombe
기타 Media Components & Apparatus
형태 제품명 용량 제품번호
Media Components
Agar-B 454 g 113072022
Agar-Y(Superior grade for yeast cloning, growth and spheroplast regeneration)
454 g 114019022
1 kg 114019032
3-AT 50 g 114061722
5-FOA(5-Fluoroorotic Acid)
1 g 114066102
IPTG1 g 114064102
5 g 114064112
X-Gal 1 g 114063102
Yeast Extract-B454 g 113065522
1 kg 113065532
Dextrose (Glucose) 1 kg 113055032
Peptone-Y 1 kg 114018532
관련 Apparatus
Replica PlatingAppratus
100 mm 115000001
Velvet Pad, 6 Inch Square (Pack of 10)
100 mm 115000007
Replica PlatingAppratus
150 mm 115000004
Velvet Pad, 9 Inch Square (Pack of 10)
150 mm 115000009
Roll & Grow™Plating Beads
2 tubes 115000550
일반배지 (Growth Media)
EMM Broth
형태 제품명 용량 제품번호
PowderEMM Broth
227 g 114110012
454 g 114110022
1 kg 114110032
Pouches 10 × 1 L 114110075
PowderEMM w/o Dextrose
227 g 114110112
1 kg 114110132
MM w/o Nitrogen 227 g 114110712
YES Broth (Vegetative Growth)
YES Agar (Vegetative Growth)
1리터 제조 : 35g리터당 함량 : YE 5g, Dextrose 30g, 1X supplements (50mg each ADE, HIS, LEU, LYS, URA)
형태 제품명 용량 제품번호
Powder YES Broth 1 kg 114101532
1리터 제조 : 52g리터당 함량 : YES, Agar 17g
형태 제품명 용량 제품번호
Powder YES Agar 1 kg 114101732
Yeast Kits & Lytic enzyme
Yeast Transformation Kit
Yeast Lytic Enzyme
From Arthrobacter luteusUnit definition : The amount of activity causing a decrease in A
800 of 30%
Lytic Activity : 20T – 20,000 units/g 100T-100,000 units/g * This is a highly purified preparation which effectively lyses cell walls of viable yeast cells
제품명 용량 제품번호
EZ-Yeast™ Transformation Kit 200 preps 112100200
Alkali-Cation™ Yeast Transformation Kit 250 preps 112200200
제품명 용량 제품번호
Zymolyase 20T (activity : 20,000units/g) 1 g 08320921
Zymolyase 100T (activity : 100,000units/g)250 mg 08320932
500 mg 08320931
Yeast 파쇄를 위한 Beads
제품명 용량 제품번호
Lysing Matrix C(Yeast lysis 용 1mm silica beads)
50 × 2ml tubes 116912050
100 × 2ml tubes 116912100
500 × 2ml tubes 116912500
EZ-Yeast™ Transformation Kit Alkali-Cation™ Yeast Transformation Kit
• High-throughput 실험에 적합.
• Competent cell을 만들 필요 없음.
• 최소한의 hands-on time으로 간편하고 빠르게 transformation 가능
• 높은 efficiency
• Transformation 과정이 90분 이내 끝남
• 일반적인 효율은 plasmid DNA당 104transformants 이상임.
관련
제품
우수한 성능과 빠른 실험시간을직접 경험해 보고 싶으시다면,
Demo 신청 바랍니다.Lysing Matrix C
편리하고 강력한 Bead beater
FastPrep-24™
www.mpbio.com
Yeast MediaSaccharomyces cerevisiae
Synthetic defined media를 구매하는 다양한 방법Description mg/L
Potassium Phosphate monobasic 1000.000
YNBYeast Nitrogen
Base
DOBDrop Out Base
SDSyntheticDefined
Magnesium Sulfate 500.000
Sodium Chloride 100.000
Calcium Chloride 100.000
Biotin 0.002
Calcium Pantothenate 0.400
Folic Acid 0.002
Inositol 2.000
Niacin 0.400
p-Aminobenzoic Acid 0.200
Pyridoxine HCl 0.400
Riboflavin 0.200
Thiamine HCl 0.400
Boric Acid 0.500
Copper Sulfate 0.040
Potassium Iodide 0.100
Ferric Chloride 0.200
Manganese Sulfate 0.400
Sodium Molybdate 0.200
Zinc Sulfate 0.400
Total 1705.844
Ammonium Sulfate 5000.000
Dextrose 20000.000
Total 26705.844
Adenine 10.000
CSMComplete Supplement Mixture
L-Arginine HCl 50.000
L-Aspartic Acid 80.000
L-Histidine HCl 20.000
L-Isoleucine 50.000
L-Leucine 100.000
L-Lysine HCl 50.000
L-Methionine 20.000
L-Phenylalanine 50.000
L-Threonine 100.000
L-Tryptophan 50.000
Uracil 20.000
L-Tyrosine 50.000
L-Valine 140.000
Total 790.000
Total 27495.844