One Call Yeast · 2014-03-24 · Yeast Kits & Lytic enzyme Yeast Transformation Kit Yeast Lytic...

MP BiomedicalsCulture Growth Media

www.mpbio.comMP Biomedicals Korea | 02-425-5991

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•일반 배양 배지

•선택배지 (SD, DOB, YNB, CSM)

•Yeast Transformation Kit

•Yeast Lytic Enzyme : Zymolyase

• Others

total Solution

Yeast MediaSaccharomyces cerevisiae

일반배지 (Growth Media)

YEP Broth YPD(YEPD) Agar

YPD(YEPD) Broth

1리터 제조 : 30g (6 large capsules)리터당 함량 : peptone 20g, yeast extract 10g, pH 6.5 at 30℃

형태 제품명 용량 제품번호

Powder YEP Broth 1 kg 114004032

Large Capsules YEP Broth 5 kg 114004046

1리터 제조 : 67g (67 small capsules)리터당 함량 : YPD, agar 17g


Powder YPD(YEPD) Agar 1 kg 114001232

Pouches YPD(YEPD) Agar 10 × 1 L 114001275

Small Capsules YPD(YEPD) Agar 1 kg 114001231

선택배지 (Synthetic Defined Media)

SD Media (Synthetic Defined Media) SD Agar (Synthetic Defined Media Agar)

리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g,CSM-based amino acid mixture


Single Dropout Formulas

Powder

SD Medium - LEU10 × 0.5 L 114811065

10 × 1 L 114811075

SD Medium - TRP 10 × 0.5 L 114812065

SD Medium - URA10 × 0.5 L 114813065

10 × 1 L 114813075

Double Dropout Formulas

Pouches SD Medium-LEU-TRP 10 × 0.5 L 114823065

Triple Dropout Formulas

PouchesSD Medium-HIS-LEU-TRP

10 × 0.5 L 114830065

4+ Dropout Formulas

PouchesSD Medium-ADE-HIS-LEU-TRP

10 × 0.5 L 114842065

리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g,CSM-based amino acid mixture, agar 17g


Pouches

SDA Medium-LEU 10 × 0.5 L 114811165

SDA Medium-URA 10 × 0.5 L 114813165

SDA Medium-HIS-LEU-TRP

10 × 0.5 L 114830165

SDA Medium-LEU-TRP-URA

10 × 1 L 114833175

1리터 제조 : 50g (10 large capsules)리터당 함량 : peptone 20g, yeast extract 10g, dextrose 20g, pH 6.5 at 30℃


Powder YPD(YEPD) Broth

454 g 114001022

1 kg 114001032

5 kg 114001042

Pouches YPD(YEPD) Broth 10 × 1 L 114001075

Large Capsules YPD(YEPD) Broth 1 kg 114001036


DOB (Drop Out Base) / DOBA (Drop Out Base Agar) YNB(Yeast Nitrogen Base)

리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g, (Agar 17g)


Powder

DOB Medium

227 g 114025012

454 g 114025022

1 kg 114025032

5 kg 114025042

DOB 2% Galactose (glucose-free)

454 g 114025522

DOB 2% Galactose, 1% Raffinose

227 g 114025912

DOBA Medium

454 g 114026022

1 kg 114026032

5 kg 114026042

DOBA 2% Galactose(glucose-free)

454 g 114026522

1리터 제조 : 1.7g (YNB w/ Ammonium Sulfate : 6.7g)리터당 함량 : 별도 table 참조


Powder

YNB w/oAmmonium Sulfate

227 g 114027012

454 g 114027022

YNB w/Ammonium Sulfate

227 g 114027512

454 g 114027522

1 kg 114027532

5 kg 114027542

CSM (Complete Supplement Mixture)

* Single Amino Acids 용량별 구매 가능합니다.


Complete Formulation

Powder CSM10 g 114500012

100 g 114500022

Single Dropout Formulation

Powder

CSM-ADE 10 g 114510012

CSM-ARG 10 g 114510112

CSM-HIS10 g 114510312

100 g 114510322

CSM-LEU10 g 114510512

100 g 114510522

CSM-LYS 10 g 114510612

CSM-MET 10 g 114510712

CSM-TRP10 g 114511012

100 g 114511022

CSM-URA10 g 114511212

100 g 114511222

Triple Dropout Formulation

Powder

CSM-HIS-LEU-TRP10 g 114530112

100 g 114530122

CSM-HIS-TRP-URA 10 g 114530812

CSM-LEU-TRP-URA 10 g 114530912

CSM-HIS-LEU-URA10 g 114531212

100 g 114531222


Double Dropout Formulation

Powder

CSM-LEU-TRP10 g 114520012

100 g 114520022

CSM-HIS-TRP 10 g 114520112

CSM-LEU-URA10 g 114520212

100 g 114520222

CSM-HIS-URA10 g 114520312

100 g 114520322

CSM-HIS-LEU10 g 114520412

100 g 114520422

CSM-TRP-URA10 g 114520512

100 g 114520522

CSM-MET-URA 10 g 114520812

4+ Dropout Formulation

Powder

CSM-HIS-LEU-TRP-URA

10 g 114540012

100 g 114540022

CSM-ADE-HIS-LEU-TRP

10 g 114540412

CSM-ADE-HIS-LEU-TRP-URA

100 g 114550122

CSM-HIS-LEU-MET-TRP-URA

10 g 114550412

CSM-ADE-HIS-LEU-MET-TRP-URA

100 g 114560222


Schizosaccharomyces pombe

기타 Media Components & Apparatus


Media Components

Agar-B 454 g 113072022

Agar-Y(Superior grade for yeast cloning, growth and spheroplast regeneration)

454 g 114019022

1 kg 114019032

3-AT 50 g 114061722

5-FOA(5-Fluoroorotic Acid)

1 g 114066102

IPTG1 g 114064102

5 g 114064112

X-Gal 1 g 114063102

Yeast Extract-B454 g 113065522

1 kg 113065532

Dextrose (Glucose) 1 kg 113055032

Peptone-Y 1 kg 114018532

관련 Apparatus

Replica PlatingAppratus

100 mm 115000001

Velvet Pad, 6 Inch Square (Pack of 10)

100 mm 115000007

Replica PlatingAppratus

150 mm 115000004

Velvet Pad, 9 Inch Square (Pack of 10)

150 mm 115000009

Roll & Grow™Plating Beads

2 tubes 115000550

일반배지 (Growth Media)

EMM Broth


PowderEMM Broth

227 g 114110012

454 g 114110022

1 kg 114110032

Pouches 10 × 1 L 114110075

PowderEMM w/o Dextrose

227 g 114110112

1 kg 114110132

MM w/o Nitrogen 227 g 114110712

YES Broth (Vegetative Growth)

YES Agar (Vegetative Growth)

1리터 제조 : 35g리터당 함량 : YE 5g, Dextrose 30g, 1X supplements (50mg each ADE, HIS, LEU, LYS, URA)


Powder YES Broth 1 kg 114101532

1리터 제조 : 52g리터당 함량 : YES, Agar 17g


Powder YES Agar 1 kg 114101732

Yeast Kits & Lytic enzyme

Yeast Transformation Kit

Yeast Lytic Enzyme

From Arthrobacter luteusUnit definition : The amount of activity causing a decrease in A

800 of 30%

Lytic Activity : 20T – 20,000 units/g 100T-100,000 units/g * This is a highly purified preparation which effectively lyses cell walls of viable yeast cells

제품명 용량 제품번호

EZ-Yeast™ Transformation Kit 200 preps 112100200

Alkali-Cation™ Yeast Transformation Kit 250 preps 112200200


Zymolyase 20T (activity : 20,000units/g) 1 g 08320921

Zymolyase 100T (activity : 100,000units/g)250 mg 08320932

500 mg 08320931

Yeast 파쇄를 위한 Beads


Lysing Matrix C(Yeast lysis 용 1mm silica beads)

50 × 2ml tubes 116912050

100 × 2ml tubes 116912100

500 × 2ml tubes 116912500

EZ-Yeast™ Transformation Kit Alkali-Cation™ Yeast Transformation Kit

• High-throughput 실험에 적합.

• Competent cell을 만들 필요 없음.

• 최소한의 hands-on time으로 간편하고 빠르게 transformation 가능

• 높은 efficiency

• Transformation 과정이 90분 이내 끝남

• 일반적인 효율은 plasmid DNA당 104transformants 이상임.

관련

제품

우수한 성능과 빠른 실험시간을직접 경험해 보고 싶으시다면,

Demo 신청 바랍니다.Lysing Matrix C

편리하고 강력한 Bead beater

FastPrep-24™

www.mpbio.com


Synthetic defined media를 구매하는 다양한 방법Description mg/L

Potassium Phosphate monobasic 1000.000

YNBYeast Nitrogen

Base

DOBDrop Out Base

SDSyntheticDefined

Magnesium Sulfate 500.000

Sodium Chloride 100.000

Calcium Chloride 100.000

Biotin 0.002

Calcium Pantothenate 0.400

Folic Acid 0.002

Inositol 2.000

Niacin 0.400

p-Aminobenzoic Acid 0.200

Pyridoxine HCl 0.400

Riboflavin 0.200

Thiamine HCl 0.400

Boric Acid 0.500

Copper Sulfate 0.040

Potassium Iodide 0.100

Ferric Chloride 0.200

Manganese Sulfate 0.400

Sodium Molybdate 0.200

Zinc Sulfate 0.400

Total 1705.844

Ammonium Sulfate 5000.000

Dextrose 20000.000

Total 26705.844

Adenine 10.000

CSMComplete Supplement Mixture

L-Arginine HCl 50.000

L-Aspartic Acid 80.000

L-Histidine HCl 20.000

L-Isoleucine 50.000

L-Leucine 100.000

L-Lysine HCl 50.000

L-Methionine 20.000

L-Phenylalanine 50.000

L-Threonine 100.000

L-Tryptophan 50.000

Uracil 20.000

L-Tyrosine 50.000

L-Valine 140.000

Total 790.000

Total 27495.844

One Call Yeast · 2014-03-24 · Yeast Kits & Lytic enzyme Yeast Transformation Kit Yeast Lytic...

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Transcript of One Call Yeast · 2014-03-24 · Yeast Kits & Lytic enzyme Yeast Transformation Kit Yeast Lytic...