Astaxanthin from yeast affect on increasing the red color rate of male Guppy

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Transcript of Astaxanthin from yeast affect on increasing the red color rate of male Guppy

astaxanthin

astaxanthin

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: Poecilia reticulata 3 -5 Family : Poecidae

()

() Phaffia rhodozyma

() astaxanthin

astaxanthin Phaffia rhodozyma

Glucose 250 NUNCLON DELTA(250 ml Tissue Culture Flask) 10 NALGENE U.S.A. (Oak Ridge Centrifuge tube 10 ml)

()NOKIA(Nokia N70)HIRAYAMA, JAPAN (Autoclave) CECIL(Centrifuge) SCIENCETECH COMPANY(Incubator)Incubator ShakerPANAPOLY TECH

Phaffia rhodozyma PDB 1 250 PDB 150

() SHAKER 150 30 7

() PDB 10 3000 15 3000 15

() astaxanthin 50, 100 200 60 24

() 180 12 15 1 2, 3 4 astaxanthin 50, 100 200 3 21 7

() MVH PC image V.8 %R, %G %B R G B E = (R2 + G 2 + B2)1/2 E R 255 G0 B0

() (E) E = (R2 + G2 + B2)1/2

DR = (R R standard) R = %R x 255DG = (G G standard) G = %G x 255DB = (B B standard) B = %B x 255R standard = R = 255G standard = G = 0B standard = B = 0

( Phaffia rhodozyma ) 0.33

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1( Phaffia rhodozyma 50 ) 0.43

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2 ( Phaffia rhodozyma 100 ) 0.57

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astaxanthin astaxanthin

Phaffia rhodozyma Penaeus monodon Fabricius. []. http://shrimpbase.fish.ku.ac.th/display_abs_t.php?id=176 . 2541. Astaxanthin Haematococus sp. () Astaxanthin Phaffia Rhodozyma []. http://www.kmutt.ac.th/rippc/biomass.htm Phaffia rhodozyma []. http://www.scisoc.or.th/stt/33/sec_b/paper/stt33_B4_B0019.pdfEffect of Feeding Methods on the Astaxanthin Production by in fed-batch Process Phaffia Rhodozyma [On-line]. Available: http://www.scielo.br/pdf/babt/v48n3/24762.pdf

()Andrewes et al.1976. (3R,3R)-Astaxanthin From The Yeast Phaffia rhodozyma, Phytochemistry 15:1009-1011Gio-Bin Lim, Sang-Yun Lee, Eun-Kyu Lee, Seung-Joo Haam and Woo-Sik Kim. (2002). Separation of Astaxanthin from red yeast Phaffia Rhodozyma by supereritical carbon dioxide extraction. Biochemical Engineering Journal, 11: 181-187.Guppy [On-line]. Available: http://www.petco.com/caresheets/fish/Guppy.pdfVerlhac V,NDoye A, Gabaudan J, Troutaud D, Deschaux P. 1991. Vitamin nutrition and fish immunity: influence of antioxidant vitamins (C and E) on immune response of rainbow trout. En Kaushik SJ, Luquet P (Eds.) Fish Nutrition in Practic. INRA. Paris, Francia. pp. 167-177EricA.Johnson. Phaffia rhodozyma : colorful odyssey. Microbiol(2003) 6:169-174.