Title セラミック水の Streptococcus mutans におけるう蝕原性抑制効果の検討
Author(s) 米山, 莉紗
Citation
Issue Date
Text Version ETD
URL https://doi.org/10.18910/72249
DOI 10.18910/72249
rights
Copyright: All rights reserved. No part of thispublication may be translated into otherlanguages, reproduced or utilized in any form orby any means, electronic or mechanical,including photocopying, recording, microcopying,or by any information storage and retrievalsystem, without permission in writing from thepublisher.
Note
Osaka University Knowledge Archive : OUKAOsaka University Knowledge Archive : OUKA
https://ir.library.osaka-u.ac.jp/repo/ouka/all/
Osaka University
Streptococcus mutans
1
6074-
-
Streptococcus mutans
Hamada Slade, 1980 S. mutans
, 1997 S. mutans
190 kDa Protein
antigen PA Okahashi , 1989
S. mutans 3 Glucosyltransferase ; GTF GTFB
GTFCGTFD
Ooshima , 2001 S. mutans
Ooshima , 2001 S. mutans
Glucan-binding protein ; Gbp GTF
S. mutans Banas
, 2003
S. mutans
Hamada Slade, 1980Hamada , 1984
Cnm Cbm
CBP S. mutans
Nomura , 2012
Smith , 2002
Matsumoto , 1999Signoretto , 2010
Smith , 2002
Gluzman , 2013
2
He , 2015
β-TCP
Myoui , 2006
3,000
, 2016
S. mutans
in vitro S. mutans
-
-
-
3
��3��-
-
LK ?GE@=>5��
1
3,000
wt% 76 : 15 : 9
2 100 mg
1 1 L 1
0.1 ppm 3
H2O
45 NaCl
NaCl 5.0 wt %
10 6
S. mutans
8
MK ��/2%�
S. mutans MT8148
c CBP PA Ooshima 1983
SA31 k Cbm PA Nakano 2008
Streptococcus sobrinus 6715 g Ooshima 1993
Brain Heart Infusion BHI; Difco Laboratories Detroit, MO, USA
Todd Hewitt TH; Difco Laboratories Mitis Salivarius MS
Difco Laboratories, Detroit, MO, USA 0.2 U/mL; Sigma-Aldrich
Co., St. Louis, MO, USA 15 % w/v MSB
BHI TH 37 18
MSB 95 % 5% 37 48
4
NK �%��4,18��
Sasaki 2004 10 mL
BHI 37 18 MT8148 SA31 6715
3,000 rpm 10
Phosphate buffered saline; PBS OD550 = 1.0
1.0×109 CFU/mL
37 5 15 30 45 1.5 3 6
12 1 ppm10 ppm
100 ppm MSB
37 48 %
S. mutans /
S. mutans 5
OK ������4,18��
Sasaki 2004 10 mL
BHI TH 37 18 MT8148
SA31 OD550 = 1.0 1.0×109 CFU/mL
1.0×105 CFU
100 ppm 37 3
6 12 MSB
37 48
% S. mutans /
S. mutans
5
PK >=IJ>�� ��#418����5��
Kawabata Hamada 1999
10 mL BHI 37 18 MT8148
SA31 6715 3,000 rpm 10
1% BHI BHI
5
1/10 BHI 1.0×107 CFU/ml 30°
37 18
3
3 mL
Libra S12, Biochrom Ltd, Cambridge, UK
OD550 %
OD550 / OD550 5
QK B;<D:HF��#418����5��
Kuboniwa 2006
10 mL BHI 37 18 MT8148
3,000 rpm 10 10 mM Hexidium Iodide
Molecular Probes, Eugene, OR, USA 5 µL 1 mL
15 100 ppm
0.25 % BHI BHI
1/10 BHI OD600 0.1 1
60 30 12,000 rpm 1
25 %
Chambered Coverglass System CultureWell™,Grace Bio Lab, Bend, OR, USA 150
µL 37 2
200 µL 37
24 PBS 100 µL
488 nm
TSC-SP5 Leica Microsystems GmbH, Wetzlar, Germany
DMI6000B Leica 63×
Image J version 1.34s National Institutes of Health, Bethesda, MD, USA
Ardin 2014 10
mL BHI 37 18 MT8148 6715
3,000 rpm 10 100 ppm
0.25 % BHI OD600 0.1
6
60 30
12,000 rpm 1
25 % 0.45 µL
96 Corning Inc., Corning, NY, USA 200 µL
37 2
200 µL 37 24
PBS 0.05 %
Sigma-Aldrich 200 µL 5
PBS
95 %
Thermo Fisher Scientific, Waltham, MA, USA OD595
5
RK .&����4,18CA9*3/2$���
H26-E31
7
41 17 24 18 37 22.1
MSB
37 48
S. mutans 1×103 CFU/mL 14
27 13 14 18 37
23.2
Ooshima 1994
MSB 37 48
Quiugley Hein
1962 Red-
Cote, John O.Butler Co., Chicago, IL, USA
0 3 4
2
1 10
7
1
1
S. mutans
SK !'��
GraphPad Prism 6 GraphPad Software Inc., La Jolla, CA, USA
ANOVA post-hoc Bonferroni
5 %
8
�
LK�%��4,18��
1.0×109 CFU/mL S. mutans MT8148 100 ppm
1.5 5 % 3
8 PBS 1.5
9 S. mutans SA31
1.5
3 20 % 6
0 % 10 PBS
1.5 10 %
11
MT8148 5 50 %
90 12
MT8148 1.5
10 ppm P 0.05 13 S. sobrinus
6715 5
3 0 % 14
MK ������4,18���100 ppm BHI 1.0×105 CFU MT8148
SA31 3 6 12
S. mutans
1516 TH
S. mutans 1718
NK>=IJ>�� ��#418����5��
BHI 1 % BHI 1.0×107 CFU/mL
MT8148 100 ppm
19
BHI 1/10 1 % BHI
P
9
0.001 20 6715 BHI
P 0.05
2122
OKB;<D:HF��#418����5��
BHI 0.25 %
BHI MT8148 100 ppm
MT8148
23 BHI 1/10
BHI MT8148
6715
P 0.001 2425
PK.&����4,18$���
1
2627 100 ppm
1
P 0.05 2628
S. mutans 1 S. mutans
S. mutans
P 0.05 29
-
-
-
-
10
"-
-
TiC
64:36 1 0.08 ppm
, 2016 TiO2
Lu , 2003
Yao , 2008
Burne
, 1987
Burne , 1987Bellantone , 2002Hipler ,
2006 S. mutans
Burne , 1987
Castillo ,
2011
Kawasita , 2000
H2O2 UV
Porphyromonas gingivalis Fusobacterium nucleatum S. mutans
Feuerstein , 2006Ikai , 2013
11
MT8148 SA 31 MT8148
S. mutans
c/e/f/k
Hamada , 1980Nakano , 2004 S. sobrinus
d/g Okahashi , 1986 MT8148
SA31 c k
MT8148
SA31
Nomura , 2012 SA 31 MT8148
S. mutans
SA31 PBS
PBS
0.1 ppm
NaCl
5.0 wt % 1 ppm
Cl- AgCl
NaCl
10 SA31
PBS
, 2016
PBS
BHI
S. mutans S. sobrinus
12
S. mutans S. sobrinus
BHI
BHI
BHI
S. mutans
99.5 %
0.5 % Na+ K+
Ca2+ Cl-HCO3- Hofman
, 2001Kaufman , 2002
S. mutans
Smith , 2002
Ooshima , 1994
S. mutans S. mutans
S.
mutans Ooshima , 1994
S. mutans
S. mutans
Hamada , 1984
Moriwaki , 2011
13
TiC
BHI
S. mutans
S. mutans
14
(-
PBS
S. mutans
BHI S. mutans
S. mutans
S. mutans
15
)+-
16
��-
-
, . 1997. .
. , , , , , pp.156-
171, , .
Banas JA, Vickerman MM. 2003. Glucan-binding proteins of the oral streptococci. Crit rev Oral
Biol Med. 14, 89-99.
Bellantone M, Williams HD, Hench LL. 2002. Broad-spectrum bactericidal activity of Ag(2)O-
doped bioactive glass. Antimicrob Agents Chemother. 46, 1940–1945.
Burne RA, Schilling K, Bowen WH, Yasbin RE. 1987. Expression, purification, and
characterization of an exo-beta-D-fructosidase of Streptococcus mutans. J Bacteriol. 169,
4507-4517.
Castillo J, Rivera S, Aparicio T, Lazo R, Aw TC, Mancl L, Milgrom P. 2011. The short-term
effects of diammine silver fluoride on tooth sensitivity: a randomized controlled trial. J Dent
Res. 90, 203-208.
Feuerstein O, Ginsburg I, Dayan E, Veler D, Weiss EI. 2005. Mechanism of visible light
phototoxicity on Porphyromonas gingivalis and Fusobacterium nucleatum. Photochem
Photobiol. 81, 1186-1189.
Feuerstein O, Moreinos D, Steinberg D. 2006. Synergic antibacterial effect between visible
light and hydrogen peroxide on Streptococcus mutans. J Antimicrob Chemother. 57, 872-
876.
Gluzman R, Katz RV, Fray BJ, McGowan R. 2013. Prevention of root Caries: a literature
review of primary and secondary preventive agents. Spec Care Dent. 33, 133-140.
Hamada S, and Slade HD. 1980. Biology, immunology, and cariogenicity of Streptococcus
mutans. Microbiol Rev. 44, 331-384.
Hamada S, Koga T, Ooshima T. 1984. Virulence factors of Streptococcus mutans and dental
caries prevention. J Dent Res. 63, 407-411.
He J, Zhu X, Qi Z, Wang C, Mao X, Zhu C, He Z, Li M, Tang Z. 2015. Killing dental
pathogens using antibacterial graphene oxide. ACS Appl Mater Interfaces. 7, 5605-5611.
Hipler UC, Elsner P, Fluhr JW. 2006. Antifungal and antibacterial properties of a silver-loaded
cellulosic fiber. J Biomed Mater Res B Appl Biomater. 77, 156-163.
Hofman LF. 2001. Human saliva as a diagnostic specimen. J Nutr. 131, 162S-165S.
Ikai H, Nakamura K, Kanno T, Shirato M, Meirelles L, Sasaki K, Niwano Y. 2013.
17
Synergistic effect of proanthocyanidin on the bactericidal action of the photolysis of H2O2.
Biocontrol Sci. 18, 137-141.
Kaufman E, Lamster IB. 2002. The diagnostic applications of saliva - a review. Crit Rev Oral
Biol Med. 13, 197-212.
Kawabata S, Hamada S. 1999. Studying biofilm formation of mutans streptococci. Methods
Enzymol. 310, 513-523.
Kawashita M, Tsuneyama S, Miyaji F, Kokubo T, Kozuka H, Yamamoto K. 2000.
Antibacterial silver-containing silica glass prepared by sol-gel method. Biomaterials. 21,
393-398.
Kuboniwa M, Tribble GD, James CE, Kilic AO, Tao L, Herzberg MC, Shizukuishi S,
Lamont RJ. 2006. Streptococcus gordonii utilizes several distinct gene functions to recruit
Porphyromonas gingivalis into a mixed community. Mol Microbiol. 60, 121-139.
Lu ZX, Zhou L, Zhang ZL, Shi WL, Xie ZX, Xie HY, Pang DW, Shen P. 2003. Cell damage
induded by photocatalysis of Tio2 thin films. Langmuir. 19, 8765-8768.
Matsumoto M, Minami T, Sasaki H, Sobue S, Hamada S, Ooshima T. 1999. Inhibitory
effects of oolong tea extract on caries-inducing properties of mutans streptococci. Caries Res.
33, 441-445.
Myoui A, Nishikawa M, Fujimmoto T, Kizawa T, Yamazaki N, Kakunaga S, Yoshikawa H.
2006. Treatment for Bone Defects –Bone substitutes and bone tissue engineering using
mesenchymal stem cells. JSMBE. 44, 530-536.
Nakano K, Nomura R, Nakagawa I, Hamada S, Ooshima T. 2004. Demonstration of
Streptococcus mutans with a cell wall polysaccharide specific to a new serotype, k, in the
human oral cavity. J Clin Microbiol. 58, 469-475.
Nakano K, Nomura R, Nakagawa I, Hamada S, Ooshima T. 2005. Role of glucose side chains
with serotype-specific polysaccharide in the cariogenicity of Streptococcus mutans. Caries
Res. 39, 262-268.
Nakano K, Nomura R, Nemoto H, Lapirattanakul J, Taniguchi N, Grönroos L, Alaluusua
S, Ooshima T. 2008. Protein antigen in serotype k Streptococcus mutans clinical isolates. J
Dent Res. 87, 964-968.
Nomura R, Nakano K, Naka S, Nemoto H, Masuda K, Lapirattanakul J, Alaluusua S,
Matsumoto M, Kawabata S, Ooshima T. 2012. Identification and characterization oh a
collagen-binding-protein, Cbm, in Streptococcus mutans. Mol. Oral Microbiol. 27, 308-323.
Okahashi N, Koga T, Hamada S. 1986. Purification and immunochemical properties of a protein
18
antigen from serotype g Streptococcus mutans. Microbiol. Immunol. 30, 35-47.
Okahashi N, Sasakawa C, Yoshikawa M, Hamada S, Koga T. 1989.Molecular characterization
of a surface protein antigen gene serotype c Streptococcus mutans implicated in dental caries.
Mol Microbial. 27,308-323.
Ooshima T, Izumitani A, Sobue S, Hamada S. 1983. Cariostatic effect of palatinose on
experimental dental caries in rats. Jpn. J Med Sci Biol. 36, 219-223.
Ooshima T, Matsumura M, Hoshino T, Kawabvata S, Sobue S, Fujiwara T. 2001.
Contributions of three glycosyltransferases to sucrose-dependent adherence of Streptococcus
mutans. J Dent Res. 80, 1672-1677.
Ooshima T, Minami T, Aono W, Izumitani A, Sobue S, Fujiwara T, Kawabata S, Hamada
S. 1993. Oolong tea polyphenols inhibit experimental dental caries in SPF rats infected with
humans streptococci. Caries Res.27,124-129.
Ooshima T, Minami T, Aono W, Tamura Y, Hamada S. 1994. Reduction of dental plaque
deposition in humans by oolong tea extract. Caries Res. 28, 146-149.
Quigley GA and Hein JW. 1962. Comparative cleansing efficiency of manual and power
brushing. J Am Dent Assoc. 25, 26-29.
Sasaki H, Matsumoto M, Tanaka T, Maeda M, Nakai M, Hamada S, Ooshima T. 2004.
Antibacterial activity of polyphenol components in oolong tea extract against Streptococcus
mutans. Caries Res. 38, 2-8.
Signoretto C, Bianchi F, Burlacchini G, Sivieri F, Spratt D, Canepari P. 2010. Drinking
habits are associated with changes in the dental plaque microbial community. J Clin
Microbiol. 48, 347-356.
Smith DM, Wang Z, Kazi A, Li LH, Chan TH, Dou QP. 2002. Synthetic analogs of green tea
polyphenols as proteasome inhibitors. Mol Med. 8, 382-392.
Yao Y, Ohko Y, Sekiguchi Y, Fujishima A, Kubota Y. 2008. self-sterilization using silicone
catheters coatedwith Ag and TiO2 nanocomposite thin films. J Biomed Mater Res B Appl
Biomater. 85, 453-460.
. .
WO 2016/111285. (2016.7.14).
-ł b��û�ì÷ġĴİĤĚĠĀ�O\r!c[ûëčģĢĹľĘĺįĹîĊĂÂĐv&ôľĮķĤĥ�ÿĭķĠGOô÷ŀ
òĀĮķĤĥÿ�~õčüľ��&O"Kð¶[Đ�ôúª��ÿ�WôľZ�ûÂ�Y��ģĢĹġĴİĤĚĠðIċĎčŀV�ŋgF�ėĺĕĠijĺ
�~ "K �&�
-Ń b��û�ì÷ġĴİĤĚĠġĴİĤĚĮķĤĥā7;¹ûľġĴİĤĚýíôð®�ô÷��þ�k�¢�i½Đ�õŀ
ġĴİĤĚĮķĤĥĐ��õčòüÿĊČġĴİĤĚ�aĐI÷ŀ
ġĴİĤĚĮķĤĥ
ġĴİĤĚ�a
Í*�N
-ń ġĴİĤĚĮķĤĥĐp�ÿQ�ô÷ÈĀÂēėĹ}EġĴİĤĚĮķĤĥĐp¿p�ÿQ�ôy=õčüľÂēėĹĀ}Eā� 1 _Äû 0.08 ppm �»ąû�^õčŀÆ 0.1 ppm �»ûÍ*�NĐ¡Sõčŀ¬�pÿQ�ô÷2&Ĉ'jĀ eĐ�ô÷ŀ
0.1
0.08
0.06
0.04
0.02
(ppm)
0
�����
���
1 ��
OH OH
CH3
CH3
CH3
CH3
CH3
CH3
-Ņ p�ûġĴİĤĚĠïċX�óĎčĴğĘĶÉ:ĠīĹ�ϳ¥ĻElectron Spin ResonanceŌESRļĐ�ìúĴğĘĶĐ�dô÷ŀġĴİĤĚĮķĤĥĐp�ÿQ�õčüľIJģĶĴğĘĶĻßàÚļüĪĦĸęĞĴğĘĶĻâàļð��õčŀ
OHOH
-ņ p�ûġĴİĤĚĠïċX�óĎčĴğĘĶÉ:ĠīĹ�ϳ¥ĻESRļĐ�ìúp�ÿX�óĎčĴğĘĶĐ�dô÷ŀÝŋ��üģĢĹĐ��&Oóö÷ĮķĤĥĻTiCļûāľĪĦĸęĞĴğĘĶĀ��ð·ćċĎčŀÞŋ��ľģĢĹîĊĂÂĐ��&Oóö÷ĮķĤĥĻTiC+AgļûāľIJģĶĴğĘĶüĪĦĸęĞĴğĘĶĀ��ð·ćċĎčŀßŋpĀĆĀ1zsGŀ
Þ
ß
Ý
OH OH OH OH
CH3
CH3
CH3 CH
3 CH3
CH3
-Ň ġĴİĤĚĮķĤĥĐ%�}EĀÌ3p�ÿQ�ô÷ÈĀÂēėĹ}EġĴİĤĚĮķĤĥĐÌ3p�ÿQ�õčüľáãßä }Eüo�ôÂēėĹĀ}Eā�^õčŀ áãßä }E ÛÖ×Ñèç ÒÑĀ2&ľQ�HÜÙÑ_Äû ØÑææåÑ�ąû�^ô÷ŀ
1
(ppm)
0.1
0.01
�
��
���
����
#� !)(�����
������������
%'*&$"������
��� ���)(����������� ���)(����������� ���)(����������� ���)(��������
������
0
41(Ā²Î¦Ļ�L 17(ê8L 24(ê18-37lêB/CÐ 22.1lļ
ġĴİĤĚpûnÌHíðìĻ1]ń,%łŁ��ļ
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
İĨĴĶĔĖĺĢĺûnÌHíðìĻ1]ń,%łŁ��ļ
ł¾ÄĀēĹĢĺĩĶ
ł]�
ň]�
łŅ]�
Ńł]�
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
27(Ļ�L 13(ê8L 14(ê18-37lêB/CÐ 23.2lļ
ŃěĶĺĭÿTČ�ñ
Ç6 14(Ļ+u�ÿ)ąĎč S.mutansĀĜĸħĺZð 1é103 CFU/mL�ĀĈĀļ
-ň íP��eÿÅõčĪĥĐ>¸üô÷©D��ĀtĎ
ġĴİĤĚpûnÌHíðìĻ1]ń,%łŁ��ļ
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
İĨĴĶĔĖĺĢĺûnÌHíðìĻ1]ń,%łŁ��ļ
ł¾ÄĀēĹĢĺĩĶ
ł]�
ň]�
łŅ]�
Ńł]�
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
+uU#ĻS. mutansZļĭĴĺĚĠĜđµf
n=14n=13
*-,+()#%'�$ ���/��1,.Q0�
*-,+()#%'�$ ���/��1PBS0�
100
40
20
(%)
0
80
60
0� 1.5� 6� 12�
MT8148�
���$���
�����������
***� ***�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
MT8148�
���$���
�����������
***� ***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�***� ***�
***�***�
***� ***�
***�
*�
**�
**�
���0��$��"�� !�������& /Student t �� *P<0.05, **P<0.01, ***P<0.0010�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
���0��$��"�� !�������& /Student t �� **P<0.01, ***P<0.0010�
3�
-ʼn ġĴİĤĚpÿĊč«ĀR«�eĻ{9ŋ|«¬�pļMT8148 gÿġĴİĤĚpĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷ŀġĴİĤĚpw�H 1.5 _Äû«Ā�&ā 5 % �ąûx?ô÷ŀĻ***P�0.001 ANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
*-,+()#%'�$ ���/��1,.Q0�
*-,+()#%'�$ ���/��1PBS0�
100
40
20
(%)
0
80
60
0� 1.5� 6� 12�
MT8148�
���$���
�����������
***� ***�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
MT8148�
���$���
�����������
***� ***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�***� ***�
***�***�
***� ***�
***�
*�
**�
**�
���0��$��"�� !�������& /Student t �� *P<0.05, **P<0.01, ***P<0.0010�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
���0��$��"�� !�������& /Student t �� **P<0.01, ***P<0.0010�
3�
-Ŋ ġĴİĤĚpÿĊč«ĀR«�eĻ{9ŋĵĹÀ¤°��Ì3pļMT8148 gÿġĴİĤĚpĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷ŀġĴİĤĚpw�H 1.5 _Äû«Ā�&ā㥠0 % ąûx?ô÷ŀĻ***P�0.001, ANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
*-,+()#%'�$ ���/��1,.Q0�
*-,+()#%'�$ ���/��1PBS0�
100
40
20
(%)
0
80
60
0� 1.5� 6� 12�
MT8148�
���$���
�����������
***� ***�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
MT8148�
���$���
�����������
***� ***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�***� ***�
***�***�
***� ***�
***�
*�
**�
**�
���0��$��"�� !�������& /Student t �� *P<0.05, **P<0.01, ***P<0.0010�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
���0��$��"�� !�������& /Student t �� **P<0.01, ***P<0.0010�
3�
-łŁ ġĴİĤĚpÿĊč«ĀR«�eĻ{9ŋ|«¬�pļSA31 gÿġĴİĤĚpĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷ŀġĴİĤĚpw�H 1.5 _Äû«Ā�&ā ��ąûx?ôľ6 _ÄHÿā㥠0 % ąûx?ô÷ŀÓ*P�0.05, **P�0.01, ***P<0.001, ANOVA ĀH post-hoc ´düôúBonferroni rÿĊČh=ļ
*-,+()#%'�$ ���/��1,.Q0�
*-,+()#%'�$ ���/��1PBS0�
100
40
20
(%)
0
80
60
0� 1.5� 6� 12�
MT8148�
���$���
�����������
***� ***�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
MT8148�
���$���
�����������
***� ***�
100
40
20
(%)
0
80
60
0� 1.5� 3� 6� 12�
SA31�
���$���
�����������
***�***� ***�
***�***�
***� ***�
***�
*�
**�
**�
���0��$��"�� !�������& /Student t �� *P<0.05, **P<0.01, ***P<0.0010�
���0��$��"�� !�������& /Student t �� ***P<0.0010�
���0��$��"�� !�������& /Student t �� **P<0.01, ***P<0.0010�
3�
-łł ġĴİĤĚpÿĊč«ĀR«�eĻ{9ŋĵĹÀ¤°��Ì3pļSA31 gÿġĴİĤĚpĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷ŀġĴİĤĚpw�H 1.5 _Äû«Ā�&ā 20 %�ąûx?ôľ3 _ÄHÿā㥠0 % ąûx?ô÷ŀ(**P�0.01,***P�0.001, ANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=�
-łŃ ġĴİĤĚpÿĊč«ĀR«�eĻ{9ŋ|«¬�pļMT8148 gÿġĴİĤĚpĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷ŀġĴİĤĚpw�H ÛÑ�û«Ā�&ā 50 % �ąû��ô÷ĀøÕѤĉïÿx?ô 1.5 _Äû«Ā�&ā 5 % �ąûx?ô÷ŀĻ *P�0.05, **P�0.01, ANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
�
�
�
(%)
�
� �� � �� �
�
�����
����������
���
* *
**
**
*
*-,+()#%'�$ ���/��1,.Q0�
100
40
20
(%)
0
80
60
0� 1� 100�
MT8148�
��(ppm)�
����������� **�
*�
*-,+()���"�� !�������& /Student t ���*P<0.05, **P<0.010�
***�
10�
-łń ġĴİĤĚpÿĊč«ĀR«�eĻ{9ŋ|«¬�pļMT8148 gÿ%�}EĀġĴİĤĚpĐw�H 1.5 _ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷ŀġĴİĤĚp}E 10 ppm �ÿîìú«Ā�&ā`Mÿx?ôľ100 ppm ûā10 % �ąûx?ô÷ŀĻ*P�0.05, **P�0.01, ANOVA ĀH post-hoc ´düôú Bonferroni
rÿĊČh=ļ
-łŅ ġĴİĤĚpÿĊč«ĀR«�eĻ{9ŋ|«¬�pļ6715 gÿġĴİĤĚpĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷ŀġĴİĤĚpw�H 5 �û«Ā�&ā 60 % �ąûx?ôľ3 _ÄHÿāÕÑ㥠0 % ąûx?ô÷ŀĻ **P�0.01,***P�0.001, ANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
���
��
��
� �
�
��
� � �� �� �� ��
��
�����
����������
���
���
**
******
*** ***
***
(%)
-łņ ġĴİĤĚpÿĊč«Ā4mP��eĻ{9ŋBrain Heart Infusion ÓBHIÔÑu 0.ļMT8148 gÿ 100 ppm ĀġĴİĤĚ�aĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷üòďľ�úĀ_Äÿîìú`M@ā·ćċĎþïù÷ŀ
*-,+()#%'�$ ���/��1�HI0�
100
40
20
(%)
0
80
60
3� 6� 12�
MT8148�
���$���
�����������
120
100
40
20
(%)
0
80
60
3� 6� 12�
SA31�
���$���
�����������
120
*-,+()#%'�$ ���/��1TH0�
100
40
20
(%)
0
80
60
3� 6� 12�
MT8148�
*-,+()���$���
���� ������������
120
100
40
20
(%)
0
80
60
3� 6� 12�
SA31�
*-,+()���$���
���� ������������
120
-łŇ ġĴİĤĚpÿĊč«Ā4mP��eĻ{9ŋBHI u 0.ļSA31 gÿ 100 ppm ĀġĴİĤĚ�aĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷üòďľ�úĀ_Äÿîìú`M@ā·ćċĎþïù÷ŀ
,/.-*+%')�&����1��3�HI2�
100
40
20
(%)
0
80
60
�� ��
MT8148�
��&���
�����������
120
100
40
20
(%)
0
80
60
�� ��
SA31�
��&���
�����������
120
,/.-*+%')�&����1��3TH2�
100
40
20
(%)
0
80
60
�� ��
MT8148�
��&���
�����������
120
100
40
20
(%)
0
80
60
�� ��
SA31�
��&���
�����������
120
��� ���
��� ���
Êw�ÿ>õč«ZĀ�&
-łň ġĴİĤĚpÿĊč«Ā4mP��eĻ{9ŋTodd Hewitt ÓTHÔÑu 0.ļMT8148 gÿ 100 ppm ĀġĴİĤĚ�aĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷üòďľ�úĀ_Äÿîìú`M@ā·ćċĎþïù÷ŀ
,/.-*+%')�&����1��3�HI2�
100
40
20
(%)
0
80
60
�� ��
MT8148�
��&���
�����������
120
100
40
20
(%)
0
80
60
�� ��
SA31�
��&���
�����������
120
,/.-*+%')�&����1��3TH2�
100
40
20
(%)
0
80
60
�� ��
MT8148�
��&���
�����������
120
100
40
20
(%)
0
80
60
�� ��
SA31�
��&���
�����������
120
��� ���
��� ���
Êw�ÿ>õč«ZĀ�&
-łʼn ġĴİĤĚpÿĊč«Ā4mP��eĻ{9ŋTH u 0.ļSA31 gÿ 100 ppm ĀġĴİĤĚ�aĐw�HĀ%_ÄÿîñčÊw�ÿ>õč«ZĀ�&Đqć÷üòďľ�úĀ_Äÿîìú`M@ā·ćċĎþïù÷ŀ
,/.-*+%')�&����1��3�HI2�
100
40
20
(%)
0
80
60
�� ��
MT8148�
��&���
�����������
120
100
40
20
(%)
0
80
60
�� ��
SA31�
��&���
�����������
120
,/.-*+%')�&����1��3TH2�
100
40
20
(%)
0
80
60
�� ��
MT8148�
��&���
�����������
120
100
40
20
(%)
0
80
60
�� ��
SA31�
��&���
�����������
120
��� ���
��� ���
Êw�ÿ>õč«ZĀ�&
-łŊ ġĴİĤĚpÿĊčĠĚĸĺĠ�<L��§ÿ>õčP��eMT8148 gĐ�ì÷2&ľ¼AĀ BHI }EÿîìúāġĴİĤĚpw�üÊw�ÿîñč���Ā@ā·ćċĎþïù÷ĈĀĀľġĴİĤĚĐw�õčòüÿĊȶÎ�5ÿ��õč£ĭĴĺĚÁāx?ô÷ŀ
����� �
�������
0
�%�
���
20
40
60
80
100
-ŃŁ ġĴİĤĚpÿĊčĠĚĸĺĠ�<L��§ÿ>õčP��eMT8148 gĐ�ì÷2&ľ BHI Ā}EĐ 1/10ÿõčüľġĴİĤĚpĐw�õčòüÿĊČ���Ā`Mþ��Đ·ć÷.Ó***P�0.001ÕÑANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
***
����� �
�������
0
�%�
���
20
40
60
80
100
-Ńł ġĴİĤĚpÿĊčĠĚĸĺĠ�<L��§ÿ>õčP��e6715 gĐ�ì÷2&ľ¼AĀ BHI }EÿîìúľġĴİĤĚpĐw�õčòüÿĊČ���Ā`Mþ��Đ·ć÷.Ó*P�0.05 Õ ANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
���
��
��
�
�
�
*�%�
��� �����
��� �����
���
-ŃŃ ġĴİĤĚpÿĊčĠĚĸĺĠ�<L��§ÿ>õčP��e6715 gĐ�ì÷2&, BHI Ā}EĐ 1/10 ÿõčüľġĴİĤĚpĐw�õčòüÿĊČ���Ā`Mþ��Đ·ć÷.Ó*P�0.05ÕÑANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
��
��
�
��
*
������
���
������
�%�
B
A
������ (-) ������ (+)
������ (-) ������ (+)
¼AĀ BHI}EľġĴİĤĚpĻĿļ ¼AĀ BHI }EľġĴİĤĚpĻĽļ
B
A
������ (-) ������ (+)
������ (-) ������ (+)
-Ńń ���ķĺĝĺËJÃÿĊčĩēėĬĒĶıĀ´dMT8148 gĐ�ì÷2&ľ¼AĀ BHI }EîĊĂ 1/10 Ā}EĀ BHI ÿîìúľġĴİĤĚpĐw�õčòüÿĊČĩēėĬĒĶıGOÁĀ��Đ·ć÷ŀ
1/10 Ā BHI}EľġĴİĤĚpĻĿļ 1/10 Ā BHI }EľġĴİĤĚpĻĽļB
A
������ (-) ������ (+)
������ (-) ������ (+)
B
A
������ (-) ������ (+)
������ (-) ������ (+)
-ŃŅ MT8148 gĀĩēėĬĒĶıGOÁġĴİĤĚpĐw�õčòüÿĊČĩēėĬĒĶıGOÁĀ`Mþ��Đ·ć÷ŀÓ***P�0.001ÕÑANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
���
���
���
�
���
OD595�
���� ��
���� ��
***
-Ńņ 6715 gĀĩēėĬĒĶıGOÁġĴİĤĚpĐw�õčòüÿĊČĩēėĬĒĶıGOÁĀ`Mþ��Đ·ć÷ŀÓ***P�0.001ÕÑANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
��
���
���
�
���
���
***
�� �����
�� �����
OD595�
-ŃŇ íðì�HĀ$¨���Ā�±�
İĨĴĶĔĖĺĢĺûĀíðì� İĨĴĶĔĖĺĢĺûĀíðìH
ġĴİĤĚpûĀíðì� ġĴİĤĚpûĀíðìH
-Ńň İĨĴĶĔĖĺĢĺĐ��ô÷íðì�HĀĭĴĺĚĠĜđİĨĴĶĔĖĺĢĺĐ��ôú 1 ¾ÄíðìĐ¯ùúĈĭĴĺĚĠĜđÿ`M@ā·ćċĎþïù÷ŀ
0
50
100
150
�����
���� ����
-Ńʼn ġĴİĤĚpĐ��ô÷íðì�HĀĭĴĺĚĠĜđġĴİĤĚpĐ��ôú 1 ¾ÄíðìĐ¯íòüÿĊČľĭĴĺĚĠĜđā`Mÿx?ô÷ŀĻ***P�0.001, ANOVA ĀH post-hoc ´düôú Bonferroni rÿĊČh=ļ
0
50
100
150
���� ����
�����
***
-ŃŊ íðì�HûĀ+u� S. mutansĀ4��íðì�Ā+u�Ā S. mutans «ZĐ Ø üô÷ÈĀíðìHĀ S. mutans «ZĀ4��Đqć÷ŀİĨĴĶĔĖĺĢĺûíðìHĀ S. mutans «ZĀ4��Đ100 % üôú��ôľġĴİĤĚpûĀíðìHüoºô÷ŀġĴİĤĚpûíðìĐô÷HĀ+u� S. mutans «ZāİĨĴĶĔĖĺĢĺûíðìĐô÷Hüoºô40 %�ąû`Mÿx?ô÷ŀĻ*P�0.05, ANOVAĀH post-hoc ´düôú BonferronirÿĊČh=ļ
*
� ��� �� �����
���
�%�
20
40
60
80
100
120
140
0
Top Related