Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4...

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Title セラミック水の Streptococcus mutans におけるう蝕 原性抑制効果の検討 Author(s) 米山, 莉紗 Citation Issue Date Text Version ETD URL https://doi.org/10.18910/72249 DOI 10.18910/72249 rights Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Note Osaka University Knowledge Archive : OUKA Osaka University Knowledge Archive : OUKA https://ir.library.osaka-u.ac.jp/repo/ouka/all/

Transcript of Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4...

Page 1: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

Title セラミック水の Streptococcus mutans におけるう蝕原性抑制効果の検討

Author(s) 米山, 莉紗

Citation

Issue Date

Text Version ETD

URL https://doi.org/10.18910/72249

DOI 10.18910/72249

rights

Copyright: All rights reserved. No part of thispublication may be translated into otherlanguages, reproduced or utilized in any form orby any means, electronic or mechanical,including photocopying, recording, microcopying,or by any information storage and retrievalsystem, without permission in writing from thepublisher.

Note

Osaka University Knowledge Archive : OUKAOsaka University Knowledge Archive : OUKA

https://ir.library.osaka-u.ac.jp/repo/ouka/all/

Page 2: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

Osaka University

Page 3: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

Streptococcus mutans

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1

6074-

-

Streptococcus mutans

Hamada Slade, 1980 S. mutans

, 1997 S. mutans

190 kDa Protein

antigen PA Okahashi , 1989

S. mutans 3 Glucosyltransferase ; GTF GTFB

GTFCGTFD

Ooshima , 2001 S. mutans

Ooshima , 2001 S. mutans

Glucan-binding protein ; Gbp GTF

S. mutans Banas

, 2003

S. mutans

Hamada Slade, 1980Hamada , 1984

Cnm Cbm

CBP S. mutans

Nomura , 2012

Smith , 2002

Matsumoto , 1999Signoretto , 2010

Smith , 2002

Gluzman , 2013

Page 5: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

2

He , 2015

β-TCP

Myoui , 2006

3,000

, 2016

S. mutans

in vitro S. mutans

-

-

-

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3

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S. mutans

8

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S. mutans MT8148

c CBP PA Ooshima 1983

SA31 k Cbm PA Nakano 2008

Streptococcus sobrinus 6715 g Ooshima 1993

Brain Heart Infusion BHI; Difco Laboratories Detroit, MO, USA

Todd Hewitt TH; Difco Laboratories Mitis Salivarius MS

Difco Laboratories, Detroit, MO, USA 0.2 U/mL; Sigma-Aldrich

Co., St. Louis, MO, USA 15 % w/v MSB

BHI TH 37 18

MSB 95 % 5% 37 48

Page 7: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

4

NK �%��4,18��

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Page 8: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

5

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3

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3,000 rpm 10 10 mM Hexidium Iodide

Molecular Probes, Eugene, OR, USA 5 µL 1 mL

15 100 ppm

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60 30 12,000 rpm 1

25 %

Chambered Coverglass System CultureWell™,Grace Bio Lab, Bend, OR, USA 150

µL 37 2

200 µL 37

24 PBS 100 µL

488 nm

TSC-SP5 Leica Microsystems GmbH, Wetzlar, Germany

DMI6000B Leica 63×

Image J version 1.34s National Institutes of Health, Bethesda, MD, USA

Ardin 2014 10

mL BHI 37 18 MT8148 6715

3,000 rpm 10 100 ppm

0.25 % BHI OD600 0.1

Page 9: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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Quiugley Hein

1962 Red-

Cote, John O.Butler Co., Chicago, IL, USA

0 3 4

2

1 10

Page 10: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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5 %

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11

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19

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P

Page 12: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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0.001 20 6715 BHI

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-

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-

Page 13: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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"-

-

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64:36 1 0.08 ppm

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Lu , 2003

Yao , 2008

Burne

, 1987

Burne , 1987Bellantone , 2002Hipler ,

2006 S. mutans

Burne , 1987

Castillo ,

2011

Kawasita , 2000

H2O2 UV

Porphyromonas gingivalis Fusobacterium nucleatum S. mutans

Feuerstein , 2006Ikai , 2013

Page 14: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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MT8148 SA 31 MT8148

S. mutans

c/e/f/k

Hamada , 1980Nakano , 2004 S. sobrinus

d/g Okahashi , 1986 MT8148

SA31 c k

MT8148

SA31

Nomura , 2012 SA 31 MT8148

S. mutans

SA31 PBS

PBS

0.1 ppm

NaCl

5.0 wt % 1 ppm

Cl- AgCl

NaCl

10 SA31

PBS

, 2016

PBS

BHI

S. mutans S. sobrinus

Page 15: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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S. mutans S. sobrinus

BHI

BHI

BHI

S. mutans

99.5 %

0.5 % Na+ K+

Ca2+ Cl-HCO3- Hofman

, 2001Kaufman , 2002

S. mutans

Smith , 2002

Ooshima , 1994

S. mutans S. mutans

S.

mutans Ooshima , 1994

S. mutans

S. mutans

Hamada , 1984

Moriwaki , 2011

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13

TiC

BHI

S. mutans

S. mutans

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(-

PBS

S. mutans

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S. mutans

S. mutans

Page 18: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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)+-

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Page 22: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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Page 23: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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Page 24: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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Page 25: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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Page 26: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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Page 27: Osaka University Knowledge Archive : OUKA...1 6074-- Streptococcus mutans 'Hrm ~ H W H H&uxK Í %! 4 é %& " ä36 5 Hamada " Slade, 1980 S. mutans ' &Ô uxK = %24 @36 @^tFz9 Ñ Ï

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