8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
1/11
Spider silk proteinsBasis for new materials
Thomas Scheibel
Lehrstuhl Biomaterialien
Universitt Bayreuth
www.fiberlab.de
Outstanding properties of spider silks are well known for thousands of years
high tensile strength
high elasticity
no inflammation
no allergic response
Spider silk a protein fiber
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
2/11
Spider silk a diverse biopolymer
Rmer and Scheibel, Chem. Unserer Zeit2007, 41, 306Vendrely and Scheibel, Macromol. Biosci. 2007, 7, 401Heim et al.,Angew. Chem. Int. ed. 2009, 48, 3584
Spider silk fibers: mechanical properties
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
3/11
- vascular wound repair devices
- haemostatic dressings and sutures
- drug delivery systems
- cosmetical products
- technical textiles / protective clothing
- surface / fiber coatings
- etc., etc., etc.
Possible applications for silk-based materials
availabilityofthenaturalproteins(e.g.cannibalismofspiders)
organizationofgenes
solution
analysisofproteincomponents
reversetranslationandgene designformodernbiotechnology
problems
Vendrely and Scheibel, Macromol. Biosci. 2007, 7, 401
Employing silk proteins for analysis
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
4/11
Processing technology
Huemmerich et al., Biochemistry2004, 43, 13604Vendrely and Scheibel, Macromol. Biosci. 2007, 7, 401
can be biotechno-logically mimicked
Natural processing of spider silk proteins
Heim et al.,Angew. Chem. Int. ed. 2009, 48, 3584
What Spiderman should know
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
5/11
Analysis of laminar flow and shear forces
Silk assembly in designed microfluidic device
10 m
simultaneous phase separation, pH-change and elongational flow
mixing andlaminar flow
analysis
Rammensee et a., PNAS 2008, 105, 6590
SEM
Possibleapplicationsintechnicaltextiles,
vascularwoundrepairdevices,andsutures.
Biomimetic spinning of spider silk proteins
Rmer and Scheibel, Bionik: Patente aus der Natur,2007, 130
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
6/11
from nature
to new materials
Hardy et al., Polymer2008, 49, 4309
Advanced processing of spider silk proteins
Spie et al., GIT2007, 11, 928
strengthO2 permeability
cm1m/m/d (23C)
H2O permeability
85% humidity
g1m/m/d (23C)
silk film 20 MPa 18500 17500
PE 24 MPa 55000 30
PP 33 MPa 70000 55
PET 74 MPa1400
200
Films made of biotech silk
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
7/11
Microcapsules made of biotech silk
emulsion in toluene
Hermannson et al,Adv. Mater. 2007, 19, 1810Hermannson et al, Phys. Chem. Chem. Phys. 2007, 9, 6442
Microcapsules made of biotech silk
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
8/11
10 m
emulsion in toluene transfer into water
Microcapsules made of biotech silk
Hermannson et al,Adv. Mater. 2007, 19, 1810Hermannson et al, Phys. Chem. Chem. Phys. 2007, 9, 6442
Release is triggered by the addition of Proteinase K
Controlled release of encapsulate
light microscopy
fluorescencemicroscopy
Hermannson et al,Adv. Mater. 2007, 19, 1810
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
9/11
Microspheres made of biotech silk
Lammel et al, ChemSusChem. 2008, 1, 413Slotta et al,Angew. Chem. Int. Ed. 2008, 47, 4592
Spider silk microspheres can be used for protein and drugencapsulation.
Spider silk microsphere formation
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
10/11
Zeit[min]
drugrelease
Drug encapsulation in silk microspheres
Spidersilkmicrospherescanbeusedforencapsulatingpoorlywatersolublesubstances.
Liebmann et al., Colloids and Surfaces A: Physicochem. Eng. Aspects, 2008, 331, 126
E-carotene release in intestinalfluids
noE-carotene release in gastric fluids
in collaboration with
former Lab membersChristian Ackerschott
Simone HessDaniel Hmmerich
Lin RmerSayam SenGupta
Charlotte Vendrely
TUMHorst Kessler
Andreas Bausch
LMUGerhard Winter
Martin-Luther-Universitt HalleRainer Rudolph
Universitt Sussex, UKLouise Serpell
BayerischesStaatsministeriumfr
Umwelt,GesundheitundVerbraucherschutz
and further supporting companies
Acknowledgement
8/8/2019 Innoday09 Pdf11 Uni-bayreuth Tscheibel
11/11
Fibrous proteins for new applications
Top Related