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fB Y T

TRNG I HC DC H NI

PHAN TH AN

NGHIN CU S THAY I

HM LNG V THNH PHN

TINH DU I BI THEO THI GIAN

KHA LUN TT NGHIP DC S

H NI-2013

B Y T

TRNG I HC DC H NI

PHAN TH AN

NGHIN CU S THAY I

HM LNG V THNH PHN

TINH DU I BI THEO THI GIAN

KHA LUN TT NGHIP DC S

Ngi hng dn:

DS. Nghim c Trng

Ni thc hin:

1. B mn Thc vt Trng i hc

Dc H Ni

2. X Yn Ninh huyn Ph Lng tnh

Thi Nguyn

H NI-2013

LI CM N

Kha lun ny c thc hin ti B mn Thc vt Trng i hc

Dc H Ni v x Yn Ninh - huyn Ph Lng tnh Thi Nguyn.

Vi lng knh trng v bit n su sc, ti xin by t li cm n chn

thnh ti DS. Nghim c Trng, ngi thy trc tip hng dn, tn tnh

ch bo, ng vin v to iu kin tt nht cho ti trong qu trnh hon thnh

kha lun tt nghip ny.

Ti cng xin by t li cm n chn thnh ti:

PGS.TS. Trn Vn n, ngi thy hng dn, ch bo tn tnh v

ng gp nhng kin qu bu cho ti trong qu trnh thc hin nghin cu

khoa hc.

Cng cc ging vin B mn Thc vt: TS. Hong Qunh Hoa, TS.

Nguyn Quc Huy, ThS. V Vn Anh, ThS. Phm H Thanh Tng v tt

c cc ch k thut vin B mn Thc vt lun sn sng ch bo v gip ti

hon thnh nghin cu ny.

Anh Trn Vit Vn Cng ty C phn DKNatura, em on Th

Phng Tho A4K65, bn T Khc Cng M1K63 gip ti trong

qu trnh ly mu.

Ti cng xin gi li cm n chn thnh ti cc thy c gio trng i

hc Dc H Ni nhng ngi thy du dt ti sut 5 nm hc va qua.

Cui cng, ti xin chn thnh cm n gia nh v bn b lun bn

cnh, ng vin v gip ti trong qu trnh hon thnh kha lun.

H Ni, ngy 20 thng 5 nm 2013

Sinh vin

Phan Th An

MC LC

Danh mc k hiu, cc ch vit tt

Danh mc cc bng

Danh mc cc hnh Trang

T VN ...1

PHN 1: TNG QUAN...3

1.1. Loi i bi Blumea balsamifera (L.) DC................................................3

1.1.1. V tr phn loi loi Blumea balsamifera (L.) DC..3

1.1.2. c im thc vt...3

1.1.3. Phn b, thu hi v ch bin...4

1.1.4. Nhng nghin cu v tinh du i bi.....4

1.2. Borneol..7

1.2.1. Cng thc phn t...7

1.2.2. Ngun gc, iu ch....8

1.2.3. Tc dng, cng dng.12

CHNG 2: I TNG V PHNG PHP NGHIN CU..15

2.1. Nguyn vt liu, thit b.15

2.1.1. Nguyn liu nghin cu15

2.1.2. Thit b v ha cht nghin cu16

2.2. Ni dung nghin cu..............................................................................16

2.2.1. Nghin cu c im thc vt.......16

2.2.2. Nghin cu hm lng Borneol16

2.3. Phng php thc nghim17

2.3.1. Nghin cu c im thc vt.......17

2.3.2. Nghin cu hm lng Borneol17

CHNG 3: THC NGHIM, KT QU V BN LUN...19

3.1. Kt qu thc nghim..19

3.1.1. c im thc vt v hm lng Borneol ca cc mu i bi.... 19

3.1.2. S thay i hm lng Mai hoa bng phin theo thi gian..30

3.2. Bn lun...32

KT LUN V KIN NGH.34

Kt lun......34

Kin ngh....35

TI LIU THAM KHO

PH LC

Ph lc 1: Phiu gim nh tn khoa hc

Ph lc 2: Giy chng nhn m s tiu bn

Ph lc 3: Pic sc k GC-MS ca MHBP cc mu nghin cu

DANH MC K HIU, CC CH VIT TT

GABA Gamma amino butyric acid

GC-MS Sc k kh khi ph

(Gas chromatography-mass spectrometry)

IC50 Nng c ch 50% i tng th

(Inhibitory Concentration)

MHBP Mai hoa bng phin

MS Khi ph (Mass spectrometry)

PTMD Phn trn mt t

TD Tinh du

TLTK Ti liu tham kho

TT Th t

DANH MC CC BNG

TT Tn bng Trang

1 Bng 1.1: Thnh phn tinh du i bi thu hi ti H

Giang, H Ni, c Lc

5

2 Bng 1.2: Hm lng v thnh phn tinh du i bi thu

hi ti mt thi im

6

8 Bng 1.3: Thnh phn tinh du i bi Bangladesh 7

3 Bng 1.4: Ngun thc vt cha tinh du c Borneol 9,10,11

4 Bng 2.1. Danh sch mu i bi 15

5 Bng 3.1: Cc c im hnh thi khc nhau gia cc

mu

23

6 Bng 3.2: T l Borneol trong Mai hoa bng phin (thng

11)

29

7 Bng 3.3: Hm lng Borneol trong cc mu (thng11) 29

9 Bng 3.4: Hm lng Mai hoa bng phin trong cc mu

nghin cu

31

DANH MC CC HNH

TT Tn hnh Trang

1 Hnh 1.1: Cng thc cu to ca Borneol 8

2 Hnh 1.2: S tng hp Borneol t Camphor 12

3 Hnh 3.1: c im hnh thi c quan sinh dng 20

16 Hnh 3.2: c im hnh thi c quan sinh sn (1) 21

4 Hnh3.3: c im hnh thi c quan sinh sn (2) 22

5 Hnh 3.4: S khc nhau v mu sc thn gn im phn

cnh

24

6 Hnh 3.5: S thay i hnh thi l cc giai on 24

7 Hnh 3.6: So snh mt lng che ch 24

8 Hnh 3.7: c im ngn l bc 25

9 Hnh 3.8: c im mu sc u trng hoa lng tnh 25

10 Hnh 3.9: c im vi phu thn. 26

11 Hnh 3.10: c im vi phu gn l 27

12 Hnh 3.11: c im vi phu phin l 28

13 Hnh 3.12: Biu hm lng Borneol trong dc liu

kh tuyt i ca cc mu (thng 11)

30

14 Hnh 3.13: Biu s thay i hm lng Mai hoa bng

phin 8 mu i bi theo thi gian

32

1

T VN

Borneol l mt dn cht cha oxy ca monoterpen. Borneol c s

dng trong Y hc c truyn ca nhiu nc trn th gii nh Trung Quc,

Vit Nam, n , Philippines,Trong Y hc c truyn Trung Quc v Vit

nam, Borneol c dng vi tn gi Mai hoa bng phin, c 2 cng nng l:

(i) tiu tn mng mng v (ii) khai khiu tnh thn [9]. Hin nay, Borneol l

mt trong nhng nguyn liu c gi tr cao trong ngnh dc.Theo Dc in

Trung Quc, Borneol l thnh phn quan trng ca khong 63 sn phm t

tho dc [31]. Vit Nam, Borneol c trong thnh phn ca nhiu Dc

phm thuc cc nhm thuc khc nhau nh nhm gim au - khng vim (cao

dn Sinsinpa), nhm thuc tim mch (PC.CARDIO Vin h tm) v cc sn

phm thuc nh mt (Osla, Eyelight Cool, Vimaxx, Optamix,). Hin nay,

trn th trng ha cht c hai loi Borneol khc nhau: (i) Borneol tng hp l

hn hp ca DL-Borneol v Isoborneol (c c tnh) [4]; (ii) Borneol t nhin

c thnh phn chnh l D-Borneol. Theo mt s nghin cu, Borneol tng hp

b bin tnh, phn hy trong qu trnh bo qun v bin i thnh Camphor

(c th ti 45%-97%) l thnh phn c c tnh; trong khi Borneol t nhin

khng c c tnh [16], [30]. V vy, ngy nay ngi ta c xu hng s dng

Borneol t nhin thay v s dng Borneol tng hp.Ngun Borneol thin

nhin ch yu c ly t cc cy thuc h Dipterocarpaceae (Dryobalanops

aromatic C. F. Gaeth), h Asteraceae (Blumea balsamifera (L.)DC.). Hin

nay ngun nguyn liu sn xut Borneol thin nhin ang bi thiu ht, gi ca

Borneol trn th trng tng ln. Do , vic to ngun nguyn liu sn xut

Borneol thin nhin l rt cn thit.

Vit Nam, t thi chng Php, nhn dn ta bit khai thc Borneol

t cy i bi [7].Cy i bi c c tnh qu l d trng, d chm sc, c th

2

pht trin nhiu ni, trn cc vng t kh hn [24]. T l Borneol v

Camphor trong tinh du i bi thay i theo tng vng: H Giang: 57,82%

Borneol, 1,12% Camphor; H Ni: 50,57% Borneol, 18,71% Camphor; c

Nng: 5,70% Borneol v 70,05% Camphor [5], [11]. Vi cng mt thi im

thu hi, cc mu i bi thu hi ti cc a im khc nhau c hm lng

Borneol trong dc liu kh tuyt i rt thay i: H Ni: 0,03%; Thi

Nguyn: 0,01%; H Giang: 1,75%; Nam nh: 0,26% [8]. khai thc

Borneol t cy i bi t hiu qu cao cn phi nghin cu tm ra thi im

thu hi i bi thch hp, ging i bi v vng trng i bi cho hm lng

Borneol cao.

Vi cc l do trn, bc u nhm to c ngun nguyn liu sn xut

Borneol t nhin t cy i bi v tm ra thi gian thu hi i bi thch hp,

chng ti thc hin ti: Nghin cu s thay i hm lng v thnh

phn tinh du i bi theo thi gian vi cc mc tiu:

1. nh gi s khc nhau v c im thc vt v hm lng Borneol ca

cc mu trong cng mt iu kin sinh thi.

2. Nghin cu s thay i hm lng Mai hoa bng phin ca cc mu

i bi theo thi gian.

3

CHNG 1: TNG QUAN

1.1. Loi i bi Blumea balsamifera (L.) DC.

1.1.1. V tr phn loi loi Blumea balsamifera (L.) DC.

Chi Blumea DC. c khong 55 loi trn th gii, phn b chu Phi,

ng Nam ti Bc chu i Dng. Vit Nam c 30 loi, gp ph bin t

ng bng, trung du ti min ni [2].

Loi Blumea balsamifera (L.) DC. thuc phn lp cc: Asteridae; Lin b

Cc: Asteranae; B cc: Asterales; H cc: Asteraceae; Chi: Blumea.

Tn ng ngha: Conyza balsamifera L.; Baccharis salviaLour.;Blumea

balsamifera var. microcephala Kitam.; Pluchea balsamifera (L.) Less [2], [3].

1.1.2. c im thc vt

Theo cc ti liu [2], [3], [6], [38] loi Blumea balsamifera (L.) DC. c

cc c im:

Cy bi hoc cy bi thp, cy lu nm, cao 1-3 m. Thn phn cnh

dng ng pha ngn, nhiu lng, gc cnh ha g nhiu, v mu nu xm.

Cnh dng ng thng, dy lng mu trng hi vng. L mc so le, thun di,

kch thc 15-18 x 3,5-5 cm; hp gc l; mt di l ph nhiu lng mt,

lng che ch a bo nm thng so vi mt l; cung l ngn, mang tai l di

10-12 mm; mp l x rng ca tha cho n dy, rng cong ln, nh l nhn;

10-12 cp gn.

Cm hoa u 6-7 mm, c cung, hnh chy; l bc gm 3-4 vng

ngoi, mt ngoi ph nhiu lng p st vo nhau, l bc ngoi nh hn, trn

nhn, di 1-3 mm, l bc trong di hn, hnh di hp di 5-6 mm; ng knh

cm hoa 2,5-3 mm, c l t ong, ph lng; hoa ngoi hnh chy di n 6

mm, x 2-4 thy; hoa gia hnh ng, mu vng, khng cung, di 6-7 mm,

thy c gai tht nh, tha tht lng a bo.

4

Qu b, mu nu, thun di khong 1 mm; ph lng tha hoc dy; trn

c mo lng hi , di 4-6 mm.

1.1.3. Phn b, thu hi v ch bin

Phn b

i bi mc hoang nhiu Vit Nam, Trung Quc v cc nc nhit i

Nam nh n , Indonesia, Philippines,

Vit Nam, i bi phn b rng, hu ht cc tnh min ni thp di

1000m, trung du, ng bng v nhiu o ln, khng c trong rng su. Cy

d trng, a sng, sng 1-2 nm, c th nhn ging bng ht, cnh hoc cy

con [1], [6], [24].

Thu hi

L i bi c th thu hi 4 t trong 1 nm vi sn lng 50 tn/ha, cho

khong 50-200 kg Borneol [24]. Tinh du l i bi c khai thc t lu i

vi tn thng phm l Mai hoa bng phin. Nhn dn ta s dng Mai hoa

bng phin t th k X. T thi khng chin chng Php, nhn dn ta bit

khai thc Borneol t i bi, sau tm dng. Ma thu ng ct Mai hoa bng

phin cho hm lng Bng phin cao nht. Tinh ch Bng phin bng cch p

bt tinh du, trn Bng phin th vi bt than ci vi t l vi bt: than ci:

Bng phin l 3:5:100; cho hn hp thng hoa, thu ly Bng phin tinh ch

[6].

1.1.4. Nhng nghin cu v tinh du i bi

Nhng nghin cu trong nc

- Trong l i bi thng cha 0,21,8% tinh du vi thnh phn ch

yu l D-Borneol, L-Camphor, Cineol, Limonen, acid Palmitic, acid Myristic,

cn cha cc Sesquiterpen alcol [1], [5].

5

- Tinh du l i bi c thu thp 3 tnh (H Giang, H Ni, c Lc)

c cc thnh phn chnh c trnh by bng 1.1, vi t l Borneol rt thay

i t 5,70% - 57,82% (Bng1.1) [11].

Bng 1.1: Thnh phn tinh du i bi thu hi ti

H Giang, H Ni, c Lc

a im

Thnh phn chnh

H Giang

H Ni c Lc

Borneol 57,82% 50,57% 5,70%

Camphor 1,12% 18,71% 70,05%

-caryophyllen 8,27% 10,06% 10,54%

-cadinol 7,95% 3,14% -

Caryophyllen oxid 3,10% - -

Patchoulen - 2,99% -

Veridiflorol - 2,01% -

Carvacrol - - 5,70%

- Vi cng mt thi im thu hi, cc mu i bi thu hi ti cc a

im khc nhau c hm lng tinh du trong dc liu kh tuyt i, hm

lng Borneol v hm lng Camphor trong tinh du rt thay i (Bng 1.2)

[8].

6

Bng 1.2: Hm lng v thnh phn tinh du i bi

ti mt thi im thu hi

a im HLTD trong DL

kh tuyt i

HL Borneol trong

tinh du

HL Camphor

trong tinh du

i hc Dc H

Ni, H Ni

0,32% 8,83% 52,38%

Cm Lnh, Ba V,

H Ni

0,97% 82,23% 1,89%

Yn Ninh, Ph

Lng, Thi

Nguyn

0,36% 3,34% 80,63%

V Xuyn, H

Giang

2,19% 79,85% 0,72%

Xun Trng,

Nam nh

1,00% 25,54% 56,43%

Nhng nghin cu trn th gii

L i bi Myanmar cha 1-9% tinh du trong 75% Camphor v 25%

Borneol. Tinh du i bi Philippines c hm lng t 0,1-0,4%. Thi

Lan, trong tinh du, Borneol chim t l 25%, Camphor chim 75%. Tinh du

i bi Trung Quc thnh phn ch yu l Borneol [1].

Tinh du l ti i bi ti Bangladesh c hm lng l 0,40%; xc nh

c 50 thnh phn, trong thnh phn chnh l Borneol (Bng 1.3) [26].

7

Bng 1.3: Thnh phn tinh du i bi Bangladesh

1.2. Borneol

1.2.1. Cng thc phn t

Borneol l dn cht monoterpen 2 vng cha oxy, cng thc phn t:

C10H18O (Hnh 1.1).

TT Thnh phn T l (%)

1 -pinene 0,48

2 -pinene 1,2

3 D-limonene 0,2

4 Borneol 33,2

5 Camphor 0,1

6 Linalool 1,3

7 Caryophyllene 8,2

8 Ledol 7,1

9 Phytol 4,6

10 Caryophyllene oxide 4,1

11 -eudesmol 3,2

12 Guaiol 3,4

13 Thujopsene 4,4

8

Hnh 1.1: Cng thc cu to ca Borneol

- Tn IUPAC: endo-1,7,7-Trimethyl bicyclo [2.2.1] heptan-2-ol.

- Tn ng ngha: endo-2-bornanol; endo-2-camphanol; endo-2-

hydroxycamphane; Bornyl alcohol; Borneo camphor; Dryobalanops camphor;

Camphol [21], bng phin, ngi phin, long no hng, i ngi [7].

- Khi lng phn t: 154,25 g/mol. T trng: 1,011 g/cm3 20C. D

thng hoa nhit thng, kh nng thng hoa km hn Camphor; tinh th

khng mu, mi thm c bit v d chu.

- Nhit si: 206C-212C. Nhit nng chy 204C-208C.

- tan: Khng tan trong nc, tan trong alcol: 176:100, ether (1:6);

Benzene (1:5); Toluen; Aceton [21]. Cho phn ng mu vi dung dch

Vanilin sulfuric hay Vanilin clohydric [7].

1.2.2. Ngun gc, iu ch

iu ch t thin nhin

Borneol c th ct cng nghip t: l cy i bi Blumea balsamifera

(L.) DC. (Asteraceae), qu cy Sa nhn Amomum villosum var. xanthioides

(Wall. ex Baker) T.L.Wu & S.J.Chen (Zingiberaceae), cy Long no hng

Dryobalanops aromatic C. F. Gaertn. (Dipterocarpaceae), cy Cinnamomum

burmannii (Ness. & T.Ness) Blume (Lauraceae) [6], [7], [15]. Hn na

Borneol cn l thnh phn trong tinh du ca nhiu cy khc (Bng 3.4).

9

Bng 1.4: Ngun thc vt cha tinh du c Borneol

TT

Tn khoa hc

H

Tn

Vit

Nam

B

phn

khai

thc

Hm

lng

TD

(%)

T l

Borneol

(%)

TL

TK

1 Achillea distans Waldst. & Kit. ex

Willd.

Asteraceae L 7,5 [13]

2 Amomum villosum var. xanthioides

(Wall. ex Baker) T.L.Wu & S.J.Chen

Zingiberaceae Sa nhn Ht 2-3 19 [6]

3 Artemisia afra Jacq. ex Willd Asteraceae L 1,5 5,1 [19]

4 Blumea martiniana Vaniot Asteraceae i bi l

ln, Kim

u

L 6,24 [25]

5 Dipterocarpus turbinatus C. F. Gaernt. Dipterocarpaceae Thn 30-65 [34]

6 Cinnamomum burmanii (Nees &

T.Nees) Blume

Lauraceae L 0,60 78,6 [15]

7 Kaempferia galanga L. Zingiberaceae a lin R 2,4-3,9 2,7 [6]

10

TT

Tn khoa hc

H

Tn

Vit

Nam

B

phn

khai

thc

Hm

lng

TD

(%)

T l

Borneol

(%)

TL

TK

8 Micromeria cristata (Hampe) Griseb.

subsp. phrygia P. H. Davis

Lamiaceae L 27-39 [34]

9 Origanum vulgare subsp. gracile

(K.Koch) Lestw.

Lamiaceae L 1,0-2,3 [17]

10 Pinus koraiensis Siebold & Zucc. Pinaceae L 11.52 [22]

11 Rosmarinus officinalis L. Lamiaceae L,

cnh

0,2-1,0 1,1-6,3 [10]

12 Rosmarinus tournefortii (Noe ex Jord.

& Fourr.) Jahand. & Maire

Lamiaceae PTMD 5,40 [27]

13 Salvia candelabrum Boiss. Lamiaceae L 0,25 4,4 [20]

14 Salvia fruticosa Mill. Lamiaceae L 0,46 1,00 [20]

15 Salvia officinalis subsp. lavandulifolia

(Vahl) Gams.

Lamiaceae L 0,89 2,2 [20]

11

TT

Tn khoa hc

H

Tn

Vit

Nam

B

phn

khai

thc

Hm

lng

TD

(%)

T l

Borneol

(%)

TL

TK

16 Salvia officinalis L. Lamiaceae Xn,

Cu tho

L 1,3-2,6 0,6-7 [10]

17 Salvia tomentosa Mill. Lamiaceae L 0,39 9,3 [20]

17 Satureja kitaibelii Wierzb. ex Heuff Lamiaceae PTMD 8,91 [32]

19 Salvia macrochlamys Boiss. & Kotschy Lamiaceae L 13 [33]

20 Thymus algeriensis Boiss. & Reut Lamiaceae PTMD 23,48 [16]

12

iu ch t tng hp ha hc

Borneol tng hp bng cch kh ha Camphor (Hnh 1.2) hoc Pinen

(t tinh du thng) cho hn hp ng phn Borneol vi thnh phn ch yu l

Isoborneol [4], [7].

Hnh 1.2: S tng hp Borneol t Camphor

1.2.3. Tc dng, cng dng

Trong y hc c truyn

Bng phin c dng trong ng y t lu i, c xp vo nhm

thuc Phng hng khai khiu.

- Tnh v: v cay, ng, tnh hi hn.

- Quy kinh: tm, t, ph.

- Cng nng ch tr:

Khai khiu tnh thn: dng i vi hu hng sng au, au rng, phi

hp vi phn phi, hong b, ng tm tho.

Tiu tn mng mng: dng khi mt au , mt c mng mng.

- Liu dng: 0,22-0,44g [9].

Ngoi ra, Bng phin cn dng bi ngoi cha mn nht l lot; pha

cn dng xoa bp cha: thp khp, chn thng t mu, st trng ngoi da

[1], [7].

13

Trong y hc hin i

- Tc dng lm tng tnh thm, ci thin sinh kh dng ca thuc dng

phi hp:

Borneol lm tng tnh thm qua gic mc ca thuc thn lipid:

Indomethacin v Dexamethasone l 1,67; 2,00 ln; thuc thn nc:

Ofloxacin, Ribavirin v Tobramycin l 2,15; 2,18 v 3,39 ln. Mt khc, cc

biu m gic mc khng b tn thng v suy yu trc tc ng tng cng

ca Borneol. Borneol l hot cht tim nng c s dng nhm tng s hp

thu ca thuc qua hng ro mu-mt [18].

Borneol lm ko di thi gian gy m ca 2,6-Diisopropylphenol khi s

dng kt hp do Borneol c ch s chuyn ha ca 2,6-Diisopropylphenol

qua gan bng con ng lin hp vi acid Glucuronic [14].

Borneol lm tng s hp thu ca Gastrodin qua ng tiu ha v thc

y phn b n no. So vi vic s dng Gastrodin mt mnh, vic dng kt

hp vi Borneol c th c hp thu nhanh qua ng tiu ha, thi gian t

nng ln nht ca Gastrodin trong huyt tng ngn hn l 5 15 pht so

vi bnh thng l 30 pht, sinh kh dng ca Gastrodigenin trong no tng

33,6%-108,8% [37].

- Tc dng chng vim, st khun

Tinh du i bi c tc dng c ch hot ng ca Bacillus cereus

nng c ch ti thiu (MIC) l 150g/ml, Staphylococus aureaus v

Candida albicans nng c ch ti thiu (MIC) l 120g/ml [35].

- Tc dng gim au, gy m:

Lin quan n tc dng trn cc th th GABA: receptors GABA(A)

kt hp (1) (2) (2L); p ng ca GABA t nht l tng ng vi cc

thuc gy m Etomidate v ln hn nhiu so vi Diazepam hoc 5-pregnan-

3-ol-20-one [29]. Mt khc, Borneol cn c tc dng c ch receptor

14

Nicotinic acetylcholine (nAChR), c ch nAChR qua ch vn 1,1-dimethy-4-

phenyl piperazinium iodide vi IC50= 56 9 M. Tc dng c ch nAChR

ca Borneol mnh hn Lidocain, v theo phng thc khng cnh tranh [28].

- Tc dng h huyt p:

Borneol c tc dng h ng k huyt p trn th, lm gim s peroxy

ha lipid, tng hot ng v nng cc enzyme chng oxy ha [23].

- Tc dng chng ng mu:

Nh c tnh ko di cc thng s ng mu nh thi gian Prothrombin

(PT) v thi gian Thrombin (TT) m khng tc ng ln hot ng chng kt

tp tiu cu, Borneol c tc dng chng ng mu; c s dng trong cng

thc phi hp nhm phng nga cc bnh v tim mch [36].

Hin nay, Borneol c dng h tr iu tr cc bnh v phi, dng

bi ti ch kt hp vi cc dng thuc khc nhm iu tr cc chng bnh:

sng hng, l ming, nhim trng tai, bnh vy nn, mn nht, bnh v mt

v thp khp. Dung dch Borneol dng tim, ung tr tim v st khun [4].

15

CHNG 2: I TNG V PHNG PHP

NGHIN CU

2.1. Nguyn vt liu, thit b

2.1.1. Nguyn liu nghin cu

Mu cy Blumea balsamifera (L.) DC. c thu hi ti Yn Ninh Ph

Lng Thi Nguyn. Cc mu cy c ngun gc khc nhau, c ly ging

t cc tnh khc nhau min Bc: H Ni, H Giang, in Bin, Thi

Nguyn. Bt u t thng 3 nm 2011, cc mu c trng ti mt a im

(x Yn Ninh huyn Ph Lng tnh Thi Nguyn) vi cng mt iu

kin chm sc. Khong cch gia cc mu l 2,5 m. Ti thi im bt u

nghin cu, cc mu cao khong 2,5 m. Thi im ly mu trong ngy: 12h-

13h.

C tng cng 8 mu c thu, x l kh, c gim nh tn khoa

hc v lu tr ti Phng tiu bn B mn Thc vt Trng i hc Dc

H Ni (HNIP) (Bng 2.1).

Bng 2.1. Danh sch mu i bi

TT S hiu a im ly ging

1 A1 Yn Ninh Ph Lng Thi Nguyn

2 A2 V Xuyn H Giang

3 A3 ng M Sn Ty H Ni

4 A4 Qun B - H Giang

5 A5 Thanh Lung in Bin in Bin

6 A6 Yn Bi Ba V H Ni

7 A7 Tng B V Xuyn H Giang

8 A8 Bc M H Giang

16

- Cc mu c m t c im hnh thi phn trn mt t bao gm:

C quan sinh dng v c quan sinh sn.

- Nghin cu hm lng borneol: l v cnh non cc mu cy i bi

c thu hi vo tun th 4 ca cc thng 9, 10, 11, 12 nm 2012 v cc

thng 1, 2, 3, 4 nm 2013. Nguyn liu c phi kh v bo qun trong ti

nylon.

2.1.2. Thit b v ha cht nghin cu

Thit b:

- Knh hin vi Leica, knh lp soi ni Leica, my nh Canon IXY 30S,

t sy Shellab, kim mi mc, chi lng, phin knh, lamen,...

- B nh lng tinh du nh hn nc ca B mn Thc vt i

hc Dc H Ni.

- B my phn tch GC-MS:

My Agilent Technologies 6890N, detector Agilent Technologies

5973i; ct HP-5MS (30m x 0,25mm x 0,25m).

Ha cht:

- Nc Javen, acid acetic, xanh methylen, son phn, nc ct lm

tiu bn,...

2.2. Ni dung nghin cu

2.2.1. Nghin cu c im thc vt

Nghin cu v c im hnh thi v v cu to gii phu ca 8 mu i

bi. nh gi s khc nhau v c im thc vt ca cc mu trong cng mt

iu kin sinh thi.

2.2.2. Nghin cu hm lng Borneol

Nghin cu s khc nhau v hm lng Borneol ca cc mu i bi

trong cng mt iu kin sinh thi v s thay i hm lng Mai hoa bng

phin trong l i bi theo thi gian.

17

- nh lng hm lng Borneol trong Mai hoa bng phin v trong

dc liu kh tuyt i ca 8 mu i bi thu hi vo thng 11 nm 2012.

- nh lng hm lng Mai hoa bng phin trong dc liu kh tuyt

i ca 8 mu i bi vo cc thng 9, 10, 11, 12 nm 2012 v cc thng 1, 2,

3, 4 nm 2013.

2.3. Phng php thc nghim

2.3.1. Nghin cu c im thc vt

- Nghin cu v c im hnh thi c quan st bng mt thng,

knh lp soi ni v m t theo phng php m t phn tch.

- Nghin cu v cu to gii phu: s dng phng php nhum kp,

quan st trn knh hin vi, m t theo phng php m t phn tch.

2.3.2. Nghin cu hm lng Borneol

nh lng Mai hoa bng phin trong i bi

Nguyn liu c ct nh, ct tinh du bng b nh lng tinh du nh

hn nc, theo phng php ct ko hi nc, cho n khi khng cn tinh

du trong dc liu, Mai hoa bng phin thu c di dng rn, em cn

xc dnh hm lng MHBP.

Song song tin hnh xc nh hm m dc liu theo phng php

nhit , s dng my o hm m nhit l 100C.

Hm lng Mai hoa bng phin trong dc liu kh tuyt i c xc

nh theo cng thc:

Trong : a: khi lng Mai hoa bng phin (g)

H: hm m dc liu (%)

m: khi lng nguyn liu (g)

18

X: hm lng Mai hoa bng phin (%)

Xc nh hm lng Borneol trong dc liu

Phn tch thnh phn tinh du bng phng php sc k kh khi ph

(GC-MS) ti Vin Php y Qun i. iu kin phn tch: Tc dng kh He

0,9 ml/pht, nhit bung bm mu 265C, nhit detector 280C, nhit

MS source 230C, nhit quard 150C. Chng trnh nhit ca ct l:

70C gi 2 pht, tng 30C/pht n 190C, tng tip 5C/pht n 270C,

gi 15 pht. Bm mu theo ch chia dng 10:1, th tch bm mu 1l.

Hm lng Borneol trong dc liu c tnh theo cng thc:

Y= X.A

Trong :

Y: Hm lng Borneol trong dc liu (%)

X: Hm lng MHBP trong dc liu (%)

A: T l Borneol trong MHBP (%)

19

CHNG 3: THC NGHIM, KT QU V BN LUN

3.1. Kt qu thc nghim

3.1.1. c im thc vt v hm lngBorneol ca cc mu i bi

c im hnh thi

Cc mu i bi thu c c c im hnh thi tng i ging nhau,

cc c im chung ca cc mu c m t nh sau:

Cy bi, cao 1-3m, mu xanh phn thn non, nu xanh phn thn gi.

Thn tit din gn trn, c kha dc, ng knh 5-10 mm. Ton cy c mi

thm v c lng t mn. Phn cnh dng ng pha ngn. L n, mc cch.

Phin l thun di, kch thc 8-27 x 2,0-7,6 cm, mt trn mu xanh m, t

lng hn mt di. Mt di l ph nhiu lng mt. Mp l x rng ca.

Gn l hnh lng chim ni r mt di, c 9-17 cp gn.Cung l hnh tr,

mu xanh, hi phng mt trn; mang 1-4 cp ph b kch thc nh dn v

pha y cung (Hnh 3.1). Cm hoa u thng hp thnh chm xim ngn

cnh hay nch l, kch thc rt thay i. u hnh trng khi l n, khi hoa n

u c hnh chung hi tht gia, kch thc khong 0,61,0 x 0,30,5 cm;

cung ngn 0,10,6 cm, c 1-3 vy ngn. Tng bao l bc 57 hng l bc

xp kt lp, hnh bu dc n di hp, vng trong di hn vng ngoi, kch

thc 15 x 0,20,5 mm, mu xanh, nh mu nu hoc nu tm, ra mng,

mt ngoi c nhiu lng. cm hoa hi li, ng knh khong 2 4 mm,

l t ong. u mang hoa hnh ng, c hai loi hoa: hoa ci rt nhiu xp 3-4

vng ngoi, hoa lng tnh 18-25 hoa trong (Hnh 3.2). Hoa ci mu vng

nht, nhng hoa ngoi cng thng c hnh tr cong; i bin i thnh tm

lng di mu trng; trng hoa mu vng nht, dnh thnh mt ng hp, di 4-

6mm, trn chia 2-3 thy tam gic nh, c lng tit tinh du thm; bu di 1

20

, mu trng trong m, hnh tr hp gc; vi nhy vng nht, dng si di

khong 57 mm, tn cng l 2 nhnh u nhy mu vng m hnh mng hp

di 12 mm. Hoa lng tnh ln hn hoa ci, mu vng nht; trng hoa hnh

ng di, loe dn, trn chia 5 thy hnh tam gic c mu vng m; nh 5, ch

nh ri, dng si, di khong 12 mm, mu vng nht, bao phn dnh nhau

thnh ng di khong 23 mm, bao ly vi nhy, mu vng m; vi nhy

dng si di khong 57 mm, u nhy 2, di khong 12 mm, mu vng

m, dng mng cn, thun hp. Qu b mu vng nu, hnh tr di khong

0,51mm, mt ngoi v c lng ngn mu trng v c 4-6 g dc, trn mang

tm lng mo di, mu trng ca i tn ti, gc c vng g ch gn vi

hoa; 1 ht (Hnh 3.3).

1: Cnh, 2: Chiu di l, 3: Chiu rng l

Hnh 3.1: c im hnh thi c quan sinh dng

21

1: Cm hoa; 2: L bc; 3: Hoa ci v hoa lng tnh; 4: cm hoa ct dc

Hnh 3.2: c im hnh thi c quan sinh sn (1)

22

1: u ng trng hoa lng tnh, 2: Ch nh v bao phn,

3: Bao phn ct dc, 4: Qu mang mo lng

Hnh3.3: c im hnh thi c quan sinh sn (2)

23

Hnh thi cc mu i bi c mt s c im khc nhau (Bng 3.1):

Bng 3.1: Cc c im hnh thi khc nhau gia cc mu

c im M t A1 A2 A3 A4 A5 A6 A7 A8

Mu sc thn gn im phn cnh (Hnh 3.4)

Xanh

x x x x x x x

Hi tm

x

Mt lng che ch (Hnh 3.6)

t x x x x

Nhiu x x x x

Hnh thi l qua cc giai

on (Hnh 3.5) Thay

i x x

Khng

i x x x x x x

Mu sc nh thy ca hoa lng tnh (Hnh 3.8)

Vng x x x x x x x

Hi tm

x

c im ngn l bc (Hnh 3.7)

Mu

nu

x x x x x

Mu

nu

tm

x x x

T l trung bnh chiu di l/ chiu rng l

5,4 3,7 6,2 4,4 6,7 3,6 3,6 5,0

24

1: Thn mu xanh, 2: Thn mu tm Hnh 3.5: S thay i hnh

Hnh 3.4: S khc nhau v mu sc thi l cc giai on

thn gn im phn cnh

1: Lng che ch t v ngn, 2: Lng che ch nhiu v di

Hnh 3.6: So snh mt lng che ch

1 2

1 2

25

Mu nu tm Mu nu

Hnh 3.7: c im ngn l bc

Mu tm hng Mu vng

Hnh 3.8: c im mu sc u trng hoa lng tnh

26

c im vi phu

c im vi phu ca cc mu khng c s khc bit, c m t nh sau:

- Vi phu thn: Hnh 3.9.

2 1 6 7 4

5 5

10

8 8 8

9 9

11 10

3 3

1: Biu b; 2: Lng che ch; 3: Lng tit a bo; 4: M dy; 5: T bo tit

tinh du; 6: M cng; 7: Libe; 8: Tng pht sinh libe - g; 9: G; 10: M mm

rut.

Hnh 3.9: c im vi phu thn

Mt ct ca thn c hnh gn trn, t ngoi vo trong bao gm:

(1) Lp biu b: l mt lp t bo nh xp ngoi cng, hnh ch nht

hoc hnh trn, khng u. Trn lp biu b c nhiu lng che ch a bo v

lng tit a bo. (2) Lng che ch a bo c cu to gm mt dy 3 5 t bo

di pha trn v nhiu t bo nh, ngn xp trn 1 3 hng gn gc. (3)

Lng tit a bo c chn di hoc ngn, u hnh trng thun cu to bi

nhiu hng t bo c lp cutin ph thnh m ch. (4) M dy l 4 8 lp t

bo trn hoc a gic gn trn, bt mu hng m, kch thc khng u

nhau, ln hn hoc bng t bo biu b. (5) T bo tit tinh du ri rc trong

27

m dy, m mm v, m mm rut. Cc b libe-g xp theo mt vng trn.

(6) M cng xp thnh tng cm khng u trn libe. (7) Libe nhiu t bo

hnh a gic, kch thc nh, bt mu hng. (8) Tng pht sinh libe-g gia

libe v g, gm nhiu lp t bo nh, c vch t bo mng. (9) G c mch

g hnh trn, m mm g gm nhiu t bo hnh a gic vch cellulose. (10)

M mm rut chim mt vng rng, gm nhiu t bo hnh trn hoc gn

trn, kch thc khng u.

- Vi phu gn l: Hnh 3.10.

6

6 5

5

2

4

7

1 3 8

1: Biu b; 2: Lng tit a bo; 3: Lng che ch; 4: M dy; 5: Libe; 6: G; 7:

T bo tit; 8: M mm

Hnh 3.10: c im vi phu gn l

Gn l mt trn li cao hi bng nh, mt di phnh trn tht hai

bn phin l, i khi c g li ph. (1) T bo biu b hnh bu dc hay ch

nht kch thc khng u, c rt nhiu lng che ch v lng tit a bo. (2)

Lng tit bo v (3) lng che ch c cu trc ging thn. (4) M dy: 5 7

28

lp t bo di biu b trn v 1-6 lp t bo trn biu b di, t bo kch

thc khng u. (5) Libe: t bo kch thc nh, hnh a gic, xp ln xn,

bt mu hng. (6) Mch g hnh trn, xp thnh 3-8 dy xen k vi 1-2 dy t

bo m mm g vch cellulose. (7) T bo tit ri rc trong m mm. (8) M

mm: gm nhiu lp t bo hnh trn, thnh mng.

- Vi phu phin l: Hnh 3.11.

1 1

3 3

5 5

6 2 4

1: Biu b trn; 2: Biu b di; 3 M du; 4: Lng che ch;

5: Lng tit; 6: T bo tit

Hnh 3.11: c im vi phu phin l

(1) Biu b trn t bo hnh bu dc, kch thc khng u. (2) Biu b

di t bo c kch thc nh hn biu b trn nhiu ln. C 2 lp biu b u

c nhiu lng che ch v lng tit a bo c cu to ging nh thn. (3) M

giu n su vo phn gn gia, gm 1 lp t bo. T bo m giu hnh ch

nht c chiu di gp 3 ln chiu rng.T bo tit nm ri rc trn phin l.

Hm lng Borneol

Cc mu Mai hoa bng phin ca 8 mu i bi thu hi vo thng 11

nm 2012 c phn tch cho thy: tt c cc mu u cha Borneol v

Camphor. Pic sc k ca cc mu MHBP c trnh by Ph lc 3.

29

T l Borneol trong cc mu Mai hoa bng phin thay i t 95,6% -

99,7% (Bng 3.2).

Bng 3.2: T l Borneol trong Mai hoa bng phin (thng 11)

Mu T l Borneol (%)

A1 99,5

A2 99,3

A3 95,6

A4 99,2

A5 98,3

A6 98,3

A7 99,7

A8 99,5

Hm lng Borneol trong dc liu kh tuyt i ca cc mu thay i

t 0,63%-0,84% (Bng 3.3).

Bng 3.3: Hm lng Borneol trong cc mu (thng 11)

Mu Hm lng Borneol (%)

A1 0,84

A2 0,67

A3 0,73

A4 0,64

A5 0,73

A6 0,68

A7 0,68

A8 0,63

30

Hm lng Borneol trong dc liu kh tuyt i ca cc mu c s

chnh lch khng nhiu (Hnh 3.12).

Hnh 3.12: Biu hm lng Borneol trong dc liu

kh tuyt i ca cc mu (thng 11)

3.1.2. S thay i hm lng Mai hoa bng phin theo thi gian

Kt qu nh lng hm lng Mai hoa bng phin trong dc liu kh

tuyt i ca cc mu i bi vo cc thng 9, 10, 11, 12 nm 2012 v cc

thng 1, 2, 3, 4 nm 2013 nh sau (Bng 3.4):

,000%

,100%

,200%

,300%

,400%

,500%

,600%

,700%

,800%

,900%

A1 A2 A3 A4 A5 A6 A7 A8

%Borneol

%Borneol

Mu

31

Bng 3.4: Hm lng Mai hoa bng phin trong cc mu nghin cu

Mu

Nm 2012 Nm 2013

Thng

9 (%)

Thng

10 (%)

Thng

11 (%)

Thng

12 (%)

Thng

1 (%)

Thng

2 (%)

Thng

3 (%)

Thng

4 (%)

A1 1,54 1,51 0,84 0,78 0,52 0,44 0,42 0,61

A2 1,40 1,41 0,67 0,39 0,29 0,26 0,23 0,36

A3 1,37 1,36 0,76 0,74 0,26 0,24 0,23 0,33

A4 1,31 0,72 0,65 0,39 0,31 0,25 0,22 0,39

A5 1,15 1,02 0,74 0,45 0,29 0,28 0,23 0,29

A6 1,12 1,00 0,69 0,68 0,32 0,25 0,24 0,34

A7 1,08 0,94 0,68 0,38 0,28 0,24 0,22 0,38

A8 1,00 0,89 0,63 0,49 0,30 0,28 0,26 0,35

Hm lng Mai hoa bng phin trong dc liu kh tuyt i ca cc

mu i bi nm trong khong 0,22% - 1,54%. Cc mu i bi c hm lng

MHBP cao nht vo thng 9/2012, gim dn v thp nht vo thng 3/2013 l

thi im cy ra hoa, sau li tng ln vo thng 4/2013 l thi im cy

u qu (Hnh 3.13).

32

Hnh 3.13: Biu s thay i hm lng Mai hoa bng phin

8 mu i bi theo thi gian

3.2. Bn lun

Theo mt nghin cu v tnh a dng sinh hc cy i bi min Bc

Vit Nam, cc cy thuc loi Blumea balsamifera (L.) DC. kh a dng. Vi

cng mt thi im thu hi, cc mu i bi thu hi cc a im khc nhau

c s khc bit ln v hm lng tinh du (0,32%-2,19%) v hm lng

Borneol trong tinh du (3,34%-82,23%) [10]. c nh cc bin v iu

kin kh hu, th nhng v chm sc, cc mu i bi c ly ging t cc

tnh khc nhau: H Giang, H Ni, in Bin, Thi Nguyn; trng ti mt a

im (Yn Ninh Ph Lng Thi Nguyn).

,000%

,200%

,400%

,600%

,800%

1,000%

1,200%

1,400%

1,600%

1,800%

T9 T10 T11 T12 T1 T2 T3 T4

A1

A2

A3

A4

A5

A6

A7

A8

%Borneol

Thi gian

33

Kt qu nghin cu v hnh thi cho thy cc mu i bi c s khc

nhau v mu sc thn, mu sc nh thy hoa lng tnh, c im ngn l

bc, mt lng che ch. Kt qu nh lng hm lng v thnh phn Mai

hoa bng phin ca cc mu i bi ti cng mt thi im thu hi (thng 11

nm 2012) khng c s khc bit r rt: Hm lng Mai hoa bng phin t

0,63%-0,84%; hm lng Borneol trong MHBP t 95,6%-99,7%; hm lng

Borneol trong dc liu kh tuyt i ca cc mu t 0,63%-0,84%. Nh vy,

sau khi c nh cc bin thuc iu kin sinh thi th s sai khc v hm

lng MHBP v Borneol gia cc mu gim i ng k, cc mu c xu

hng ging nhau v hm lng v thnh phn MHBP. Kt qu ny bc u

ch ra rng iu kin sinh thi c nh hng nhiu n hm lng v thnh

phn tinh du i bi. Tuy nhin, ti thi im hm lng MHBP ln nht

(thng 9), hm lng MHBP trong cc mu i bi c s khc bit r rt hn

(1,00%-1,54%). iu ny cho thy yu t ngun gen c nh hng n hm

lng MHBP ca cc mu i bi.

Kt qu nh lng hm lng Mai hoa bng phin cho thy hm lng

MHBP ca cc mu i bi cao nht vo thng 9 nm 2012 (1,00% - 1,54%),

gim dn v thp nht vo thng 3 nm 2013 (0,22 0,42%), sau li tng

ln vo thng 4 nm 2013 (0,29%-0,61%). Thi im hm lng MHBP thp

nht l ma xun, lc cy ra hoa v thi im hm lng MHBP cao nht l

ma thu, iu ny ph hp vi kinh nghim thu hi i bi ca nhn dn ta v

mt nghin cu trc ti Vit Nam [8]. Tuy nhin, cn phi tip tc nh

lng hm lng Mai hoa bng phin vo cc thng 5, 6, 7, 8 ca nm 2013

v thnh phn MHBP ca tt c cc thng trong nm tm ra thi im thu

hi i bi ti u nht.

Kt qu phn tch thnh phn tinh du cho thy hm lng Borneol

trong Mai hoa bng phin ca cc mu t 95,6% - 99,7% l rt cao, trong khi

34

hm lng ca Borneol trong tinh du ca mt s cy c s dng ct

trong cng nghip nh Dipterocarpus turbinatus C. F. Gaertn.

(Dipterocarpaceae) 30%-65% [40], Amomum villosum var. xanthioides (Wall.

ex Baker) T.L.Wu & S.J.Chen (Zingiberaceae) 19% [8], Micromeria cristata

(Hampe) Griseb.subsp. phrygia P. H. Davis (Lamiaceae) 27% - 39% [40].

Chng t y l ngun nguyn liu ph hp dng khai thc Borneol thin

nhin. Hm lng Borneol trong Mai hoa bng phin ca tt c cc mu

nghin cu ln hn nhiu so vi hm lng Borneol trong tinh du ca cc

mu i bi trong cc nghin cu trc ti Vit Nam (3,34% - 82,23%)

[14], [10], [7]. Do cn tip tc nghin cu cc c im sinh thi c lin

quan n hm lng Borneol to c vng trng nguyn liu khai thc

Borneol t cy i bi.

KT LUN V KIN NGH

Kt lun:

Nghin cu m t chi tit c c im hnh thi, vi phu ca 8 mu

i bi c ngun gc khc nhau t H Ni, H Giang, Thi Nguyn, in Bin

c trng ti cng mt a im (Yn Ninh - Ph Lng - Thi Nguyn) c

s khc nhau v hnh thi: mu sc thn, mu sc nh trng hoa lng tnh,

mu sc nh l bc, mt lng che ch, Vi cng mt thi im thu hi

l thng 11/2012, 8 mu i bi khng c s khc bit r rt v hm lng

Borneol trong dc liu kh tuyt i (0,63%-0,84%). Hm lng Borneol

trong MHBP ca cc mu xp x bng nhau v rt cao (95,6%-99,7%).

Nghin cu s thay i hm lng Mai hoa bng phin trong cc mu

i bi theo thi gian t thng 9/2012 n thng 4/2013: hm lng MHBP

trong dc liu kh tuyt i cao nht vo thng 9/2012 (1,00% - 1,54%),

gim dn v thp nht vo thng 3/2013 (0,22% 0,42%) l thi im cy ra

35

hoa, sau li tng ln vo thng 4/2013 (0,29%-0,61%) l thi im cy u

qu.

Kin ngh:

Trn y l cc kt qu bc u m chng ti t c trong iu kin

thi gian v kh nng cho php. tip tc thc hin ti, chng ti kin

ngh:

- Nghin cu s thay i hm lng Borneol trong MHBP theo thi

gian v nh lng hm lng MHBP trong cc mu vo cc thng 5, 6, 7, 8

ca nm 2013.

- Theo di , kim tra s thay i v hnh thi, hm lng Borneol ca

cc mu i bi c cng ngun gc trong cc iu kin sinh thi khc nhau.

- Nghin cu s thay i hm lng Borneol trong l i bi theo s

pht trin ca l: L non, l bnh t v l gi.

TI LIU THAM KHO

Ti liu ting Vit

1. Huy Bch, ng Quang Trung v cng s (2004), Nhng cy thuc v

ng vt lm thuc Vit Nam, NXB Khoa hc v K thut, trang 722-724.

2. L Kim Bin (2007), Thc vt ch Vit Nam, NXB Khoa hc v K thut,

tp 7, tr. 218-219.

3. V Vn Chi (1997), T in cy thuc Vit Nam, NXB Y hc, trang 1461.

4. Nguyn Duy Cng, Nguyn Hu Qunh v cng s (1999), T in bch

khoa dc hc, NXB T in bch khoa H Ni, tr. 80.

5. Phm Thanh K (2007), Dc liu hc tp 2, NXB Y hc, tr. 174-187, tr.

240-241.

6. Tt Li (1999), Nhng cy thuc v v thuc Vit Nam, NXB Y hc, tr.

605-607, tr. 402-403.

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Ti liu trn Internet

http://www.efloras.org/florataxon.aspx?flora_id=3&taxon_id=200023545

PH LC 1

TRNG I HC DC H NI

B MN THC VT

*****

PHIU GIM NH TN KHOA HC

S: 02/2013

Ngi thu mu: Phan Th An

Ngi gi mu: Phan Th An A3K63 H Dc H Ni

Ngy thu mu: (Bng km theo)

Ni thu mu: (Bng km theo)

Tn a phng:

Yu cu: Gim nh tn khoa hc

M t mu: 8 mu, gm thn mang l, hoa

Kt qu gim nh: Cn c vo cc ti liu thc vt hin c ti Trng H Dc

H Ni (bao gm: Thc vt ch Vit Nam H Asteraceae; Cy c Vit Nam Tp

3; T in Thc vt thng dng Tp 1; Flora of China, Vol. 20-21;Flora of

Taiwan Compositae;Flora Reipublicae Popularis Sinicae Tomus 75; v cc

mu lu ti phng tiu bn National Taiwan University TAI; Herbier Musum

Paris Musum National dHistoire Naturelle vi cc c im ca cc b phn

mu cy, xc nh mu trn c:

- Tn khoa hc: Blumea balsamifera (L.) DC.

- H: Asteraceae

- Tn thng gi: i Bi

H Ni, ngy 16 thng 05 nm 2013

B MN THC VT

Ngi gim nh

DS. Nghim c Trng

Bng danh sch cc cy gi gim nh

TT Ni thu mu Ngy thu mu K hiu

mu Ngi thu mu

1 Yn Ninh Ph Lng Thi Nguyn

30/03/2013 A1

Phan Th An


30/03/2013 A2

Phan Th An


30/03/2013 A3

Phan Th An


30/03/2013 A4

Phan Th An


30/03/2013 A5

Phan Th An


30/03/2013 A6

Phan Th An


30/03/2013 A7

Phan Th An


30/03/2013 A8

Phan Th An

TRNG I HC DC H NI

B MN THC VT

PHNG TIU BN CY THUC (HNIP)

***

GIY CHNG NHN M S TIU BN

1. Tn mu cy

- Tn khoa hc: (bng km theo)

- Tn thng dng: (bng km theo)

- Tn a phng: (bng km theo)

2. Ngun gc: Yn Ninh Ph Lng Thi Nguyn

3. Ngy thu mu: (bng km theo)

4. Ngy np mu: 16/05/2013

5. Ngi thu mu: Phan Th An C quan: A3K63, H Dc HN

6. Ngi np mu: Phan Th An C quan: A3K63, H Dc HN

7. S hiu phng tiu bn: (bng km theo)

8. Ngi gim nh tn khoa hc: DS. Nghim c Trng

9. S lng mu np: 23

Ngi np mu

Phan Th An

Ngi nhn mu

TS. Nguyn Quc Huy

T

T MS Tn khoa hc H

Tn

thng gi

Tn a phng

a im ly ging MSTB

S TB

1 A1

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi Ph Lng-Thi Nguyn

HNIP/18041

/13 3

2 A2

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi V Xuyn-H Giang

HNIP/18042

/13 3

3 A3

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi Sn Ty-H Ni

HNIP/18043

/13 3

4 A4

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi Qun B-H Giang

HNIP/18044

/13 3

5 A5

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi

Thanh

Lung-in Bin

HNIP/18045

/13 2

6 A6

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi Ba V-H

Ni

HNIP/18046

/13 3

7 A7

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi

Tng B-V Xuyn-H

Giang

HNIP/18047

/13 3

8 A8

Blumea

balsamifera (L.)

DC.

Asteraceae i bi i ngi Bc M-H Giang

HNIP/18048

/13 3

PH LC 3

PIC SC K GC-MS CC MU MAI HOA BNG PHIN

Pic sc k GC-MS Mai hoa bng phin mu A1

1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

1 0 0 0 0 0 0

2 0 0 0 0 0 0

3 0 0 0 0 0 0

4 0 0 0 0 0 0

5 0 0 0 0 0 0

6 0 0 0 0 0 0

7 0 0 0 0 0 0

8 0 0 0 0 0 0

9 0 0 0 0 0 0

1 e + 0 7

1 . 1 e + 0 7

1 . 2 e + 0 7

T i m e - - >

A b u n d a n c e

T I C : 9 5 B O R N E O L A 1 L A N 2 . D \ d a t a . m s

1 4 . 4 3 7

1 5 . 0 5 7



1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

1 0 0 0 0 0 0

2 0 0 0 0 0 0

3 0 0 0 0 0 0

4 0 0 0 0 0 0

5 0 0 0 0 0 0

6 0 0 0 0 0 0

7 0 0 0 0 0 0

8 0 0 0 0 0 0

9 0 0 0 0 0 0

1 e + 0 7

1 . 1 e + 0 7

T i m e - - >

A b u n d a n c e

T I C : 9 5 B O R N E O L A 2 . D \ d a t a . m s

1 4 . 4 3 0

1 5 . 0 4 6

1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

5 0 0 0 0 0

1 0 0 0 0 0 0

1 5 0 0 0 0 0

2 0 0 0 0 0 0

2 5 0 0 0 0 0

3 0 0 0 0 0 0

3 5 0 0 0 0 0

4 0 0 0 0 0 0

4 5 0 0 0 0 0

5 0 0 0 0 0 0

5 5 0 0 0 0 0

6 0 0 0 0 0 0

6 5 0 0 0 0 0

7 0 0 0 0 0 0

T im e - - >

A b u n d a n c e


1 4 . 4 1 6

1 5 . 0 2 1



8 . 0 0 1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

1 0 0 0 0 0 0

2 0 0 0 0 0 0

3 0 0 0 0 0 0

4 0 0 0 0 0 0

5 0 0 0 0 0 0

6 0 0 0 0 0 0

7 0 0 0 0 0 0

8 0 0 0 0 0 0

9 0 0 0 0 0 0

1 e + 0 7

1 . 1 e + 0 7

1 . 2 e + 0 7

T i m e - - >

A b u n d a n c e


1 4 . 4 3 0

1 5 . 0 6 5

1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

1 0 0 0 0 0 0

2 0 0 0 0 0 0

3 0 0 0 0 0 0

4 0 0 0 0 0 0

5 0 0 0 0 0 0

6 0 0 0 0 0 0

7 0 0 0 0 0 0

8 0 0 0 0 0 0

9 0 0 0 0 0 0

T i m e - - >

A b u n d a n c e


1 4 . 4 2 5

1 5 . 0 3 3



8 . 0 0 1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

1 0 0 0 0 0 0

2 0 0 0 0 0 0

3 0 0 0 0 0 0

4 0 0 0 0 0 0

5 0 0 0 0 0 0

6 0 0 0 0 0 0

7 0 0 0 0 0 0

8 0 0 0 0 0 0

9 0 0 0 0 0 0

T i m e - - >

A b u n d a n c e


1 4 . 4 2 7

1 5 . 0 4 0

8 . 0 0 1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

1 0 0 0 0 0 0

2 0 0 0 0 0 0

3 0 0 0 0 0 0

4 0 0 0 0 0 0

5 0 0 0 0 0 0

6 0 0 0 0 0 0

7 0 0 0 0 0 0

8 0 0 0 0 0 0

9 0 0 0 0 0 0

1 e + 0 7

1 . 1 e + 0 7

T i m e - - >

A b u n d a n c e


1 4 . 4 4 3

1 5 . 0 5 4


1 0 . 0 0 1 2 . 0 0 1 4 . 0 0 1 6 . 0 0 1 8 . 0 0 2 0 . 0 0 2 2 . 0 0 2 4 . 0 0 2 6 . 0 0 2 8 . 0 0

1 0 0 0 0 0 0

2 0 0 0 0 0 0

3 0 0 0 0 0 0

4 0 0 0 0 0 0

5 0 0 0 0 0 0

6 0 0 0 0 0 0

7 0 0 0 0 0 0

8 0 0 0 0 0 0

9 0 0 0 0 0 0

1 e + 0 7

1 . 1 e + 0 7

T i m e - - >

A b u n d a n c e


1 4 . 4 3 4

1 5 . 0 5 2

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