Enzyme Amylase Phương Pháp Sản Xuất Và Ứng Dụng

8/8/2019 Enzyme Amylase Phng Php Sn Xut V ng Dng

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Revie w Article: Enzyme Amylase: Phng Php Sn Xut V ng DngSVTH: L PHNG V

TM TTTinh bt l sn phm t nhin quan trng nht c nhiu ng dng trong k thut v

trong i sng con ngi. Nhiu nc trn th gii s dng ngun tinh bt t khoaity, la m, ng (sn), cn ring nc ta th s dng go v khoai m l ngun tinhbt ch yu. thc hin qu trnh thu phn tinh bt trong thc t sn xut ngita thng s dng enzyme amylase. Enzym amylase c th tm thy nhiu ngunkhc nhau nh amylase t thc vt, ng vt v VSV. Hin nay, cc nh sn xut cth s dng amylase c kh nng chu nhit cao m khng b mt hot tnh, chnghn amylase c tch chit t VSV. Ngoi ra, s dng amylase thy phn tinh

bt cn nng lng xc tc thp, khng yu cu cao v thit b s dng, gim chiph cho qu trnh tinh sch dch ng. Ngun amylase c th ly t mm thc,

mm i mch ( malt), ht bp ny mm, hay t nm mc, Nguyn liu cho snxut l go, bp, khoai m, y l nhng ngun nguyn liu d tm, r tin c thtm thy d dng nc ta. Nh nhng u im trn m enzyme amylase c ngdng rng ri trong nhiu ngnh cng nghip: ch bin thc phm, dt, y dc Do , vic nm r phng php sn xut enzyme amylase ng dng vo thc tsn xut l nhu cu cn thit.

GVHD : TS. TRNG TRNG NGN 1


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I. GII THIU:1. Enzyme Amylase.

Amylase l mt h enzyme rt ph bin trong th gii sinh vt . Cc enzyme nythuc nhm enzyme thy phn, xc tc phn gii lin kt ni phn t trong nhmpolysaccharide vi s tham gia ca nc:

RR + H-OH RH + ROHC 6 loi enzyme c xp vo 2 nhm: endoamylase (enzyme ni bo) vexoamylase (enzyme ngoi bo). (1)

Endoamylase gm c -amylase v nhm enzyme kh nhnh. Nhm enzymekh nhnh ny c chia thnh 2 loi: Kh trc tip l Pullulanase, kh gintip l Transglucosylase v maylo-1,6-glucosidase. Cc enzyme ny thy

phn cc lin kt bn trong ca chui polysaccharide. Exoamylase gm c -amylase v -amylase. y l nhng enzyme thy

phn tinh bt t u khng kh ca chui polysaccharide.2. Mi Trng Sn Xut Enzyme Amylase .2.1 Mi trng lng . mi trng lng, VSV s pht trin trn b mt mi trng, to thnh khun lcngn cch pha lng (mi trng) v pha kh (khng kh). y, VSV s s dngcht dinh dng t dung dch mi trng, O2 t khng kh, tin hnh qu trnh tnghp enzyme. Enzyme ngoi bo s c tch ra t sinh khi v ha tan vo dung

dch mi trng. Enzyme ni bo s nm trong sinh khi VSV.

Hnh 1: Ln men trong mi trng lng quy m phng th nghim (5 L)

(www.khoahoc.com.vn)



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2.2 Mi trng bn rn .

Phn ln cc nh my sn xut enzyme, khi nui cy VSV thu nhn enzyme, ngita thng s dng mi trng c . tng kh nng xm nhp ca khng kh votrong lng mi trng, ngi ta thng s dng cm, tru, ht ng cc lm mitrng. Vi sinh vt pht trin trn b mt mi trng, nhn cht dinh dng t htmi trng v sinh tng hp ra enzyme ni bo v ngoi bo. Cc enzyme ngoi

bo s thm thu vo trong cc ht mi trng, cn cc enzyme ni bo nm trongsinh khi vi sinh vt. Vi sinh vt khng ch pht trin trn b mt mi trng, ningn cch pha rn ( mi trng ) v pha kh ( khng kh ) m cn pht trin trn bmt ca cc ht mi trng nm hn trong lng mi trng. Mi trng nui cy

va c xp cao v va phi c m thch hp. Nu m qu cao s lm btmi trng li, khng kh khng th xm nhp vo trong lng mi trng, nu c m thp qu s khng thun li cho VSV pht trin. Thng thng ngi tathng to m khong 55-65% W l hp l. Nu s dng cm lm nguyn liuchnh nui cu VSV thu nhn enzyme, ngi ta phi cho thm 20-25% tru lm xp mi trng, to iu kin thun li khng kh d xm nhp vo lng mitrng. Phng php nui cy b mt bn rn (mi trng c) ny rt thch hpcho len men nm mc Asp.oryzae .(2)

A. B.

3. Nguyn Liu Sn Xut Enzyme Amylase .3.1 Nguyn liu to mi trng nui cy.

Nguyn liu s dng trong nui cy b mt thng l nhng nguyn liu c ngungc t nhin nh cm m, cm go, go, ng mnh, u nnh v cc loi ht ng cckhc.Trong cc loi nguyn liu trn, cm go, cm m c s dng nhiu hn c.Hai loi ny c y cc cht dinh dng cn thit cho VSV pht trin. Mt khckhi to mi trng, chng thng c tnh cht vt l rt thch hp va m bokhi kt dnh cn thit, va m bo lng khng kh lu chuyn trong khi nguyn

liu.


Hnh 2: Ln men trn mitrng rn.A: ln men k khtrong ni bng t nung, B:ln men hiu kh.


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Nguyn liu s dng trong nui cy b su (S dng mi trng hon ton lng):Nguyn liu nui cy ph bin l dch ng nh glucose, fructose, sacarose,pepton, nng thch hp khong 10 - 15%.(3)3.2 Chng vi sinh vt dng trong sn xut amylase.

Chng nm mcAsp. Oryzae, Asp.nigero Enzyme chit xut t nm mc: d dng loi b cc khun ty ca nm

trong mi trng sn xut enzyme.o Enzyme amylase th nm trong dung dch lc.

Cc chng vi khun l Bacillus subtilis, Bacillus licheniformis, Bacillusstearothermophilus o Enzyme chit xut t vi khun: cn tc cao, lm lnh bng my li tm

cho vic ny.o mi trng nui cy vi khun vo ng my li tm, v quay trong 20

pht 5.000 vng/pht. Phn gn ni trn mt, l chit xut enzyme th.

Bng 1: Tnh cht ca amylase thu nhn t cc ngun khc nhau.(Nguyn Tin Thng, Gao trnh cng ngh enzyme 2008)

Ngun thu nhn

Gii hn pH

(pH ti u)

Khi lung phn

t (Dalton)

Nhit ti

u (oC)

B. subtilis 4,5 6,5 48.000 60

B. licheniformis 5,0 9,0 22.500 90

B.stearothermophilus 4,0 5,2(3,0) 96.000 80

B. cereus 6,0 7,0 90.000 50 55

Pseudomonas 6,7 7,0 62.000 45

Ty 6,0 7,0 45.000

Malt 4,5 9,5 (5,5) 52.000 70

Aspergilus oryzae 5,0 51.000 50 60

II. PHNG PHP SN XUT ENZYME AMYLASE1. Theo Phng Php Nui Cy B Mt :

1.1. Qui trnh cng ngh :



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Vi sinh vt

Etanol

Hp thanh trng: di p sut hi 1-1,5 atm trong thi gian 45-60 pht.

Trn ging vi sinh vt: Sau khi lm ngui, tin hnh cy ging hoc rc bo t vomi trng thanh trng, thnh ng vi gi. Khi cy vo mi trng dinhdng, bo t s pht trin thnh t bo nm mc v to ra cc loi enzyme m tamong mun.K thut nui cy: Sau khi trn ging, mi trng c tri u ra cc khay vichiu di 2-3cm, ri c a vo phng nui cy 28-32oC, khng cn iu chnh

pH. Thi gian nui nm si thu nhn enzyme vo khong 36-60 gi. Qu trnh phttrin ca nm mc trong mi trng bn rn khi nui bng phng php b mt nytri qua cc giai on sau:

Giai on 1: giai on ny ko di 10-14 gi k t thi gian bt u nui

cy.o Nhit tng rt chm.o Si nm bt u hnh thnh v c mu trng hoc mu sa.o Thnh phn dinh dng bt u c s thay i.o Khi mi trng cn ri rc.o Enzyme mi bt u c hnh thnh.

Giai on 2: giai on ny ko di 14-18 gi.o Ton b bo t pht trin thnh si nm c mu trng xmo Mi trng c kt li kh cht.o

m mi trng gim dn.


Mi trng cm m

Trch ly bng nc

Thm tch d ch trch

Kt ta Amylase

Li tm

S kt ta

Ch phm Amylase

Nghin, bo qun


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o Nhit mi trng s tng nhanh c th ln ti 40-45oC.o Cc cht dinh dng bt u gim nhanh do s ng ho mnh ca nm

si.o Enzyme amylase c tng hp mnh.o Lng O2 trong khng kh gim v CO2 s tng dn

Giai on 3: giai on ny ko di 10-20 gi.o Qu trnh trao i cht yu dn, do mc gim cht dinh dng s

chm li.o Nhit ca khi mi trng gim, do lm gim lng khng kh mi

trng xung 20-25 th tch khng kh /th tch phng nui cy/ 1gi.1.2. Thu nhn sn phm sn xut enzym tinh khit ngi ta ph tin hnh nh sau:

Ton b khi lng enzym th amylase c em i nghin nh ph vthnh t bo v lm nh cc thnh phn ca ch phm th.

S dng nhng cht tr nghin (ct thch anh v bt thy tinh) khi nghin.Trc khi s dng ct thch anh v bt thy tinh phi c ra sch, sy kh nhit ln hn 1000C loi b nc v tiu dit VSV.Sau khi nghin mn, ngi ta cho nc vo trch ly enzym-amylase. C mt phn ch phm enzym th, ngi ta cho 4-5 phn nc,khuy nh v sau lc ly dch, phn b thu ring dng lm thc phm giasc (ch cn loi b ct thch anh v bt thy tinh ra khi hn hp b rimi cho gia sc n).Dch thu nhn c vn dng ch phm enzym th vtrong c cha nc, cc cht ha tan khc t khi mi trng nui cy.Dng cn v sunfat amon kt ta enzyme.Trong khi tin hnh kt ta,ngi ta phi lm lnh c dung dch enzym th v c nhng tc nhn kt ta trnh lm mt hot tnh enzyme.

Khi cho cht kt ta vo dung dich enzyme th, ngi ta tin hnh khuynh, sau yn trong iu kin nhit 4-70C. Theo thi gian, ccenzyme s c to kt ta v lng xung y, ngi ta tin hnh gn v lc

thu nhn kt ta dng paste ( m > 70%W). (4)

1.3 u v nhc imu im :

Nui cy b mt rt d thc hin. Quy trnh cng ngh thng khng phctp.

Lng enzyme c to thnh t nui cy b mt thng cao hn rt nhiuso vi nui cy chm.

Ch phm enzyme th (bao gm thnh phn mi trng sinh khi vi sinh

vt, enzyme v nc). Sau khi thu nhn rt d sy kh v d bo qun.



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Nui cy b mt khng cn s dng nhiu thit b phc tp. Trong trng hp b nhim cc vi sinh vt l, ta rt d dng x l. Mi

trng c l mi trng tnh, khng c s xo trn nn khu vc no b

nhim ta ch cn loi b khu vc khi ton b khi nui cy. Nhng khuvc khc s hon ton c an ton.

Nhc im : Phng php ny tn kh ln din tch cho nui cy. Trong phngphp ny vi sinh vt pht trin trn b mt mi trng (mi trng lng hoc mitrng bn rn) nn rt cn nhiu din tch. (5)

2. Theo Phng Php Nui Cy B Su:2.1 Qui trnh cng ngh :

Hp kh trng nhit 118 125 0C, thi gian 40 60 pht, ngui dn nhit bnh thng v tip ging vi sinh vt vo mi trng, t l ging a vo l 2 2,5 %. Sau qu trnh nui cy c thc hin theo 2 phng php: nui cy theochu k hay nui cy lin tc.

Nui cy theo chu k l phng php nui cy trong 1 thit b ln men. Sau1 chu k nui t 2 4 ngy 28 32 0C ngi ta thu nhn ton b dch nuicy nh l 1 loi ch phm enzyme th. Phng php ny khng i hi kthut cao nhng nng sut thp.

Nui cy lin tc l khc phc tnh trng trn. Qu trnh nui cy lin tc

c th nui cy trong 1 thit b, cng c th thc hin trong nhiu thit b.


Vi Sinh Vt


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Phng php ny c li l nu cht lng sn phm ra cui cng ra ta thunhn uc cha t yu cu t ra ta c th khc phc bng hai cch.

o

Cch th nht: lm cho thi gian lu ca dung dch v t bo vi sinhvt trong thit b lu hn.o Cch th hai l: ta tin hnh hon lu dch ln men ha chung vi

dng mi trng ti ln men.2.2 Thu nhn sn phmDung dch sau khi nui cy theo phng php b su c tch khi sinh khi vcc thnh phn khng ha tan bng phng php ly tm. Dch thu thng cha 2 3% cht kh ha tan. Hm lng cht ny rt nh, do ta cn phi c c chngcho n khi khi lng dch gim i 5 10 ln iu kin chn khng.

2.3 u v nhc imu im:

Phng php nui cy hin i d c kh ho, t ng ho, nng sut cao

C th nui cy d dng cc chng vi sinh vt t bin c kh nng sinh tnghp enzyme cao v la chn ti u thnh phn mi trng, cc iu kinnui cy, enzyme thu c tinh khit hn, m bo v trng.

Nhc im:

i hi phi c v trng tuyt i cc khu v sinh tng hp, thanh

trng mi trng dinh dng, thao tc nui cy, khng kh cung cp cho qutrnh nui cy.

Tn in nng cho khuy trn, nu khng bo m v trng s b nhim hng lot,

ton b gy tn thng ln v thu hi enzyme s c gi thnh cao. (5)

3. Cc Yu T nh Hng n Cht Lng Enzyme3.1 nh hng cc ngun Nit dinh dng

Bng 2: nh hng ca ngun Nit ti sinh tng hp cc enzyme amylase.( Sch Cng ngh enzyme ca Nguyn c Lng ch bin, 2004)

Ngun Nit Liu lng mui,% theo N

Hot ng enzyme sau 6 ngy nui ivi 100ml

-amylase GlucomylaseNaNO3 0,30 145 8000NaNO3 0,15 26 3300(NH4)2SO2 0,15 - -

NH4NO3 0,15 98 4100NH4NO3 0,30 45 4900NH4H2PO4 0,30 5,5 8000



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3.2 nh Hng Ca Ngun Khong Dinh Dng

- Mg

2+

c nh hng ti bn nhit ca enzym.- Phospho nh hng trc tip ti sinh sn ca nm si, do vy m tng cng tnghp enzyme.- Canxi(Ca) cn cho tng hp v n nh -amylase hot ng v n l cu t khngth thiu c ca enzym ny. Canxi cn c tc dng bo v amylase khi tc ngca protease ( Hsiu et amiloza, 1964).- Lu hunh(S) kch thch s to amylase.

Ngoi nhng nguyn t a lng ny cn ch thm vi nhng nguyn t vi lng.Chng hn nh: Co, km,v cc nguyn t gy c ch s tng hp enzym

amylase nh: ng, thy ngn, 4. Cc Yu T nh Hng n Qu Trnh Ln Men- m mi trng:Bng 3: nh hng ca gi m trong qu trnh sinh trng ti s to -amylase

ca Asp.oryzae nui bng phng php nui cy b mt(http://gbb.eldoc.ub.rug.nl/root/2002/JBiotechnolvdMaarel/)

Phng nth nghim

20 gi 34 gi 42 gi m

%

Hot

amylase ivi canh Itrng kh

m

%

Hot

amylase/v canhtrng

kh

m%

Hot

amylase ivi canh Itrng kh

Khay h 27,8 15,0 23,8 18,0 22,0 20,5Khay y np 46,4 20,4 42,4 32,9 42,4 36,7

- m tng i ca khng kh: m cho php t 80% tr ln n mc bo hotc l 100% W u thch hp cho VSV pht trin.- nh hng ca nhit : Nhit thch hp cho s pht trin v hnh thnhenzyme l 28-320C.- nh hng ca pH: pH thch hp cho VSVpht trin l mi trng acid yukhong 5,5-6.5. (6)

III. NG DNG CA ENZYME AMYLASE:1. ng Dng Ca Enzyme Amylase Trong Ch Bin Thc Phm Gia Sc:Trong ch bin thc n gia sc, thnh phn ng cc chim mt khi lng rt ln.

Trong khi lng ny, thnh phn tinh bt rt cao. tng hiu sut s dng nng

http://gbb.eldoc.ub.rug.nl/root/2002/JBiotechnolvdMaarel/http://gbb.eldoc.ub.rug.nl/root/2002/JBiotechnolvdMaarel/http://gbb.eldoc.ub.rug.nl/root/2002/JBiotechnolvdMaarel/


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lng t ngun tinh bt, ngi ta thng cho thm enzyme amylase vo. Enzymeamylase s tham gia phn gii tinh bt to thnh ng, gip cho qu trnh chuynha tinh bt tt hn.

2. ng Dng Ca Enzyme Amylase Trong Dc Phm.Ngun cung cp enzyme amylase l vi khunBacillus subtilis vi u im l bnhn trong mi trng acid ca d dy so vi diastase (amylase) ly t ng vt vvi nm. Amylase c s dng phi hp vi coenzyme A, cytocrom C, ATP,carboxylase ch thuc iu tr bnh tim mch, bnh thn kinh. Mt khc amylase

phi hp vi enzyme thy phn cha bnh thiu enzyme ng tiu ha. Trongc th, amylase l hormon tuyn ty ngoi tit, c tc dng chng ph n sau chnthng hoc sau m. iu tr triu chng phn ng vim km nhim khun ngh hp trn hoc di.-amylase dng trong Neopeptine vin nang c ly t ngun vi khun Bacillus

subtilis.Sn phm cha men tiu ha dnh cho tr s sinh v tr em, kch thch tiu ha,chng suy dinh dng v cc sn phm cng loi :

3. ng Dng Ca Enzyme Amylase Trong Cng Nghip DtTrong cng nghip dt, ngi ta thng s dng enzym amylase ca vi khun ty tinh bt v lm cho vi mm.Ngi ta thng s dng lng ch phm amylasekhang 0,3-0,6 g/l dung dch v thi gian x l 5-15 pht nhit 90 oC.4. ng Dng Ca Enzyme Amylase Trong Vic Ty Mu Giy.Enzyme -amylase c kh nng thc y quy trnh kh mc tt.Chng b gy ccmch tinh bt trn mt giy, ko theo s lm long cc phn t mang mu bm trn, to thun li cho qu trnh tuyn ni kh mc. (7)

Nguyn liu: giy photocopy, giy vn phng loi, giy in bng mc laser mt mtc gia keo tinh bt.Quy trnh x l: bao gm cc bc ngm v x l giy loi bng enzyme -amylaseti nhit thng, x l c ha nhit: nh ti c pha trn ha cht v gia nhit, ti iu kin nhit , pha long v tuyn ni, ra v c c, thnh phm.Ha cht s dng: enzyme -amylase, NaOH cng nghip, Na2SiO3, H2O2,cht hot tnh b mt, cht to bt, du bo.Hiu qu so vi mu i chng khng s dng enzyme -amylase: thi gian gim30%, mc dng cc loi ha cht gim 10 - 35% ty theo tng loi, gim nhiu nhtl mc dng NaOH (gim 35%), trng ca bt sau kh mc tng 2,7 - 3,0% ISO,


MINIGADIN TRYMO CALCINOL-RB


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di t tng 1,5%, chu gp (i ln) tng 14% v chu x tng 28%. trng cui cng t ti a 87,0% ISO.5. ng Dng Ca Enzyme Amylase Trong Sn Xut Bia:

Trong cng ngh sn xut bia truyn thng, cc nc phng Ty ch yu s dngenzym amylase ca malt thy phn tinh bt trong malt, sau n giai onru ha bi nm men Saccharomyces sp.C s khoa hc ca vic s dng amylase ca malt ch, khi i mch chuyn ttrng thi ht sang trng thi ny mm (malt), enzym amylase s c tng hp vkhi enzym ny s thy phn tinh bt c trong ht to ra nng lng v vt chtcho s to thnh mm.

Nh vy vic ng ha tinh bt trong ht nh enzym ca chnh n. Khi ht chtng hp ra lng enzym amylase va phn hy lng tinh bt c trong ht.

Nh th cn rt nhiu mm i mch sn xut bia qui m ln, dn n chi phcao cho sn xut v sn phm. khc phc iu ny, trong qu trnh ln men to bia th nh sn xut khng sdng hon tan 100% nguyn liu l malt i mch m c s pha trn theo mtcng thc no thay th malt v cn b sung ngun tinh bt cho qu trnh lnmen. L do l mt phn to hng v cho bia, mu sc, cn ph hp chongi tiu dng v mt phn l lm gim gi thnh cho sn phm bia nhng vn gic c trng cho bia. Chnh v iu ny, cc nh sn xut bia quan tm n vics dng ch phm enzym amylase cung cp cho qu trnh thy phn tinh bt.6. ng Dng Ca Enzyme Amylase Trong Sn Xut Cn:

Qu trnh sn xut cn tri qua hai giai an:giai an ng ha v giai an ruha. Giai an ng ha, ngi ta bt buc phi s dng enzym amylase (khngth s dng phng php thy phn tinh bt bng acid). Giai an ru ha, nhnm men Saccharomycescerevisiae, cng c th xem y l mt qu trnh p dngenzym. Qa trnh ru ha l qu trnh ht sc phc tp, tri qua rt nhiu giai onchuyn ha t ng thnh cn nh s tham gia ca nhiu enzym khc nhau. imkhc vi enzym amylase l ch cc enzym tham gia qu trnh ru ha nm trongt bo nm men. Vic iu khin cc qu trnh chuyn ha bi enzym trong t bothc cht l qu trnh trao i cht ca nm men trong mi trng cha ng.7. ng Dng Ca Enzyme Amylase Trong Sn Xut HFCS:

HFCS (High Fructose Corn Syrup) l mt nhm bt k ca siro bp m tri qua qutrnh enzyme lm tng hm lng fructose v sau c pha trn vi siro bpsch (100% glucose) to thnh dng sn phm cn thit.

Nguyn nhn chnh l:o Nguyn liu r M, n ph thuc vo gi c ca bp v ng.o Sn phn d dng pha trn v di chuyn bi v n dng lng.

Cc dng ph bin ca HFCS:o HFCS 90o HFCS 55o HFCS 42



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Qu trnh enzyme lm thay i 100% glucose siro bp thnh HFCS 90 theocc bc sau:

o Tinh bt bp c x l bng -amylase sn xut chui ngngn hn gi l oligosaccharides.

o Glucoseamylase ph v nhng chui ng sn xut ng nglucose.

o Xylose isomerase bin i glucose to ra hn hp c khong 42%fructose v 50- 52% glucose vi mt vi loi ng c ha trn.

o Hn hp 42 - 43% fructose glucose phi chu qua mt bc sc klng ni m fructose s c lm giu n xp x 90%.

IV. KT LUN :Enzyme amylase l mt trong nhng enzyme c quan tm nghin cu sm

v nhiu nht hin nay. Cc ng dng ca enzyme trong nhiu lnh vc khc nhaungy cng c quan tm nghin cu. Ngoi nhng ng dng tiu biu c trnh

by trn, enzyme amylase cn c ng dng trong sn xut gim, bt ngt, bnhko, nc tri cy Ngy nay ngi ta ang nghin cu cc ngun amylase c chtlng cao v c th sn xut theo quy m cng nghip ng thi khc phc nhnghn ch ca cc phng php sn xut to enzyme amylase nng sut cao. Vit

Nam bc u c nhiu nghin cu ng dng enzyme amylase trong ch bin

nng sn, thc phm, nht l trong lnh vc sn xut bia, ru, ch bin tinh bt(Vin cng nghip thc phm, Vin cng ngh sinh hc cng ngh thc phm,i hc Bch khoa H Ni). (8)



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TI LIU THAM KHO :

(1). Nguyn c Lng v mt s tc gi, Cng ngh enzym, NXB i hc qucgia Tp.HCM, 2004(2). Nguyn c Lng, Vi sinh vt cng nghip ( Cng ngh vi sinh Tp 2), NXBi hc quc gia Tp.HCM, 2006(3). Lng c Phm, Nm men cng nghip, NXB Khoa hc v k thut H Ni,2006(4). Nguyn Th Hin ( ch bin), Cng ngh sn xut m chnh v cc sn phm lnmen c truyn, NXB Khoa hc v k thut H Ni, 2006

(5). Nguyn Tin Thng, Gao trnh cng ngh enzym, Trng i hc K thutcng ngh Tp.HCM, 2008(6).www.bio-link.org/sharing_day/fungalamylase.pdf(7).http://giaoan.violet.vn/present/showprint/entry_id/1758225

(8).http://www.ykhoanet.com/duoc/duocpham/tracuu/DM000248.htm

(9).http://gbb.eldoc.ub.rug.nl/root/2002/JBiotechnolvdMaarel/

(10).http://www.khoahoc.com.vn

http://www.bio-link.org/sharing_day/fungalamylase.pdfhttp://giaoan.violet.vn/present/showprint/entry_id/1758225http://www.ykhoanet.com/duoc/duocpham/tracuu/DM000248.htmhttp://gbb.eldoc.ub.rug.nl/root/2002/JBiotechnolvdMaarel/http://www.khoahoc.com.vn/http://www.bio-link.org/sharing_day/fungalamylase.pdfhttp://giaoan.violet.vn/present/showprint/entry_id/1758225http://www.ykhoanet.com/duoc/duocpham/tracuu/DM000248.htmhttp://gbb.eldoc.ub.rug.nl/root/2002/JBiotechnolvdMaarel/http://www.khoahoc.com.vn/

Enzyme Amylase Phương Pháp Sản Xuất Và Ứng Dụng

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Transcript of Enzyme Amylase Phương Pháp Sản Xuất Và Ứng Dụng