通过免疫控制口蹄疫 - UMN College Vet Med...Antibody and Protection of Foot-and-Mouth...

FOOT AND MOUTH DISEASE CONTROL BY VACCINATION

通过免疫控制口蹄疫

Dr. Shih-Ping Chen 陈世平博士Agriculture Technology Research Institute

农业科技研究院

Agricultural Technology Research Institute

Outlines 纲要

FMD in Taiwan 台湾的口蹄疫 Serum neutralizing titers and protection 血清中和抗体滴度和保护

Vaccine releasing test in Taiwan 台湾疫苗上市试验 Field trials of vaccinated pigs 免疫猪的生产试验 Selection of right vaccine strain 选择合适的疫苗株 Duration of Immunity and Vaccination schedule 免疫持续时间和免疫程序

Discussion 讨论


FMD in Taiwan台湾的口蹄疫

In Taiwan 97, a species-specific adaptation to pigs 1997年台湾地区，种特异性表现在猪上

Overt clinical signs in pigs 猪有明显的临床症状 Vaccination has been successfully used for controlling

FMD 免疫已成功用于控制口蹄疫 Been recognized as FMD free with vaccination before 2008 在2008年之前由于免疫被确认为无口蹄疫

Effective methods of differentiating vaccinated pigs from infected 鉴别免疫猪和被感染猪的有效方法


Humoral responses are more important than the cytotoxic reactions in protective immune response against FMD 对口蹄疫保护性免疫反应，体液免疫反应比细胞毒性反应更重要

FMD SN titers do correlate with the protection in cattle 口蹄疫血清中和滴度与在牛身上的保护作用相关

Determine the protected SN titer in pigs 确定猪的保护的血清中和抗体滴度

Relationship between serum neutralizing titers and protection血清中和滴度和保护力之间的关系


Relationship between serum neutralizing titers and protection from FMD in pigs after oil emulsion

vaccination 油乳剂疫苗接种后的血清中和抗体滴度和对口蹄疫保护之间的关系

Black L et al. 1984.

血清抗体滴度

（lo

g 10

)


Correlations Between Serum Neutralization Antibody and Protection of Foot-and-Mouth Disease In Pigs Lin et al. 2004. 血清中和抗体和对猪的口蹄

保护的相关性 248 pigs were vaccinated with double oil emulsion vaccines

of by IM once at 8-week old (74 pigs) challenged at 4 weeks post vaccination or twice at 8- and 12-week old (174 pigs) challenged at 20 WPV 248头猪用双油乳剂疫苗在第8周肌肉注射免疫一次（74头），免疫后4周攻毒，或第8、12 周免疫两次，免疫后20周攻毒

Homogeneous strain was used for challenge (O/TAW strain) 用同源毒株攻毒（O/TAW 株）

Subcutaneous injection with 105 TCID50 of FMDV into the heel bulbs of the right forefoot 在右前脚根部用105 TCID50 口蹄疫病毒皮下注射


Correlation between SN antibody titers and protection against FMD virus challenge. Pigs in panel A were challenged at 4 WPV and in panel B were challenged at 20 WPV. Filled circles, protection; Open circles, no protection.

血清中和抗体滴度和对攻毒保护的相关性。A组 – 免疫后4周攻毒， B组 – 免疫后20周攻毒。实心圆，保护; 空心圆，没有保护。


SN titers did not correlate well with the protection, but pigs with SN titers 1:64 did provide a better protection for pigs of the 4 WPV。

血清中和抗体滴度与保护力没有好的相关性，但中和抗体滴度1:64的猪对4周后攻毒的猪提供了较好的保护。


Batch tests in swine with FMD vaccines: Viral Neutralization Test for batch releasing test

猪口蹄疫疫苗批次实验：批次上市的病毒中和试验


Vaccine releasing test in Taiwan台湾的疫苗上市试验

FMD vaccine quality is critical for the control of FMD and in Taiwan this quality was evaluated by SN titers instead of by the challenge test 口蹄疫疫苗质量对口蹄疫控制至关重要，在台湾，质量是通过中和抗体滴定来评估，而不是攻毒试验

In vaccine test, the geometric mean SN antibody titers of 5 pigs were determined after 3 or 4 weeks of vaccination and vaccines producing titers 1:32 was considered to pass the test 在疫苗试验中，5头猪的血清中和抗体的几何平均值在免疫后3或4周来评估，达到滴度1:32 的疫苗为通过测试


Durat ion o f immuni ty and MDA interference trials: different swine vaccination studies in Taiwan after 1997 FMD outbreaks

免疫持续时间和MDA干扰实验： 1997年口蹄疫疫情爆发后台湾不同的免疫研究


Vaccination study after 1997 outbreak of FMD 1997爆发口蹄疫后的免疫研究

Seventy pigs, 8-week-old, on farm L were randomly allocated into one of seven groups L场70 头8周龄的猪随机分为七组

Vaccinated with one of seven commercially available monovalent FMD serotype O1 Manisa, O 4174 or O1 Campos oil-emulsified vaccines (Vaccines A–H—see Table 1) 用7种单价油乳剂商品苗，口蹄疫血清型O1马尼萨， O4174或O1坎波斯免疫（疫苗A-H-见表1）

All pigs received the first FMD vaccination at 8 weeks of age and a secondary vaccination at 12 weeks of age 所有猪在第8周龄免疫第一针，12周龄第二针

Serum samples were collected from pigs at 8, 12, 16 and 23 weeks of age 第8，12，16和23周龄采集血清样本

Measurement of neutralizing antibody titers and NSP antibodies. 测定中和抗体效价和NSP抗体。


Seven commercially available FMD vaccines used in the vaccination experiments

在免疫实验中使用的七种商品口蹄疫疫苗

Vaccine designation Origin of vaccine Virus strain

A Argentina O1 Campos

B Argentina O1 Campos

C Argentina O1 Campos

D England O 4174

E Germany O1 Manisa

F Turkey O1 Manisa

G Holland O1 Manisa

疫苗编号疫苗来源毒株


Primary vaccination Boost

两次疫苗接种

滴度

初级免疫加强

接种后周数


单次免疫

平均Log2滴度免疫后周数


NSP抑

制%

阳性

%

疫苗

第一次免疫前

第一次免疫前

第一次免疫后15周

第一次免疫后15周


Appropriate vaccine strains related to OTaiwan/97 were determined based on the evaluation of r1 values 根据评估r1值确定与OTaiwan/97相关的免疫毒株

The r1 values for O1 Manisa, O 4174 and O1 Campos were 1, 1 and 0.7,respectively， O1 Manisa, O 4174 和 O1 Campos 的r1值分别为 1, 1 和 0.7,

However, vaccines E, F and G formulated with O1 Manisa induced lower neutralizing titers than those vaccines with O1 Campos and O 4174. 然而，用O1 Manisa 生产的E、F、和G 比用O1 Campos 和 O 4174生产的疫苗产生较低的中和抗体滴度

Other factors which can cause a difference in vaccine efficacy include antigen mass contained in vaccines, vaccine production procedures and antigen adjuvant interactions其它导致疫苗效果差异的因素包括疫苗中抗原量，疫苗生产过程及抗原与佐剂的相互作用


免疫后监测母猪体中滴度

滴度

每月监控


Immune response of sows after vaccination with heterologous strains 母猪接种异源毒株后的免疫反应

免疫时间

中和

滴度


Comparison of neutralizing titers in pigs vaccinated with homologous and

heterologous vaccines

比较用同源和异源毒株疫苗免疫的中和抗体滴度


中和滴度

免疫时间


Effect of maternal Ab on the antibody titers of pigs at 4 weeks after vaccination

免疫4周后母抗对猪的抗体滴度的作用


仔豬移行抗體 log10

Matermal Ab母抗


Matermal Ab母抗


仔豬移行抗體 log10

Matermal Ab母抗

168 pigs free from FMD randomly divided into three groups 168头没有口蹄疫的猪被随机分成三组

A-imported from England A组-从英国进口B-imported from Argentina B组-从阿根廷进口C-imported from Russia C组-从俄罗斯进口

Field trials 生产试验


第一次免疫后周数

中和滴度


For all three vaccines, geometric mean neutralizing antibody titers peaked by 2 weeks after booster vaccination and had declined by 12 weeks post vaccination but were still higher at that time than titers 4 weeks after primary vaccination.

对于所有三种疫苗，中和抗体滴度几何平均值加

强免疫2周后达到最高，免疫12周后下降，但仍高于初免4周后的滴度。

Profile of Neutralizing Titer with Double doses

两针免疫中和抗体滴度的情况

第一次接种后周数

中和滴度


Sequential neutralizing antibody titers in pigs vaccinated with double dose of vaccines B and C at 12, 16 and 20 weeks of age and tested for antibodies at 12, 16, 20 and 24 weeks of age. 在12，16和20周龄猪中接种两针疫苗B和C的连续中和抗体滴度，检测在12，16，20和24周龄的抗体.

For both vaccines geometric mean neutralizing antibody titers increased 4 weeks after each vaccination. 对于这两种疫苗，中和抗体滴度几何平均值每次接种后4周增加.


Selection of right vaccine strain 选择合适的疫苗株

Antigenic diversity (serotypes) 抗原多样性（血清型）

Select vaccine strains from actual field isolates or traditional vaccine strains ? 选择猪场分离出的毒株或传统疫苗株?

Ø FMD naive country: 口蹄疫阴性的国家seronegative pigs: 血清阴性猪：

Single vaccination of seronegative pigs protects 3-6 months (data depending on vaccine strains ) 血清阴性猪单次接种可保护3-6个月（根据疫苗株的数据）

• Sow vaccination with Heterologous/homologous vaccine strains 母猪接种异源/同源疫苗株

Heterologous vaccine strains: 3 months 异源的疫苗株：3个月 Homologous vaccine strain: 6 months 同源疫苗株：6个月

Duration of Immunity and Vaccination schedule 免疫持续时间免疫程序


Ø FMD infected / vaccinated country: 口蹄疫病毒感染/免疫的国家：

seropositive pigs: 血清阳性猪：

Multiple vaccinated sows with natural infection have high antibody titers自然感染并多次免疫的母猪有较高的抗体滴度

Variable MDA titers in piglets 仔猪中MDA滴度有差异

Interference of heterologous vaccines with high MDA titers有高MDA滴度的异源疫苗的干扰

Ø Two vaccination in fatteners: 育肥猪两次免疫：

1st vaccination at 8 weeks and 2nd vaccination at 12 weeks with homologous vaccine 用同源毒株疫苗在8周和12周两次免疫

Delay of vaccination time when using heterologous vaccines 用异源毒株疫苗时，推迟免疫时间

Discussion 讨论

通过免疫控制口蹄疫 - UMN College Vet Med...Antibody and Protection of Foot-and-Mouth...

Documents

Transcript of 通过免疫控制口蹄疫 - UMN College Vet Med...Antibody and Protection of Foot-and-Mouth...