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EARLY PERIPHERAL NERVE REGENERATION IN THE ARTERY-INCLUDING TUBULATION OF THE RAT. MASAAKI KOSAKA" MASAMITSU HAMAGUCHI", TOSHIHARU --- ASAI*, TATSUYA TOKUNO", ATSUSHI CHIBA, AND SHIKO CHICHIBU. -- Department of Physiology, Kinki University School of Medicine, Ohno-Higashi, Osaka-Sayama, Osaka 589 , Japan.

Although the nerve entubritirin method (ilrbulation) is a!, interesting t,cb nique for repairing transected nerve, the results of nerve regeneration have been restricted to a 15 mm long gap. In order to elucidate the importance of the microvascular environment for better nerve regeneration, an artery-in- cluding tubulation(AIT) was examined and compared to the conventional "empty" tubulation with electrophysiological and microscopic study. In twenty-nine male Sprague-Dawley rats, following anesthesia with intraperitoneal injection _-_ of Nembutal, the femoral nerve was transected, and a 5 mm gap tubulation group was made (as the control). For AIT, an accompanying femoral artery was inserted within that tubing. By 5,7,9 and 11 days following surgery, both better nerve regeneration and intraneural vascular reconstruction occurred within the AIT lumen than in the control. The rate of neurite elongation was greater than 2 mm/day. Microvascular supply may be an important factor in the development of the regenerating nerve.

DISTRIBUTION AND FIBER CONNECTION 0~ NERVE CR~H FAVOR RECEPTOR (NOF-R) CONTAINING PERIPHERAL NERVOUS SYSTEM. MIKAKO IKEDA’ SADAO SHIOSAKA’*, YASUHIDE LEE’, HIROSHI HATANAKA’* AND ML\SAYA TOHYAMA3*, Department of Neuroanatomy,~iomedicaI Research Center, Osaka University Medical Schooll, Institute of Protein Research Osaka University’, and Department of Anatomy, Osaka University Medical School’, 4-3-57 Nakanoshima, Kitaku, Osaka 530, Japan.

The localization of NOF-R in the peripheral nervous system was examined by means of an inmunocytochemical technique using MC-192 IgC, monoclonal ant ibody against PC-12 derived NGF-R. NOF-R was found in the medium or large-sized neurons in the dorsal root ganglion, the trigeminal ganglion, the superior and inferior gang1 ion and superior cervical gang1 ion. In the superior and inferior gang1 ion, some of the NGF-R-positive neurons were also stained by CGRP or SP antisera. With inject ion of fluorogold, retrograde tracer into the solitary tract nucleus and trigeminal spinal tract nucleus area, and insnunostaining of the s ame sect ions using NGF-R antiserum, a number of tracer-labeled and imnunostained neurons were detected in both ganglia. Thus, the results indicate that NOF-R containing neurons in the ganglia project to the medulla oblongata, and terminate in the solitary tract nucleus (from inferior ganglion) anf trigeminal spinal tract nucleus (from superior ganglion), while some coexist with CORP or SP.

11. EEG and evoked potentials

SOURCE LOCALIZATION OF EVENT-RELATED POTENTIALS IN NAN BY MEANS OF THE DIPOLE TRACING METHOD. YOSHIO NAKAJIMA, Department of Physiology, School of Medicine, Chiba University, 1-8-1, Inohana, Chiba 280, Japan.

Experiments were performed on young and healthy volunteers after obtaining informed consent. Electroencephalic activity was recorded with twenty-one surface electrodes placed on the scalp according to the international lo-20 system. Bed or green LBDs were illuminated at random intervals and the sequence of the red or green LED illumination was randomly changed at the probability of 50/50 or 70/30% (red/green). The subject was asked to press a button or count when the green LED was turned on, and ignore pressing the button or counting when the red LED was on. Brain potentials of 128-256 times, triggered by green or red illumination, or button pressing before and after the instruction, were averaged to obtain the event-related potentials (EP). The dipole tracing method developed by us (J. Neuro- sci. Meth., 1987) was applied to the EPs to estimate the current sources. Infrequent stimuli (green LED) elicited positive potentials at a latency of about 300 ms in a series of experiments of counting. The source of the positive potentials was estimated deep in the brain. task,

In experiments of the button pressing similar positive potentials as those obtained in the counting task were

obtained when the red LED was on. The estimated location of the positive potentials was also distributed in a deep part of the brain. These locations are discussed.