热门信号通路集锦 -...

热门信号通路集锦• Autophagy

• Apoptosis

• Histone modi�cation

• JAK/STAT

• MAPK

• mTOR

• NF-κB

• Oxidative Stress Pathway

• P53

• PI3-Kinase/Akt

• TGF-β

• Toll-like Receptor (TLR)

• Wnt

目录第一部分：Autophagy Signaling............................................................................................................ 3

第二部分：Apoptosis Signaling............................................................................................................... 7

第三部分：Histone modi�cation.......................................................................................................... 13

第四部分：JAK/STAT Signaling.............................................................................................................. 22

第五部分：MAPK Signaling................................................................................................................... 27

第六部分：mTOR Signaling.................................................................................................................... 30

第七部分：NF-κB Signaling.................................................................................................................. 33

第八部分：Oxidative Stress Pathway.................................................................................................. 38

第九部分：P53 Signaling........................................................................................................................ 40

第十部分：PI3-Kinase/Akt Signaling................................................................................................... 43

第十一部分：TGF-β Signaling...............................................................................................................48

第十二部分：Toll-like Receptor (TLR) Signaling............................................................................... 51

第十三部分：Wnt Signaling...................................................................................................................53

Autophagy Signaling细胞自噬(autophagy) 是近年来生命科学领域的一个研究热点，是真核生物中进化保守的对细胞内物质进

行周转的重要过程。该过程中一些损坏的蛋白或细胞器被双层膜结构的自噬小泡包裹后，送入溶酶体(动物）或液泡（酵母和植物）中进行降解并得以循环利用。

细胞自噬可能由很多因素诱导产生，如饥饿、生长因子缺乏、微生物感染、细胞器损伤、蛋白质折叠错

误或聚集、DNA损伤、放疗、化疗等等。这些信号可以通过mTOR的抑制或AMPK活化来引起细胞自噬。细

胞自噬发生后会形成自噬泡（phagophore），ULK1，2/Atg13复合物促进Atg复合物进入到自噬泡膜上，

ATG14L/Vps34蛋白复合物发生三磷酸化反应，从而募集更多的Atg复合物进入到膜上。接着自噬泡通过泛

素化途径使膜生长延长而形成自噬小体。在自噬小体成熟期间，蛋白酶ATG4降解其脂双层膜上的LC3-II (LC3-PE)。最后自噬体和溶酶体融合，其中的大分子和氨基酸等组分可以重新被细胞利用。

目前普遍认为自噬是一种防御和应激调控机制。细胞可以通过自噬和溶酶体，消除、降解和消化受损、

变性、衰老和失去功能的细胞、细胞器和变性蛋白等。为细胞重建、再生和修复提供必需原料，实现细

胞再循环和再利用。另一方面，自噬在机体的免疫、感染、炎症、肿瘤、心血管疾病、神经退行性疾病

的发病中也具有十分重要的作用。

3

Autophagy Regulators Panel自噬调节剂套装，目录号189488

Leupeptin,Hemisulfate,SyntheticAMPK Inhibitor,Compound CAutophagy Inhibitor,3-MAAutophagy Inducer,STF-62247Ba�lomycin A1,Streptomyces griseusDynamin Inhibitor I,DynasoreDAPK InhibitorHDAC Inhibitor XXIII,Tubastatin AmTOR Inhibitor III,PP242Necrostatin-1Nigericin,Sodium Salt,Streptomyces hygroscopicusNocodazolePi�thrin-μPI-103RapamycinPIKfyve InhibitorSMER28Tamoxifen,4-Hydroxy-,(Z)ThapsigarginCaspase Inhibitor I,Z-VAD-fmkTunicamycin,Streptomyces lysosuper�cusWiskostaitinbpV(phen)DMSO

autophagic proteolytic inhibitorautophagy inhibitorhVps34,autophagosome formation blockerautophagy inducer,target TGN and enhance autolysosomal acidi�cationvacuolar ATPase inhibitor,autophagasome maturation blockervesicle �ssion inhibitor,autophagy blockerautophagy inhibitorHDAC6 selective autophagy inhibitor,autophagosome-lysosome fusion blockermTORC1/2 inhibitor,autophagy inducerNecroptosis blockerionophore,alter intracellular PH,-blocks lysosomal protein degradation

microtubule disruptor,autophagosome-lysosome fusion blockerautophagy inducer,target iHSP70 and inhibit lysosomal functionDNA-PK,PI3-K,and mTORC1/2 inhibitor,autophagasome inducermTOR inhibitor,autophagy inducerslows down the autophagic breakdown of proteinsmTOR-independent autophagy inducerautophagy inducer-estrogen antagonist,increase ceramide & Beclin 1 levelssarco/endoplasmic reticulum Ca2+ATPase inhibitor,ER stress and autophagy inducerapoptotic inhibitorProtein N-glycosylation inhibitor,ER stress and autophagy inducerN-WASP inhibitor,autophagosomes intracelluar trafficking blockerPTEN inhibitor,autophagy suppressor

自噬研究试剂盒Product Catalogue No.

Akt/mTOR/S6K Pathway Explorer Antibody MiniPack

15-104

FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (100 tests)

FCCH100171

FlowCellect™ Autophagy Reagent Pack FCCF200097

FlowCellect™ LC3-GFP Reporter Autophagy Assay Kit (CHO)

FCCH100170

FlowCellect™ LC3-GFP Reporter Autophagy Assay Kit (U2OS)

FCCH100181

Autophagy Detection Reagent Pack CF200097

Autophagy Detection Reagent Pack CF200097

LC3-II Enrichment Kit (Western Blot) 17-10232

LentiBrite™ GFP-LC3-II Enrichment Kit (Flow Cytometry)

17-10230

LentiBrite ™ GFP-LC3 Lentiviral Biosensor 17-10193

LentiBrite ™ GFP-LC3 Control Mutant Lentiviral Biosensor

17-10189

LentiBrite ™ RFP-LC3 Lentiviral Biosensor 17-10143

LentiBrite ™ RFP-LC3 Control Mutant Lentiviral Biosensor

17-10188

mTOR phosphorylation Pathway Explorer Antibody MiniPack

15-105

108976-10mg171260-1mg189490-50mg189497-10mg196000-10μg324410-10mg324788-10mg382187-1mg475988-5mg480065-5mg481990-5mg

487928-10mg506155-10mg528100-1mg553210-1mg524611-5mg573121-10mg579002-5mg586005-1mg627610-1mg654380-10mg681525-1mg203695-10mgKP31817-15ml

组分靶标目录号

*以上组分可按目录号单独购买

4

Search for your antibody at: www.merckmillipore.com/antibodies

参考文献:

Increased susceptibility of cytoplasmic over nuclear

polyglutamine aggregates to autophagic

degradation. Iwata, Atsushi, et al. Proc. Natl. Acad.

Sci. U.S.A., 102: 13135-40 (2005) 2005

The p75 neurotrophin receptor can induce autophagy

and death of cerebellar Purkinje neurons.

Florez-McClure, Maria L, et al. J. Neurosci., 24:

4498-509 (2004) 2004

Role of the Apg12 conjugation system in

mammalian autophagy. Mizushima, Noboru, et al.

Int. J. Biochem. Cell Biol., 35: 553-61 (2003) 2003

参考文献:

Increased susceptibility of cytoplasmic over nuclear

polyglutamine aggregates to autophagic

degradation. Iwata, Atsushi, et al. Proc. Natl. Acad.

Sci. U.S.A., 102: 13135-40 (2005) 2005

Role of the Apg12 conjugation system in

mammalian autophagy. Mizushima, Noboru, et al.

Int. J. Biochem. Cell Biol., 35: 553-61 (2003) 2003

Product Catalogue No.

Akt Inhibitor X 124020

AMPK Activator VI, RSVA314 171272

AMPK Inhibitor, Compound C 171260

Autophagy Inducer, STF-62247 189497

Autophagy Inhibitor, 3-MA 189490

Autophagy Regulators Panel 189488-1EA

Ba�lomycin A1, Streptomyces griseus 196000

bpV(phen) 203695

Cathepsin L Inhibitor, CAA0225 219502

DAPK Inhibitor 324788

Dynamin Inhibitor I, Dynasore 324410

ICMT Inhibitor 420350

Leupeptin, Hemisulfate, Synthetic 108976

mTOR Inhibitor III, PP242 475988

Necrostatin-1 480065

Niclosamide 481909

Nigericin, Sodium Salt, Streptomyces hygroscopicus

481990

Nocodazole 487928

PI-103 528100

Pi�thrin-µ 506155

PIKfyve Inhibitor 524611

Rapamycin 553210

SMER28 573121

Spautin-1 567569

Thapsigargin 586005

Wiskostatin 681525

自噬研究抑制剂Product Catalogue No.

Anti-Abl (c-, v-, Bcr-), clone 24-21 MABT203

Anti-APG10 AB15408

Anti-APG5 AB15404

Anti-APG5 AB15404P

Anti-ATG13, clone 2H4.2 MABC46

Anti-ATG16L1 ABC25

Anti-ATG18 (WIPI-2), clone 2A2 MABC91

Anti-ATG3 AB2953

Anti-ATG4A ABC29

Anti-ATG4B ABC32

Anti-ATG4C ABC21

Anti-ATG4D ABC22

Anti-ATG5 ABC14

Anti-ATG5, clone 177.19 MAB2605

Anti-ATG7 AB10511

Anti-ATG9 ABC23

Anti-Beclin-1 (C-term), clone EPR1733Y, rabbit

MABN16

Anti-Beclin-1 AB15417

Anti-Beclin-1, clone 9A1.1 MABC34

Anti-DRAM1 MABC62

Anti-DRAM2 MABC64

Anti-GABARAP AB15415

Anti-GABARAPL1 AB15278

Anti-GATE-16 ABC24

Anti-LC3A (N-term), clone EP1528Y,

rabbit monoclonalMABC177

Anti-LC3A, clone EPR1754, rabbit monoclonal

MABC175

Anti-LC3A/B (N-term), clone EP1983Y, rabbit monoclonal

MABC176

Anti-phospho-ULK1 (Ser555) ABC124

Anti-phospho-ULK1/ATG1 (Ser758) ABC112

自噬研究抗体

Anti-Phospho-p62/SQSTM1(Ser403),Clone 4F6 monoclonal antibody MABC186

Anti-phospho-ULK1(Ser467)，clone15D12.3 MABC307

5

LentiBrite™ Lentiviral Biosensors — 实时监测细胞自噬，自噬小体精确定位

用 GFP-LC3 慢病毒颗粒转染 HeLa 细胞。（A）用完全培养基培养细胞；（B）在含有溶酶体抑制剂的 Earle’s Balanced Salt Solution (EBSS) 中饥饿培养。

利用LentiBrite™ GFP- and RFP-LC3，即使是在很难转染的细

胞中，也可以实时监测细胞的自噬情况，并对形成的自噬

小体进行精确定位。

产品优势：

使用方便，重复性好 – 预包被，GFP- & RFP-标签蛋白

复合物

实验稳定 – 长期、稳定的荧光表达

转染高效 - 更高的转染效率，对细胞无干扰，低免疫原性结果易说明 - LC3突变的慢病毒颗粒，含有转位缺陷蛋

白LC3-G120A，用于对照实验。

•

• • •

货号

17-10193

17-10188

17-10189

17-10143

产品描述

LentiBrite™ GFP-LC3 Lentiviral Biosensor

LentiBrite™ RFP-LC3 Control Mutant

LentiBrite™ GFP-LC3 Control Mutant

LentiBrite™ RFP-LC3 Lentiviral Biosensor

细胞自噬研究创新技术及工具

LC3-II富集前后，用Western blot 对其进行检测 (Catalogue No. 17-10232)

LC3-II Enrichment Kit

— 细胞自噬标志蛋白 LC3-II检测新方法

(WB & Flow Cytometry)

LC3-II 富集试剂盒 (Western Blot)和 LentiBrite™ LC3-II 富集试

剂盒(Flow-Cytometry)使用了选择性渗透的方法，保留了自

噬体上的LC3-II，去除了细胞质中的LC3-I的干扰，从而可

以灵敏准确地对自噬体定量检测。

货号

17-10230

17-10232

产品描述

LentiBrite™ GFP-LC3-II Enrichment Kit

LC3-II Enrichment Kit (Western Blot)

6

Apoptosis Signaling对凋亡的逃逸能力，是绝大多数癌症的特征。许多蛋白，包括细胞表面受体、配体、蛋白酶和有丝分裂

组分，调节着细胞的生存与死亡的精细平衡。在癌症中，这些感应分子或作用分子的突变，会破坏这个

平衡，从而逃脱凋亡的调控。

细胞凋亡的不同时期具有特征性的生化及形态学上的改变，可以通过多种实验方法和技术检测细胞凋

亡。由于细胞凋亡常常具有复杂的机制，在特定的实验体系中建议使用两种及以上的不同方法进行验

证。默克密理博可以为您提供凋亡研究的全面解决方案及实验技术（如质膜改变，线粒体改变，caspase活化，DNA断裂等）。

7

}

Granzyme

Perf

orin Granzyme A

Granzyme B

Phosphatidylserine Phosphatidylserine

Caspase 9

Procaspase 3 Caspase 3

NME1

SET Complex

p50 p65

p50 p65

IκB

IKK

Akt

Smac/Diablo

HtrA2/Omi tBid

Bid

Caspase 8

Ca2+

p50

FLIPcIAPBcl2

p53

SurvivinBidBak/BaxNoxa/Puma

p65

MDM2

p70S6K

Akt

AktmTOR

Cell Shrinkage

PIP2

PIP3

PDK1

p90RSK

ERK1/2

MEK

Raf

Ras

PI3K

Procaspase 3, 6, 7

IAPp35CrmA

PerforinGranzyme

Caspase 3, 6, 7

Nur

77/T

R3 cJunATF2

JNK/SAPK

CeramideSphingo-myelinase

Elk1

Fos

CREB

elF-4B

AIF W

OX1

Endo

nucl

ease

G

His

tone

H1.

2

NUCLEUS

APOPTOSOME

Growth Factor

TNFα

TNFR

FasLFAS

Fas Decoy

Receptor

BaxBax

PP

P

PP

P

P

P

P

P

PP

PP

PP

P

P

P

PP

PP

Bax Bad

Bcl2Bcl2

ICAD

CAD

CAD

ICAD

CAD

TRAF2RIP

PARP

Bax

TRAD

Procaspase 8

Puma Noxa

Cytotoxic T Cell

1

2

3

4

4

5

78

9

ATP

Apaf-1

Cyt c

∆ Ψm

Late Stage: DNA fragmentation

Nuclear collapse

Formation of apoptotic bodies

Phagocytosis by macrophages

Early Stage:

Loss of mitochondrial membrane potential (∆Ψm)

Mid Stage: Activation of caspases (3,6,7)

Poly-ADP-Ribose Polymerase (PARP) cleavage

Cell shrinkage

Activation of nucleases

Cytochrome c & ATP release

Activation of caspase 8 and 9

12

3

4

56

7

8

910

11

12

Translocation of phosphatidyl-serine to the outer leaf of plasma membrane

抗体

Name Description

Anti-AIF Antibody, internal domain

Anti-Apaf-1 Antibody, CT

Anti-Arginase 1 Antibody

Anti-Bad Antibody, clone BYC001

Anti-BAFF receptor Antibody

Anti-Bak Antibody, NT

Anti-Bax (NT) Antibody

Anti-Bax Antibody

Anti-Bcl2 Antibody, clone 100

Anti-Bcl-X Antibody, clone 2H12

Anti-Beclin Antibody1, clone 4E10.1

Anti-Bid Antibody

Anti-Bim Antibody, internal epitope,

pan-Bim isoforms

Anti-Blys/TALL-1/BAFF/THANK Antibody

Anti-Bmf Antibody

Anti-Calpain Antibody, small subunit of

µ- or m-Calpains (Calpain I or II), clone

P1

Anti-Caspase 1 Antibody


Anti-Caspase 10 Antibody, clone 16C5.1

Anti-Caspase 12 Antibody, clone 14F7

Anti-Caspase 2 Antibody, clone 10C6


Anti-Caspase 3 Antibody,

active (cleaved) form


AB16501

AB16503

ABC897

05-605

ABC337

06-536

ABC11

AB2915

05-729

MAB4625

MABC139

AB1735

AB17003

07-167

ABC348

MAB3083

AB1871

06-503-I

MABC192

MABC555

MAB3501

06-735

AB3623

AB1879

Key Applications

Immunohistochemistry

(Paraf�n);Western Blotting

Western Blotting

Western Blotting;

Immunohistochemistry;

Immunocytochemistry

Immunoprecipitation;Western Blotting

Western Blotting;


Immunocytochemistry;Immunohistoch

emistry;Immunoprecipitation;Western

Blotting

Western

Blotting;Immunoprecipitation;Immuno

cytochemistry;Immunohistochemistry

Western Blotting;

Immunohistochemistry (Paraf�n)

Flow Cytometry; Western Blotting


Flow Cytometry;

Immuno�uorescence;Immunoprecipita

tion;WesternBlotting;


Western Blotting

Western

Blotting;Immunoprecipitation;


Western Blotting

Western Blotting

Western

Blotting;Immunohistochemistry

ELISA;Western Blotting;

Immunocytochemistry;


Immuno�uorescence;Immunoprecipita

tion;Western Blotting

Western Blotting;


Western Blotting;


Western Blotting;



ELISA;Flow Cytometry;


Western Blotting

Immuno�uorescence;Immunohistoche

mistry;Immunohistochemistry

(Paraf�n);Western Blotting

Western Blotting

Species Reactivity

Human;Mouse;Rat

Human;Mouse;Rat

Human;Mouse

Human;Mouse

Human;Mouse;Rat

Human;Rat;Mouse

Human;Bovine;Feline;

Chimpanzee;Canine

Mouse

Human;Canine;Feline

Human;Mouse;Pig;Rat

Human;Mouse;Rat

Human;Mouse;Rat;Rabbit

Human;Mouse;Rat

Human

Human

Bovine;Human

Human;Mouse;Rat

Human

Human;Rat;Mouse

Mouse;Human;Rat

Canine;Human;Mouse;Monkey

Human;Mouse;Rat

Human;Mouse;Rat

Bovine;Human;Mouse;

Monkey;Rat

Qty/Pk

100 µg

100 µg

100 µL

200 µg

100 µg

200 µg

200 µg

100 µL

100 µg

50 µg

100 µg

100 µL

100 µg

100 µg

100 µg

100 µL

50 µg

100 µg

100 µg

100 µg

100 µg

200 µg

50 µg

50 µg

8

抗体

Name Description

Anti-Caspase 9 Antibody, clone 2-23

Anti-Caspase-10 Antibody

Anti-Caspase-14 Antibody

Anti-Caspase-8 (active form p18 subunit)

Antibody, clone 2B12.1

Anti-Cathepsin D Antibody

Anti-COX2 , clone EPR468(2), Rabbit

Monoclonal Antibody

Anti-COX4 Antibody

Anti-c-Rel (NFκB) Antibody

Anti-Cytochrome C Antibody

Anti-Death-associated protein 1

Antibody

Anti-DR4/TRAIL-R1 Antibody

Anti-FADD Antibody, clone 1F7

Anti-FAF1 Antibody

Anti-Fas Antibody (human, activating),

clone CH11

Anti-Fas Ligand Antibody

Anti-Fractin Antibody, CT

Anti-GADD153/DDIT3/CHOP Antibody

Anti-IRE1 Antibody

Anti-Kallikrein 2/KLK2 Antibody, clone

4F2.3

Anti-Kallikrein-1/KLK1 Antibody, clone

11C7.1

Anti-Kallikrein-13/KLK13 Antibody,

clone 33.1

Anti-Kallikrein-3 (KLK3) (CT) Antibody

Anti-Kallikrein-5 Antibody/KLK5

Anti-Mitofusin-2 (Mfn2) Antibody

Anti-p53 Upregulated Modulator of

Apoptosis (PUMA) Antibody

Anti-PARP Cleaved Form Antibody

Anti-PARP Poly ADP-ribose Polymerase-1

Antibody

Anti-Phospho- Bad (Ser75) Antibody

Anti-phospho Beclin Antibody (Thr119)

MAB4709

ABC343

ABC841

MAB10754

06-114

MABC754

AB10526

09-040

AB3547

ABC259

06-744

05-486

ABC346

05-201

AB16982

AB3150

ABC955

ABC241

MABC563

MABC552

MABC971

AB10428

ABC470

ABC42

ABC158

AB3620

AB16661

AB10423

ABC118

Key Applications

Immunoprecipitation;Western


(Paraf�n)

Western Blotting

Western Blotting;


Western Blotting;

Immunocytochemistry

Western Blotting;

Immunocytochemistry

Western Blotting

Western Blotting;


ELISA;Electrophoretic Mobility Shift

Assay;Western Blotting

Western Blotting;

Immunocytochemistry

Western


Western Blotting

Western Blotting

Western Blotting;


Flow Cytometry; Western Blotting


ELISA;Western Blotting

Western Blotting;



Western Blotting

Western Blotting

Western Blotting;


Western Blotting;



ELISA

Western Blotting

Western Blotting;


Western Blotting

Western Blotting;


Western Blotting


Western Blotting

Western Blotting;Peptide Inhibition

Assay

Species Reactivity

Human;Mouse

Human

Human

Human

Human

Human

Human

Human

Canine;Human;Rat;Rabbit

Mouse;Human

Human;Mouse;Rat

Human;Mouse

Human;Rat

Human

Human;Mouse;Rat

Human;Mouse;Rat

Human

Human

Human

Human

Human

Human;Mouse;Rat

Human;Mouse

Human;Rat;Mouse

Human

Human

Bovine;Human;Mouse;Rat

Mouse;Rat

Human

Qty/Pk

100 µg

100 µg

100 µg

200 µg

100 µg

100 µL

100 µg

100 µg

100 µg

100 µg

200 µg

100 µg

100 µg

50 µg

50 µg

100 µL

100 µL

100 µg

100 µg

100 μg

100 µg

100 µg

100 µg

100 µg

200 µg

50 µg

100 µL

100 µg

100 µg

11

9

抗体

Name Description

Anti-phospho Mitofusin2 Antibody

(Ser442)

Anti-phospho TRADD (Ser215) Antibody

Anti-Phospho-Bad (Ser134) Antibody

Anti-phospho-Bcl-2 (Ser70) Antibody,

clone 69-10C-2-10C-18, rabbit

monoclonal

Anti-Phospho-Bid (Ser65) Antibody

Anti-phospho-IRE1 (Ser724), clone

EPR5253, Rabbit Monoclonal Antibody

Anti-Poly ADP-ribose Antibody, clone

10H

Anti-RIAP-3 (XIAP) Antibody

Anti-RIP3 Antibody, clone 6E6.2

Anti-Smac/Diablo Antibody, clone 3A9.1

Anti-Survivin Antibody

Anti-Survivin Antibody

Anti-TNFα Receptor Antibody, clone

13F9.1

Anti-TRADD Antibody, clone 1G6.1

Anti-TRAF1 Antibody, clone 1F3

Anti-Traf2 Antibody

Anti-TRAF4 Antibody, clone 5MLN-2H1

Anti-TRAIL Antibody, clone N2B2

Anti-TRAIL/CD253 Antibody, clone 6D12.2

Anti-VDAC Antibody

ABC963

ABC461

AB10422

05-843R

AB2994

MABC742

MABC547

07-753

MABC28

MABC174

AB3610

AB3611

MABC615

MABC988

MABC260

ABC47

MABC985

MABC12

MABC147

AB10527

Key Applications


Assay


Assay

Western Blotting

Western


Flow Cytometry

Western Blotting

Western Blotting;Flow Cytometry

Western Blotting;

Immunoprecipitation;Immuno�uoresc

ence

Western Blotting

Western


Western Blotting;


Western



Western Blotting

Western Blotting;


Western Blotting;


Western Blotting;

Immunohistochemistry;Flow Cytometry

Western Blotting;

Immunocytochemistry

Western Blotting;


Immunocytochemistry

Flow Cytometry

Flow Cytometry;


Western Blotting

Species Reactivity

Human

Human

Human

Human

Human

Human

Human

Human;Rat

Human;Rat

Human

Human

Mouse

Human;Mouse

Human;Rat

Monkey;Human

Human;Mouse;Rhesus

Macaque

Human;Mouse

Mouse

Human

Human;Mouse;Rat;Bovine;

Horse;Rhesus;Macaque;

Chimpanzee;Pig

Qty/Pk

100 µg

100 µL

100 µg

100 µL

100 µg

100 µL

50 µg

200 µg

200 µL

100 µg

100 µL

100 µg

100 μg

100 μg

100 µL

100 µg

100 µL

100 µg

100 µg

100 µg

10

抑制剂

货号产品描述

Apoptosis Inhibitor II, NS3694

APT1 Inhibitor, palmostatin B

BAX CHANNEL BLOCKER

Bax Channel Blocker II, iMAC2

Bax-Inhibiting Peptide, V5

Bax-Inhibiting Peptide, Negative Control

Bcl-xL BH4(4-23) (Human), Cell-Permeable

Caspase-2 Inhibitor I

Caspase-1 Inhibitor VI

Caspase-1 Inhibitor VI

Caspase-3 Inhibitor III

Caspase-8 Inhibitor II




Caspase-8 Inhibitor I, Cell-Permeable

Caspase-3/7 Inhibitor I

Caspase-3/7 Inhibitor II

InSolution(TM) Caspase-8 Inhibitor II

InSolution(TM) Caspase-9 Inhibitor I

Caspase Inhibitor VI

Caspase Inhibitor VI

InSolution(TM) Caspase Inhibitor VI

Caspase-3 Inhibitor VII

b-Catenin/Tcf Inhibitor III, iCRT3

CFTR Inhibitor III, OXO-172


InSolution Caspase-3 Inhibitor I, Cell-P

Histone Methyltransferase EZH2 Inhibitor, DZNep


178494-10MGCN

178501-5MGCN

196805-5MGCN

196806-10MGCN

196810-5MGCN

196811-5MGCN

197217-1MGCN

218744-1MGCN

218746-1MGCN

218746-250UGCN

218750-1MGCN

218759-1MGCN

218759-250UGCN

218761-1MGCN

218761-250UGCN

218773-1MGCN

218826-1MGCN

218832-1MGCN

218840-250UGCN

218841-250UGCN

219007-1MGCN

219007-250UGCN

219011-1MGCN

219012-10MGCN

219332-10MGCN

219672-10MGCN

235420-1MGCN

235427-1MGCN

252790-2MGCN

264155-1MGCN

货号产品描述


Fas/FasL Antagonist, Kp7-6

Foxo1 Inhibitor, AS1842856

GSK-3 beta Inhibitor XXIV

Granzyme B Inhibitor I

Histone Acetyl Transferase Inhibitor VII, CTK7A

Heat Shock Factor 1 Inhibitor, KRIBB11




Caspase-1 Inhibitor IV

HIF-Hydroxylase Inhibitor, DMOG

HIF-PHD Inhibitor II, JNJ-42041935

IL-6/Stat3 Signaling Inhibitor, Cpd 6a

IRE1 Inhibitor I, STF-083010

Kir2.1 Inhibitor, ML133

Necrostatin-1

Necrostatin-1, Inactive Control

MLKL Inhibitor, Necrosulfonamide

NOX Inhibitor III, VAS2870

Omi/HtrA2 Protease Inhibitor, Ucf-101

Pi�thrin-a

Pi�thrin-a

Pi�thrin-a, Cyclic-

PICK1 PDZ Domain Inhibitor, FSC231

InSolution Q-VD-OPh, Non-O-methylated

ROS Inhibitor, YCG063

Caspase Inhibitor I

Caspase Inhibitor I

264155-250UGCN

341291-25MGCN

344355-10MGCN

361567-5MGCN

368050-1MGCN

382115-10MGCN

385570-10MGCN

400010-1MGCN

400010-5MGCN

400012-5MGCN

400015-1MGCN

400091-50MGCN

400093-10MGCN

407625-5MGCN

412510-10MGCN

422689-5MGCN

480065-5MGCN

480066-5MGCN

480073-25MGCN

492000-10MGCN

496150-10MGCN

506132-10MGCN

506132-5MGCN

506134-10MGCN

529531-10MGCN

551476-1MGCN

557354-5MGCN

627610-1MGCN

627610-5MGCN

Milliplex kit

Early Apoptosis-7 Plex(Cat.No.48-669MAG)AB2

Analyte Total Phosphorylated

Akt/PKB

BAD

Bcl-2

Active Caspase 8

Active Caspase 9

JNK/SAPK1

p53

(Ser473)

(Ser112)

(Ser70)

(Thr183/Tyr185)

(Ser46)

H,M,R

H

H

H

H

H,M,R

H

Human Late Apoptosis-3 Plex(Cat.No. 48-670MAG)AB1


Active Caspase 3

Cleaved PARP

GAPDH

H,M

H

H

11

4500-0450/4500-0455

PF032-1EA

PF036-1EA

4500-0250

APT142/APT242

475866-1KIT

QIA87-1KIT

CBA077-1KIT

FCCH100110

H0XPSMAG-16K

RM0XPSMAG-17K

QIA23-1EA

FCCS025108

48-669MAG

QIA90/QIA92-1KIT

218790-1KIT

APT131/APT165

QIA70-1KITQIA91/QIA93

17-198

17-367

APT423/APT403

4500-0540

QIA108-1KIT

QIA113-1KITAPT428/APT408

4500-0550QIA71-1KIT

APT129/APT171

APT139/APT173

218824-1KIT

QIA72-1KIT

APT172

QIA115-1KITAPT429/APT409

4500-0560

S7100/S7101

S7110/S7111

QIA33-1EA

QIA39-1EA

4500-0121

APT151

AM41-1EA

QIA20-1EA

Guava Nexin Reagent

ANNEXIN V FITC APOPTOSISDETECTION KIT

ANNEXIN V BIOTIN APOPTOSISDETECTION KIT

Guava® MitochodrialDepolorization Kit

MITOLIGHT MITOCHNDRAPPTSIS DET KIT

MITOCAPTURE APOPTOSISDETECTION KIT

CYTOCHROME C RELEASEAPOPTOSIS ASSAY KIT

INNOCYTE CYTOCHROME CRELEASE KIT

FlowCellect Cytochrome C KitMILLIPLEX MAP Human

Oxidative Phosphorylation(OXPHOS) Magnetic Bead Panel

MILLIPLEX MAP Rat/MouseOxidative Phosphorylation

(OXPHOS) Magnetic Bead Panel

BCL-2 ELISA KIT

FlowCellect™ Bcl-2 ActivationDual Detection Kit

MILLIPLEX MAP Human EarlyApoptosis Magnetic Bead

6-Plex Kit

CASPASE DETECTION KITCASPASE-1 ASSAY KIT,

COLORIMETRICCaspase 3 Colorimetric Activity

Assay Kit, DEVDCASPASE-3 ACTIVITY ASSAYCASPASE-3 DETECTION KIT

Caspase 3 Activity Detection KitCaspase 3/7 Assay Kit

(Ac-DEVD-AMC Substrate)CaspaTag Caspase 3,7 In Situ

Assay Kit, FluoresceinGuava® Caspase 3/7 FAM Kit

Caspase-7 Immunoassay/ActivityKit Fluorometric

CASPASE-8 DETECTION KITCASPATAG CASPASE 8 ASSAY

Guava® Caspase 8 FAM KitCASPASE-8 ACTIVITY ASSAY

Caspase 8 Colorimetric ActivityAssay Kit, IETD

Caspase 9 Colorimetric ActivityAssay Kit, LEHD

CASPASE-9 ASSAY KIT,COLORIMETRIC

CASPASE-9 ACTIVITY ASSAYCaspase 9 Fluorometric Activity

Assay Kit, LEHDCASPASE-9 DETECTION KIT

CASPATAG CASPASE 9 ASSAYGuava® Caspase 9 FAM Kit

ApopTag® Peroxidase In SituApoptosis Detection Kit

ApopTag® Fluorescein In SituApoptosis Detection Kit

TDT FRAGEL TM DNA FRAGMENT.DETECT. KIT

FLUORESCEIN FRAGEL DNA FRAG.DETECT. KIT

Guava TUNEL Kit (100 Tests)APOPTOTIC DNA LADDER

DETECTION KITSuicide Track™ DNA Ladder

Isolation KitCell Death Detection (Nuclear

Matrix Protein) ELISA Kit

默克密理博凋亡试剂盒选择指南

早期凋亡

中期凋亡

凋亡时期货号品名检测对象样本类型检测方法/所需设备产品优势备注

默克密理博为您提供凋亡研究的整体解决方案，包括凋亡检测试剂盒，诱导剂，抑制剂/激活剂，抗体，蛋白和酶等等。产品详情请参考：www.merckmillipore.com

* 红色标记的为现货产品

反应次数不同

晚期凋亡

磷脂酰丝氨酸

线粒体膜电位

细胞色素C

线粒体氧化磷酸化

Bcl-2

Active Caspase 8 (Asp384), Active Caspase 9(Asp315), Akt (Ser473), BAD (Ser112), Bcl-2(Ser70), JNK (Thr183/Tyr185), p53 (Ser46)

总Caspase

Caspase 1

Caspase 3

Caspase 3/7

Caspase 7

Caspase 8

Caspase 9

DNA片段化

NMP

活细胞

活细胞

活细胞

活细胞

活细胞

活细胞

细胞或组织裂解液

活细胞

活细胞



血清,血浆,细胞培养上

清,细胞或组织裂解液

活细胞

血清,血浆,细胞培养上

清,细胞或组织裂解液

活细胞




活细胞



活细胞

活细胞


活细胞

活细胞活细胞


细胞裂解液

细胞裂解液



细胞裂解液

活细胞

活细胞

活细胞

固定细胞或组织切片

活细胞,固定细胞或组织

切片

固定细胞或组织切片

活细胞,固定细胞或组织

切片

活细胞

贴壁或悬浮细胞

贴壁或悬浮细胞

细胞培养上清

流式细胞仪

流式细胞仪,荧光显微镜


流式细胞仪



免疫印迹


流式细胞仪

Luminex仪器

Luminex仪器

酶标仪

流式细胞仪

Luminex仪器


酶标仪

酶标仪

荧光酶标仪


荧光酶标仪

荧光酶标仪


流式细胞仪

荧光酶标仪



流式细胞仪

荧光酶标仪

酶标仪

酶标仪

酶标仪

荧光酶标仪

荧光酶标仪



流式细胞仪

光学显微镜

荧光显微镜,流式细胞仪

光学显微镜

荧光显微镜,流式细胞仪

流式细胞仪

DNA胶

DNA胶

酶标仪

一步法,离心次数少,减少假阳性

有快速结合试剂,离心次数少,减少假阳性

实验简单,易操作

细胞凋亡新的检测方法

细胞凋亡新的检测方法

产品选择多,包括总的caspase检测和caspase家族成员分别检测。实验方法选择多,可以用活细胞进行流式或显微镜检测,也可用酶标仪对细胞或组织裂解液进行检测。

产品选择多，包括总的caspase检测和caspase家族成员分别检测。实验方法选择多，可以用活细胞进行流式或显微镜检测，也可用酶标仪对细胞或组织裂解液进行检测。

高性价比，多选择性

试剂盒组分齐全,有阴阳性对照,灵敏度高

实验次数不同

标记荧光颜色不同

实验次数不同



实验次数不同

实验次数不同


实验次数不同

S7100是核心试剂,S7101是全组分

S7100是核心试剂,S7101是全组分

直接取细胞上清检测,细胞还可以用于其他检测

12

Histone Modi�cations最常见和研究最透彻的组蛋白修饰包括甲基化、乙酰化、磷酸化和泛素化。组蛋白修饰广泛调控着DNA转录、修复、重组和复

制，并可改变染色质结构。

乙酰化

甲基化

磷酸化

泛素化

瓜氨酸化

组蛋白乙酰化酶（HAT）和去乙酰化酶（HDAC）是基因表达与功能调控的重要因素。转录激活复合物所包含的HAT可

将组蛋白含有的赖氨酸乙酰化并打开染色质结构以启动转录。HDAC则可移除乙酰基团，从而抑制基因表达。

特定组蛋白残基的甲基化是常染色质和转录处于激活状态的重要标志，而其他的甲基化事件则标志着异染色质和转

录的抑制。组蛋白甲基化可被位点特异性地组蛋白去甲基化酶去除，如LSD1，UTX，JMJD等酶家族。组蛋白的甲基

化与去甲基化作用从时间和空间上调控着基因表达，尤其是在胚胎发育过程中。

组蛋白磷酸化通常发生在有丝分裂时染色体缩合期和DNA损伤导致的细胞凋亡过程中。然而，特定组蛋白位点的磷

酸化与早期基因感应信号相关，这表明组蛋白磷酸化可能对位点或细胞环境信号产生应答，介导染色质结构的打开

与关闭。

泛素化作用参与某些组氨酸甲基化过程，而泛素化作用需要泛素激活酶（E1）、泛素结合酶（E2）和泛素-蛋白连接

酶（E3）的参与。我们为您提供包括特异性识别泛素连接和组蛋白修饰残基的抗体在内的各种泛素化相关产品。

瓜氨酸是一种经特殊修饰的精氨酸，它可能与类风湿关节炎和多发性硬化等疾病的发生相关。10%的组氨酸带有瓜

氨酸修饰，说明瓜氨酸在基因调控中起作用。默克密理博为您提供针对瓜氨酸修饰的组蛋白H4的特异性抗体及用于

检测这种修饰的抗体和方法。

C

120110

100

90

80

N

1020

30

40

50

N

C

10

20

30

40

50

60

80

90

100

CP

PMe

MeMe

Me

Ac

Ub

70

80

90

100

110 120

Ub

N

10

2030

40

P

P

P

P

P

P

P

Me

Ac

MeAc

Ac

Ac

Me

MeAc

Ac

50

Me Me

Me

Me

H2B(P62807*)

H2A(P0C0S8*)

H3(P68431*)

H4(P62805*)

N

140

CP

PP

P

120130

H2A.X(P16104*)

C

130

120

110

100

90

Me2

P

P

P

P

P

P

MeMeMe

MeMe

Me

Ac

Ac

Ac

Ac

Ac

Ac

Ac

Ac

Me

MeAc

Ac

Ac

AcMe

Me

AcAc

Ac

Ac

AcAc

Ac

Ac

Me

Me

AcAc

Ac

Ac

Ub

P

P

P

P MeMe

Ac

AcAc AcAc

Ac

Me

Me

10

20

30

40

80

Me2

Me2

Me2

Me3

AcAc

Me2

Me3

Me3

Me2 Me2

Me2

Me2

Me2

Me3

Me2

Me3

Me2Me2

MeAc

Me2

Me3

MeAc

Me2

Me3

Acetylation

Methylation

Dimethylation

Trimethylation

PhosphorylationP

Me

Me2

Me3

Ac Ub

*

Ubiquitination

Amino acids buried in nucleosome core

Solvent-accessible amino acids

UniprotKB Accession Number

Download an interactive version of this poster at: www.millipore.com/histonemodapp

13

DescriptionName Key Applications Species Reactivity Qty/Pk

未修饰组蛋白抗体

MAB3864

05-457

05-629

07-146

06-131907-627AB1002209-862

07-59407-371

AB162305-1352

07-680

06-755

05-1341

05-499

07-690

04-928

05-928

09-838

ABE154

07-108

07-59604-858

05-858R

05-858

MAB3422

MABE71

MAB1276

Anti-DNA/Histone H1 Antibody

Anti-Histone H1 Antibody, clone AE-4Anti-Histone H1o/H5 Antibody, clone 3H9Anti-Histone H2A (acidic patch) AntibodyAnti-Histone H2A.Bbd AntibodyAnti-Histone H2A.X AntibodyAnti-Histone H2A.X AntibodyAnti-Histone H2A.Z Antibody

Anti-Histone H2A.Z AntibodyAnti-Histone H2B Antibody

Anti-Histone H2B AntibodyAnti-Histone H2B Antibody, clone 5HH2-2A8Anti-Histone H2B Antibody, testis variantAnti-Histone H3 Antibody

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301

Anti-Histone H3 Antibody, clone 6.6.2Anti-Histone H3 Antibody, CT, panAnti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonalAnti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonalAnti-Histone H3.3 Antibody

Anti-Histone H3.1/H3.2 Antibody

Anti-Histone H4 Antibody

Anti-Histone H4 (citrulline 3) Antibody Anti-Histone H4 Antibody, pan, rabbit monoclonalAnti-Histone H4 Antibody, pan, clone 62-10C-2, rabbit monoclonalAnti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonalAnti-Histone Antibody, clone H11-4Anti-Histone Antibody, Pan, clone F152.C25.WJJAnti-Nuclei & Chromosomes Antibody, histone H1 protein, clone 1415-1

ELISA;Flow Cytometry;Immunocytochemistry;ImmunohistochemistryELISA;Immunocytochemistry;Western Blotting

Western Blotting;Immunocytochemistry

Western Blotting;Chromatin Immunoprecipitation (ChIP)Western BlottingPeptide Inhibition Assay;Western BlottingWestern Blotting;Flow CytometryWestern Blotting

Western BlottingWestern Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;ImmunohistochemistryWestern Blotting;Immunocytochemistry

Western Blotting

Western Blotting;Immunocytochemistry;Immunoprecipitation;Chromatin Immunoprecipitation (ChIP)Western Blotting;Immunocytochemistry;ELISA;Immunoprecipitation;Flow Cytometry;Dot BlotWestern Blotting

Western Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;Immunocytochemistry;Dot Blot;Chromatin Immunoprecipitation (ChIP)Western Blotting;ChIP-seq;Chromatin Immunoprecipitation (ChIP)Western Blotting

Western Blotting;ImmunohistochemistryWestern Blotting;Multiplexing;Chromatin Immunoprecipitation (ChIP)Western Blotting

Western Blotting;Multiplexing;ChIP-seq; ChIP

Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;ELISA

ELISA;Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)

All

Human;Bovine;Green PlantsBovine;Human;Mouse;Rat

Mouse;Rat;Human;Chicken;Yeast (S.cerevisiae)Human;MouseHumanHuman;Mouse;Rat;BovineChicken;Human;Zebrafish;Rabbit;Rat;Xenopus;MouseHumanChicken;Xenopus;Human;Yeast (S. cerevisiae)HumanMouse;Human;Rat

Human;Mouse

Human;Rat;Mouse

Human;Vertebrates

Vertebrates;Green Plants;HumanChicken;Human;Mouse;Rat;Yeast (S. cerevisiae)Chicken;Human;Mouse;Rat;Yeast (S. cerevisiae)Human;Chicken;Rat;Mouse;Yeast (S. cerevisiae)Human;Mouse

Mouse;Human

Mouse;Human;Bovine;Avian;Chicken;XenopusHuman;VertebratesChicken;Human;Vertebrates;Yeast (S. cerevisiae)Human

Human;Chicken;Vertebrates;Yeast (S. cerevisiae)Mammals;Human;Bovine;Rat;Xenopus;Opossum;HamsterHuman;Bovine

Human;Rat

100 µg

200 µg

100 µg

200 µL

100 µg200 µL100 µL100 µg

100 µL200 µg

100 µL100 µL

100 µL

100 µg

100 µg

200 µg

100 µL

100 µL

100 µL

100 µg

100 µg

200 µL

200 µL100 µL

100 µL

100 µL

100 µg

100 µg

100 µL

14


乙酰化组蛋白抗体

05-1334

ABE18

05-1232

05-1355

07-376

07-290

07-289

AB10020

07-373

07-564

07-382

06-599

07-539

07-353

06-911

07-354

07-355

07-360

07-540

07-750

07-352

06-942

07-593

16-658

06-866

06-598

07-595

04-119

07-329

07-327

04-118

Anti-Acetyl Histone H3 (Lys27) Antibody, clone CMA309Anti-acetyl Histone H3 (Lys9) AntibodyAnti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10Anti-acetyl-Histone H2A AntibodyAnti-acetyl-Histone H2A (Lys5) AntibodyAnti-acetyl-Histone H2A (Lys9) AntibodyAnti-acetyl-Histone H2A.X (Lys5) AntibodyAnti-acetyl-Histone H2B AntibodyAnti-acetyl-Histone H2B (Lys120) AntibodyAnti-acetyl-Histone H2B (Lys5) AntibodyAnti-acetyl-Histone H3 Antibody

Anti-acetyl-Histone H3 (Lys 4) AntibodyAnti-acetyl-Histone H3 (Lys14) AntibodyAnti-acetyl-Histone H3 (Lys14) AntibodyAnti-acetyl-Histone H3 (Lys18) AntibodyAnti-acetyl-Histone H3 (Lys23) AntibodyAnti-acetyl-Histone H3 (Lys27) AntibodyAnti-acetyl-Histone H3 (Lys36) AntibodyAnti-acetyl-Histone H3 (Lys79) AntibodyAnti-acetyl-Histone H3 (Lys9) AntibodyAnti-acetyl-Histone H3 (Lys9) AntibodyAnti-acetyl-Histone H3 (Lys9/18) AntibodyAnti-acetyl-Histone H3 Antibody, Agarose ConjugateAnti-acetyl-Histone H4 Antibody

Anti-acetyl-Histone H4 Antibody

Anti-acetyl-Histone H4 (Lys12) AntibodyAnti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonalAnti-acetyl-Histone H4 (Lys16) AntibodyAnti-acetyl-Histone H4 (Lys5) AntibodyAnti-acetyl-Histone H4 (Lys5) Antibody, rabbit monoclonal

Human;Vertebrates

Human;Mouse;Chicken

Human

Human;Mouse;Rat

Human

Human

Human;Vertebrates

Human

Human

Human

Human

Human;Mouse;Rat

Human

Human;Yeast (S. cerevisiae)Human;Eukaryote

Human;Vertebrates;Yeast (S. cerevisiae)Human;Vertebrates;Yeast (S. cerevisiae)Human;Vertebrates;Yeast (S. cerevisiae)Human

Human

Human;Vertebrates;Yeast (S. cerevisiae)Human;Mouse;Rat

Bovine;Human;Mouse;Yeast (S. cerevisiae)Human;Mouse;Tetrahymena

Human;Tetrahymena;EukaryoteTetrahymena;Human

Human;Yeast (S. cerevisiae)Human;Vertebrates

Human;Mouse;Rat

Human

Human;Vertebrates

100 µg

100 µg

100 µg

100 µL

100 µL

100 µL

200 µL

100 µg

100 µg

100 µL

100 µg

100 µg

100 µL

100 µL

200 µg

100 µL

100 µL

100 µL

100 µL

100 µL

100 µL

200 µg

200 µL

200 µL

200 µL

200 µg

100 µL

100 µL

100 µL

100 µL

100 µL

ELISA;Immunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting;Dot Blot;Multiplexing; ChIP;ChIP-seqWestern Blotting;Peptide Inhibition Assay


Western Blotting

Western Blotting

Western Blotting

Western Blotting;Multiplexing

Western Blotting

Dot Blot;Western Blotting;Peptide Inhibition AssayWestern Blotting;Dot Blot;ImmunoprecipitationWestern Blotting;Immunocytochemistry;ChIP-seq; ChIPMultiplexing;Western Blotting;Peptide Inhibition Assay;ChIP-seq;Dot BlotWestern Blotting;ChIP-seq;Dot Blot;Multiplexing; ChIPImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting;Dot Blot; ChIP;ChIP-seq

Western Blotting;Dot Blot; ChIP

Western Blotting;ChIP-seq; ChIP;Dot Blot


Western Blotting;ELISA

Western Blotting;Multiplexing;ChIP-seq; ChIP

Western Blotting; ChIP;Dot Blot;Flow Cytometry;ChIP-seqWestern Blotting;Multiplexing; ChIP

Immunoprecipitation

Western Blotting;Immunocytochemistry; ChIP

Immunocytochemistry;Immunoprecipitation;Western Blotting; ChIPDot Blot;Western Blotting;ChIP-seq


Western Blotting;Multiplexing;Peptide Inhibition Assay;Dot Blot;ChIP-seq; ChIPWestern Blotting;Dot Blot;Immunocytochemistry;ChIP-seq; ChIPWestern Blotting

15

超过50%的文章使用该抗体进行实验

参考文献:

Baf60c drives glycolytic metabolism in the muscle

and improves systemic glucose homeostasis

through Deptor-mediated Akt activation.

Meng, Zhuo-Xian, et al. Nat. Med., 19: 640-5 (2013)

2013 GATA2 Is a Critical Transactivator for the

Human IL1RL1/ST2 Promoter in Mast Cells/

Basophils: OPPOSING ROLES FOR GATA2 and

GATA1 IN HUMAN IL1RL1/ST2 GENE EXPRESSION.

Yosuke Baba,Keiko Maeda,Takuya Yashiro,Eisuke

Inage,Kazumi Kasakura,Ryuyo Suzuki,Fran

Niyonsaba,Mutsuko Hara,Atsushi Tanabe,Hideoki

Ogawa,Ko Okumura,Yoshikazu Ohtsuka,Toshiaki

Shimizu,Chiharu Nishiyama,François Niyonsaba

The Journal of biological chemistry 287 2012


参考文献:

Acetylation limits 53BP1 association with damaged

chromatin to promote homologous recombination.

Tang, Jiangbo, et al.

Nat. Struct. Mol. Biol., 20: 317-25 (2013) 2013

Chromatin Modulation at the FLO11 Promoter of

Saccharomyces cerevisiae by HDAC and Swi/Snf

Complexes. Ramón R Barrales,Philipp Korber,Juan

Jimenez,José I Ibeas Genetics 191 2012



甲基化组蛋白抗体

07-328

04-557

Anti-acetyl-Histone H4 (Lys8) AntibodyAnti-acetyl-Histone H4 Antibody, pan (Lys 5,8,12), rabbit monoclonal

Human;Yeast (S. cerevisiae)Human

100 µL

100 µL

Immunoprecipitation;Western Blotting;Dot Blot;ChIP-seqWestern Blotting

04-791

ABE10

07-436

06-1416

ABE101

07-450

07-395

05-1248

07-448

07-548

07-748

07-1570

04-735

07-608

MABE19

ABE250

05-1338

05-1249

07-1584

07-214

04-077

05-808

07-585

Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonalAnti-monomethyl Histone H3 (Arg8) AntibodyAnti-monomethyl-Histone H3 (Lys4) Antibody

Anti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306Anti-monomethyl-Histone H3 (Lys27) AntibodyAnti-monomethyl-Histone H3 (Lys36) AntibodyAnti-monomethyl-Histone H4 (Lys20) AntibodyAnti-monomethyl Histone H4 (Lys20) AntibodyAnti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonalAnti-dimethyl Histone H3 (Lys23) AntibodyAnti-dimethyl Histone H3 (Lys27) Antibody, clone 8H2Anti-dimethyl Histone H3 (Lys4) AntibodyAnti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303

Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307

Anti-dimethyl Histone H4 (Lys20) AntibodyAnti-dimethyl-Histone H3 (Arg17) AntibodyAnti-dimethyl-Histone H3 (Arg17) Antibody, rabbit monoclonalAnti-dimethyl-Histone H3 (Arg2) Antibody, rabbit monoclonalAnti-dimethyl-Histone H3 (Arg2) Antibody

Human;Vertebrates

Human

Human;Vertebrates

Human

Human

Human;Mouse;Chicken

Human

Human

Human;Vertebrates

Vertebrates

Human

Human

Human

Human

Human

Human;Mouse;Rat;Chicken

Human;Vertebrates

Human;Vertebrates

Human

Human;Vertebrates

Human;Vertebrates

Human

Chicken;Human

100 µL

200 µL

200 µg

100 µg

100 µg

100 µg

100 µL

100 µg

200 µg

100 µL

200 µg

100 µg

100 µl

100 µL

100 µg

100 µL

100 µg

100 µg

100 µg

100 µL

100 µL

100 µL

200 µL

Western Blotting;Peptide Inhibition Assay;Multiplexing; ChIP

Western Blotting;Peptide Inhibition Assay

Immunocytochemistry;Immunofluorescence;Peptide Inhibition Assay;Western Blotting;ChIP-seq;Dot BlotWestern Blotting;Immunocytochemistry;Peptide Inhibition AssayWestern Blotting;Dot BlotImmunocytochemistry;Peptide Inhibition Assay;Dot Blot;Immunocytochemistry;Peptide Inhibition Assay;Western BlottingWestern Blotting


Dot Blot;Immunocytochemistry;Western Blotting;ChIP-seqDot Blot;Immunofluorescence;Western Blotting;MultiplexingWestern Blotting;Multiplexing;ImmunocytochemistryWestern Blotting;Dot Blot; ChIP;Peptide Inhibition AssayWestern Blotting;Multiplexing

Western Blotting;Dot Blot;Multiplexing

Western Blotting;ELISA;Immunocytochemistry

Western Blotting;Immunocytochemistry;Dot Blot; ChIP;ChIP-seqWestern Blotting;Immunoprecipitation;Dot Blot;Immunocytochemistry;ELISA;Multiplexing; ChIPWestern Blotting;ELISA;Immunocytochemistry;Immunoprecipitation;Multiplexing; ChIPWestern Blotting

ELISA;Immunocytochemistry;Western Blotting

Western Blotting

Dot Blot;Western Blotting;Peptide Inhibition Assay;MultiplexingWestern Blotting;Peptide Inhibition Assay

16


04-808

07-215

07-452

04-821

07-274

07-030

04-790

05-684

04-835

05-768R07-212

07-441

16-187

04-768

07-947

07-367

05-672

07-528

05-809

ABE44

05-1951

05-1339

07-992

07-952

05-1250

04-809

07-449

Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonalAnti-dimethyl-Histone H3 (Arg26) AntibodyAnti-dimethyl-Histone H3 (Lys27) AntibodyAnti-dimethyl-Histone H3 (Lys27) Antibody, clone 614M, rabbit monoclonalAnti-dimethyl-Histone H3 (Lys36) Antibody

Anti-dimethyl-Histone H3 (Lys4) Antibody

Anti-dimethyl-Histone H3 (Lys4) Antibody, clone AW30, rabbit monoclonalAnti-dimethyl-Histone H3 (Lys4) Antibody, clone RR302Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, rabbit monoclonalAnti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal

Anti-dimethyl-Histone H3 (Lys9) AntibodyAnti-dimethyl-Histone H3 (Lys9) Antibody, biotin conjugateAnti-dimethyl-Histone H3 (Lys9) Antibody, clone MC554, rabbit monoclonalAnti-dimethyl-Histone H4 (Arg3) Symmetric AntibodyAnti-dimethyl-Histone H4 (Lys20) AntibodyAnti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 AntibodyAnti-trimethyl (Lys9)-phospho (Ser10)-Histone H3 Antibody, rabbit monoclonalAnti-trimethyl Histone H3 (Lys27) Antibody

Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304Anti-trimethyl Histone H3 (Lys4/Lys9) AntibodyAnti-trimethyl Histone H3 (Lys79) AntibodyAnti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308Anti-trimethyl(Lys9)-phospho(Ser10)-Histone H3 Antibody, rabbit monoclonalAnti-trimethyl-Histone H3 (Lys27) Antibody

Dot Blot;Multiplexing;Peptide Inhibition Assay;Western Blotting


Multiplexing;Cell Function Assay;Western Blotting;ChIP-seq;Dot BlotWestern Blotting;Peptide Inhibition Assay

ChIP;Western Blotting;Dot Blot

Dot Blot;Immunocytochemistry;Immunofluorescence;Western Blotting;Peptide Inhibition Assay; ChIPWestern Blotting;ChIP-seq; ChIP;Dot Blot;Peptide Inhibition Assay

Western Blotting

Western Blotting;Dot Blot;Multiplexing;ChIP-seq; ChIP

Western Blotting;Peptide Inhibition AssayImmunocytochemistry;Western Blotting

Dot Blot;Immunocytochemistry;Peptide Inhibition Assay;Western BlottingWestern Blotting;Immunocytochemistry

Immunofluorescence;Western Blotting;Peptide Inhibition Assay;Dot Blot

Western Blotting;Immunocytochemistry;Dot BlotImmunofluorescence;Western Blotting;ImmunocytochemistryImmunoprecipitation; Western Blotting;ImmunohistochemistryWestern Blotting;Peptide Inhibition Assay;Multiplexing;Dot BlotWestern Blotting;Peptide Inhibition Assay;Multiplexing

Western Blotting;Immunocytochemistry;Peptide Inhibition Assay;Dot BlotWestern Blotting;Immunocytochemistry;Dot BlotELISA;Immunocytochemistry;Immunoprecipitation;Western Blotting;Multiplexing;Dot BlotWestern Blotting;Immunocytochemistry

Western Blotting;Dot Blot;Multiplexing

Western Blotting;ELISA;MultiplexingImmunocytochemistry;Immunoprecipitation;Western Blotting;Peptide Inhibition Assay;Dot Blot;Multiplexing

Immunocytochemistry;Immunoprecipitation;Multiplexing;Western Blotting;ChIP-seq; Immunohistochemistry

Human;Vertebrates

Human;Tetrahymena;Vertebrates;XenopusHuman

Vertebrates

Mouse;Human;Chicken;Drosophila;Vertebrates;Yeast (S. cerevisiae)Human;Tetrahymena

Human;Chicken

Chicken;Human;Vertebrates

Human;Vertebrates

HumanHuman;Chicken;Vertebrates;Yeast (S. cerevisiae)Human;Mouse;Rat;Chicken;Yeast (S. pombe)Human;Yeast (S. cerevisiae);ChickenHuman

Human;Mouse;Rat

Vertebrates

Bovine;Human;Mouse;Rat

Human

Human

Human;Mouse

Human;Mouse

Human;Vertebrates

Human;Mouse;Rat

Human

Human;Vertebrates

Human

Human;Mouse

200 µL

100 µL

200 µg

100 µL

200 µL

200 µL

100 µL

400 µL

100 µL

100 µL100 µL

100 µg

100 µL

100 µL

100 µg

200 µL

200 µg

100 µL

100 µL

100 µg

100 µL

100 µg

100 µg

100 µg

100 µg

100 µL

200 µg

17

90%的文章使用该抗体进行实验。

参考文献:

PRC2 is recurrently inactivated through EED or

SUZ12 loss in malignant peripheral nerve sheath

tumors.

Lee, W; Teckie, S; Wiesner, T; Ran, L; Prieto Granada,

CN; Lin, M; Zhu, S; Cao, Z; Liang, Y; Sboner, A; Tap,

WD; Fletcher, JA; Huberman, KH; Qin, LX; Viale, A;

Singer, S; Zheng, D; Berger, MF; Chen, Y; Antonescu,

CR; Chi, P Nature genetics 46 1227-32 2014 XACT,

a long noncoding transcript coating the active X

chromosome in human pluripotent cells. Vallot,

Céline, et al. Nat. Genet., 45: 239-41 (2013) 2013


参考文献:

Early-stage epigenetic modi�cation during somatic

cell reprogramming by Parp1 and Tet2.

Claudia A Doege,Keiichi Inoue,Toru Yamashita,

David B Rhee,Skylar Travis,Ryousuke Fujita,Paolo

Guarnieri,Govind Bhagat,William B Vanti,Alan

Shih,Ross L Levine,Sara Nik,Emily I Chen,Asa

Abeliovich Nature 488 2012 The human GFI136N

variant induces epigenetic changes at the Hoxa9

locus and accelerates K-RAS driven myeloproliferative

disorder in mice.

Cyrus Khandanpour, Joseph Krongold, Judith Sch,

Frederique Bouwman, Lothar Vassen, Marie-Claude

Gaudreau, Riyan Chen, Fernando J Calero-Nieto,

Evangelia Diamanti, Rebecca Hannah, Sara E Meyer,

H Leighton Grimes, Bert A van der Reijden, Joop H

Jansen, Chandrashekhar V Patel, Justine K Peeters,

Bob L, Ulrich D, Bertie G, Tarik M

Blood 120 2012


07-549

05-801

07-473

05-745R

04-745

07-442

07-523

05-1242

07-463

04-079

05-1354

Anti-trimethyl-Histone H3 (Lys36) AntibodyAnti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86, rabbit monoclonalAnti-trimethyl-Histone H3 (Lys4) AntibodyAnti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonalAnti-trimethyl-Histone H3 (Lys9) Antibody

Anti-trimethyl-Histone H3 (Lys9) AntibodyAnti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4

Anti-trimethyl-Histone H4 (Lys20) AntibodyAnti-trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonalAnti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5

Dot Blot;Western Blotting

Dot Blot;Western Blotting;Peptide Inhibition Assay

Dot Blot;Western Blotting;ChIP-seq;Chromatin Immunoprecipitation (ChIP)Western Blotting;Chromatin Immunoprecipitation (ChIP);ChIP-seq;Dot Blot;ELISA;Peptide Inhibition Assay;Multiplexing Western Blotting;Dot Blot;Multiplexing;ChIP-seq

Dot Blot; Immunocytochemistry;Multiplexing;Peptide Inhibition Assay;Western Blotting;ChIP-seqDot Blot;Peptide Inhibition Assay;Western BlottingPeptide Inhibition Assay;Immunofluorescence;Dot Blot;ChIP-seq;Western Blotting; ChIPImmunoprecipitation;Western Blotting;Dot Blot; ChIPWestern Blotting;Dot Blot; ChIP


Human

Chicken

Human;Vertebrates

Human

Human

Human;Mouse;Rat;Chicken

Human

Human;Mouse

Human;Mouse;Rat

Human;Vertebrates

Human;Drosophila

100 µL

100 µL

100 µL

100 µL

100 µL

100 µg

200 µL

200 µL

100 µg

100 µL

100 µL


磷酸化及泛素化组蛋白抗体

05-1315

07-081

06-597

05-1324

05-636

16-193

16-202A

07-1590

07-191

16-189

05-806

Anti-phospho (Ser10) acetyl (Lys14) Histone H3 Antibody, clone 10H10.2Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 AntibodyAnti-phospho-Histone H1 AntibodyAnti-phospho-Histone H1 Antibody, clone 12D11Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugateAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugateAnti-phospho Histone H2A.X Antibody (Tyr142)Anti-phospho-Histone H2B (Ser14) AntibodyAnti-phospho-Histone H3 (Ser10) Antibody, biotin conjugateAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10

Western Blotting

Immunocytochemistry;Western Blotting;ChIP-seq; ChIPImmunocytochemistry;Western Blotting

Immunohistochemistry;Immunocytochemistry;Immunoprecipitation;Western BlottingImmunocytochemistry;Immunofluorescence;Western Blotting; ChIPImmunocytochemistry;Western Blotting

Flow Cytometry;Immunocytochemistry

Western Blotting;Dot Blot

ELISA;Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry

Flow Cytometry; Western Blotting;Immunocytochemistry;Immunofluorescence;Peptide Inhibition Assay;Multiplexing

Human

Mouse;Human;Eukaryote

Bovine;Human

Human;Bovine;Mouse;VertebratesVertebrates

Human

Human

Human;Mouse

Human

Human

Human

100 µg

100 µL

200 µg

200 µL

200 µg

100 µg

100 µg

100 µg

200 µL

100 µg

100 µg

18

近70%的文章使用该抗体进行实验

参考文献:

Acetylation limits 53BP1 association with damaged

chromatin to promote homologous recombination.

Tang, Jiangbo, et al.

Nat. Struct. Mol. Biol., 20: 317-25 (2013)

ROS-induced DNA damage and PARP-1 are

required for optimal induction of starvation-

induced autophagy.

José Manuel Rodríguez-Vargas,María José

Ruiz-Magaña,Carmen Ruiz-Ruiz,Jara Majuelos-

Melguizo,Andreína Peralta-Leal,María Isabel

Rodríguez,José Antonio Muñoz-Gámez,Mariano

Ruiz de Almodóvar,Eva Siles,Abelardo López

Rivas,Marja Jäättela,F Javier Oliver Cell research 22

2012


16-218

16-222

05-1336

04-817

05-598

06-570

07-145

04-789

07-424

05-746R

04-746

09-797

07-679

05-1354

17-685

17-10141

AB10029

05-678

05-1312

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 conjugateAnti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC ConjugateAnti-phospho-Histone H3 (Ser10) Antibody, clone CMA312Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonalAnti-phospho-Histone H3 (Ser10) Antibody, clone RR002Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis MarkerAnti-phospho-Histone H3 (Ser28) AntibodyAnti-phospho-Histone H3 (Thr11) Antibody, clone MC83, rabbit monoclonalAnti-phospho-Histone H3 (Thr3) AntibodyAnti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonalAnti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonalAnti-phospho-Histone H3(Ser10) AntibodyAnti-phospho-Histone H3.3 (Ser31) AntibodyAnti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5ChIPAb+ Phospho-Histone H3 (Ser10) - ChIP Validated Antibody and Primer SetChIPAb+™ Phospho-Histone H3 (Thr3) - ChIP Validated Antibody and Primer Set, rabbit monoclonalAnti-Ubiquityl Histone H2A.X (Lys119) AntibodyAnti-ubiquityl-Histone H2A Antibody, clone E6C5Anti-Ubiquityl-Histone H2B Antibody, clone 56

Immunofluorescence;Western Blotting

Western Blotting;Immunocytochemistry;ImmunofluorescenceWestern Blotting;ELISA;Immunocytochemistry;Multiplexing; ChIPImmunofluorescence;Western Blotting;Immunocytochemistry;ChIP-seq;Dot Blot;Multiplexing; ChIPImmunocytochemistry;Immunohistochemistry;Western BlottingImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting;Immunocytochemistry;Dot Blot;Peptide Inhibition Assay; ChIPWestern Blotting;Peptide Inhibition Assay;Multiplexing

Immunofluorescence;Western Blotting;ImmunocytochemistryWestern Blotting;Peptide Inhibition Assay

Western Blotting;Dot Blot;Multiplexing;Peptide Inhibition Assay

Western Blotting;ELISA;ImmunohistochemistryWestern Blotting;Peptide Inhibition Assay


Western Blotting; ChIP


Western Blotting;Immunoprecipitation;ImmunocytochemistryImmunocytochemistry;Western Blotting; ChIPWestern Blotting; ChIP

Human

Human

Human;Vertebrates

Human

Human;Vertebrates;NematodeMouse;Human

Human;Mouse

Human

Human

Human

Human

Human

Human

Human;Drosophila

Green Plants;Vertebrates

Human

Human;Mouse

Human;Rat;Mouse;Monkey;AmphibianHuman;Mouse;Rat

100 µg

100 µg

100 µg

100 µL

200 µg

200 µg

100 µL

200 µL

100 µL

100 µL

100 µL

400 µL

100 µL

100 µL

25 assays

25 assays

100 µL

200 µL

100 µg


经ChIP验证抗体套装（抗体+正对照引物对+负对照抗体）

17-10046

17-675

17-10047

17-615

ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Histone H3 (Unmodified Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set

Western Blotting;Immunoprecipitation;Dot Blot; ChIP




Human;Mouse;Rat;Yeast (S. cerevisiae);Chicken

Vertebrates

Human

Human;Mouse;Tetrahymena

25 assays

25 assays

25 assays

25 assays

19

65%的文章使用该抗体进行实验

参考文献:

Human oocytes reprogram adult somatic nuclei of

a type 1 diabetic to diploid pluripotent stem cells.

Yamada, Mitsutoshi, et al.

Nature, 2014 DARPP-32 interaction with adducin

may mediate rapid environmental effects on

striatal neurons. Engmann O, Giralt A, Gervasi N,

Marion-Poll L, Gasmi L, Filhol O, Picciotto MR,

Gilligan D, Greengard P, Nairn AC, Hervé D, Girault

JA. Nat Commun. 2015 Dec 7


17-10051

17-683

17-10050

17-658

17-609

17-630

17-10101

17-10045

17-10112

17-10111

17-10241

17-643

17-676

17-680

17-651

17-10108

17-677

17-648

17-681

17-10125

17-10130

ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Acetyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set ChIPAb+Acetyl- Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H3 (Lys9) Purified - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H3 (Lys9) Serum - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H4 (Lys5) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+™ Acetyl Histone H3 (Lys23) - ChIP Validated Antibody and Primer SetChIPAb+™ Acetyl-Histone H3 (Lys18) - ChIP Validated Antibody and Primer SetChIPAb+™ Acetyl-Histone H3 (Lys9/18) - ChIP Validated Antibody and Primer SetChIPAb+ Monomethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set

ChIPAb+ Monomethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Monomethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+ Monomethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer SetChIPAb+ Dimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Dimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+™ Dimethyl-Histone H3 (Lys79) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+™ Trimethyl-Histone H3 (Lys79) - ChIP Validated Antibody and Primer Set

ChIP;ChIP-seq;Western Blotting;Immunocytochemistry;Immunoprecipitation;Immunohistochemistry (Paraffin)Western Blotting; ChIP


Western Blotting;Immunocytochemistry; ChIP;ChIP-seq


Western Blotting;Multiplexing;Cell Function Assay; ChIP

Immunoprecipitation; Western Blotting; ChIP

Western Blotting;Immunoprecipitation; ChIP

Immunoprecipitation; Western Blotting; ChIP

Western Blotting;Dot Blot; ChIP; ChIP-seq


Flow Cytometry;Electrophoretic Mobility Shift Assay;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting; ChIPWestern Blotting; ChIP

Western Blotting;ChIP-seq; ChIP


ChIP;Western Blotting;Cell Function Assay

Western Blotting; ChIP; ChIP-seq;Dot Blot;Multiplexing

Western Blotting;Immunoprecipitation; ChIP



Western Blotting; ChIP; Multiplexing

Human;Rat

Vertebrates

Human

Human;Mouse;Mammals

Human;Mouse;Mammals

Human;Eukaryote

Human;Vertebrates

Human

Human;Vertebrates;Yeast (S. cerevisiae)

Human;Vertebrates;Yeast (S. cerevisiae)

Bovine;Human;Mouse;Yeast (S. cerevisiae)

Human;Mouse;Mammals

Vertebrates

Vertebrates

Human;Green Plants;Vertebrates

Vertebrates

Vertebrates

Human;Mouse;Mammals

Vertebrates

Human;Vertebrates

Human;Vertebrates

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

25 assays

AB9730

AB9746

AB9732

MABC749

ABE1007AB10410

AB1579407-53507-822

05-797R

05-1152

07-507

MABE329

MABS1190

06-225

MABS15006-47806-659

06-828

05-107607-1333

05-1072

05-1028

MABS64

ABS29

09-272

ABS29

05-747

07-64107-852

07-641

Anti-14-3-3 β Antibody

Anti-14-3-3 ζ Antibody

Anti-14-3-3 ε Antibody, CT

Anti-phospho-BAD (Ser112), clone EPR1891(2), Rabbit Monoclonal AntibodyAnti-BATF3 AntibodyAnti-YES (Proto-oncogene c-Yes) AntibodyAnti-Dexras1 AntibodyAnti-MKP1 AntibodyAnti-phospho-eIF2Bε (Ser539) AntibodyAnti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonalAnti-Erk1/2 (p44/p42) Antibody, clone MK12

Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) AntibodyAnti-c-Fos Antibody, clone 2G9C3Anti-Glucokinase/GCK Antibody, clone 9G1.2 Anti-c-Jun Antibody

Anti-HGK Antibody, clone 2H1.1Anti-HSP27 AntibodyAnti-phospho-c-Jun (Ser73) AntibodyAnti-phospho-c-Jun (Ser63) AntibodyAnti-c-Jun Antibody, clone 6E4.4Anti-JunB Antibody

Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2Anti-p38/SAPK2A Antibody

Anti-phospho-p38α (Thr180/Tyr182) AntibodyAnti-p38/SAPK2A Antibody

Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonalAnti-MEK1 AntibodyAnti-phospho-MEK1 (Thr292) AntibodyAnti-MEK1 Antibody

ELISA;Western Blotting;Immunohistochemistry



Western Blotting

Western Blotting;Flow CytometryWestern Blotting

Western BlottingWestern BlottingWestern Blotting

Multiplexing;Western Blotting

Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);ImmunocytochemistryWestern Blotting;Peptide Inhibition Assay


Western Blotting;Immunohistochemistry (Paraffin)Immunoprecipitation;Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting

Western Blotting;ImmunocytochemistryImmunocytochemistry;Western Blotting

Western Blotting;Immunohistochemistry (Paraffin)Immunofluorescence;ELISA;Western Blotting


Western Blotting

Immunoprecipitation; Western Blotting

Western Blotting

Western Blotting

Immunoprecipitation; Western BlottingWestern Blotting


Bovine;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepHuman

HumanHuman;Horse

MouseHuman;MouseHuman;Rat

Human;Mouse;Rat

Human;Mouse;Rat;Canine;Xenopus;Bovine;Chicken

Human;Mouse;Rat;Bovine

Human

Human

Avian;Human;Mouse

RatHumanHuman;Mouse

Human;Mouse;Rat

Human;Mouse;RatHuman;Mouse;Rat;Canine;Horse;BovineHuman;Mouse;Rat

Human;Mouse;Rat

Human;Mouse

Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat


Human;Mouse;RatHuman;Mouse

Human;Mouse;Rat

100 µL

100 µL

100 µL

100 µL

100 µg100 µg

100 µL100 µg100 µL

100 µL

100 µg

200 µL

100 µg

100 µg

200 µL

100 µg250 µg100 µL

100 µL

100 µg100 µg

100 µg

100 µg

100 µg

100 µg

100 µL

100 µg

100 µL

200 µg100 µL

200 µg

20


17-622

17-10032

17-614

17-678

17-625

17-671

17-685

17-10131

17-10141

17-650

ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer SetChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+ Trimethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Phospho-Histone H3 (Ser10) - ChIP Validated Antibody and Primer SetChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer SetChIPAb+™ Phospho-Histone H3 (Thr3) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set

Western Blotting;ChIP

ChIP;Western Blotting;Cell Function Assay;Dot Blot

Western Blotting; ChIP;ChIP-seq

Western Blotting; ChIP;ChIP-seq




Electrophoretic Mobility Shift Assay;Immunohistochemistry;Immunoprecipitation;Western Blotting; ChIPWestern Blotting;Dot Blot; ChIP

Western Blotting;Immunofluorescence; ChIP

Human;Mouse

Human;Chicken

Human;Mouse;Mammals

Human;Vertebrates

Vertebrates

Human;Green Plants;Vertebrates

Green Plants;Vertebrates

Human;Rat;Mouse;Hamster

Human

Mouse;Human;Vertebrates;Rat;Green Plants

25 assays

25 assays

25 assays

25 assays

25 assays

10 assays

25 assays

25 assays

25 assays

25 assays

07-853

07-69136-010

ABS94

ABC3607-865

07-250

05-577

05-421

07-604

05-389

07-45307-814

05-516

07-147706-918

06-74807-175

05-986MABS771

06-11807-33705-184

04-889

07-791

05-677

07-909

MAB4369

06-133606-1425

Anti-phospho-MEK1 (Thr386) AntibodyAnti-MEKK3 AntibodyAnti-phospho-MKK7/SKK4 (Thr275/Ser277) AntibodyAnti-MLK1 Antibody

Anti-PEA-15 AntibodyAnti-phospho-PED/PEA-15 (Ser116) AntibodyAnti-PP2A Antibody, A subunit

Anti-PP2A Antibody, C subunit, demethylated, clone 4b7Anti-PP2A Antibody, C subunit, clone 1D6

Anti-Rac2 Antibody

Anti-Rac1 Antibody, clone 23A8

Anti-B-Raf AntibodyAnti-phospho-c-Raf (Ser338/Tyr340) AntibodyAnti-Ras Antibody, clone RAS10

Anti-Rsk1 AntibodyAnti-Rsk2/MAPKAP Kinase 1b AntibodyAnti-JNK/SAPK1 AntibodyAnti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) AntibodyAnti-JNK2 AntibodyAnti-JNK3, clone EPR5493(2), Rabbit Monoclonal AntibodyAnti-SHP-2/SHPTP-2 AntibodyAnti-SOS1 Antibody, CTAnti-Src Antibody, clone GD11

Anti-Src Antibody, clone N6L, rabbit monoclonalAnti-phospho-Src Antibody (Tyr215)Anti-phospho-Src (Tyr416) Antibody, clone 9A6Anti-phospho-Src (Tyr418) AntibodyAnti-SRF [Serum Response Factor] Antibody, clone 1E1Anti-TAB1 AntibodyAnti-phospho-TAK1 (Ser412) Antibody

Western Blotting

Western BlottingWestern Blotting

Immunohistochemistry (Paraffin)

Western Blotting;ImmunohistochemistryWestern Blotting


Immunocytochemistry;Immunoprecipitation;Western BlottingImmunocytochemistry;Immunoprecipitation;Western Blotting

Immunocytochemistry;Immunoprecipitation;Western BlottingImmunohistochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;Western BlottingWestern Blotting

ELISA;Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation; Western BlottingWestern Blotting;Immunoprecipitation;ELISAImmunoprecipitation;Western Blotting

Immunoprecipitation; Western BlottingWestern Blotting;Enzyme Assay;Immunoprecipitation

ELISA;Western BlottingWestern Blotting;Immunocytochemistry;Flow CytometryWestern Blotting;ImmunocytochemistryImmunoprecipitation; Western BlottingImmunoprecipitation; Western Blotting

Western Blotting


Western Blotting;Immunoprecipitation

Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry


Human;Mouse;Rat

Human;Mouse;RatHuman

Human;Rat;Mouse;Chimpanzee;Rhesus MacaqueHuman;Mouse;RatHuman;Mouse

Human;Mouse;Pig;Rat;XenopusVertebrates

Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast S. cerevisiae

Human;Mouse

Human;Mouse;Rat


Human;Rat;Mouse

Human;Mouse;RatHuman;Mouse;Rat

Human;Mouse;RatMouse;Human;Rat;Canine;Xenopus;Chicken;Bovine;HorseHuman;Mouse;RatHuman

Human;MouseHuman;Mouse Avian;Human;Mouse;Mink;Rat

Human

Human

Human;Mouse

Chicken;Human;Mouse

Human;Mouse

Human;Mouse;Rhesus MacaqueHuman;Mouse

100 µL

100 µL100 µL

100 µg

100 µg100 µL

100 µg

200 µL

200 µg

50 µL

250 µg

200 µg100 µL

100 µg

100 µg100 µg

200 µg200 µg

100 µg100 µL

200 µg200 µg200 µg

100 µl

100 µL

100 µg

100 µL

100 µg

100 µg200 µL

21

Vainchenker, W., and Constantinescu, S.N., 2013. Oncogene 32, 2601.Yu, H., et al. 2009. Nat. Rev. Cancer 9, 798.Pfeifer, A.C., et al. 2008. Essays Biochem. 45, 109.Rawlings, J.S. et al. 2004. J. Cell Sci. 117, 1281.Shuai, K., and Liu, B. 2003. Nat. Rev. Immunol. 3, 900.

参考文献

JAK/STAT SignalingJAK/STAT型号通路在细胞生长、分化、迁移及死亡等的基本细胞功能中发挥着中要作用。白细胞中表达

了许多JAK-STAT通路，并因此参与免疫系统的调控。

来自干扰素、白细胞介素、生长因子或其它化学信使的信号可以激活该信号通路受体，从而激活了JAK的激酶功能，导致对其自身的磷酸化（磷酸基团作为蛋白质上的开关）；接下来STAT蛋白结合到被磷酸

化的受体上，在此STAT被JAK磷酸化；被磷酸化的STAT蛋白结合到另一个被磷酸化的STAT蛋白上（二聚

化）并易位到细胞核中；在细胞核中，它结合到DNA上并启动转录那些响应STAT的基因。

JAK-STAT途径是进化保守的，从黏菌和蠕虫到哺乳动物（但真菌或植物则不是）。若JAK-STAT的功能被打

乱或失调会导致免疫缺陷综合征及癌症。

Nucleus

Growth Factors

RTK

Growth Hormones

Growth Hormone Receptors

JAKsJAKs

Cytokines GPCR Ligands

Cytokine Receptor GPCR

• AG 490 • JAK2 Inhibitor II • JAK Inhibitor I

• PP2, Indirubin Derivative

• STAT5 Inhibitor

• STAT5 Inhibitor

• STAT3 Inhibitor V, Stattic • STAT3 Inhibitor Peptide, Cell Permeable • STAT3 Inhibitor III, WP1066

• STAT3 Inhibitor V, Stattic • P3-AHNP Peptide Carrier• STAT3 Inhibitor III, WP1066

• STAT3 Inhibitor V, Stattic • P3-AHNP Peptide Carrier• STAT3 Inhibitor III, WP1066

• SHP1/2 PTPase Inhibitor, NSC-87877

• AG 490• JAK2 Inhibitor II,

• JAK Inhibitor I

• AG 490 • JAK2 Inhibitor II • JAK Inhibitor I

• c-Myc Inhibitor

• PD 153035

• LY 294002

• U0126

Akt Pathway

Gene Expression

STAT3STAT5

JAK2

Src

JAKs

Akt

Raf

MEK

ERKs

SHP2JAKs JAKs

Cofactorsc-Myc

CTFs

STATsSTATs

STAT3STAT3

STATs

STATs STATs

STATs

STATsSTATs

STATsSTATs

STATsSTATs

STAT5STAT5

STATsSTATs

JAK2

Sumo SumoSumo

RANKPNA1

STAM

SH2B

PI 3-K

GRB2SOS Ras

Phosphorylation

Calbiochem® Inhibitors

Description Species Reactivity* Applications** Qty/Pk Catalogue No.

Anti-JAK1 H, Ht, M, R, Rb IP, WB 150 µL 06-272

Anti-JAK1 H, M, R IP, WB 100 µL AB3802

Anti-JAK1 H, M, R WB 100 µg 07-1485

Anti-JAK1, clone 73 H, M, R, Ca, Xn, Ch IF, IP, WB 100 µg 05-1154

Anti-JAK2 H, M, R IP, WB 150 µL 06-255

Anti-JAK2 H, M, R IP, WB 100 µg 06-1310

Anti-JAK2, Agarose conjugate H, M, R IP 200 µg 16-121

Anti-JAK2, clone 8E10.2 H, M, R IH(P), WB 100 µL 04-001

Anti-phospho-JAK2 (Tyr317) H WB 100 µL 07-1318

JAK/STAT 信号通路研究抗体

参考文献:

Virally infected and matured human dendritic cells

activate natural killer cells via cooperative activity

of plasma membrane-bound TNF and IL-15.

Blood 116 575-83. Epub 2010 Apr 29. 2010

The mitogen-activated protein kinase and JAK-STAT

signaling pathways are required for an oncostatin

M-responsive element-mediated activation of

matrix metalloproteinase 1 gene expression. J. Biol.

Chem., 272: 1188-96 (1997) 1997

22

Anti-phospho JAK2 (Tyr570) H, R, Eq, Chp, Pm, Po

WB 100 µg 09-241

Anti-phospho JAK2 (Tyr813) H, M, R, Eq, Chp, Po WB 100 µg 09-242

Anti-phospho JAK2 (Tyr972) H, R, Eq, Op, B, Chp, Po

WB 100 µg 09-243

Anti-phospho-JAK2 (Tyr1007/1008) H, Ch, M, Po, R, Xn, Zebra�sh

WB 200 µL 09-275

Anti-phospho-JAK2 (Tyr1007/1008) H, M, R WB 200 µg 07-606

Anti-phospho-JAK2 (Tyr1007/Tyr1008), clone E132 H, M, R ICC, IH(P), IP, WB 100 µL 04-1098

Anti-Phospho-JAK1 (TYR1022/1023) H, M, R WB 100 µL 07-1489

Anti-JAK3 M WB 150 µL 06-342

Anti-JAK3 H, M IP, WB 100 µL 07-1487

Anti-JAK3, clone B32-32 H, M IP 200 µg 05-406

Anti-JAK3, clone HL423 H, M Mplex, WB 200 µL 04-011

Anti-JAK3, CT H WB 250 µg 06-667

Anti-STAT1 H WB 100 µL 04-069

Anti-STAT1, CT H, M EMSA, IP, WB 250 µg 06-501

Anti-STAT1-α H, M IP, WB 100 µg AB16951

Anti-Phospho-STAT1 (Tyr701), clone 5C9.2 H, M, R WB 100 µg 05-1064

参考文献:

JAK2, but not Src family kinases, is required for

STAT, ERK, and Akt signaling in response to growth

hormone in preadipocytes and hepatoma cells.

Molecular endocrinology (Baltimore, Md.) 22

1825-41, 2008

alpha-Syntrophin regulates ARMS localization at

the neuromuscular junction and enhances EphA4

signaling in an ARMS-dependent manner. J Cell

Biol, 169:813-24, 2005

参考文献:

c-Abl Activates Janus Kinase 2 in Normal

Hematopoietic Cells.doi: 10.1074/jbc.M114.554501

A Novel Role for Histone Deacetylase 6 in the

Regulation of the Tolerogenic STAT3/IL-10 Pathway

in APCs.doi: 10.4049/ jimmunol.1302778

JAK/STAT 信号通路研究抗体


Anti-phospho-STAT1 (Tyr701) Ch, H, M, R WB 100 µL 07-307

Anti-phospho-STAT1 (Tyr701), Alexa Fluor® 488 conjugated, clone 5C9.2

H, M, R FC, WB 100 tests FCMAB106A4

Anti-phospho-STAT1 (Ser727) H, M WB 200 µL 07-714

Anti-phospho-STAT1 (Ser 727), clone 12C5 H ELISA, WB 100 µg 04-478

Anti-STAT2 M ICC, EMSA, IP, WB 200 µg 07-140

Anti-STAT2, CT H, M ICC, EMSA, IP, WB 200 µg 06-502

Anti-phospho-STAT2 (Tyr689) H, M IP, WB 100 µL ABE541

Anti-phospho-STAT2 (Tyr689) H, M IP, WB 200 µL 07-224

Anti-STAT3 H, M, R ICC, EMSA, IP, WB 200 µg 06-596

Anti-STAT3, clone 18F7.1 H, M ICC, WB 100 µg 05-1078

Anti-STAT3, clone E121-21 H ICC, IH(P), IP, WB 100 µL 04-1014

Anti-phospho-STAT3 (Tyr68) Ch, B, H, M, Po, R, Xn

WB 100 µg 07-1347

Anti-phospho-STAT3 (Tyr705), clone 9E12 Ca, H, M ELISA, IP, WB 100 µg 05-485

Anti-phospho-STAT3 (Ser727) M WB 100 µL 07-703

Anti-STAT5A H, M, R IP, WB 100 µL 06-968

Anti-phospho-STAT5A/B (Ser726/Ser731) H, M, R WB 200 µL 06-867

Anti-STAT5A/B H, M, R, Sh EMSA 250 µL 06-588

Anti-STAT5B H, M WB 200 µg 06-554

Anti-STAT5B Av, H, M, R IP, WB 100 µL 06-969

Anti-STAT5α (CT), clone E289 H, R ICC, FC, IH(P), IP, WB 100 µL 04-1016

Anti-phospho-STAT5A/B (Tyr694/699), clone 8-5-2

B, H, M, R, Sh WB 100 µg 05-495

Anti-phospho-STAT5A/B (Tyr694/699) B, H, M, R DB, WB 100 µL 07-586

Anti-phospho-STAT5A/B (Tyr694/699), clone A11W

B, Chp, H, M, R WB (Not IP) 100 µL 04-886

Anti-phospho-STAT5A/B (Tyr694/699), clone A11W H, M WB 100 µL 05-886

参考文献:

High STAT1 mRNA levels but not its tyrosine

phosphorylation are associated with macrophage

in�ltration and bad prognosis in breast cancer.

BMC Cancer 2014, 14:257

STAT2 de�ciency and susceptibility to viral illness in

humans.PNAS, February 19, 2013


23

*Species: Av: avian; B: bovine, Ca: canine; Ch: chicken; Chp: chimpanzee; Eq: equine; H: human; M: mouse, Op: opossum; Pm: primate;R: rat; Sh: sheep; Xn: xenopus**Applications: ELISA: enzyme-linked immunosorbent assay; EMSA: electrophoretic mobility shift assay; FC: �ow cytometry; ICC: immunocytochemistry,IF: immuno�uorescence; IH: immunohistochemistry; IP: immunoprecipitation; Mplex: multiplexing; WB: Western blotting

JAK 抑制剂Description Catalogue No. Comments Size

JAK Inhibitor I (Pyridone 6) 420099 A cell-permeable, reversible, ATP-competitive inhibitor of JAK1 (IC50 = 15 nM for murine JAK1), JAK2 (IC50 = 1 nM), JAK3 (Ki = 5 nM), and Tyk2 (IC50 = 1 nM).

500 µg 1 mg

InSolution™ JAK Inhibitor I 420097 A 10 mM (500 µg/162 µL) solution of JAK Inhibitor I (Cat. No. 420099) in DMSO.

500 µg

JAK2 Inhibitor II 1,2,3,4,5,6-Hexabromo-cyclohexane

420132 A cell-permeable, speci�c, reversible, and direct inhibitor of JAK2 autophosphorylation (maximal inhibition at 50 µM in BSC-40 cells overexpressing JAK2).

25 mg

JAK2 Inhibitor III, SD-1029 NSC 371488

573098 A cell-permeable, selective inhibitor of JAK2. 10 mg

JAK2 Inhibitor IV 3-Amino-5-(N-tert-butyl-sulfonamido-4-phenyl)-indazole

420139 A potent inhibitor of both the wild-type JAK2 and the constitutively active V617F mutant (IC50 = 78 and 206 nM, respectively).

5 mg

JAK2 Inhibitor IX, WP1193 420151 A cell-permeable analog of WP1130 (Cat. No. 681685) that inhibits JAK2 kinase activity and suppresses JAK2-dependent STAT3 Tyr705 phosphorylation.

10 mg

JAK2 Inhibitor V, Z3 2-Methyl-1-phenyl-4-pyridin-2-yl-2-(2-pyridin-2-ylethyl)butan-1-one

420141 A cell-permeable inhibitor of autophosphorylation of both the wild-type JAK2 and the constitutively active V167F mutant (IC50 = 15 and 28 µM, respectively).

10 mg

JAK3 Inhibitor I WHI-P131 420101 A cell-permeable, reversible, potent, ATP-competitive, and speci�c inhibitor of JAK3 (IC50 = 78 µM).

5 mg

JAK3 Inhibitor II WHI-P154

420104 A potent, cell-permeable, reversible, ATP-competitive, and speci�c inhibitor of JAK3 with no effect on JAK1 and JAK2.

5 mg

JAK3 Inhibitor, Negative Control; WHI-P258, HCl

420112 A useful negative control for JAK3 Inhibitors (IC50 > 300 µM).

1 mg

JAK3 Inhibitor IV ZM 39923

420121 A potent, selective, and ATP-competitive inhibitor of Janus tyrosine kinase-3 (JAK3) (pIC50 = 7.1).

10 mg

JAK3 Inhibitor V ZM 449829

420122 A breakdown product of JAK3 Inhibitor IV (Cat. No. 420121) with similar inhibitory potency (pIC50 = 6.8 for JAK3)

10 mg

JAK3 Inhibitor VI 420126 A cell-permeable, potent, and reversible inhibitor of JAK3 (IC50 = 27 nM).

5 mg

JAK3 Inhibitor VII, AD412 N-(Pyridin-4-yl)-3-[1-(4-chloro- benzyl)indol-3-yl]-propanamide

420145 A cell-permeable, selective inhibitor of JAK3 (by 81% and 36% at 90 and 30 µM, respectively)

25 mg

JAK3 Inhibitor VIII NSC114792 420146 A cell-permeable compound that targets JAK3 kinase domain and selectively inhibits JAK3, but not JAK1, JAK2, or TYK2.

10 mg

Anti-phospho STAT6 (Tyr641), clone 19E2.1 H, M FC, WB 100 µg MABE270

Anti-phospho-STAT6 (Tyr641), clone 16E12 H, M, R WB 100 µg 05-590

Anti-phospho-STAT6 (Tyr641) H, M WB 100 µL 06-937


24

STAT 抑制剂Description Catalogue No. Comments Size

STAT3 Inhibitor Peptide Ac-PpYLKTK-OH

573095 A STAT3-SH2 domain binding phosphopeptide that acts as a selective inhibitor of STAT3 signaling (DB50 = 235 µM).

1 mg

STAT3 Inhibitor Peptide, Cell-Permeable PpYLKTK-mts

573096 A cell-permeable analog of the STAT3-SH2 domain-binding phosphopeptide (Cat. No. 573095) that contains a C-terminal membrane translocating sequence (MTS).

1 mg 5 mg

STAT3 Inhibitor III, WP1066 573097 A cell-permeable analog of AG 490 (Cat. No. 658401) that acts as a STAT3 pathway inhibitor. Inhibits malignant glioma growth (IC50 = 5.6 and 3.7 µM in U87-MG and U373-MG cells, respectively).

10 mg

InSolution™ STAT3 Inhibitor III, WP1066

573129 A 50 mM (5 mg/281 µL) solution of STAT3 Inhibitor III, WP1066 (Cat. No. 573097) in DMSO.

5 mg

STAT3 Inhibitor V, Stattic 6-Nitrobenzo[b]thiophene-1, 1-dioxide

573099 A cell-permeable inhibitor of STAT3 cellular function that targets the STAT3-SH2 domain and prevents its association with upstream kinases.

25 mg

STAT3 Inhibitor VI, S3I-201 NSC 74859

573102 A cell-permeable compound that binds STAT3-SH2 domain and prevents STAT3 phosphorylation/activation, dimerization, DNA-binding, and STAT3-dependent transcription.

10 mg 25 mg

InSolution™ STAT3 Inhibitor VI, S3I-201

573130 A 50 mM (5 mg/274 µL) solution of STAT3 Inhibitor VI, S3I-201 (Cat. No. 573102) in DMSO.

5 mg

STAT3 Inhibitor VII Ethyl-1-(4-cyano-2,3,5,6-tetra-�uorophenyl)-6,7,8-tri�uoro-4-oxo-1,4-dihydroquinoline-3-carboxylate

573103 A cell-permeable, potent inhibitor of JAK1/JAK2/TYK2/STAT3 activation (IC50 = 170 nM against U266 constitutive STAT3 Y705 phosphorylation).

5 mg

STAT3 Inhibitor VIII, 5,15-DPP 5,15-Diphenylporphyrin

573109 A cell-permeable compound that directly binds to STAT3 and prevents its dimerization. Acts as a STAT3-SH2 domain antagonist (IC50 = 280 nM).

25 mg

STAT3 Inhibitor X, HJB 17-Hydroxy-jolkinolide B

573106 A highly selective inhibitor of JAK/STAT3 signaling with anti-tumor properties (~12 µM). Suppresses IL-6-induced STAT-3 transcription activity (IC50= ˜ 3.34 µM in HepG2 cells).

1 mg

STAT3 Inhibitor Peptide, Cell-permeable, Inactive Control PYLKTK-mts

573105 A cell-permeable, non-phosphorylated inactive control peptide for STAT3 cellular function studies.

5 mg

STAT3 Inhibitor IX, Cpd188 4-((3-(Carboxymethylsulfanyl)-4-hydroxy-1-naphthyl)sulfamoyl)-benzoic acid

573125 A cell-permeable compound that inhibits IL-6-stimulated STAT3 Tyr705 phosphorylation (IC50 = 73 µM) and nuclear translocation (IC50 = 39 µM) in HepG2 cells.

10 mg

STAT3 Inhibitor XI, STX-0119 N-(5-(Furan-2-yl)-1,3,4-oxadiazol-2-yl)-2-phenylquinoline-4-carboxamide

573126 A cell-permeable, selective inhibitor of STAT3 DNA binding activity. Prevents IL-6-stimulated STAT3 dimerization in HEK293 cells (IC50 = 74 µM).

25 mg

STAT3 Inhibitor XII, SPI H2N-FISKERERAILSTKPP-GTFLLRFSESSK-CO2H

573127 A cell-permeable STAT3-SH2 domain-derived (588-615) 28-mer peptide that competitively prevents STAT3 binding to cognate pTyr peptide motif, represses STAT3 dimerization and induces apoptosis.

5 mg

STAT3 Inhibitor XIII, C188-9 F1113-0789

573128 A cell-permeable binaphthol-sulfonamide that competes against pY proteins for STAT3-SH2 domain binding.

10 mg

STAT3 Inhibitor XIV, LLL12 5-Hydroxy-9,10-dioxo-9,10-dihydroanthracene-1-sulfonamide

573131 A cell-permeable compound that binds directly to the phosphoryl Tyr705 binding site of the STAT3 monomer and acts as selective and reversible inhibitor of STAT3 signaling.

10 mg

STAT3 Inhibitor XVIII, BP-1-102 573132 A cell-permeable compound that selectively binds the three subpockets of STAT3- SH2 domain surface (KD = 504 nM) and blocks STAT3 phosphorylation, dimerization, and DNA-binding.

10 mg

STAT5 Inhibitor II, IQDMA N1-(11H-indolo[3,2-c]quinolin-6-yl)-N2,N2-dimethylethane-1,2-diamine

420294 A cell-permeable inhibitor of STAT5 signaling in HL-60 and K562 cells and inhibits the growth of various tumor cells (IC50 = 8, 5, and 8 µM for A549, HL-60, and K562 cells, respectively).

10 mg

25

STAT5 Inhibitor N‘-((4-Oxo-4H-chromen-3-yl)methylene)nicotinohydrazide

573108 A cell-permeable compound that selectively targets the SH2 domain of STAT5 (IC50 = 47 µM against STAT5b SH2 domain EPO peptide binding activity).

10 mg

STAT5 Inhibitor III, Pimozide 573110 A cell-permeable compound that inhibits the constitutive STAT5 Tyr694 phosphorylation and transcription activity in Bcr-Abl+ K562 and KU812 cultures.

100 mg

STAT3/STAT5 DualInhibitor, SH-4-54 509105 A cell-permeable, salicyclic-to-benzoic acid-substituted BP-1-102 (Cat. No. 573132) analog that targets STAT3 and STAT5 with comparable af�nity (koff/kon = KD = 300 and 464 nM, respectively).

10 mg

Description Qty/Pk Catalogue No.

PD 153035 1 mg 234490

Indirubin Derivative E804 1 mg 402081

JAK Inhibitor I 500 µg 420099

JAK2 Inhibitor II 25 mg 420132

LY 294002 5 mg 440202

PP2 1 mg 529573

SHP1/2 PTPase Inhibitor, NSC-87877 50 mg 565851

STAT3 Inhibitor Peptide, Cell Permeable 1 mg 573096

STAT3 Inhibitor III, WP1066 10 mg 573097

STAT3 Inhibitor V, Stattic 25 mg 573099

STAT5 Inhibitor 10 mg 573108

AG490 5 mg 658401

U0126 1 mg 662005

DMSO 15 mL KP31817

JAK/STAT 信号通路抑制剂套装(Catalogue No. 420138)

The panel contains the following inhibitors and one vial of high quality DMSO.

Description Applications Qty/Pk Catalogue No.

JAK1, RNA probe, Hu, Cy3 RNA Expression 250 rxns SF-1288






Description Applications Qty/Pk Catalogue No.

STAT1 Activation Dual Detection Kit Flow Cytometry 25 tests FCCS025142

Phospho STAT1(Tyr701) Milliplex Assay Multiplexed ELISA 96 well 46-655MAG

Total STAT1 Milliplex Assay Multiplexed ELISA 96 well 46-654MAG

JAK/STAT RNA 检测

JAK/STAT 试剂盒

Description Catalogue No. Comments Size

26

17-10046

17-675

17-10047

17-615

ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Histone H3 (Unmodified Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set

Western Blotting;Immunoprecipitation;Dot Blot; ChIP




Human;Mouse;Rat;Yeast (S. cerevisiae);Chicken

Vertebrates

Human

Human;Mouse;Tetrahymena

25 assays

25 assays

25 assays

25 assays

MAPK Signaling丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）是一类进化保守性很高的蛋白丝/苏氨酸激酶。因细胞受到细胞因

子、神经递质、激素、细胞应激及细胞黏附等胞外刺激时产生。结合其他几种信号通路一起调节一些转录因子的磷酸化状态。

该信号通路调节着细胞的生长、分化、对环境的应激适应、炎症反应等多种重要的细胞生理/病理过程。MAPK活性异常会导致

肿瘤的发生。


抗体

AB9730

AB9746

AB9732

MABC749

ABE1007AB10410

AB1579407-53507-822

05-797R

05-1152

07-507

MABE329

MABS1190

06-225

MABS15006-47806-659

06-828

05-107607-1333

05-1072

05-1028

MABS64

ABS29

09-272

ABS29

05-747

07-64107-852

07-641













Western Blotting










Western Blotting


Western Blotting

Western Blotting




HumanHuman;Horse


Human;Mouse;Rat



Human

Human

Avian;Human;Mouse

RatHumanHuman;Mouse

Human;Mouse;Rat


Human;Mouse;Rat

Human;Mouse




Human;Mouse;Rat

100 µL

100 µL

100 µL

100 µL

100 µg100 µg

100 µL100 µg100 µL

100 µL

100 µg

200 µL

100 µg

100 µg

200 µL

100 µg250 µg100 µL

100 µL

100 µg100 µg

100 µg

100 µg

100 µg

100 µg

100 µL

100 µg

100 µL

200 µg100 µL

200 µg

27


抗体

AB9730

AB9746

AB9732

MABC749

ABE1007AB10410

AB1579407-53507-822

05-797R

05-1152

07-507

MABE329

MABS1190

06-225

MABS15006-47806-659

06-828

05-107607-1333

05-1072

05-1028

MABS64

ABS29

09-272

ABS29

05-747

07-64107-852

07-641













Western Blotting










Western Blotting


Western Blotting

Western Blotting




HumanHuman;Horse


Human;Mouse;Rat



Human

Human

Avian;Human;Mouse

RatHumanHuman;Mouse

Human;Mouse;Rat


Human;Mouse;Rat

Human;Mouse




Human;Mouse;Rat

100 µL

100 µL

100 µL

100 µL

100 µg100 µg

100 µL100 µg100 µL

100 µL

100 µg

200 µL

100 µg

100 µg

200 µL

100 µg250 µg100 µL

100 µL

100 µg100 µg

100 µg

100 µg

100 µg

100 µg

100 µL

100 µg

100 µL

200 µg100 µL

200 µg

07-853

07-69136-010

ABS94

ABC3607-865

07-250

05-577

05-421

07-604

05-389

07-45307-814

05-516

07-147706-918

06-74807-175

05-986MABS771

06-11807-33705-184

04-889

07-791

05-677

07-909

MAB4369

06-133606-1425




Anti-Rac2 Antibody





Western Blotting










Western Blotting





Human;Mouse;Rat




Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast (<italic>S. cerevisiae</italic>)Human;Mouse

Human;Mouse;Rat


Human;Rat;Mouse



Human;MouseHuman;MouseAvian;Human;Mouse;Mink;RatHuman

Human

Human;Mouse

Chicken;Human;Mouse

Human;Mouse


100 µL

100 µL100 µL

100 µg

100 µg100 µL

100 µg

200 µL

200 µg

50 µL

250 µg

200 µg100 µL

100 µg

100 µg100 µg

200 µg200 µg

100 µg100 µL

200 µg200 µg200 µg

100 µl

100 µL

100 µg

100 µL

100 µg

100 µg200 µL

28


抗体

InhibitorSelect™MAK Kinase Signaling Pathway Inhibitor Panel,目录号444189

组分靶标包装量目录号

ERK InhibitorⅡ,FR180204

JNK InhibitorⅡJNK Inhibitor IXMEK1/2 Inhibitor MNK1 InhibitorMK2a Inhibitorp38 MAP Kinase Inhibitoy VPD 98059Raf Kinase Inhibitor ⅣSB 203580 p38TpI2 Kinase InhibitorZM 336372DMSO

ERK1,ERK2JNK JNK2,JNK3MEK1/2MNK1MK2ap38,CK1MEKB-RafMAPKTpI2 Kinasec-Raf-

1 mg5 mg5 mg1 mg5 mg5 mg1 mg5 mg1 mg1 mg1 mg1 mg15 mL

328007420119420136444939454861475863506156513000553014559389616373692000KP31817


抑制剂

07-853

07-69136-010

ABS94

ABC3607-865

07-250

05-577

05-421

07-604

05-389

07-45307-814

05-516

07-147706-918

06-74807-175

05-986MABS771

06-11807-33705-184

04-889

07-791

05-677

07-909

MAB4369

06-133606-1425




Anti-Rac2 Antibody





Western Blotting










Western Blotting





Human;Mouse;Rat




Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast S. cerevisiae

Human;Mouse

Human;Mouse;Rat


Human;Rat;Mouse



Human;MouseHuman;Mouse Avian;Human;Mouse;Mink;Rat

Human

Human

Human;Mouse

Chicken;Human;Mouse

Human;Mouse


100 µL

100 µL100 µL

100 µg

100 µg100 µL

100 µg

200 µL

200 µg

50 µL

250 µg

200 µg100 µL

100 µg

100 µg100 µg

200 µg200 µg

100 µg100 µL

200 µg200 µg200 µg

100 µl

100 µL

100 µg

100 µL

100 µg

100 µg200 µL

29

mTOR Signaling作为对营养和生长因子的反应，PI3激酶/Akt/mTOR通路会被活化并刺激细胞进行增殖和一系列基因的翻译。这个信号通路的失调

会导致多种人类肿瘤。据报道，发现超过30％的实体瘤中包含PI3-K的催化单位发生了突变，致使酶活、细胞增殖、细胞侵袭、

细胞转移等都有所提高。正常情况下，TSC-1和TSC-2形成二聚体复合物，是Rheb的抑制剂，而Rheb是mTOR活化所必需的刺激蛋

白，因此TSC-1/TSC-2在正常情况下抑制mTOR的功能。当Akt活化后，它可磷酸化TSC-2的Ser939和Thr1462，抑制了TSC-1/TSC-2复合

物的形成，从而解除了对Rheb的抑制作用，使得mTOR被激活。活化的mTOR通过磷酸化蛋白翻译过程中的某些因子来参与多项细

胞功能，其中最主要的是4EBP1和P70S6K。

09-345AB606004-1521ABE62105-1527

07-1408

07-1331ABC47709-104

09-230

ABS54407-53207-88607-1586

Anti-Anti-phospho-mTOR (Thr2446) AntibodyAnti-BBIP10 AntibodyAnti-RPA1 p70 Antibody, clone RPA9, 1-30Anti-EIF4A2 AntibodyAnti-eIF4A3 Antibody, clone 3F1

Anti-Glucose Regulating Protein 94 (GRP94) AntibodyAnti-phospho-Rictor (Ser1219) AntibodyAnti-YY1AP1 AntibodyAnti-phospho-Raptor (Ser722) Antibody

Anti-phospho-Raptor (Thr706) Antibody

Anti-phospho-Raptor Antibody (Ser696)Anti-phospho-PKR (Thr446) AntibodyAnti-phospho-PKR (Thr451) AntibodyAnti-HIF-1-alpha Antibody, hydroxyproline (Pro564)

Western BlottingWestern BlottingWestern Blotting;ImmunocytochemistryWestern Blotting;ImmunohistochemistryWestern Blotting;Immunoprecipitation;ImmunocytochemistryWestern Blotting;Immunocytochemistry

Western BlottingWestern Blotting;ImmunohistochemistryDot Blot;Western Blotting

Western Blotting;Dot Blot

Western BlottingWestern BlottingWestern BlottingWestern Blotting;Peptide Inhibition Assay

Human;Mouse;RatHuman;MouseHumanHumanHuman;Mouse

Human;Monkey;Rat

Human;RatHuman;Mouse;RatHuman;Mouse;Rat;Bovine;Canine;Goat;Chicken;HorseHuman;Mouse;Rat;Canine;Bovine;HorseHumanHumanHuman;MouseHuman

100 µL100 µg100 µg150 µL100 µg

100 µL

100 µg100 µg100 µg

100 µg

200 µL100 µg100 µL100 µL

DescriptionCat. No. Key Applications Species Reactivity Qty/Pk

抗体

30

05-123805-151805-1516

05-1214ABS772

MABE102

ABS97ABS625

07-133109-104

09-10709-230

ABS54405-70605-1214MAB126

05-1134

AB5312

AB5220

AB9040

AB1554

AB1526

ABS625

AB5156-50UL

AB5785AB5787AB5795

MAB13170

07-1015

07-1016

07-53207-886AB10181

Anti-PARN Antibody, clone 7A7.1Anti-hnRNP L Antibody, clone 4D11Anti-hnRNP U Antibody, clone 3G6

Anti-FXR2 Antibody, clone 2C8.2Anti-DDX50 Antibody

Anti-FIH-1 Antibody, clone FIH 162c

Anti-KEAP1 AntibodyAnti-PDIA3 Antibody

Anti-phospho-Rictor (Ser1219) AntibodyAnti-phospho-Raptor (Ser722) Antibody

Anti-phospho-Raptor (Ser877) AntibodyAnti-phospho-Raptor (Thr706) Antibody

Anti-phospho-Raptor Antibody (Ser696)Anti-CRMP1 Antibody, clone Y21Anti-FXR2 Antibody, clone 2C8.2Anti-Epidermal Growth Factor Antibody, clone 144-8Anti-Fibroblast Growth Factor Receptor 1Antibody, clone VBS1Anti-Growth Associated Protein 43 Antibody

Anti-Growth Associated Protein-43 (GAP-43) Antibody

Anti-Nerve Growth Factor Antibody, pro

Anti-Nerve Growth Factor ReceptorAntibody, p75Anti-Nerve Growth Factor-β Antibody

Anti-PDIA3 Antibody

Anti-Calcium Channel Antibody, Voltage Gated α 1CAnti-Calcium Channel β 1 AntibodyAnti-Calcium Channel β 2 Antibody, near CTAnti-Calcium Channel γ 4 Antibody

Anti-Cav1.2 calcium channel Antibody,clone L57/46Anti-phospho-Upf1 (Ser1100) Antibody

Anti-phospho-Upf1 (Ser1127) Antibody

Anti-phospho-PKR (Thr446) AntibodyAnti-phospho-PKR (Thr451) AntibodyAnti-Pumilio-2 (PUM2) Antibody

Western Blotting;ImmunocytochemistryWestern Blotting;ImmunoprecipitationWestern Blotting;Immunoprecipitation;Immunocytochemistry;ELISAWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunocytochemistryWestern BlottingDot Blot;Western Blotting

Western BlottingWestern Blotting;Dot Blot

Western BlottingWestern BlottingWestern Blotting;ImmunocytochemistryImmunohistochemistry;Immunoprecipitation

Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);ELISAImmunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western BlottingELISA;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;Western Blotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry(Paraffin);ImmunoprecipitationELISA;Western Blotting;Immunohistochemistry;Affects FunctionWestern Blotting;Immunohistochemistry;ImmunocytochemistryImmunohistochemistry;Immunoprecipitation;Western BlottingWestern BlottingWestern BlottingWestern Blotting

Western Blotting;Immunohistochemistry



Western BlottingWestern BlottingImmunocytochemistry;Immunohistochemistry;Western Blotting

HumanHumanHuman

Human;Rat;MouseHuman

Human


Human;RatHuman;Mouse;Rat;Bovine;Canine;Goat;Chicken;HorseHuman;Primate;MonkeyHuman;Mouse;Rat;Canine;Bovine;HorseHumanHumanHuman;Rat;MouseHuman;Mouse

Human;Mouse;Rat;Chicken;Bovine

Bovine;Human;Mouse;Monkey;Rat;Feline

Human;Mouse;Rat;Chicken;Bovine

Human;Mouse;Rat

Human;Mouse;Rat

Chicken;Human;Mouse;Rat

Human

Human;Mouse;Rat;Rabbit

HumanHuman;Mouse;RatHuman;Mouse;Rat

Mouse;Human;Rat;Rabbit

Xenopus;Human;Bovine;Horse;Chicken;Mouse;RatHuman;Xenopus;Bovine;Mouse;Rat;ChickenHumanHuman;MouseHuman;Mouse;Chimpanzee;Rhesus Macaque;Canine

100 µg100 µL100 µg

100 µg100 µL

100 µg

100 µg100 µL

100 µg100 µg

100 µg100 µg

200 µL100 µL100 µg200 µL

100 µg

100 µL

100 µL

100 µL

100 µL

250 µL

100 µL

50 µL

100 µg100 µg100 µg

100 µg

100 µg

100 µg

100 µg100 µL100 µg


抗体

AB5605AB5830MAB3560

AB9890

07-520

MAB5310AB3282

MABN527ABS97MABC71307-685

MABN457

06-984

ABC391AB5380AB5382

AB16301AB553205-233

16-226

16-207

16-310

07-61007-35707-56107-562AB5484MAB5328

ABC245MABC684

AB10346AB5482AB9328

Anti-4-Hydroxynonenal AntibodyAnti-8-Hydroxydeoxyguanosine AntibodyAnti-8-Oxoguanine Antibody, clone 483.15

Anti-AGE (Advanced Glycated End-products) AntibodyAnti-eNOS/NOS III Antibody, CT

Anti-Glutathione Antibody, clone D8Anti-Glutathione-S-Transferase Antibody, S. japonicum formAnti-iNOS/NOS II, clone 13F5.1 AntibodyAnti-KEAP1 AntibodyAnti-KEAP1, clone 7G4B10 AntibodyAnti-Lactotransferrin Antibody

Anti-Mitochondrial dicarboxylate carrier Antibody, clone 1F5.1Anti-Mn-SOD Antibody

Anti-NDUFB7 AntibodyAnti-Nitric Oxide Synthase I AntibodyAnti-Nitric Oxide Synthase II Antibody

Anti-Nitric Oxide Synthase III AntibodyAnti-Nitrotyrosine AntibodyAnti-Nitrotyrosine Antibody, clone 1A6

Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor® 488 conjugateAnti-Nitrotyrosine Antibody, clone 1A6, HRP conjugateAnti-Nitrotyrosine Magnetic Bead Conjugate AntibodyAnti-Peroxiredoxin 2 AntibodyAnti-phospho-eNOS/NOS III (Ser116) AntibodyAnti-phospho-eNOS/NOS III (Ser617) AntibodyAnti-phospho-eNOS/NOS III (Ser635) AntibodyAnti-RAGE AntibodyAnti-RAGE Antibody, clone DD/A11 or A11

Anti-REDD1 AntibodyAnti-SOD1 Antibody, clone 6F5

Anti-SOD2 AntibodyAnti-Superoxide Dismutase 1 AntibodyAnti-Thioredoxin 1 Antibody

ELISA;Western BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)ELISA;Western Blotting

Western Blotting

Western BlottingELISA;Western Blotting

Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryImmunohistochemistry;Flow Cytometry;ELISAImmunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin)Western Blotting;Immunohistochemistry

Immunohistochemistry;Immunohistochemistry (Paraffin);Western BlottingWestern Blotting;ImmunohistochemistryImmunohistochemistry;Western BlottingImmunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;ImmunocytochemistryELISA;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingFlow Cytometry;Western Blotting


Immunoprecipitation

Western Blotting;ImmunocytochemistryWestern BlottingWestern BlottingWestern BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunocytochemistry;Flow CytometryWestern BlottingWestern BlottingWestern Blotting

HumanHumanHuman;Rat;Mouse;Monkey

Human

Human;Rat;Bovine;Mouse;Canine;Guinea Pig;PigEukaryoteVertebrates;Schistosoma japonicum

HumanHuman;Mouse;RatHumanHuman;Mouse;Rat;Hamster;Monkey

Mouse;Rat

Human;Rat;Mouse;Bovine

Human;MouseHuman;Mouse;Monkey;Rat;SheepMouse;Rat

HumanAllVertebrates

Vertebrates

All

All

HumanBovine;HumanBovineBovineHumanBovine;Human;Mouse


Human;Rat;MouseMouse;RatMouse;Rat

200 µL200 µL100 µL

200 µL

200 µg

100 µg500 µg

100 µL100 µg100 µg100 µL

100 µg

200 µg

150 µL50 µL50 µL

100 µg100 µL100 µg

100 µg

50 µL

200 µL

50 µL100 µL200 µL200 µL200 µL100 µg

100 µg100 µL

50 µL50 µL100 µL

31

Milliplex kit产品描述货号

抑制剂

124018-1MGCN124033-10MGCN171260-1MGCN203695-10MGCN234501-5MGCN371963-1MGCN475988-5MGCN475990-5MGCN521276-5MGCN528100-1MGCN528113-1MGCN529584-5MGCN553210-1MGCN559273-10MGCN565835-10MGCN565840-10MGCN662005-1MGCN

Akt Inhibitor VIII, Isozyme-SelectiveAkt Inhibitor XVI, AT7867AMPK Inhibitor, Compound CbpV(phen)Compound 401H-89, DiHClmTOR Inhibitor III, PP242mTOR Inhibitor IV, Ku-63794PDK1 Inhibitor IIPI-103PI 3-Kbeta Inhibitor VI, TGX-221PTK/PI 3-K/mTOR Inhibitor, PP121RapamycinS6K1 Inhibitor, PF-4708671SHIP1 Inhibitor, 3ACSHIP2 Inhibitor, AS1938909U0126 1,4-DIAMINO-2,3-DICYANO-1,4-BIS...

Akt/mTOR(Phosphoprotein)-11Plex(Cat.No.48-611MAG)AB2


Akt/PKB

GSK3α

GSK3β

IGF1R

IR

IRS1

mTOR

p7OS6 Kinase

PTEN

RPS6

TSC2

(Ser473)

(Ser21)

(Ser9)

(Tyr1135/1136)

(Tyr1162/1163)

(Ser636)

(Ser2448)

(Thr389/412)

(Ser380)

(Ser235/236)

(Ser939)

H,M,R

H,M,R

H,M,R

H,M

H

H,R

H,M

H,M,R

H,M,R

H,M,R

H,M,R

Akt/mTOR(Total)-11Plex(Cat.No.48-612MAG)AB2


Akt/PKB

GSK3α

GSK3β

IGF1R

IR

IRS1

mTOR

p7OS6 Kinase

PTEN

RPS6

TSC2

H,M,R

H,M,R

H,M,R

H,M,R

H,R

H,M,R

H,M,R

H,M,R

H,M,R

H,M,R

H,M,R

mTOR Phospho/Total -2 Plex(Cat.No.48-625MAG)AB2


mTOR

mTOR

H,M,R

H,M

(Ser2448)

32

Hayden, M.S., and Ghosh, S. 2012. Genes Dev. 26, 203.Ben-Neriah, Y., and Karin, M. 2011. Nat. Immunol. 12, 715.Oeckinghaus. A., et al. 2011. Nat. Immunol. 8, 695Karin, M., and Greten, F.R. 2005. Nat. Rev. Immunol. 5, 749

参考文献Bouwmeester, T., et al. 2004. Nat. Cell. Biol. 6, 97.Chen, L.F., and Greene, W.C. 2004. Nat. Rev. Mol. Cell Biol. 5, 392。Karin. M., et al. 2004. Nat. Rev. Drug Disc. 3, 17

NF-κB SignalingNF-κB在细胞因子诱导的基因表达中起关键性的调控作用，它调控的基因编码急性期反应蛋白、细胞因子、细胞粘附分子、免疫调节分子、病毒瘤基因、生长因子、转录和生长调控因子等。通过调控多种基因的表达，NF-κB参与免疫反应、炎症反应、细胞凋亡、肿瘤发生等多种生物进程。

NF-κB主要有两种活化方式：一、经典的NF-κB活化途径：静止状态时，NF-κB以无活性的潜在状态存在于细胞浆中,它与抑制因子IkB结合组成一个三聚体p50-p65-IkB；在IkBs激酶（IKK）催化IkBs的两个保守的丝氨酸残基磷酸化；IkBs在SCF-E3泛素化酶复合体的催化作用下多泛素化而被蛋白酶降解；活化的NF-κB转位到核内同与其相关的DNA基序结合以诱导靶基因转录；活化的NF-κB快速诱导编码自身抑制剂IkBa的基因的转录；新合成的IkBa进入细胞核，使NF-κB与DNA解离并排出细胞核，等待重新激活。二、非经典的NF-κB活化途径：广泛的IkBs家族也包括P50和P52前体形式的NF-κB1和NF-κB2，分别是P105和P100。除了P50和P52序列外，这些前体还包括IkB样的锚蛋白区，它抑制与其相关的NF-κB亚单位的活性。从前体产生P50和P52的过程还没有被人完全的理解，但它需要翻译时和翻译后的蛋白酶加工处理过程。在翻译的同时就会组成性的产生约等量的P50和P105，虽然这时P50还没有加工完成。P52的产生主要但不完全是由于信号诱导的P100加工完成的。不像是IkBa、IkBβ、IkBε的降解，信号诱导的磷酸化及加工P100成P52不需要经典的IKK-γ依赖的信号途径。IKK-a和NF-κB诱导激酶（NIK）是必不可少的，但IKK-β和IKK-γ是不需要的。

Phosphorylation

Ubiquitin


Nucleus

Bacterial Antigens

Growth Factors

Growth FactorReceptors

TNFα

Proteosome

TNFR1 IL-1R

IL-1

• PDK/Akt/Flt Dual

PathwayInhibitor

• Interluekin-1 Receptor-AssociatedKinase-1/4 Inhibitor

• PD 98059

• (5Z)-7-Oxozeaenol, Curvularia sp.

• IKK Inhibitor VII

• IKK Inhibitor VII

• NEMO-Binding Domain BindingPeptide, Cell Permeable • BAY 11-7082

• Tpl2 Kinase Inhibitor

• Histone Acetyltransferase Inhibitor II• Trichostatin A,Streptomyces sp.

• JNK Inhibitor II

• MG-132

• BAY 11-7082

p52RelB

NF-κB

p52p54

NF-κBNF-κB

p50c-Rel

NF-κB

p50c-Rel

NF-κB

IκBs

HDAC3

p50

p50

p50Bcl3

p50

p105p105

ABIN-2Tpl2

LPS

TRADDTRAF2

p300

CBP

TRAF6

TRAF6

MALPPGN

MyD88TOLLIPMyD88

PI 3-K

PDK-1

ASK1

MKK4,7

JNKsAkt/PKB

IKK-γ

MEKKs

Tpl2

MEKK

ERK

PKR PKR

IRAKIRAK

IRAK

TAK1

IKK-α IKK-β

JNK PathwayERK

Signaling

Tpl2Degradation

Virus

IκB Degradation

Nuclear Import

SCF/β-TrCP

IκBs

RelBp52NF-κB

IκBs

IκBs

p50c-Rel

NF-κB

IκBs p52

p65

NF-κB

κ-BRas

IκBs p52

p65

NF-κB

IκBs p52

p65

NF-κB

PACT

Nuclear Export

AcetylationDeacetylation

33


BAY 11-7082 1 mg 196870

IKK Inhibitor VII 1 mg 401486

Interleukin 1- Receptor-Associated-Kinase-1/4 Inhibitor 5 mg 407601

Histone Acetyltransferase Inhibitor II 10 mg 382110

MG-132 5 mg 474790

NEMO-Binding Domain-Binding Peptide, Cell-Permeable 500 µg 480025

NF-κB SN50, Cell-Permeable Inhibitor Peptide 500 µg 481480

PD 98059 5 mg 513000

PDK1/Akt/Flt Dual Pathway Inhibitor 5 mg 521275

Trichostatin A, Streptomyces sp. 1 mg 647925

TIRAP Inhibitor Peptide, Cell-Permeable 1 mg 613570

Tpl2 Kinase Inhibitor 1 mg 616373

(5Z)-7-Oxozeaenol, Curvularia sp. 1 mg 499610

JNK inhibitor II 5 mg 420119

Anhydrous DMSO 15 mL

InhibitorSelect™ NF-κB Signaling Pathway Inhibitor Panel (Catalogue No. 481487)

The Inhibitor panel includes:

NF-κB抗体Description Species Applications Qty Catalogue No.

Anti-IκB-α Antibody Human, Mouse IB, IP 200 µg 06-494

Anti-IκBα Human, Mouse, Rat ELISA, IB, IP, (IH)P 100 µL 07-1483

Human, Monkey, Mouse IB 100 µg 05-536

Anti-IKKα Mouse mAb (14A231) Human, Monkey, Mouse IB 100 µg OP133

Anti-IKKα Most mammalian species IB, IP 150 µg 07-1007

Anti-IKKα, clone Y463, Rabbit Monoclonal Human, Mouse, Rat IB, IC, IP, FC, IH(P) 100 µL 04-365

Anti-IKKβ, Mouse Monoclonal (10AG2) Human IB 100 µg OP134

Anti-IKKβ, Mouse Monoclonal (10AG2) Human IB 100 µg OP134

Anti-IKKβ, clone 10AG2 Human, Mouse, Rat IB 100 µg 05-535

Anti-IKKβ Human, Mouse IB, IH(P) 100 µg 07-1479

Anti-IKKβ Most mammalian species IB 150 µg 07-1008

Anti-IKKβ, clone Y466, Rabbit Monoclonal Human, Mouse FC, IB, IH(P), IP 100 µL 04-366

Anti-IKKγ Human, Mouse IB 100 µg 05-631

Anti-IKKε Antibody Human, Mouse IB 200 µg 07-580

Anti-phospho-IKKε (Ser172) Human, Mouse, Rat IB 100 µL 06-1340

Anti- NF-κB p50, clone E381 Human, Mouse, Rat FC, IB, IC, IH, IH(P) 100 µL 04-234

Anti- NF-κB p50 Human, Mouse IB, IP, ChiP 150 µL 06-886

Anti-NF-κB p52 Human IB, IP 100 µL 06-413

Anti-NF-κB p65 Human, Mouse, Rat IB, IP 100 µL ABE136

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit Human ELISA, EMSA, IB, IP 100 µL PC138

Anti-NF-κB, p65 Subunit, Active Subunit, clone 12H11

Human, Mouse, Rabbit, Rat EMSA, FC, IB, IC, IH(P) 100 µg MAB3026

Anti-NF-κB p52 Human EMSA, IB, IP 200 µg 05-361

Anti-NF-κB p65 Human, Mouse, Rat IB, IP 100 µg ABE347

Anti-NF-κB p65, C-Terminus Human, Mouse, Rat EMSA, IB, IP 100 µg 06-418

Anti-NF-κB, p65, clone 1G10.2 Bovine, Human, Mouse, Rat IB, IC, IF 200 µg 05-1469

Anti-NF-κB p65, Phospho-speci�c (Ser276) Human ELISA, IB 100 µL AB3375

Anti-NF-κB p65, clone EP2161Y Human FC, IB, IC 100 µL 04-1008

Anti-Phospho-NF-κB p65 (Ser536), clone EP2294Y

Human IB, IP 100 µL 04-1000

参考文献:Recruitment of IκBα to the hes1 promoter is associated with transcriptional repression. PNAS. doi: 10.1073/pnas.0404429101

COMMD proteins, a novel family of structural and functional homologs of MURR1. JBC. doi: 10.1074/jbc.M501928200

参考文献:Nonredundant and complementary functions of TRAF2 and TRAF3 in a ubiquitination cascade that activates NIK-dependent alternative NF-B signaling.Nature Immunology 9, 1364 - 1370 ,2008

zVAD-induced necroptosis in L929 cells depends on autocrine production of TNFα mediated by the PKC–MAPKs–AP-1 pathway.Cell Death and Differentiation (2011) 18, 26–37

The synthetic epoxyquinoids jesterone dimer and epoxyquinone A monomer induce apoptosis and inhibit REL (human c-Rel) DNA binding in an IκBα-de�cient diffuse large B-cell lymphoma cell line.Cancer Letters. doi:10.1016/j.canlet.2005.10.004

参考文献:Anaplasma phagocytophilum dihydrolipoamide dehydrogenase 1 affects host-derived immunopathology during microbial colonization. Chen, Gang, et al.Infect. Immun., 80: 3194-205，2012

Human herpes simplex viruses in benign and malignant thyroid tumours. Jensen K, Patel A, Larin A, Hoperia V, Saji M, Bauer A, Yim K, Hemming V, Vasko V. J Pathol 221 193-200. 2010

参考文献:Nuclear factor-kappaB induced by doxorubicin is de�cient in phosphorylation and acetylation and represses nuclear factor-kappaB-dependent transcription in cancer cells. Wai Chi Ho, Kathleen M Dickson, Philip A Barker. Cancer research,65:4273-81, 2005

Ineffectiveness of histone deacetylase inhibitors to induce apoptosis involves the transcriptional activation of NF-kappa B through the Akt pathway. Mayo, M. W., et al J Biol Chem, 278:18980-9, 2003


34

AB9730

AB9746

AB9732

MABC749

ABE1007AB10410

AB1579407-53507-822

05-797R

05-1152

07-507

MABE329

MABS1190

06-225

MABS15006-47806-659

06-828

05-107607-1333

05-1072

05-1028

MABS64

ABS29

09-272

ABS29

05-747

07-64107-852

07-641













Western Blotting










Western Blotting


Western Blotting

Western Blotting




HumanHuman;Horse


Human;Mouse;Rat



Human

Human

Avian;Human;Mouse

RatHumanHuman;Mouse

Human;Mouse;Rat


Human;Mouse;Rat

Human;Mouse




Human;Mouse;Rat

100 µL

100 µL

100 µL

100 µL

100 µg100 µg

100 µL100 µg100 µL

100 µL

100 µg

200 µL

100 µg

100 µg

200 µL

100 µg250 µg100 µL

100 µL

100 µg100 µg

100 µg

100 µg

100 µg

100 µg

100 µL

100 µg

100 µL

200 µg100 µL

200 µg

Applications:ChIP: Chromatin ImmunoprecipitationDB: Dot BlottingEMSA: Electrophoretic Mobility Supershift Assay ELISA: Enzyme-linked Immunosorbent Assay FC: Flow Cytometry IB: Immunoblotting IC: Immunocytochemistry IH: ImmunohistochemistryIP: ImmunoprecipitationPIA: Peptide Inhibition Assay

Description Species Applications Qty Catalogue No.

Anti-NF-κB p65, C-Terminus Human, Mouse, Rat EMSA, IB, IP 100 µg 06-418

Anti-NF-κB, p65, clone 1G10.2 Bovine, Human, Mouse, Rat IB, IC, IF 200 µg 05-1469

Anti-NF-κB p65, Phospho-speci�c (Ser276) Human ELISA, IB 100 µL AB3375

Anti-NF-κB p65, clone EP2161Y Human FC, IB, IC 100 µL 04-1008

Anti-Phospho-NF-κB p65 (Ser536), clone EP2294Y

Human IB, IP 100 µL 04-1000

Anti-Phospho-NF-κB (p100) (Ser707) Human DB, IB, PIA 200 µL 07-1829

Anti-NIK, C-terminus Human IB 100 µg AB16528

Anti-RelB Human IB, IC, IF 200 µL 07-1332

Anti-RelB Human, Mouse, Rat, Rhesus Monkey

IB, IH(P) 100 µg 06-1105

Anti-c-Rel (NF-κB) Human ELISA, EMSA, IB 100 µg 09-040

Anti-TBK1, clone AOW9 Canine, Chimpanzee, Human, Mouse

IB, IP 100 µL 04-856

Anti-TBK1/NAK (N-term), clone EP611Y Human, Mouse, Rat ICC, IB, IH(P),IP 100 µL 04-387

Anti-TBK1 Mouse (108A429) Human IB 100 µg AM76

Anti-Phospho-TBK1 (Ser172) Human, Mouse, Rat IB 100 µL 07-2192



NF-κB抑制剂Description Comments Qty/Pk Catalogue No.

Andrographolide An irreversible blocker of NF-κB and AP-1 (IC50 ≤ 15 µM) activation with anti-in�ammatory, anti-apoptotic properties.

50 mg 172060

Aurothiomalate Blocks NF-κB activation and PKCι-dependent Rac1 signaling. Modi�es metal sensitive cysteine residues of IKK and PKCι, and inhibits the activity of IKK (IC50 = 10.9 µM).

50 mg 189401

Bay 11-7082 Selective, irreversible inhibitor of TNFα-inducible phosphorylation of IκB-α (IC50 = 10 µM) without affecting the constitutive IκB-α phosphorylation. Reduces the nuclear translocation of NF-κB.

10 mg 196870

InSolution™ BAY 11-7082 A 100 mM (10 mg/483 µL) solution of BAY 11-7082 (Catalogue No. 196870) in DMSO.

10 mg 196871

Bay 11-7085 Exhibits biological properties similar to that of BAY 11-7082 (Catalogue No. 196870). BAY 11-7085 has also been shown to have potent anti-in�ammatory properties in vivo.

10 mg 196872

CAPE (Caffeic Acid Phenethyl Ester, Synthetic) A cell-permeable active component of propolis from honeybee hives with antiviral, anti-in�ammatory, and immunomodulatory properties. Has been shown to act as a potent and speci�c inhibitor of NF-κB activation.

25 mg 211200

(E)-Capsaicin An active constituent of cayenne pepper with excitatory and desensitizing effects on a subset of primary afferent sensory neurons. Inhibits NF-κB activation by TNF.

100 mg 211275

EF24 (3,5-bis-(2-Fluorobenzylidene)-piperidin-4-one)

A cell-permeable analog of Curcumin that acts as a potent inhibitor of IKKβ inhibitor (IC50 = 1.9 µM) and is shown to block and NF-κB nuclear translocation (IC50 = 1.3 µM).

10 mg 324510

Gliotoxin, Gladiocladium �mbriatum An immunosuppressive secondary metabolite produced by several pathogenic fungi. Speci�cally inhibits NF-κB in B and T cells in nanomolar concentrations.

1 mg 371715

Hypoestoxide, Hypoestes rosea A naturally occurring, cell-permeable diterpene that acts as a selective and direct inhibitor of IκB kinase (IC50 = 24 µM) in TNF-α stimulated HeLa cells thereby prevents NF-κB activation.

1 mg 401006

IκB Kinase Inactive Control Peptide, Cell-Permeable (Ac-AVALLPAVLLALLAPDDRHDAGLDAMKDE-NH2)

An inactive control for IκB Kinase Inhibitor Peptide (Catalogue No. 401477). A 14-amino acid peptide corresponding to the mutated recognition sequence of IκB (Ser32 → Ala and Ser36 → Ala), linked to the hydrophobic region of the �broblast growth factor signal peptide.

1 mg 401478

IκB Kinase Inhibitor Peptide, Cell-Permeable (Ac-AAVALLPAVLLALLAPDDRHDSGLDSMKDE-NH2)

A 14-amino acid peptide corresponding to the active IκB phosphorylation recognition sequence, linked to the hydrophobic region of the �broblast growth factor signal peptide to aid in cellular delivery. Speci�cally inhibits LPS-induced IκB degradation by IκB kinases (IKK) in RAW 264.7 cells (<50 µg/mL) and prevents NF-κB activation.

1 mg 401477

35

07-853

07-69136-010

ABS94

ABC3607-865

07-250

05-577

05-421

07-604

05-389

07-45307-814

05-516

07-147706-918

06-74807-175

05-986MABS771

06-11807-33705-184

04-889

07-791

05-677

07-909

MAB4369

06-133606-1425




Anti-Rac2 Antibody





Western Blotting










Western Blotting





Human;Mouse;Rat




Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast (<italic>S. cerevisiae</italic>)Human;Mouse

Human;Mouse;Rat


Human;Rat;Mouse



Human;MouseHuman;MouseAvian;Human;Mouse;Mink;RatHuman

Human

Human;Mouse

Chicken;Human;Mouse

Human;Mouse


100 µL

100 µL100 µL

100 µg

100 µg100 µL

100 µg

200 µL

200 µg

50 µL

250 µg

200 µg100 µL

100 µg

100 µg100 µg

200 µg200 µg

100 µg100 µL

200 µg200 µg200 µg

100 µl

100 µL

100 µg

100 µL

100 µg

100 µg200 µL

Description Comments Qty/Pk Catalogue No.

IκB Kinase Inhibitor Peptide, Cell-Permeable (Ac-AAVALLPAVLLALLAPDDRHDSGLDSMKDE-NH2)

A 14-amino acid peptide corresponding to the active IκB phosphorylation recognition sequence, linked to the hydrophobic region of the �broblast growth factor signal peptide to aid in cellular delivery. Speci�cally inhibits LPS-induced IκB degradation by IκB kinases (IKK) in RAW 264.7 cells (<50 µg/mL) and prevents NF-κB activation.

1 mg 401477

IKK Inhibitor II, Wedelolactone A cell-permeable, selective, and irreversible inhibitor of IKKα and IKKβ (IC50 < 10 µM). Inhibits NF-κB -mediated gene transcription in cells by blocking the phosphorylation and degradation of IκBα.

1 mg 401474

IKK Inhibitor III, BMS-345541 (4-(2’-Aminoethyl)amino-1,8-dimethylimidazo[1,2-a]

quinoxaline)

A cell-permeable, potent, selective, and allosteric site-binding inhibitor of IKK-2 (IC50 ~300 nM) that displays anti-in�ammatory properties. Exhibits ~10-fold greater selectivity for IKK-2 over IKK-1 (IC50 ~4.0 µM).

1 mg 401480

IKK Inhibitor VII A cell-permeable benzamido-pyrimidine compound that acts as a potent, selective, and ATP-competitive inhibitor of IKK (IC50 = 40 nM, 70 nM, and 200 nM for IKK-2, IKK complex, and IKK-1, respectively).

1 mg 401486

IKK Inhibitor X (N-(6-Chloro-9H-β-carbolin-8-yl)nicotinamide)

A cell-permeable β-carboline that acts as a potent, ATP-competitive, and reversible inhibitor of IKK (IC50 = 88 nM) with selectivity over 14 other commonly studied kinases (IC50 > 100 µM), including NF-κB inducing kinase.

5 mg 401489

IKK Inhibitor XII A cell-permeable amino-diarylbenzamide compound that acts as a potent ATP site-targeting inhibitor against IKK-1 and IKK-2 (pIC50 = 6.4 and 7.0, respectively).

5 mg 401491

IKK Inhibitor XIII, 17-AJB A cell-permeable, irreversible inhibitor against IKK-β activity (IC50 = 300 nM). 1 mg 401493

IKK-2 Inhibitor IV ([5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide,

TPCA-1)

A cell-permeable, reversible, ATP-competitive, and potent inhibitor of IKK-2 (IC50 = 18 nM) that exhibits selectivity over IKK-1, JNK, and p38 MAPK.

500 µg 401481

InSolution™ IKK-2 Inhibitor IV A 10 mM (500 µg/179 µL) solution of IKK-2 Inhibitor IV (Catalogue No. 401481) in DMSO.

500 µg 401484

IKK-2 Inhibitor V (N-(3,5-Bis-tri�uoromethylphenyl)-5-chloro-2-

hydroxybenzamide, IMD-0354)

A cell-permeable IKK-2 inhibitor that selectively blocks IκBα phosphorylation (IC50 ~250 nM) and prevents the induction of NF-κB p65 nuclear translocation.

5 mg 401482

IKK-2 Inhibitor VI (5-Phenyl-2-ureido)thiophene-3-carboxamide)

An ureido-thiophenecarboxamide compound that acts as a potent, cell-permeable, reversible, and ATP-competitive inhibitor of IKK-2 (IC50 = 13 nM).

1 mg 401483

IKK-2 Inhibitor VIII (2-Amino-6-(2-(cyclopropylmethoxy)-6-hydroxyphenyl)-4-(4-

piperidinyl)-3-pyridinecarbonitrile, ACHP)

A cell-permeable piperidinyl-pyridine compound that acts as a selective inhibitor of IKK-2 (IC50 = 8.5 and 250 nM for IKK-2 and IKK-1, respectively).

1 mg 401487

IKK-2 Inhibitor XI (5-Phenyl-3-ureido)-thiophene-2-carboxamide

A cell-permeable thiophenecarboxamide-ureido compound that acts as a potent, ATP-binding pocket-targeting inhibitor of IKK-2 (IC50 = 25 nM).

5 mg 401490

IKK-2 Inhibitor, SC-514 A cell-permeable, potent, reversible, ATP-competitive and highly selective inhibitor of IKK-2 (IC50 ~3-12 µM for IKK-2 homodimer, IKK-1/IKK-2 heterodimer, and IKK-2).

1 mg 401479

InSolution™ IKK-2 Inhibitor, SC-514 A 25 mM (1 mg/178 µL) solution of IKK-2 Inhibitor, SC-514 (Catalogue No. 401479) in DMSO.

1 mg 401485

IKK-3 Inhibitor IX (5-(5,6-Dimethoxybenzimidazol-1-yl)-3-(2-methanesulfonyl-

benzyloxy)-thiophene-2-carbonitrile

A thiophenecarbonitrile compound that acts as a potent ATP-competitive inhibitor of IKK-3 (IKK-ε, IC50 = 40 nM).

5 mg 401488

Isohelenin, Inula sp. A cell-permeable sesquiterpene lactone that acts as a highly speci�c, potent, and irreversible inhibitor of NF-κB activation by preventing IκBα degradation. Does not affect the DNA binding activity of activated NF-κB.

1 mg 416157

NEMO-Binding Domain Binding Peptide, Cell-Permeable (DRQIKIWFQNRRMKWKKTALDWSWLQTE)

A cell-permeable Antennapedia-NBD (wild type) fusion peptide that blocks the association of NEMO with the IKK complex and prevents NF-κB activation.

500 µg 480025

NBD-Binding Peptide II, Cell Permeable, CTP-NBD (CTP; YGRRARRRARR; NBD; TALDWSWLQTE)

A cell-permeable cytoplasmic transduction peptide (CTP) fused to the NEMO binding domain (NBD). Inhibits TNFα-stimulated NF-κB activity (IC50 ~ 50 µM in HCT116 cells). Interferes with the formation of active IKK complex and thereby prevents IκBα phosphorylation and its subsequent proteasomal degradation.

5 mg 480026

NEMO-Binding Domain Binding Peptide, Cell-Permeable, Negative Control (DRQIKIWFQNRRMKWKK-TALDASALQTE)

A cell-permeable, Antennapedia-NBD mutated (Trp739 → Ala and Trp741 → Ala) fusion peptide analog of NEMO-Binding Domain Binding peptide (Catalogue No. 480025) that serves as a negative control.

500 µg 480030

NEMO NOA Pepotide, Cell-Permeable, A-UBI (NEMO Ubiquitin Binding Domain Inhibitor)

A cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site that attenuates NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex).

2 mg 481418

NF-κB Activation Inhibitor 6-Amino-4-(4-phenoxyphenylethylamino)quinazoline

A cell-permeable quinazoline compound that acts as a potent inhibitor of NF-κB transcriptional activation (IC50 = 11 nM in Jurkat cells).

1 mg 481406

36

NF-κB Activation Inhibitor V, 5HPP-33 (5-Hydroxy-(2,6-diisopropylphenyl)-1H-isoindole-1,3-dione)

A cell-permeable N-phenylpthalimide compound that inhibits IL-1-induced NF-κB translocation in HeLa cells (IC50 = 530 nM). Effectively suppresses the proliferation of various cancer cells, including several Paclitaxel-resistant lines.

25 mg 100066

NF-κB Activation Inhibitor VI, BOT-64 (6,6-Dimethyl-2-(phenylimino)-6,7-dihydro-5H-benzo[1,3]

oxathiol-4-one)

A cell-permeable benzoxathiole compound that acts as an IKK-2 inhibitor, presumably by targeting the Ser177 and/or Ser181 residues in the kinase's activation loop domain.

5 mg 481414

NF-κB Activation Inhibitor VII, CID-2858522 (1-(3,5-di-tert-butyl-4-hydroxyphenyl)-2-(2-(3-

hydroxypropylamino)-5,6-dimethyl-1H-benzo[d]imidazol-1-yl)

ethanone;)

A cell-permeable, selective inhibitor of PMA-induced NF-κB activation PKC pathway activators such as PMA (IC50 = 70 nM).

10 mg 480457

NF-κB Activation Inhibitor VIII, EVP4593 (N4-(4-phenoxyphenethyl)quinazoline-4,6-diamine,

SOC Inhibitor)

A cell-permeable quinazoline derivative that blocks PMA/PHA-induced NF-κB activation (IC50 = 11 nM).

5 mg 481417

NF-κB SN50, Cell-Permeable Inhibitor Peptide (AAVALLPAVLLALLAPVQRKRQKLMP)

Contains the nuclear localization sequence (NLS) of the transcription factor NF-κB p50 linked to the hydrophobic region (h-region) of the signal peptide of Kaposi �broblast growth factor (K-FGF). The peptide N-terminal K-FGF h-region confers cell-permeability, while the NLS (360-369) inhibits translocation of the NF-κB into the nucleus.

500 µg1 mg

481480

NF-κB SN50M, Cell-Permeable Inactive Control Peptide (AAVALLPAVLLALLAPVQRNGQKLMP)

An inactive control for SN50 peptide (Catalogue No. 481480). Corresponds to the SN50 peptide sequence with substitutions of Lys363 for Asn and Arg364 for Gly in the NLS region.

500 µg 481486

Oridonin, R. rubescens A cell-permeable inhibitor of NF-κB activity that displays anti-proliferative (ED50 ~ 2.7 µg/mL in lymphoid malignant cells) and anti-angiogenic properties. Inhibits p65 NF-κB transcriptional activity by disrupting NF-κB DNA-binding activity without interfering with its nuclear translocation

5 mg 496915

(5Z)-7-Oxozeaenol, Curvularia sp. A cell-permeable fungal resorcylic lactone that acts as a selective, highly potent, ATP-comeptitive, irreversible inhibitor of MAPKKK TAK1 activity (IC50 = 8 nM).

1 mg 499610

Parthenolide, Tanacetum parthenium A sesquiterpene lactone with anti-in�ammatory, antisecretory, and spasmolytic properties that also inhibits NF-κB and activation of MAP kinase.

50 mg 512732

Pristimerin A cell-permeable dienone-phenolic triterpenoid that blocks proteasome chymotrypsin subunit (IC50 = 2.2 and 3.0 µM against puri�ed rabbit 20S and human 26S proteasome, respectively) and cellular NF-κB pathway/IKK activation.

5 mg 530070

Ro106-9920 A cell-permeable tetrazolopyridazine-phenylsulfoxide compound that acts as a highly selective, irreversible inhibitor of IκBαee ubiquitination (IC50 = 2.3 µM). Also reported to prevent degradation of IκBαee in MM6 cells (complete inhibition at ~3-5 µM).

1 mg5 mg

557550

TIRAP Inhibitor Peptide, Cell-Permeable (Ant-Tirap138-151)

A synthetic, cell-permeable peptide that corresponds to mouse toll-interleukin 1 receptor (TIR) domain-containing adapter protein 138-151 (TIRAP) fused to the Drosophila antennapedia sequence. Shown to speci�cally inhibit LPS-induced NF-κB activation. Also reported to block IκBα degradation.

1 mg 613570

TIRAP Inhibitor Peptide, Control, Cell-Permeable (Ant-Tirap151-138)

A cell-permeable synthetic peptide containing mouse toll-interleukin 1 receptor (TIR) domain-containing adapter protein 151-138 reverse sequence (TIRAP; also called Mal (MyD88-adapter-like), fused to the Drosophila antennapedia sequence. Serves as a control for TIRAP Inhibitor Peptide (Catalogue No. 613570).

1 mg 613571

(5Z)-Zeaenol, Curvularia sp. A cell-permeable resorcylic acid lactone compound that may serve as a negative control for TAK1 (TGF-β-Activated Kinase-1) inhibition studies.

1 mg 499609

Description Comments Qty/Pk Catalogue No.

InSolution™ NF-κB Activation Inhibitor A 10 mM (1 mg/281 µL) solution of NF-κB Activation Inhibitor (Catalogue No. 481406) in DMSO.

1 mg 481407

NF-κB Activation Inhibitor II, JSH-23 (4-Methyl-N1-(3-phenylpropyl)benzene-1,2-diamine)

A cell-permeable, selective blocker of the nuclear translocation of NF-κB p65 that does not affect IκB degradation (IC50 = 7.1 µM in LPS-stimulated macrophages RAW 264.7 stably transfected with NF-κB-SEAP-NPT). Shown to suppress DNA-binding of NF-κB.

5 mg 481408

NF-κB Activation Inhibitor III (3-Chloro-4-nitro-N-(5-nitro-2-thiazolyl)-benzamide, SM-7368)

A cell-permeable inhibitor of TNF-α-stimulated NF-κB activation and subsequent upregulation of MMP-9 in HT1080 cells (complete inhibition at 10 µM).

5 mg 481411

NF-κB Activation Inhibitor IV ((E)-2-Fluoro-4’-methoxystilbene)

A cell-permeable trans-stilbene Resveratrol (Catalogue No. 554325) analog that is shown to be ~130-fold more potent than Resveratrol in inhibiting TNF-α-stimulated NF-κB reporter activity in 293T cells (IC50 = 150 nM).

10 mg 481412

ANTI-PHOSPHO-AKT1 (Y326); 50 UG

ANTI-PHOSPHO-AKT (THR308), CLONE 50-1C-25; 100 UL

ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R) 488 CONJUGATE; 50 UG


ANTI-AKT3/PKBGAMMA, CLONE GMA104; 100 UG

ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1; 100 UG

ANTI-AKT1/PKBALPHA, CLONE AW24; 100 UL

ANTI-AKT3/PKBGAMMA; 100 UL

ANTI-PHOSPHO-AKT (SER473), CLONE 6F5; 100 UG

ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UL

ANTI-PHOSPHO-AKT1/PKBALPHA (THR308), CLONE NL50; 100 UL

ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE 11E6; 100 UG

ANTI-AKT2/PKBBETA, CLONE AW114; 100 UL


ANTI-AKT2/PKBBETA; 200 UG

ANTI-PHOSPHO-AKT (THR34); 100 UL

ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UG



ANTI-PHOSPHO-AKT1 (TYR326); 100 UG


ANTI-PHOSPHO-AKT (TYR450); 100 UG

ANTI-PI3 KINASE, P110DELTA RABBIT MONOCLONAL; 100 UL

ANTI-PI3 KINASE ALPHA, CLONE 2B12.1; 100 UG

ANTI-PI3 KINASE P85 ANTIBODY, N-SH2 DOMAIN , CAT. NO. ABS233

ANTI-PI3 KINASE, P110ALPHA; 200 UG

ANTI-PI3 KINASE, P110GAMMA; 100 UL

ANTI-PI3 KINASE, P110ALPHA RABBIT MONOCLONAL; 100 UL

ANTI-PI3K TYPE 3, CLONE EPR5301, RABBIT MONOCLONAL


ANTI-PI3 KINASE, P85, N-SH2, CLONE UB93-3; 200 UL

ANTI-PI3 KINASE ANTIBODY, P85 , CAT. NO. ABS234

ANTI-PI3 KINASE, P85; 125 UL

ANTI-PI3 KINASE, P110DELTA, CLONE AW103; 200 UG

ANTI-PI3 KINASE, P110BETA, RABBIT MONOCLONAL; 100 UL


ANTI-PI3 KINASE, P110BETA;100 UL

ANTI-PI3 KINASE BETA, P110B, CLONE 8C3.2; 100 UG

ANTI-PI3 KINASE, P110GAMMA, CLONE 17D7.2; 100 UG

ANTI-PI3-KINASE TYPE 3, CLONE 17D1.1; 100 UG

ANTI-PI3 KINASE-C2-ALPHA, CLONE 9H4.2; 100 UG

ANTI-PI3 KINASE-C2BETA; 100 UG

ANTI-PI3K TYPE 3

ANTI-PHOSPHO-RAF-1 (SER338); 100 UG

ANTI-RAF-1, CLONE AM223; 100 UL

ANTI-PHOSPHO-RAF-1 (TYR340/TYR341); 100 UL

ANTI-RAF-1, RABBIT MONOCLONAL; 100 UL


ANTImTOR/FRAP (AB-2) mouse MAB (22C2)

ANTImTOR, CLONE F11; 100 UG

ANTImTOR, RABBIT MAB; 100 UL

ANTI-PHOSPHOmTOR (SER2448)

ANTI-PHOSPHOmTOR (SER2481); 100 UL

ANTI-PHOSPHOmTOR (THR2446); 100 UL

ANTImTOR, A.A.1223-1290; 100 UG

ANTImTOR, CLONE 21A12.2; 100 UG

ANTImTOR, CLONE 2ID8.2; 100 UG

ANTImTOR/FRAP, CLONE EPR390(N), RABBIT MONOCLONAL

ANTImTOR/FRAP, CLONE 22C2


ANTI-PHOSPHOmTOR (THR2164)

ANTI-P70 S6 KINASE; 200 UG

ANTI-PHOSPHO-P70 S6 KINASE (THR389)

ANTI-P70 S6 KINASE (S6K); 200 UG

ANTI-PHOSPHO-P70 S6 KINASE (THR421/SER424), RABBIT

MONOCLONAL; 100 UL


ANTI-P70 S6 KINASE, RABBIT MONOCLONAL; 100 UL

ANTI-PHOSPHO-P70S6K (THR470)

ANTI-P70 S6 KINASE, CLONE 20-10C-6; 100 UL

大鼠;小鼠;鸡;马;犬;牛

人;小鼠;兔;牛;鸡

人;小鼠;大鼠

人;小鼠;大鼠

人

人;小鼠;大鼠

人;小鼠;大鼠

人

人;小鼠;大鼠;脊椎动物

人;小鼠

人;小鼠

小鼠;大鼠;人;犬

人;小鼠;大鼠

人;大鼠

人;小鼠;大鼠

人

人;小鼠

人;小鼠;牛;鸡;大鼠

人;小鼠;大鼠

人;小鼠;大鼠;犬;非洲爪蟾

人;小鼠;大鼠;牛;非洲爪蟾;犬

小鼠;大鼠;人;牛;鸡;马

人

小鼠

人;大鼠

人;脊椎动物

人

人

人;小鼠;大鼠

人;小鼠;大鼠

人;小鼠;大鼠

人;大鼠

人;小鼠;猴子;大鼠

人

人

人;大鼠

人;猴子;犬;小鼠;大鼠

大鼠;人;小鼠

人;小鼠;大鼠

人;小鼠;大鼠

小鼠;大鼠;人

人;黑猩猩;恒河猴

人;小鼠

小鼠

人;小鼠;大鼠

人

人;小鼠;大鼠

小鼠

人

人;大鼠

人;小鼠;大鼠

人

人;大鼠

人;小鼠;大鼠

人;大鼠

人

人;小鼠

人

人

人;小鼠;大鼠;羊;斑马鱼

人

人;小鼠;大鼠

人;小鼠

人;小鼠;大鼠

人

人

人;小鼠;大鼠

人

人;小鼠;大鼠

WB

WB;IF;ICC;ELISA

FC;ICC;IF;WB

FC;ICC;IF;WB

IP;WB

FC;ICC;IP;WB

IP;WB

IP;WB

ELISA;WB;FC;IF;IHC

WB

WB

ELISA;WB

WB;IP

IP;WB

IP;WB

WB

WB

WB

WB;IP

WB

IHC(P)

WB;Enzyme Assay

IF;IP;WB

WB

WB;IP

IP

FC;IP;WB

IF;IP;WB

WB;IHC

FC;ICC;IF;IHC(P);IP;WB

WB;IP

WB;IP

IP;WB

WB

FC;IP;WB

WB;IP;IHC;ELISA

WB;IP;ICC;IHC;ELISA

WB;ICC

WB;ICC

WB;ICC

WB;ICC

WB;IHC

ICC

WB

IP;WB

WB

IF;IHC(P);IP;WB

WB

WB;IP

WB;IP

FC;ICC;IHC(P);IP;WB

WB

WB

WB

WB;IP;IHC(P)

WB;ICC

WB;ICC

WB

WB;IP

WB;Peptide Inhibition

Assay;Dot Blot

Dot Blot;WB

IP;WB

WB;ICC

WB

IP;WB

WB;Peptide Inhibition Assay

FC;IHC(P);IP;WB

WB

WB;IP;ICC

50UG

100UL

50UG

50UG

100UG

100UG

100UL

100UL

100UG

100UL

100UL

100UG

100UL

100UL

200UG

100UL

100UG

100UL

100UL

100UG

100UL

100UG

100UL

100UG

100UG

200UG

100UL

100UL

100uL

100UL

200UL

200UL

125UL

200UG

100UL

100UG

100UL

100UG

100UG

100UG

100UG

100UG

100ug

100UG

100UL

100UL

100UL

200UG

100ug

100UG

100UL

100UG

100UL

100UL

100UG

100UG

100UG

100uL

100UG

100uL

100UL

200UG

100ug

200UG

100UL

100UG

100UL

200UL

100UL

09-288

05-802R

16-293

16-294

05-780

05-591

05-796

07-383

05-1003

04-736

05-802

05-669

04-771

05-771

07-372

07-789

05-736

04-802

04-796

AB9927

07-1398

07-1643

04-401

MABS76

ABS233

07-658

04-402

04-399

MABN1114

04-403

05-217

ABS234

06-195

05-703

04-400

09-481

09-482

05-1558

05-1559

05-1563

05-1560

07-2170

07-2186

05-534

04-739

07-815

04-412

05-538

OP97-100UG

MABS14

04-385

09-213

09-343

09-345

07-1415

05-1564

05-1592

MABS799

MABS196

ABS79

ABS88

07-402

MABS82

06-926

04-393

07-018-I

04-391

ABS431

05-781R

37

Oxidative Stress Pathway

Caspase-9 Caspase-3Apaf-1

ONOO-

NO•

NOS

SOD

GSHPeroxidase

GSHSynthase

GSHReductase

GSSG

GSH

Fe2+

Fe3+

Glycine

CysteineGlutamate

γ-glutamyl CysteineSynthase

NADP+

NADPH

L-arginine

CytC

Nuc

leus

Cell

Mem

bran

e

Apoptosis

ProteinOxidation

CellularDysfunction

Nitrosative StressInflammation

DNA Damage

CellDeath

LipidPeroxidation

(MDA)

ATP

∆ΨmMitochondrialDamage

CarbonylFormation (PCC)

Fenton Reaction

Telomere Attrition

Fe2+

Fe3+

OH•

O2

H+

H+

O2

O2

H2O

+

O2

H2O+

OH•

H2O2

H2O2

O2•-

O2•-O2

•-

O2•-

O2•-

I xelpmoC

III xel pmoC

II xel pmoC

VI xel pmoC

PTAesaht nyS

Inner Mitochondrial Membrane

H+

H+ H+H+H+

O2

H+

H+

ROS

ADP+Pi

ATP

氧化应激是指体内氧化与抗氧化作用失衡，倾向于氧化，导致中性粒细胞炎性浸润，蛋白酶分泌增加，产生大量氧化中间产

物。氧化应激是由自由基在体内产生的一种负面作用，并被认为是导致衰老和疾病的一个重要因素。自由基的一个主要功能是

激活宿主的免疫反应。炎症细胞产生的自由基和强氧化物对于杀死入侵的微生物具有重要的作用。然而，自由基的过量产生也

会引发细胞的致病过程。氧化应激水平升高诱发的最常见疾病有心脏病、癌症、骨关节炎、风湿性关节炎、糖尿病以及神经退

化性问题如阿尔兹海默症、帕金森病等。因此，了解自由基引发的氧化应激通路是十分重要的。

AB5605AB5830MAB3560

AB9890

07-520

MAB5310AB3282


MABN457

06-984

ABC391AB5380AB5382

AB16301AB553205-233

16-226

16-207

16-310

07-61007-35707-56107-562AB5484MAB5328

ABC245MABC684

AB10346AB5482AB9328











Western Blotting





Immunoprecipitation



Human



Mouse;Rat



HumanAllVertebrates

Vertebrates

All

All




200 µL200 µL100 µL

200 µL

200 µg

100 µg500 µg

100 µL100 µg100 µg100 µL

100 µg

200 µg

150 µL50 µL50 µL

100 µg100 µL100 µg

100 µg

50 µL

200 µL

50 µL100 µL200 µL200 µL200 µL100 µg

100 µg100 µL

50 µL50 µL100 µL


抗体

38

试剂盒

Milliplex kit

Small Molecules7-Nitroindazole 483400AAPH 100100AG126 658452α -Lipoic Acid 437692Apocynin 178385Carboxy-PTIO, Sodium Salt 217386Diphenyleneiodonium Chloride 300260DL-α -Difluoromethylornithine, Hydrochloride 288500DPPH Free Radical 300267FeTPPS 341492Keap1-Nrf2 Interaction Probe, ML334 505987L-NIL, Dihydrochloride 482100MCI-186 443300Methylene Blue 467250MnTMPyP 475872NG,NG-Dimethyl-L-arginine, Dihydrochloride 311203NG-Hydroxy-L-arginine, Monoacetate Salt 399250Nitric Oxide Synthase, Neuronal Inhibitor I 490070nNOS - PSD-95 Interaction Inhibitor, ZL006 482740NOX Inhibitor VIII, VAS3947 532336NOX1 Inhibitor, ML171 492002Nrf2 Activator 492040NS 2028 492030SKF-525A, Hydrochloride 567300

Description Cat. No.Description Cat. No.

OxyBlot™ Protein Oxidation Detection Kit S7150OxyELISA™ Oxidized Protein Quantitation Kit S7250OxyICC™ Oxidized Protein Detection Kit S7350OxyIHC™ Oxidized Protein Detection Kit S7450

AB5605AB5830MAB3560

AB9890

07-520

MAB5310AB3282


MABN457

06-984

ABC391AB5380AB5382

AB16301AB553205-233

16-226

16-207

16-310

07-61007-35707-56107-562AB5484MAB5328

ABC245MABC684

AB10346AB5482AB9328











Western Blotting





Immunoprecipitation



Human



Mouse;Rat



HumanAllVertebrates

Vertebrates

All

All




200 µL200 µL100 µL

200 µL

200 µg

100 µg500 µg

100 µL100 µg100 µg100 µL

100 µg

200 µg

150 µL50 µL50 µL

100 µg100 µL100 µg

100 µg

50 µL

200 µL

50 µL100 µL200 µL200 µL200 µL100 µg

100 µg100 µL

50 µL50 µL100 µL


抑制剂

Human Oxidative Stress(Cat.No.HØXSTMAG-18K)CatalasePeroxiredoxin 2(PRX2/PRDX2)Superoxide dismutase 1(SOD1)Superoxide dismutase 2(SOD2)Thioredoxin(TRX1)

Human Oxidative Phosphorylation(Cat.No.HØXSMAG-16K)Complex Ⅰ(NADH-Ubiquione Oxidoreductase)Complex Ⅱ(Succinate Ubiquione Oxidoreductase)Complex Ⅲ(Ubiquione Cytochrome C Oxidoreductase)Complex Ⅳ(Cytochrome C Oxidase)Complex Ⅴ(ATP Synthase)NNT(Nicotinamide Nucleotide Transhydrogenase

Rat/Mouse Oxidative Phosphorylation(Cat.No.RMHØXSMAG-17K)Complex Ⅰ(NADH-Ubiquione Oxidoreductase)Complex Ⅲ(Ubiquione Cytochrome C Oxidoreductase)Complex Ⅴ(ATP Synthase)

39

P53 SignalingP53是一个肿瘤抑制蛋白，调节各种各样基因的表达，包括细胞凋亡，生长抑制，抑制细胞周期进程，分化和加速DNA修复，基因毒性和细胞应激后的衰老等。在所有肿瘤中p53是最频繁突变的蛋白，据估计在60%的肿瘤中有变异形式，影响其生长抑制活动。一些常见的肿瘤有较高的发病率，90%的子宫癌和 70%的大肠癌中发现p53突变。

p53基因是研究最透彻，功能最强大的一种抑癌基因。野生型p53对细胞周期和凋亡起关键性作用，尤其是对受照射、细胞毒制剂、热疗打击的癌细胞，起更大的杀伤作用。其主要作用为：抑制并杀灭肿瘤细胞；与放、化疗手段协同，增强其杀灭癌细胞的功效，达到1+1大于2的效果；抑制肿瘤血管生成，有效防止肿瘤的复发、转移等。

由于P53可以阻滞肿瘤细胞G1/G0期，使肿瘤细胞不能进入S期，从而抑制肿瘤细胞增生。近年来，人们不断探索应用P53基因对肿瘤进行治疗，主要应用重组腺病毒-P53（rAd-P53）进行肿瘤临床研究。目前食管癌、卵巢癌、膀胱癌、非小细胞肺癌、乳腺癌等临床试验已取得阶段性成果，让人类在抗击肿瘤的斗争中看到了一丝曙光。

InhibitorSelect™ p53 PathwayRegulators Panel (Catalogue No. 506169)


ATM Kinase Inhibitor 2 mg 118500

ATM/ATR Kinase Inhibitor 5 mg 118501

Anacardic Acid 10 mg 172050

Cdk1 Inhibitor IV, RO-3306 5 mg 217699

Casein Kinase II Inhibitor III, TBCA

5 mg 218710

Chk2 Inhibitor II 1 mg 220486

Hdm2 E3 Ligase Inhibitor 5 mg 373225

JNK Inhibitor II 5 mg 420119

MDM2 Antagonist, Nutlin-3, Racemic

5 mg 444143

MDMX Inhibitor, SJ-172550 10 mg 444155

MG-132 5 mg 474790

p21-Activated Kinase Inhibitor III, IPA-3

5 mg 506106

Pi�thrin-α 1 mg 506132

PI-103 5 mg 528100

PARP Inhibitor VIII, PJ34 1 mg 528150

PPM1D Phosphatase Inhibitor 10 mg 529578

PRIMA-1 10 mg 530050

Caspase Inhibitor I 1 mg 627610

Trichostatin A, Streptomyces sp. 1 mg 647925

Anhydrous DMSO 15 mL KP31817

The Inhibitor panel includes:

Growth FactorsIonizingRadiationChemotherapyUVHypoxia

RTKs

JNKHIPK2CSNK1δ

p38

CKII

Chk1

ATR

DyrkCKI

CDK1

ATM

PAK4

AKT

S6K1

AMPK

mTOR

mTORC2

mTORC1

Chk2

DNA-PK

p53 Degradation

MDM2 Degradation

Apoptosis

Translation

Cell CycleProgression

DNA Damage

Premature Senescence

p53

p53

c-Abl

Caspase 6,7

PARP

HDAC

HAT

Proteasome

MDM2

p21CIP1

MDM2

CDC42

TSC2

MDMX

Cyclin D1

CyclinB

CDK2

CyclinE

Rb

Rb

WIP1TSC1

RHEB

GbLRictor

mTORGbLRictor

PI 3-K

E2F

CDK4

E2F

p53

p53Mutant

• p21-Activated Kinase Inhibitor III, IPA-3

• PARP Inhibitor VIII, PJ34

• Chk2 Inhibitor II

• ATM Kinase Inhibitor• ATM/ATR Kinase Inhibitor

• MG-132

• Casein Kinase II Inhibitor III, TBCA

• Anacardic Acid

• ATM/ATR Kinase Inhibitor

• JNK Inhibitor II, SP600125

• JNK Inhibitor II, SP600125

• JNK Inhibitor II,SP600125

• PPM1D Phosphatase Inhibitor

• Caspase Inhibitor I

• Cdk1 Inhibitor IV, RO-3306

• PRIMA-1

• MDMXInhibitor,

SJ-172550

• Trichostatin A, Streptomyces sp.

• Hdm2 E3 Ligase Inhibitor

• MDM2 Antagonist, Nutlin-3, Racemic

• MDM2 Antagonist,Nutlin-3, Racemic

• Pi�thrin-α

• JNK II,SP600125

• PI-103

• PI-103

• PI-103

Ac

Phosphorylation

Ubiquitin

Calbiochem® Inhibitors*以上组分可按目录号单独购买

40

Description Species Applications Catalogue No.

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Human, monkey, mouse, rabbit, rat OP03

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Human, monkey, mouse, rabbit, rat OP03L

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Human, monkey, mouse, rabbit, rat FC, FS, IF OP03F

Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) Human OP09

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Feline, human OP43

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Feline, human OP43L

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate Feline, human OP43A

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Fluorescein Conjugate Feline, human OP43F

Anti-p53 (Ab-7) (Pantropic) Sheep pAb Human, mouse, rat PC35

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) Human, mouse IB, IP OP104L

Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) Bovine, human, monkey OP140

Anti-p53 (pantropic), clone DO-1 Human IB, ICC, IHC, IF, IP MABE327

Anti-p53 R2 Human, mouse, rat IB AB4052

Anti-p53, Clone Pab421 Mouse IB, IP, IH MABE283

Anti-p53 Antibody, clone E26 Human, rat IB, IC, IH 04-241

Anti-p53 Antibody, clone E26 Human, rat IB, IC, IH Ab9985

Anti-p53 Antibody, aa 211-220, clone240 Bovine, chicken, human, hamster, mouse, monkey, rat

IB, IH, IP CBL404

Anti-p53 Antibody, clone BP53-12 Human IB, IH, IP 05-224

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29L

Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) Mouse, rat OP32

Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) Mouse, rat OP32L

Anti-p53 (Wild type), Clone Pab1620 Human, mouse IB, IH MABE339

Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Bovine, human, mouse, primate, rat. OP33

Anti-acetyl-p53 (Lys320) Antibody Bovine, chimpanzee, human IB 06-1283

Anti-acetyl-p53 (Lys373) Antibody, clone EP356(2)AY Human IB, IC, IH(P) 04-1137

Anti-acetyl-p53 (Lys382) Antibody, clone EPR358(2), Rabbit Human IB, IC 04-1146

Anti-acetyl-p53 Antibody (Lys373, Lys382) Most vertebrates IB 06-758

Anti-acetyl-p53 (Lys373) Antibody Human IB, IP 06-916

PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb Human, mouse, rat PC386

PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb Human PC461

PhosphoDetect™ Anti-p53 (pSer20) Rabbit pAb Human. mouse DR1023

PhosphoDetect™ Anti-p53 (pSer392) (Ab-4) Rabbit pAb Human, mouse IB PC387

PhosphoDetect™ Anti-p53 (pSer392) Mouse mAb (9F4) Human, mouse ELISA, IB 506133

PhosphoDetect™ Anti-p53 (pSer46) Rabbit pAb Human DR1024

Anti-phospho-p53 (Ser33) Antibody, clone EP2393Y Human IB, IC MABE199

Anti-phospho-p53 (Ser6) Antibody, clone Y179 Human IB, IC, IH, IP 04-540

Anti-phospho-p53 (Ser392) Antibody, clone EP155Y Human, rat IB, IC, IH(P), IC, IP 04-244

Anti-p53 (C-terminus) Antibody, clone E47 Human FC, IB, ICC, IP 04-242

Anti-p53 (N-term) Antibody, clone Y5 Human, rat IB, IC, IH(P), IP 04-1083

Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb Human, mouse PC712

Anti-p53 Binding Protein 1 Mouse mAb (BP13) Human DR1003

Anti-TP53BP2 Mouse mAb (3F8) Human ELISA, IB, IH (P) AP1163

*ELISA: enzyme linked immunosorbent assay; FC: �ow cytometry; FFS: free �oating sections; FS: frozen sections; GS: gel shift; IB: immunoblotting, IC: immunocytochemistry; IF: immuno�uorescence; IH: immunohistochemistry IP: immunoprecipitation; PS: paraf�n sections

P53抗体


Anti-p53 Antibody, aa 211-220, clone240 Bovine, chicken, human, hamster, mouse, monkey, rat

IB, IH, IP CBL404

Anti-p53 Antibody, clone BP53-12 Human IB, IH, IP 05-224

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29L

Anti-p53 (Wild type), Clone Pab1620 Human, mouse IB, IH MABE339

Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Bovine, human, mouse, primate, rat. OP33

Anti-acetyl-p53 (Lys320) Antibody Bovine, chimpanzee, human IB 06-1283

Anti-acetyl-p53 Antibody (Lys373, Lys382) Most vertebrates IB 06-758

Anti-acetyl-p53 (Lys373) Antibody Human IB, IP 06-916

PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb Human, mouse, rat PC386

PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb Human PC461

Anti-phospho-p53 (Ser6) Antibody, clone Y179 Human IB, IC, IH, IP 04-540

Anti-phospho-p53 (Ser392) Antibody, clone EP155Y Human, rat IB, IC, IH(P), IC, IP 04-244

Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb Human, mouse PC712

*ELISA: enzyme linked immunosorbent assay; FC: �ow cytometry; FFS: free �oating sections; FS: frozen sections; GS: gel shift; IB: immunoblotting, IC: immunocytochemistry; IF: immuno�uorescence; IH: immunohistochemistry IP: immunoprecipitation; PS: paraf�n sections

P53抗体参考文献:

The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain. Srinivasan, A, et al. Mol Cell Biol, 17:4761-73 (1997) 1997

Linear antigenic sites de�ned by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein. Legros, Y, et al. Oncogene, 9: 2071-6 (1994) 1994

Increased expression of mutant forms of p53 oncogene in primary lung cancer. Iggo, R, et al. Lancet, 335: 675-9 (1990) 1990

参考文献:

Tibbetts, R.S., et al. 1999. Genes Dev. 13, 152. Levine, A.J. 1997. Cell 88, 323. Shieh, S.Y., et al. 1997. Cell 91, 325.

参考文献:

Regulation of p53 in response to DNA damage. Lakin, N D and Jackson, S P, Oncogene, 18: 7644-55 (1999) 1999

p53, the cellular gatekeeper for growth and division. Levine, A J, Cell, 88: 323-31 (1997) 1997

p53: at the crossroads of molecular carcinogenesis and risk assessment. Harris, C C, Science, 262: 1980-1 (1993) 1993 82

41

Anti-NIK, C-terminus Human IB 100 µg AB16528

Anti-RelB Human IB, IC, IF 200 µL 07-1332

Anti-RelB Human, Mouse, Rat, Rhesus Monkey

IB, IH(P) 100 µg 06-1105

Anti-c-Rel (NF-κB) Human ELISA, EMSA, IB 100 µg 09-040

Anti-TBK1, clone AOW9 Canine, Chimpanzee, Human, Mouse

IB, IP 100 µL 04-856

Anti-TBK1/NAK (N-term), clone EP611Y Human, Mouse, Rat ICC, IB, IH(P),IP 100 µL 04-387

Anti-TBK1 Mouse (108A429) Human IB 100 µg AM76

Anti-Phospho-TBK1 (Ser172) Human, Mouse, Rat IB 100 µL 07-2192

Applications:ChIP: Chromatin

ImmunoprecipitationDB: Dot BlottingEMSA: Electrophoretic

Mobility Supershift Assay ELISA: Enzyme-linked

Immunosorbent Assay

FC: Flow Cytometry

IB: Immunoblotting

IC: Immunocytochemistry

IH: ImmunohistochemistryIP: ImmunoprecipitationPIA: Peptide Inhibition Assay




Anti-MDM2 (Ab-1) Mouse mAb (IF2) Human OP46

Anti-MDM2 (Ab-2) Mouse mAb (2A10) Human OP115

Anti-MDM2 (Ab-3) Mouse (4B11) Antibody Human , mouse IB, IF, IH (P), IP OP143

Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) Human IB, IF, IH (P), IP OP145

Anti-MDM2 (Ab-6) Mouse mAb (5B10C) Human IB, IF, IH(P), IP OP146

Human IB, IP, IC, IH, FC 04-1555

Anti-MDM2 Antibody, clone 3G9 Human, mouse IB, IP, IC, IH 04-1530

Anti-MDM4 Antibody, clone 7A8 Human, mouse IB 04-1556

Anti-MDM2, Clone IF2 Human IB, IH MABE340

Anti-MDM2, Clone 2A10 Human IB, IP MABE281

Anti-MDM2, Clone 4B2C1.11 Human. mouse IB, IC, IP MABE331

MDM2抗体

Anti-MDMx Antibody, clone 8C6

P53信号通路激活剂Description Comments Qty/Pk Catalogue No.

p53 Activator, Cell-Permeable A synthetic cell-permeable peptide corresponding to the C-terminal amino acids 361 - 382 of p53. Binds mutant p53 and restores the growth suppressor functions of p53 protein in human tumor cells.

500 µg 506131

p53 Activator II, Cell-Permeable A cell-permeable, proteolytically stable p53-activating peptide that displays antitumor properties.

500 µg 506144

p53 Activator III, RITA A cell-permeable, p53-targeting, tricyclic thiophene derivative that blocks p53-MDM2 interaction and p53 ubiquitination and induces p53-dependent apoptosis in tumor cells expressing wild-type p53.

1 mg 506149

PRIMA-1 A cell-permeable quinuclidinone analog that restores biochemical and biological function to mutant p53 and induces p53-dependent apoptosis in vitro and in vivo.

10 mg 530050

p53 Activator VII, STIMA-1 A cell-permeable compound that covalently modi�es free thiols on mutant p53 in H1299-His175 cells and fully restore its DNA binding activity to the level seen with wild-type p53, resulting in upregulation of p53-dependent gene expression.

25 mg 506168

p53 Modulator, CP-31398 A cell-permeable compound that protects wild-type p53 against heat-induced denaturation and locks newly synthesized mutant p53 in an active conformation. Unlike PRIMA-1, it directly targets the DNA binding domain of p53.

5 mg 506166

p53-Snail binding Inhibitor, GN25 A cell-permeable, speci�c p53-Snail binding inhibitor that activates p53 in a K-Ras-dependent manner and displays anti-proliferating effect toward K-Ras-transformed mouse embryonic �broblasts. Selectively activates wild-type p53 in p53WT/MT cancer cells.

25 mg 506170

Mutant p53 Reactivator, RETRA A cell-permeable compound that upregulates p53 family-responsive element-dependent reporter plasmid transcription activity in cells bearing Glu266, His273, Lys280, or Trp278 mutant p53 by increasing the cellular protein, but not mRNA, level of the p53 homologue p73 and by disrupting p73 interaction with mutant p53.

5 mg 506164

Tenovin-1 A cell-permeable compound that up-regulates cellular p53 protein, but not mRNA, level in MCF-7 (≥6-fold in 6 h), presumably by blocking MDM2-mediated p53 degradation.

10 mg 580566

RNA Ploymerase I Inhibitor II,CX-5461

5.09265.0001Inhibits RNA ploymerase I-mediated pre-rRNA transcription, but not Pol II-mediated c-myc transcription, by preventingTAF SL1 and rDNA association.

默克密理博近期发布了RNA

Polymerase I inhibitor II，

CX-5461，（cat.No.505487），

一种可渗透细胞的，高效的，

选择性和可逆的RNA聚合酶I介

导的核糖体RNA合成抑制剂，它

通过抑制合称起始阶段，但并

不影响RNA聚合酶II的活性。这

一化合物用于发表在Cancer Cell

杂志上一项十分重要的研究，

显示它在各种恶性细胞中诱导

p53依赖的细胞凋亡（在Em-Myc

淋巴瘤细胞中，IC50=5.4nM），

但是无论在野生型或非肿瘤耐

受的小鼠中，对于正常的B细胞

和脾细胞都没有影响。

42

(IC50 < 10 µM). Inhibits NF-κB -mediated gene transcription in cells by blocking the phosphorylation and degradation of IκBα.

IKK Inhibitor III, BMS-345541 (4-(2’-Aminoethyl)amino-1,8-dimethylimidazo[1,2-a]

quinoxaline)

A cell-permeable, potent, selective, and allosteric site-binding inhibitor of IKK-2 (IC50 ~300 nM) that displays anti-in�ammatory properties. Exhibits ~10-fold greater selectivity for IKK-2 over IKK-1 (IC50 ~4.0 µM).

1 mg 401480

IKK Inhibitor VII A cell-permeable benzamido-pyrimidine compound that acts as a potent, selective, and ATP-competitive inhibitor of IKK (IC50 = 40 nM, 70 nM, and 200 nM for IKK-2, IKK complex, and IKK-1, respectively).

1 mg 401486

IKK Inhibitor X (N-(6-Chloro-9H-β-carbolin-8-yl)nicotinamide)

A cell-permeable β-carboline that acts as a potent, ATP-competitive, and reversible inhibitor of IKK (IC50 = 88 nM) with selectivity over 14 other commonly studied kinases (IC50 > 100 µM), including NF-κB inducing kinase.

5 mg 401489

IKK Inhibitor XII A cell-permeable amino-diarylbenzamide compound that acts as a potent ATP site-targeting inhibitor against IKK-1 and IKK-2 (pIC50 = 6.4 and 7.0, respectively).

5 mg 401491

IKK Inhibitor XIII, 17-AJB A cell-permeable, irreversible inhibitor against IKK-β activity (IC50 = 300 nM). 1 mg 401493

IKK-2 Inhibitor IV ([5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide,

TPCA-1)

A cell-permeable, reversible, ATP-competitive, and potent inhibitor of IKK-2 (IC50 = 18 nM) that exhibits selectivity over IKK-1, JNK, and p38 MAPK.

500 µg 401481

InSolution™ IKK-2 Inhibitor IV A 10 mM (500 µg/179 µL) solution of IKK-2 Inhibitor IV (Catalogue No. 401481) in DMSO.

500 µg 401484

PI3-Kinase/Akt Signaling磷脂酰肌醇3-激酶(PI3Ks)蛋白家族参与细胞增殖、分化、凋亡和葡萄糖转运等多种细胞功能的调节。在

PI3K家族中，研究最广泛的是能被细胞表面受体所激活的I型PI3K。PI3K激活的结果是在质膜上产生第二

信使PIP3, PIP3与细胞内含有PH结构域的信号蛋白Akt和PDK1(phosphoinositide-ependent kinase-1)结合, 促使

PDK1磷酸化Akt蛋白的Ser308导致Akt的活化。Akt还能通过PDK2(如整合素连接激酶ILK)对其Thr473的磷酸化

而被激活。活化的Akt通过磷酸化作用激活或抑制其下游靶蛋白Bad、Caspase9、NF-κB、GSK-3、FKHR、p21Cip1和p27 Kip1等，进而调节细胞的增殖、分化、凋亡以及迁移等。

近年来发现，IA型PI3K和其下游分子蛋白激酶B(PKB或Akt)所组成的信号通路与人类肿瘤的发生发展密切

相关。该通路调节肿瘤细胞的增殖和存活，其活性异常不仅能导致细胞恶性转化，而且与肿瘤细胞的迁

移、黏附、肿瘤血管生成以及细胞外基质的降解等相关，目前以PI3K-Akt信号通路关键分子为靶点的肿

瘤治疗策略正在发展中。

Description Target Catalogue No.

Akt Inhibitor IV Akt 124011

Akt Inhibitor VIII, Isozyme-Selective, Akti-1/2 Akt1, Akt2 124018

LY 294002 PI3-K 440202

LY 303511 Negative control for LY 294002 440203

PDK1/Akt/Flt Dual Pathway Inhibitor Akt, Flt, PDK1 521275

PI-103 DNA-PK, PI3-K, mTOR 528100

PI3-K γ Inhibitor PI3-K γ 528106

PI3-K γ Inhibitor II PI3-K γ 528108

PI3-K α Inhibitor IV PI3-K α + β 528111

PI3-K α Inhibitor VIII DNA-PK, PI3-K 528116

Rapamycin mTOR, p70S6 553210

Ro-31-8220 PKC, GSK-3 557520

Wortmannin PI3-K Irreversible 681675

InhibitorSelect™ PI3-K/Akt/mTOR Signaling Pathway Inhibitor Panel (Catalogue No. 124031)

IGF-1

IGF-1R

PKCα

Growth Factors

RTKs

JAK1

PI 3-K

• LY 294002 • PI-103 • PI 3-Kγ Inhibitor • PI 3-Kγ Inhibitor II • PI 3-Kα Inhibitor IV • PI 3-Kα Inhibitor VIII • Wortmannin

• PDK1-Akt-Flt Dual Pathway Inhibitor • Akt Inhibitor IV, Akt Inhibitor VIII

• PDK1-Akt-Flt Dual Pathway Inhibitor

• PI-103, PI 3-Kα Inhibitor VIII

• Ro-31-8220

JAK1GAB1 BCAP

MDM2

MDM2Nucleus

p53

HSP90CDC37

Cytokines AG

Cytokine Receptor BCRBCR

4EBP1

elF4E

PDK-1

Raf1

• PI-103 • Rapamycin

mTor

p70s6K

PP2A

DNA-PK

SYK

Caspase 9

Caspase Cascade

ERK Pathway

Translation

Protein Synthesis

p53 Degradation

GlycogenSynthesis

GSK-3

Akt

Phosphorylation Ubiquitin GTP Calbiochem® Inhibitors

*以上组分可按目录号单独购买43



ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R)

488 CONJUGATE; 50 UG





ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, 100 UG







































ANTI-PI3K TYPE 3







人;小鼠;大鼠

人;小鼠;大鼠

人;小鼠;大鼠

人;小鼠;大鼠

人

人;小鼠;大鼠

人;小鼠;大鼠

人


人;小鼠

人;小鼠


人;小鼠;大鼠

人;大鼠

人;小鼠;大鼠

人

人;小鼠


人;小鼠;大鼠




人

小鼠

人;大鼠

人;脊椎动物

人

人

人;小鼠;大鼠

人;小鼠;大鼠

人;小鼠;大鼠

人;大鼠


人

人

人;大鼠


大鼠;人;小鼠

人;小鼠;大鼠

人;小鼠;大鼠

小鼠;大鼠;人


人;小鼠

小鼠

人;小鼠;大鼠

人

小鼠

WB

WB;IF;ICC;ELISA

FC;ICC;IF;WB

FC;ICC;IF;WB

FC;ICC;IF;WB

FC;ICC;IF;WB

IP;WB

FC;ICC;IP;WB

IP;WB

IP;WB

ELISA;WB;FC;IF;IHC

WB

WB

ELISA;WB

WB;IP

IP;WB

IP;WB

WB

WB

WB

WB;IP

WB

IHC(P)

WB;Enzyme Assay

IF;IP;WB

WB

WB;IP

IP

FC;IP;WB

IF;IP;WB

WB;IHC


WB;IP

WB;IP

IP;WB

WB

FC;IP;WB

WB;IP;IHC;ELISA

WB;IP;ICC;IHC;ELISA

WB;ICC

WB;ICC

WB;ICC

WB;ICC

WB;IHC

ICC

WB

IP;WB

WB

WB

50UG

100UL

50UG

50UG

50UG

100UG

100UG

100UG

100UL

100UL

100UG

100UL

100UL

100UG

100UL

100UL

200UG

100UL

100UG

100UL

100UL

100UG

100UL

100UG

100UL

100UG

100UG

200UG

100UL

100UL

100uL

100UL

200UL

200UL

125UL

200UG

100UL

100UG

100UL

100UG

100UG

100UG

100UG

100UG

100ug

100UG

100UL

100UL

200UG

09-288

05-802R

16-293

16-294

05-780

05-591

05-796

07-383

05-1003

04-736

05-802

05-669

04-771

05-771

07-372

07-789

05-736

04-802

04-796

AB9927

07-1398

07-1643

04-401

MABS76

ABS233

07-658

04-402

04-399

MABN1114

04-403

05-217

ABS234

06-195

05-703

04-400

09-481

09-482

05-1558

05-1559

05-1563

05-1560

07-2170

07-2186

05-534

04-739

07-815

04-412

05-538

MABS14

PI3-K/Akt 信号通路研究抗体产品描述反应种属应用体积货号

参考文献:

Phosphatidylinositol 3-kinase/AKT Signalling Pathway Components in Human Breast Cancer: Clinicopathological Correlations. Anticancer Res. 2007 Jul-Aug;27(4A):1777-82.

Tyrosine phosphorylation of Disabled-1 is essential for Reelin-stimulated activation of Akt and Src family kinases. doi:10.1016/S0169-328X(03)00295-X

Impaired PTPN13 phosphatase activity in spontaneous or HPV-induced squamous cell carcinomas potentiates oncogene signaling through the MAP kinase pathway. doi:10.1038/onc.2009.251

Catalase expression in MCF-7 breast cancer cells is mainly controlled by PI3K/Akt/mTor signaling pathway. doi:10.1016/j.bcp.2014.02.025

Ketamine Affects the Neurogenesis of Rat Fetal Neural Stem Progenitor Cells via the PI3K/Akt-p27 Signaling Pathway. DOI: 10.1002/bdrb.21119

44

PI3-K/Akt 信号通路研究抗体产品描述反应种属应用体积货号











































ANTI-PI3K TYPE 3




ANTI-RAF-1, RABBIT MONOCLONAL; 100 UL


ANTImTOR/FRAP (AB-2) mouse MAB (22C2)

ANTImTOR, CLONE F11; 100 UG

ANTImTOR, RABBIT MAB; 100 UL

ANTI-PHOSPHOmTOR (SER2448)


ANTI-PHOSPHOmTOR (THR2446); 100 UL

ANTImTOR, A.A.1223-1290; 100 UG

ANTImTOR, CLONE 21A12.2; 100 UG

ANTImTOR, CLONE 2ID8.2; 100 UG

ANTImTOR/FRAP, CLONE EPR390(N), RABBIT MONOCLONAL

ANTImTOR/FRAP, CLONE 22C2


ANTI-PHOSPHOmTOR (THR2164)

ANTI-P70 S6 KINASE; 200 UG


ANTI-P70 S6 KINASE (S6K); 200 UG

ANTI-PHOSPHO-P70 S6 KINASE (THR421/SER424), RABBIT

MONOCLONAL; 100 UL


ANTI-P70 S6 KINASE, RABBIT MONOCLONAL; 100 UL

ANTI-PHOSPHO-P70S6K (THR470)

ANTI-P70 S6 KINASE, CLONE 20-10C-6; 100 UL



人;小鼠;大鼠

人;小鼠;大鼠

人

人;小鼠;大鼠

人;小鼠;大鼠

人


人;小鼠

人;小鼠


人;小鼠;大鼠

人;大鼠

人;小鼠;大鼠

人

人;小鼠


人;小鼠;大鼠




人

小鼠

人;大鼠

人;脊椎动物

人

人

人;小鼠;大鼠

人;小鼠;大鼠

人;小鼠;大鼠

人;大鼠


人

人

人;大鼠


大鼠;人;小鼠

人;小鼠;大鼠

人;小鼠;大鼠

小鼠;大鼠;人


人;小鼠

小鼠

人;小鼠;大鼠

人

人;小鼠;大鼠

小鼠

人

人;大鼠

人;小鼠;大鼠

人

人;大鼠

人;小鼠;大鼠

人;大鼠

人

人;小鼠

人

人

人;小鼠;大鼠;羊;斑马鱼

人

人;小鼠;大鼠

人;小鼠

人;小鼠;大鼠

人

人

人;小鼠;大鼠

人

人;小鼠;大鼠

WB

WB;IF;ICC;ELISA

FC;ICC;IF;WB

FC;ICC;IF;WB

IP;WB

FC;ICC;IP;WB

IP;WB

IP;WB

ELISA;WB;FC;IF;IHC

WB

WB

ELISA;WB

WB;IP

IP;WB

IP;WB

WB

WB

WB

WB;IP

WB

IHC(P)

WB;Enzyme Assay

IF;IP;WB

WB

WB;IP

IP

FC;IP;WB

IF;IP;WB

WB;IHC


WB;IP

WB;IP

IP;WB

WB

FC;IP;WB

WB;IP;IHC;ELISA

WB;IP;ICC;IHC;ELISA

WB;ICC

WB;ICC

WB;ICC

WB;ICC

WB;IHC

ICC

WB

IP;WB

WB

IF;IHC(P);IP;WB

WB

WB;IP

WB;IP

FC;ICC;IHC(P);IP;WB

WB

WB

WB

WB;IP;IHC(P)

WB;ICC

WB;ICC

WB

WB;IP

WB;Peptide Inhibition

Assay;Dot Blot

Dot Blot;WB

IP;WB

WB;ICC

WB

IP;WB

WB;Peptide Inhibition Assay

FC;IHC(P);IP;WB

WB

WB;IP;ICC

50UG

100UL

50UG

50UG

100UG

100UG

100UL

100UL

100UG

100UL

100UL

100UG

100UL

100UL

200UG

100UL

100UG

100UL

100UL

100UG

100UL

100UG

100UL

100UG

100UG

200UG

100UL

100UL

100uL

100UL

200UL

200UL

125UL

200UG

100UL

100UG

100UL

100UG

100UG

100UG

100UG

100UG

100ug

100UG

100UL

100UL

100UL

200UG

100ug

100UG

100UL

100UG

100UL

100UL

100UG

100UG

100UG

100uL

100UG

100uL

100UL

200UG

100ug

200UG

100UL

100UG

100UL

200UL

100UL

09-288

05-802R

16-293

16-294

05-780

05-591

05-796

07-383

05-1003

04-736

05-802

05-669

04-771

05-771

07-372

07-789

05-736

04-802

04-796

AB9927

07-1398

07-1643

04-401

MABS76

ABS233

07-658

04-402

04-399

MABN1114

04-403

05-217

ABS234

06-195

05-703

04-400

09-481

09-482

05-1558

05-1559

05-1563

05-1560

07-2170

07-2186

05-534

04-739

07-815

04-412

05-538

OP97-100UG

MABS14

04-385

09-213

09-343

09-345

07-1415

05-1564

05-1592

MABS799

MABS196

ABS79

ABS88

07-402

MABS82

06-926

04-393

07-018-I

04-391

ABS431

05-781R

参考文献:

Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor. doi:10.1016/j.bbrc.2011.02.010

Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR. doi:10.1016/j.bcp.2012.01.019

Immunohistochemical assessment of PTEN in vulvar cancer: Best practices for tissue staining, evaluation, and clinical association. doi:10.1016/j.ymeth.2014.12.017

45

AB5605AB5830MAB3560

AB9890

07-520

MAB5310AB3282


MABN457

06-984

ABC391AB5380AB5382

AB16301AB553205-233

16-226

16-207

16-310

07-61007-35707-56107-562AB5484MAB5328

ABC245MABC684

AB10346AB5482AB9328











Western Blotting





Immunoprecipitation



Human



Mouse;Rat



HumanAllVertebrates

Vertebrates

All

All




200 µL200 µL100 µL

200 µL

200 µg

100 µg500 µg

100 µL100 µg100 µg100 µL

100 µg

200 µg

150 µL50 µL50 µL

100 µg100 µL100 µg

100 µg

50 µL

200 µL

50 µL100 µL200 µL200 µL200 µL100 µg

100 µg100 µL

50 µL50 µL100 µL

Akt 抑制剂Product Cat. No. Comments Size Price

Akt Inhibitor 124005 A PI analog, prevents PIP3 formation and binding to Akt. Inhibits Akt (IC50 = 5.0 M) and PI 3-K (IC50 = 83 M)

1 mg

Akt Inhibitor II (SH-5) 124008 A PI analog, prevents PIP3 formation and binding to Akt. Metabolically more stable than Akt Inhibitor (Cat. No. 124005).

1 mg

Akt Inhibitor III (SH-6) 124009 A PI analog, prevents PIP3 formation and binding to Akt. Metabolically more stable than Akt Inhibitor (Cat. No. 124005).

1 mg

Akt Inhibitor IV 124011 A cell-permeable, ATP-competitive inhibitor of a kinase upstream of Akt, but downstream of PI 3-K.

1 mg5 mg

InSolution™Akt Inhibitor IV

124015 A 10 mM solution of Akt Inhibitor IV (Cat. No. 124011) in DMSO. 1 mg

Akt Inhibitor V, Triciribine (API-2, NSC 154020, TCN)

124012 A cell-permeable inhibitor of Akt. Blocks cellular phosphorylation/activation of Akt1/2/3 by targeting an Akt effector molecule other than PI 3-K or PDK1. Has shown ef�cacy in vivo.

1 mg

Akt Inhibitor VI, Akt-in 124013 A15-mer peptide that directly binds Akt PH domain (kd=18 M), preventing PI binding. 2 mg

Akt Inhibitor VII, TAT-Akt-in 124014 A cell-permeable version of Cat. No. 124013 that directly binds Akt PH domain, preventing PI binding. Has shown ef�cacy in vivo.

2 mg

Akt Inhibitor VIII, Isozyme-Selective, Akt-1/2

124018 A cell-permeable inhibitor of Akt. Inhibition is PH domain dependent. Shown to block basal and stimulated phosphorylation/activation of Akt1/Akt2 in cultured cells in vitro and in mice in vivo (IC50 = 58 nM, 210 nM, and 2.12 M for Akt1, Akt2, and Akt3, respectively, in vitro kinase assays).

1 mg

InSolution™ Akt Inhibitor VIII, Isozyme-Selective, Akti-1/2

124017 A 10 mM (1 mg/181 µl) solution of Akt Inhibitor VIII, Isozyme-Selective, Akti-1/2 (Cat. No. 124018) in DMSO.

1 mg

Akt Inhibitor IX, API-59CJ-OMe

124019 A cell-permeable ellipticine compound that potently and selectively inhibits cell growth and induces apoptosis in human endometrial cancer cells with elevated Akt levels. Exhibits minimal effect on cells with low Akt activity.

5 mg

Akt Inhibitor X 124020 A cell-permeable, selective inhibitor of the phosphorylation of Akt and its in vitro kinase activity (complete inhibition < 5 M) with minimal effect on PI 3-K, PDK1, or SGK1. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent.

5 mg

Akt Inhibitor XI 124028 A cell-permeable copper complex (Cu2+/Cu+ redox couple in the range of +0.28 to +0.35 V) that interacts with both the PH and the kinase domains of Akt and potently inhibits its kinase activity (IC50 = 100 nM). Inhibits tumor growth both in cultured cells in vitro (IC50 ranges from 10 to 34 M) and in mice in vitro (25 mg/kg, iv) without any apparent adverse effect to the animals.

5 mg

(-)-Deguelin, Mundulea serica

252740 A cell-permeable, potent inhibitor of mitochondrial bioenergetics (IC50 = 6.9 nM for NADH: ubiquinone oxidoreductase activity in bovine heart ETP). Promotes mitochondrial permeability transition. Selectively blocks Akt activation with minimal effects on MAPK signaling. Also shown to activate AMPK activity and inhibit COX-2 expression.

5 mg

Naltrindole, Hydrochloride 476880 A cell-permeable inhibitor of Akt signaling. Decreases phosphorylation level of PDK1, Akt, FKHR/AFX, GSK-3 , and inhibits Akt-dependent cell growth in small cell lung cancer (SCLC) cell line (IC50 = 25, 40, and 55 M in NCI-H69, NCI-H345, and NCI-H510, respectively).

5 mg

PDK1/Akt/Flt Dual Pathway Inhibitor

521275 A cell-permeable inhibitor of PDK1 and Akt in in vitro kinase assays. Blocks phosphorylation of Akt at both Ser473 and Thr308.

5 mg

mTOR 抑制剂Product Cat. No. Comments Size Price

Rapamycin 553210 Anti-fungal and immunosuppressant. Inhibits mTOR by binding to FK506-binding protein-12. Selectively inhibits the phosphorylation and activation of p70 S6 kinase (IC50 = 50 pM). Prevents the translational activation of IGF-II. Shown to inhibit later signaling events such as p110Rb phosphorylation, p34cdk1 kinase activation, and cyclin A synthesis. Reported to induce apoptosis in a murine B cell line, to inhibit lympho-kine-induced cell proliferation at the G1 phase, and to irreversibly arrest Saccharomyces cerevisiae G1 phase.

100 g 1 mg

InSolution™ Rapamycin 553211 Supplied as a 5 mM (500 g/109 l) solution of Rapamycin (Cat. No. 553210) in DMSO. 500 g

PI-103 528100 A cell-permeable pyridinylfuranopyrimidine compound that acts as a potent and ATP-competitive inhibitor of DNA-PK, PI 3-K, and mTOR (IC50 = 2, 8, 88, 48, 150, 26, 20, and 83 nM for DNA-PK, p110 , p110 , p110 , p110 , PI 3-KC2 , mTORC1, and mTORC2, respectively). It inhibits ATR and ATM only at much higher concentrations (IC50 = 850 and 920 nM, respectively) and exhibits little activity towards a panel of more than 40 other kinases even at concentrations as high as 10 M. Shown to effectively block PI 3-K/Akt signaling and cell proliferation in glioma cell lines both in vitro and in vivo.

1 mg 5 mg

46

PI 3-Kinase 抑制剂Product Cat. No. Comments Size Price

ET-18-OCH3 341207 A selective cell-permeable inhibitor of phosphatidylinositol-speci�c PLC (IC50 = 15 mM) but does not inhibit phosphatidylcholine-speci�c PLC or PLD.

5 mg

DNA-PK Inhibitor III 260962 A cell-permeable, potent, selective, ATP-competitive inhibitor of DNA-PK (IC50 = 120 nM) and PI 3-Kinase catalytic subunit p110b (IC50 = 135 nM). Inhibits PI 3K p110a , p110g, and p110d only at much higher concentrations (IC50 = 1.4, 0.88, and 1.0 mM, respectively).

1 mg

LY 294002 440202 A cell-permeable, potent, and speci�c inhibitor of PI 3-K (IC50 = 1.4 mM). Acts on the ATP-binding site of the enzyme.

5 mg

InSolution™ LY 294002 440204 Supplied as a 10 mM (1 mg/325 ml) solution of LY 294002 (Cat. No. 440202) in DMSO. 1 mg

LY 303511 440203 A cell-permeable negative control for the PI 3-kinase inhibitor, LY 294002 (Cat. No. 440202). Contains a single atom substitution in the morpholine ring compared to LY 294002. Does not affect PI 3-kinase activity even at concentrations ≥100 mM.

1 mg

PI-103 528100 A cell-permeable pyridinylfuranopyrimidine compound that displays antitumor properties in a mouse model of human glioma. Acts as a potent and ATP-competitive inhibitor of DNA-PK, PI 3-K and mTOR (IC50 in nM = 2, 8, 88, 48, 150, 26, 20 and 83 for DNA-PK, p110a , p110b, p110d, p110g, PI 3-KC2b, mTORC1 and mTORC2, respectively) and exhibits little activity towards a panel of more than 40 other kinases, even at concentations as high as 10 mM. Reported to block PIP3 production, insulin signaling and Akt-phosphorylation, and to inhibit cell proliferation and induce cell-death.

1 mg 5 mg

PI 3-Kg Inhibitor 528106 A cell-permeable thiazolidinedione compound that acts as a potent, selective, and ATP-competitive inhibitor of PI 3-Kg (Ki = 7.8 nM; IC50 = 8 nM, 60 nM, 270 nM, 300 nM for p110-g, a , b and d-isoforms, respectively). Does not affect the activity of a wide panel of kinases, receptors, enzymes, and ion channels even at concentrations as high as 1 mM.

5 mg

PI 3-Kg Inhibitor II 528108 A cell-permeable, potent and ATP-competitive inhibitor of PI 3-Kg (Ki = 180 nM; IC50 = 250 nM). Exhibits great selectivity over PI 3-Ka (IC50 = 4.5 mM), PI 3-Kb and d (IC50 >20 mM).

5 mg

PI 3-Kg/CKII Inhibitor 528112 A cell-permeable, potent and ATP-competitive dual speci�c inhibitor of PI 3-Kg;/CKII (IC50 = 20 nM).

5 mg

Quercetin, Dihydrate 551600 An inhibitor of PI 3-kinase (IC50 = 3.8 mM) and phospholipase A2 (IC50 = 2 mM). 100 mg

Wortmannin 681675 A potent, selective, and irreversible inhibitor of PI 3-K (IC50 = 5 nM). Blocks the catalytic activity of PI 3-kinase without affecting the upstream signaling events.

1 mg

47

TGF-β Signaling转化生长因子-β(TGF-β)以及与其关系密切的骨形成蛋白(BMPs)参与调节分化、衰老、免疫反应、愈伤、凋亡等等重要生命过

程。TGF-β超家族包含接近30个生长和分化因子，其中有TGF-βs，activin，inhibins和BMPs。下游的跨膜TGF-β受体是多个SMAD

蛋白，这些蛋白是TGF-β超家族信号传递的重要调控分子，并在不同层面上受多种多样精确的调控。

MM 匹配抗体名MM匹配抗体编号 Key Applications Species Reactivity Qty/Pk

抗体

ABC345

MABC525

MAB4429

MAB4350

MABT117

05-1424

CBL1358

ABD7107-049MABE902AB4227

Anti-BAG-1 Antibody

Anti-BAG-1 Antibody, clone 3.10G3E2Anti-BMP-7 (Pro-peptide) Antibody, clone 2Anti-BMP-7 Antibody, clone 2A10Anti-Endoglin (CD105) Antibody, clone 44G4Anti-Endoglin Antibody, clone P3D1

Anti-Endoglin Antibody, Extracellular, clone MJ7/18Anti-FOXC1 AntibodyAnti-Nedd4 Antibody, WW2 domainAnti-Pax-2 Antibody, clone 4C2.2 Anti-Pax5 Antibody

Western Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;Immunoprecipitation;Immunohistochemistry;ELISAWestern Blotting;Immunohistochemistry (Paraffin);ELISA;ImmunocytochemistryWestern Blotting;ImmunohistochemistryFlow Cytometry;Immunohistochemistry;ImmunoprecipitationWestern Blotting;Immunohistochemistry;Immunocytochemistry;Immunoprecipitation;Flow Cytometry;ELISAFlow Cytometry;Immunoprecipitation;Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryImmunohistochemistry;Western BlottingWestern BlottingWestern Blotting

Human

Human

Human

Human;Mouse

Human

Human;Mouse;Rat

Mouse

Human;MouseHuman;Mouse;RatHumanHuman;Mouse

100 µg

100 µL

100 µg

100 µg

100 µL

100 µg

500 µg

100 µg200 µL200 µL200 µg

48


抗体

05-1573

MABN711MABE1041

ABS52

FCMAB108A4

05-857

05-1478

05-1459

04-953

AB3849

06-920MABT521

ABE21ABD104

ABC330ABD133ABN476MABF347

MABF345

MABS477

MABF348

MABF346

06-227

ABD73MABD14609-726

Anti-Pax5 Antibody, clone 1H9

Anti-PAX5 Antibody, clone 7D3Anti-Pax-9 Antibody, clone 7C2 (P9C23) Anti-phospho-SMAD1 (Ser206) AntibodyAnti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor® 488Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonalAnti-RUNX2 Antibody, clone AS110Anti-SMAD1 Antibody, clone AS22Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonalAnti-Smad2 Antibody, phospho-specific (Ser465/467)

Anti-Smad3 AntibodyAnti-SMAD3, clone 5G11 AntibodyAnti-Smad4/DPC4 AntibodyAnti-SnoN Antibody

Anti-ACVR1 AntibodyAnti-ACVR1B AntibodyAnti-ACVR1C AntibodyAnti-LAP (TGF-β1) (human) Antibody, FITC, clone TW4-2F8Anti-LAP (TGF-β1) (mouse) Antibody, APC, clone TW7-16B4Anti-LAP (TGF-β1) Antibody, clone TW4-6H10Anti-TGF-β1 (human) Antibody, PE, clone TW4-2F8Anti-TGF-β1 (mouse) Antibody, PE, clone TW7-16B4Anti-TGFβR Type II Antibody, NT

Anti-Lefty AntibodyAnti-Lefty Antibody, clone 1G9.1Anti-PAI-1 Antibody

Western Blotting;Immunohistochemistry;ImmunoprecipitationWestern BlottingWestern Blotting;Immunohistochemistry

Western Blotting

Western Blotting;Flow Cytometry

Western Blotting


Western Blotting;Immunofluorescence

Western Blotting

Immunocytochemistry;Immunofluorescence;Immunohistochemistry (Paraffin);Western BlottingWestern BlottingWestern Blotting;Immunocytochemistry

Western Blotting;ImmunocytochemistryWestern Blotting;Immunocytochemistry;ImmunohistochemistryImmunohistochemistryWestern BlottingWestern BlottingFlow Cytometry

Flow Cytometry;Western Blotting;Immunoprecipitation;NeutralizingFlow Cytometry

Flow Cytometry

Flow Cytometry;Western Blotting;Immunoprecipitation;NeutralizingImmunohistochemistry;Immunoprecipitation;Western BlottingWestern Blotting;ImmunocytochemistryWestern BlottingWestern Blotting

Mouse;Human

HumanMouse;Human

Human

Human;Xenopus;Bovine

Mouse;Vertebrates

Human

Human;Mouse

Canine;Human;Mouse;Zebrafish

Human;Mouse;Rat


Human;Mouse;Rat;ChimpanzeeHuman

HumanHumanHumanHuman

Mouse

Human

Human

Mouse

Human;Mouse;Rat

Mouse;RatRat;MouseHuman

100 µg

100 µL100 µg

100 µg

100 tests

100 µL

100 µg

200 µg

100 µL

100 µL

400 µg100 µL

100 µg100 µg

100 µg100 µg100 µg100 tests

100 µg

100 µg

100 tests

100 µg

400 µg

100 µg100 µg100 µg

49

InhibitorSelect™TGF-βR and BMPR Signaling Pathway Inhibitor Panel,目录号616466


Akt Inhibitor XIII,

Isozyme-Selective,Akti2-1/2

AMPK Inhibitor,Compound C

ET-18-OCH3

IRAK-1/4 Inhibitor

JAK Inhibitor Ⅰ

MG-132

(5Z)-7-Oxozeaenol,

Curvularia sp.

p21-Activated Kinase

Inhibitor Ⅲ,IPA-3

PD 169316

Akt1/2

BMPRI;AIk2/3/6

PI-PLC

IRAK-1& IRAK-4

JAK1/2/3,Tyk2

Proteasome

TAK1

PAK1/2/3

MAPK Smad

2mg

1mg

5mg

5mg

500μg

5mg

1mg

5mg

1mg

124030

171260

341207

407601

420099

474790

499610

506106

513030


PI-103

Rac1 Inhibitor

Ro-31-8220

Smad3 Inhibitor,SIS3

STAT3 Inhibitor Ⅲ,WP1066

TGF-β RI Kinase Inhibitor Ⅱ

TGF-β RI Kinase Inhibitor Ⅵ,

SB431542

U0126

Y-27632

DMSO-15ML

DNA-PK,PI3-K,mTOR

Rac1

PKC,GSK-3,SIRT1/2

Smad3

JAK-STAT3 Sig Inh

TGF-β RI;AIk5

TGF-β RI;AIk4/5/7

MEK1/2

Rho Kinase

-

1mg

5mg

500μg

1mg

10mg

1mg

5mg

1mg

1mg

15ml

528100

553502

557520

566405

573097

616452

616461

662005

688000

KP31817

TGFβ - 6 Plex(Cat.No.48-614MAG)AB2


Akt/PKB

Erk/MAPK1/2

SMAD2

SMAD3

SMAD4

TGFβRII

√

√

√

√

√√ (Ser473)

(Thr185/Tyr187)

(Ser465/467)

(Ser423/425)

H

H,M,R

H,M,R

H,M,R

H,M,R

H

Milliplex Kit


50

Toll-like Receptor (TLR) Signaling Toll-like Receptors (TLRs)是在多种免疫细胞中表达的跨膜蛋白。胞外的N末端区域识别特异的病原组分。目前已发现至少13个TLR家

族蛋白识别不同的病原物相关的分子结构（PAMPs），包括脂多糖，鞭毛蛋白，细菌CpG DNA，和病毒RNA、DNA。TLRs识别

PAMPs被认为是诱导免疫反应的关键步骤。TLRs也会和补体系统一起作用提供关键的先天防御。它们通过相互作用和拮抗反应来

加强先天免疫并调节过度的炎症反应。补体可以抑制TLR介导产生的IL-12。有报道显示巨噬细胞中C5aR的活化可以抑制TLR4诱导

的IL-12p35，IL-12/IL-23p40，IL-23p19 和IL-27p28 的mRNA表达，以及IL-12，IL-23 和IL-27蛋白的生成。其作用机制是PI-3 kinase和

ERK1/2信号通路诱导活化，抑制了调节IL-12家族蛋白表达的主要转录因子IRF-1和IRF-8。

TLRs也会和炎性体一起作用诱导IL-1和IL-8的分泌，这个过程主要包括两步：首先，TLR或NOD（nucleotide binding oligomerization

domain containing protein）信号通路诱导pro-IL-1和pro-IL-18转录。然后，通过炎性体的作用进行蛋白水解过程。第一步由TLR配

体或TNF-a诱导，第二步的信号由成孔毒素（pore-forming toxins），淀粉样肽（amyloid peptides），尿酸结晶（uric acid crystals）

和K+ ef�ux 发出。

DescriptionMM cat# Key Applications Species Reactivity Qty/Pk

抗体

05-591

05-1003

05-802R

06-74807-175

MAB3026

09-481

Akt/PKB Antibody, PH Domain, clone SKB1

phospho-Akt (Ser473) Antibody, clone 6F5

phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonalJNK/SAPK1 Antibodyphospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody

NFκB Antibody, p65 subunit, active subunit, clone 12H11

PI3 Kinase Antibody, p110α

Flow Cytometry;Immunocytochemistry;Immunoprecipitation;Western BlottingELISA;Western Blotting;Flow Cytometry;Immunofluorescence;ImmunohistochemistryWestern Blotting;Immunofluorescence;Immunocytochemistry;ELISAImmunoprecipitation;Western BlottingWestern Blotting;Enzyme Assay;Immunoprecipitation

Electrophoretic Mobility Shift Assay;Flow Cytometry;Immunocytochemistry;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Western BlottingWestern Blotting;ELISA;Immunoprecipitation;Immunohistochemistry

Human;Mouse;Rat

Human;Mouse;Rat;Vertebrates

Human;Mouse;Rabbit;Bovine;Chicken

Human;Mouse;RatMouse;Human;Rat;Canine;Xenopus;Chicken;Bovine;HorseHuman;Mouse;Rat;Rabbit

Human;Rat

100 µg

100 µg

100 µL

200 µg200 µg

100 µg

100 µg

Nucleus

Bacterial Antigens

Growth Factors

Growth FactorReceptors

TNFα

Proteosome

TNFR1 IL-1R

IL-1

NF-κBNF-κBNF-κB

NF-κB

p50c-Rel

NF-κB

IκBs

HDAC3

p50

p50

p50Bcl3

p50

p105p105

ABIN-2Tpl2

LPS

TRADDTRAF2

p300

CBP

TRAF6

TRAF6

MALPPGN

MyD88TOLLIPMyD88

PI 3-K

PDK-1

ASK1

MKK4,7

JNKsAkt/PKB

IKK-γ

MEKKs

Tpl2

MEKK

ERK

PKR PKR

IRAKIRAK

IRAK

TAK1

IKK-α IKK-β

JNK PathwayERK

Signaling

Tpl2Degradation

Virus

IκB Degradation

Nuclear Import

SCF/β-TrCP

RelBp52NF-κB

IκBs

IκBs

p50c-Rel

NF-κB

IκBs p52p65

NF-κB

κ-BRas

IκBs p50p65

NF-κB

IκB

IκBs p52p65

NF-κB

PACT

Nuclear Export

AcetylationDeacetylation

Phosphorylation

Ubiquitin


51

DescriptionMM cat# Key Applications Species Reactivity Qty/Pk

抗体

05-53505-536

05-63107-1007

07-1008

06-1110MABC988

MABF349

MABF350

09-040

05-813

07-1483

05-53605-53506-1089ABF303

06-1115

06-87206-41305-1469

AB16528ABS1515

06-1340

ABS160

07-2192

07-1332

MABF84

06-1110

Anti-IKKβ Antibody, clone 10AG2Anti-IKKα Antibody, clone 14A231Anti-IKKγ AntibodyAnti-IKKα Antibody

Anti-IKKβ Antibody

Anti-TRAF6 AntibodyAnti-TRADD Antibody, clone 1G6.1 Anti-CD282 (TLR2) (human) Antibody, PE, clone TL2.1Anti-CD284 (TLR4) (human) Antibody, PE, clone HTA125Anti-c-Rel (NFκB) Antibody

Anti-HDAC3 Antibody, clone 3G6

Anti-IκBα Antibody

Anti-IKKα Antibody, clone 14A231Anti-IKKβ Antibody, clone 10AG2Anti-IL-12R-beta-1 AntibodyAnti-IL18R1 Antibody

Anti-Interleukin-18 (IL-18) AntibodyAnti-IRAK AntibodyAnti-NFκB p52 AntibodyAnti-NFĸB Antibody, p65, clone 1G10.2Anti-NIK Antibody, CTAnti-phospho NFkB p52, (Ser222) AntibodyAnti-phospho-IKK-epsilon (Ser172) AntibodyAnti-phospho-MKK4 Antibody (Ser257/Thr261)Anti-phospho-TBK1 (Ser172) AntibodyAnti-RelB (NFĸB family member) AntibodyAnti-TLR2 Antibody, clone 19B6.2

Anti-TRAF6 Antibody


Western BlottingWestern Blotting;Immunoprecipitation

Western Blotting

Western Blotting;ImmunohistochemistryWestern Blotting;Immunohistochemistry (Paraffin)Flow Cytometry

Flow Cytometry;Immunohistochemistry;ImmunofluorescenceELISA;Electrophoretic Mobility Shift Assay;Western BlottingImmunoprecipitation;Western Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin);ELISA;ImmunoprecipitationWestern BlottingWestern BlottingWestern Blotting;ImmunohistochemistryWestern Blotting;Immunohistochemistry;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Immunoprecipitation;Western BlottingImmunoprecipitation;Western BlottingWestern Blotting;Immunofluorescence;ImmunocytochemistryWestern BlottingWestern Blotting

Western Blotting


Western Blotting

Western Blotting;Immunocytochemistry;ImmunofluorescenceWestern Blotting;Immunohistochemistry (Paraffin);Flow CytometryWestern Blotting;Immunohistochemistry

Human;Rat;MouseHuman;Mouse;Monkey

Human;MouseHuman;Canine;Horse;Bovine;Rat;MouseHuman;Canine;Chicken;Bovine;Feline;MouseHuman;Horse;Rhesus MacaqueHuman;Rat

Human

Human

Human

Bovine;Human;Hamster;MouseHuman;Mouse;Rat

Human;Mouse;MonkeyHuman;Rat;MouseHumanHuman

Human;Chimpanzee;Rhesus Macaque;PrimateHuman;MouseHumanHuman;Mouse;Rat;Bovine

HumanHuman

Human;Mouse;Rat

Human;Mouse;Rat;Bovine;Xenopus;ZebrafishHuman;Mouse;Rat

Human

Human

Human;Horse;Rhesus Macaque

100 µg100 µg

100 µg150 µg

150 µg

100 µg100 µg

100 tests

100 tests

100 µg

200 µg

100 µL

100 µg100 µg100 µg100 µL

100 µg

200 µg100 µL200 µg

100 µg100 µL

100 µL

100 µL

100 µL

200 µL

200 µg

100 µg

试剂盒

IκB STAR ELISA Kit 17-485MILLIPLEX® MAP NF?B Magnetic Bead Kit 6-plex 48-630MAGMILLIPLEX® MAP Phospho NF-κB (Ser536) Magnetic Bead MAPmate™ 46-702MAGMILLIPLEX® MAP Total NF-κB Magnetic Bead MAPmate™ 46-701MAG

抑制剂(5Z)-7-Oxozeaenol, Curvularia sp. 499610BAY 11-7082 196870Histone Acetyltransferase Inhibitor II 382110IKK Inhibitor VII 401486Interleukin 1- Receptor-Associated-Kinase-1/4 Inhibitor 407601MEK Inhibitor II 444938NEMO-Binding Domain-Binding Peptide, Cell-Permeable 480025NF-κB SN50, Cell-Permeable Inhibitor Peptide 481480TIRAP Inhibitor Peptide, Cell-Permeable 613570Tpl2 Kinase Inhibitor 616373Trichostatin A, Streptomyces sp. 647925

52

Wnt SignalingWnt信号通路在进化中相当保守，参与细胞生命法则、发育、细胞增殖、细胞极化及迁移等过程。Wnt基因编码庞大的蛋白家

族，这些分泌性蛋白富含半胱氨酸，甚至在成体组织中对于发育和维持稳定也起着关键作用。如果这条信号通路中的关键蛋白

发生突变，导致信号异常活化，就可能诱导癌症和退行性疾病的发生。因此，对于这条信号通路的研究，需要一套小分子化合

物用于精确、完全的干扰调控，而InhibitorSelect™ Wnt信号通路抑制剂套装（含有15种小分子化合物）正是为应对这种实验要求

而设计的。


抗体

MABD170MAB3786

05-1579

06-1050

MAB4350

ABE477MABS420

MABD122

MABE56907-1231

ABD13MABF108MABN709ABN40MABE69MAB3582

ABD71AB5687ABE1828ABE68

ABE73ABE7506-756ABE1351ABC217

07-1413

AB5702AB10050

06-1097MABC155MABD3507-1385MABD163

MABD102

MABS341AB5975AB5977

AB9220MABD24

AB5731MABC774

MAB5556

Anti-AFP Antibody, clone 6E6Anti-APC Antibody, CT, clone C-APC 28.9Anti-Axin-1 Antibody, clone A5

Anti-Axin-2 Antibody

Anti-BMP-7 Antibody, clone 2A10Anti-BRD2 AntibodyAnti-CACYBP, clone 7D11 AntibodyAnti-CD73 Antibody, clone AA60-E3-3

Anti-CK1, clone 3C10F7 AntibodyAnti-Notch1 Antibody, IntracellularAnti-Cripto-1 AntibodyAnti-CYLD Antibody, clone 16A1Anti-DKK1, clone 2H2 AntibodyAnti-DLK1 AntibodyAnti-Emerin Antibody, clone 7D9Anti-Fascin Antibody, clone 55K2

Anti-FOXC1 AntibodyAnti-FoxD3 AntibodyAnti-FOXK2 Antibody Anti-FoxP1 Antibody

Anti-FoxP2 AntibodyAnti-FOXP3 Antibody, clone 3G3Anti-Frizzled 5 AntibodyAnti-GATA-6 AntibodyAnti-GLI1 Antibody

Anti-GSK-3b Antibody, a.a. 335-349Anti-HES-1 AntibodyAnti-phospho-ITCH (pT222) AntibodyAnti-Jagged2 AntibodyAnti-KIF3A Antibody, clone 4F9.1 Anti-Klf4, clone 10E3.1 AntibodyAnti-Lin28 AntibodyAnti-LIN28 Antibody, clone 6D1F9Anti-Lin28 Antibody, clone PR3G10Anti-LRP6 Antibody, clone 1C10Anti-MAML1 AntibodyAnti-Musashi-1 Antibody

Anti-Nanog AntibodyAnti-NANOG Antibody, clone 7F7.1Anti-Nanog Antibody, NTAnti-phospho-NDRG1 (Ser330), clone EPR5594, Rabbit Monoclonal AntibodyAnti-Nicastrin Antibody

Western BlottingImmunofluorescence;Immunoprecipitation;Western Blotting;ImmunohistochemistryWestern Blotting;Immunoprecipitation;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunohistochemistry

Western Blotting;ImmunohistochemistryImmunohistochemistry

Immunocytochemistry;Flow Cytometry;Western Blotting;ImmunoprecipitationImmunohistochemistryELISA;Western Blotting;Immunohistochemistry (Paraffin)Western BlottingWestern BlottingWestern BlottingWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunoprecipitationImmunocytochemistry;Western Blotting;ImmunohistochemistryWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin)Immunohistochemistry;Western BlottingDot Blot;Western Blotting

Immunohistochemistry (Paraffin);ELISAWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;ImmunoprecipitationWestern Blotting;Immunofluorescence


Western Blotting;ImmunoprecipitationWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Western BlottingFlow Cytometry;Western BlottingWestern Blotting;Immunocytochemistry

Western BlottingWestern Blotting;Immunohistochemistry

Immunoprecipitation;Western Blotting;Immunocytochemistry

HumanHuman;Mouse

Human;Mouse

Human

Human;Mouse

Human;RatHuman

Human

HumanHuman

HumanHumanHumanHuman;Mouse;RatHumanHuman;Mammals;Echinoderms;Gerbil;Sea UrchinHuman;MouseHuman;MouseHumanHuman;Mouse

Mouse;Rabbit;HumanHuman;Mouse;RatHuman;Mouse;RatHumanHuman;Rat

Rat;Human;Mouse;Bovine

Human;MouseHuman

HumanHuman;Mouse;RatHumanHuman;MouseHuman

Human

Human;Rat;MouseHuman;MouseHuman;Mouse;Rat

Human;MouseHuman


Human;Mouse;Rat

100 µL100 µg

100 µg

100 µg

100 µg

100 µg100 µL

100 µg

100 µg200 µL

100 µg100 µg100 µL100 µg100 µg100 µg

100 µg100 µg100 µL100 µL

100 µL100 µL200 µg100 µg100 µL

50 µg

100 µg100 µg

100 µg100 µg100 µg100 µL100 µL

100 µg

100 µg100 µg100 µg

100 µL100 µg

100 µg100 µL

100 µL

53


抗体

ABN40MABE69MAB3582

ABD71AB5687ABE1828ABE68

ABE73ABE7506-756ABE1351ABC217

07-1413

AB5702AB10050

06-1097MABC155MABD3507-1385MABD163

MABD102

MABS341AB5975AB5977

AB9220MABD24

AB5731MABC774

MAB5556

Anti-DLK1 AntibodyAnti-Emerin Antibody, clone 7D9Anti-Fascin Antibody, clone 55K2

Anti-FOXC1 AntibodyAnti-FoxD3 AntibodyAnti-FOXK2 Antibody Anti-FoxP1 Antibody

Anti-FoxP2 AntibodyAnti-FOXP3 Antibody, clone 3G3Anti-Frizzled 5 AntibodyAnti-GATA-6 AntibodyAnti-GLI1 Antibody

Anti-GSK-3b Antibody, a.a. 335-349Anti-HES-1 AntibodyAnti-phospho-ITCH (pT222) AntibodyAnti-Jagged2 AntibodyAnti-KIF3A Antibody, clone 4F9.1 Anti-Klf4, clone 10E3.1 AntibodyAnti-Lin28 AntibodyAnti-LIN28 Antibody, clone 6D1F9Anti-Lin28 Antibody, clone PR3G10Anti-LRP6 Antibody, clone 1C10Anti-MAML1 AntibodyAnti-Musashi-1 Antibody

Anti-Nanog AntibodyAnti-NANOG Antibody, clone 7F7.1Anti-Nanog Antibody, NTAnti-phospho-NDRG1 (Ser330), clone EPR5594, Rabbit Monoclonal AntibodyAnti-Nicastrin Antibody

Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunoprecipitationImmunocytochemistry;Western Blotting;ImmunohistochemistryWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin)Immunohistochemistry;Western BlottingDot Blot;Western Blotting

Immunohistochemistry (Paraffin);ELISAWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;ImmunoprecipitationWestern Blotting;Immunofluorescence


Western Blotting;ImmunoprecipitationWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Western BlottingFlow Cytometry;Western BlottingWestern Blotting;Immunocytochemistry

Western BlottingWestern Blotting;Immunohistochemistry

Immunoprecipitation;Western Blotting;Immunocytochemistry

Human;Mouse;RatHumanHuman;Mammals;Echinoderms;Gerbil;Sea UrchinHuman;MouseHuman;MouseHumanHuman;Mouse

Mouse;Rabbit;HumanHuman;Mouse;RatHuman;Mouse;RatHumanHuman;Rat

Rat;Human;Mouse;Bovine

Human;MouseHuman

HumanHuman;Mouse;RatHumanHuman;MouseHuman

Human

Human;Rat;MouseHuman;MouseHuman;Mouse;Rat

Human;MouseHuman


Human;Mouse;Rat

100 µg100 µg100 µg

100 µg100 µg100 µL100 µL

100 µL100 µL200 µg100 µg100 µL

50 µg

100 µg100 µg

100 µg100 µg100 µg100 µL100 µL

100 µg

100 µg100 µg100 µg

100 µL100 µg

100 µg100 µL

100 µL

07-1232

07-1234

MABC594

MABN281

MABD76

MAB4305MAB4419

MAB4419A4

05-1573

ABN489

ABC478

Anti-Notch 1 Antibody, NT

Anti-Notch 2 Antibody, NT

Anti-NOTCH 3/N3ECD Antibody, clone 1E4Anti-Numb Antibody, clone 6G10.1Anti-Oct-4 Antibody, clone 9B7

Anti-Oct-4 Antibody, clone 9E3.2Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2, Alexa Fluor® 488 conjugateAnti-Pax5 Antibody, clone 1H9

Anti-PEN2 Antibody

Anti-phospho-CYLD Antibody (Ser418)

ELISA; Western Blotting;Immunohistochemistry (Paraffin)ELISA;Western Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunocytochemistry;Electron MicroscopyWestern Blotting;Immunocytochemistry

Western Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;ELISAImmunocytochemistry;Flow Cytometry;Western BlottingImmunocytochemistry

Western Blotting;Immunohistochemistry;ImmunoprecipitationWestern Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting

Human;Mouse

Human;Mouse

Human

Human

Mouse;Human

Human;Rat;MouseHuman;Mouse

Human;Mouse

Mouse;Human

Mouse;Rat

Human

200 µL

200 µL

100 µg

100 µg

100 µL

100 µg100 µg

100 µL

100 µg

100 µg

100 µL

54

MM匹配抗体名MM匹配抗体编号 Key Applications Species Reactivity Qty/Pk

抗体

InhibitorSelect™Wnt Signaling Pathway Inhibitor Panel,目录号681666组分靶标包装量目录号

Casein KinaseⅠInhibitor,D4476Casein KinaseⅡInhibitorⅢ,TBCAβ-Catenin/Tcf Inhibitor,FH535GSK-3 Inhibitor IXPKGⅠInhibitor,Cell-PermeableH-89,DihydrochlorideJNK InhibitorⅡK-252a,Nocardiopsis sp.KN-93LY 294002(5Z)-7-Oxozeaenol,Curvularia sp.PP2RapamycinSB 202190UO126DMSO

Casein KinaseⅠCasein KinaseⅡβ-Catenin,Tcf GSK-3 PKGPKA,Rho KinaseJNK MultipleCaMKPI3-KinaseTAK 1Src FamilymTORp38MEK-

1 mg5 mg10 mg1 mg1 mg1 mg100 µg5 mg1 mg5 mg1 mg1 mg100 µg1 mg1 mg15 mL

218696218710219330361550370655371963420119420298422708440202499610529573553210559388662005KP31817


抑制剂

07-393

ABS481

06-734

05-613

MAB2081

AB575709-706

MABD175

MABE167

AB5603

MAB4343

05-510

ABE598

ABE41405-511

ABD53

ABE596

MAB4381

MABN722

09-162MABD136

Anti-phospho-GSK3α (Ser21) AntibodyAnti-phospho Beta Catenin (Ser675) AntibodyAnti-β-Catenin Antibody

Anti-β-Catenin Antibody, clone 2H4A7Anti-β-Catenin Antibody, clone 5H10

Anti-Presenilin 1 AntibodyAnti-RING1 Antibody

Anti-SHH Antibody, clone 5H4

Anti-SNAI1 Antibody, clone 10H4.1Anti-Sox2 Antibody

Anti-SOX-2 Antibody

Anti-TCF-1 Antibody, clone 7H3

Anti-TCF12 Antibody

Anti-TCF-3 AntibodyAnti-TCF-4 Antibody, clone 6H5-3

Anti-ZEB1 Antibody

Anti-ZEB1 Antibody

Anti-TRA-1-81 Antibody, clone TRA-1-81Anti-WIF1 Antibody, clone 1G5

Anti-Wnt3A AntibodyAnti-Wnt-5a Antibody, clone 6F2

Western Blotting


Immunocytochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;WesternBlotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western BlottingELISA; Western BlottingWestern Blotting

Western Blotting;Flow Cytometry;ImmunohistochemistryWestern Blotting;Immunohistochemistry

Western Blotting;Immunocytochemistry;Immunofluorescence;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Western BlottingImmunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry;ImmunohistochemistryWestern Blotting;ImmunohistochemistryElectrophoretic Mobility Shift Assay;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingWestern Blotting

Western Blotting;Immunocytochemistry;ImmunofluorescenceWestern Blotting;Flow Cytometry;Immunoprecipitation;Immunofluorescence;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunofluorescence;Immunohistochemistry

Human

Human

Human;Rat;Mouse;Rabbit;BovineHuman;Mouse;Rat

Human;Rat

Human;Mouse;RatHuman;Mouse;Horse;Bovine;Pig;CanineHuman;Mouse;Monkey

Rat;Mouse;Human

Human;Mouse

Human;Mouse

Human

Human

HumanHuman;Mouse

Mouse;Human;Bovine;Chicken;FrogHuman

Human

Human;Mouse

Human;Mouse;RatHuman;Rat

100 µL

200 µL

200 µg

200 µg

100 µg

100 µg100 µg

100 µL

100 µg

100 µg

100 µg

100 µg

100 µg

100 µg200 µg

100 µg

100 µg

100 µg

100 µL

100 µL100 µL

55

更多信号通路图及相关研究工具请咨询默克密理博公司或当地代理商

Autophage 信号通路Hedgehog 信号通路 p53 信号通路

IGF 信号通路 FGF 信号通路Wnt 信号通路

MAP 激酶信号通路 VEGF 信号通路NF-κB 信号通路

EGFR 信号通路 JAK/STAT 信号通路PI 3-K/Akt/mTOR 信号通路

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