Cellular immunity during normal pregnancy
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45 CELLULAR IMMUNITY DURING NORMAL PREGNANCY. Takashi Yamamoto, Tadayuki Ishimaru, Koji Nakamura, Nobuyuki Hosoi, Tomoyuki Katahira, Takasi Takagi and Yoshiaki Yagaml Department of Obstetrics and Gynecology, Nagoya City University Medical School, Nagoya, 467, Japan. To investigate a possible role of cellular immunity during normal preg- nancy, monocyte subpopulation,T cell subsets,concanavalin A(Con A) indu- ced suppressor cell activity and natural killer(NK) activity were assessed in peripheral blood of l)normal pregnant women at different stages of gestation and 2)healthy non-pregnant women who were served as control. Monocyte subpopulation and T cell subsets were characterized by indirect immunofluorescence with monoclonal antibody(OKT3,0KT4,0KT8,0KIa l ) and by indirect antiglobulin rossetting reaction(IARR) with OKIa I. Con A induced suppressor cell activity was measured by the suppression of mitogenic proliferation. NK activity was also investigated by~Cr re- lease assay. During the first trimester of pregnancy,a significant increase in the + . . . . percentage of OKT4 T cell and a significant decreas~ in the percentage of OKT8 T cell were observed. The percentage of la monocyte increased significantly during the first and se#ond trimester. The tendency was also observed in the percentage of la- T cell by IARR. Con A induced cell in normal pregnancy suppressed significantly the mitogenic proliferation of both autologous and allogenic peripheral blood lymphocyte. Moreover, NK activity showed a significant decrease during normal pregnancy. Our data suggest that cellular immunity during normal pregnancy may be functionally suppressed via the complicated cell-cell interaction. IMMUNOREGULATORY MECHANISMOF NORMALAND ABNORMAL PREGNANCY IN THE ASPECT OF COMPLEMENT. Ryoki Ohkawa, Kimiyasu Ohkawa, Toshikatu Ohhashi, and Hisaaki Hatano. Dept. of OB.& GYN. Nippon Medical School, I-I-5, Sendagi, Bunkyo-Ku, Tokyo, Japan. Aim of study : In the pregnancy C3-system (alternative pathway) of comple- ment will play a important role as primitive defence system. During pregnancy the cellular immunity come down slightly, and the complement system especially C3-system is enhanced in compensation for it. Method : Skin test by DNCB, PPD, and PHA were examined. The complement activity of classical pathway was measured by Mayer's method (CH50) and that of alternative pathway (AI-CH50) was measured by principle that human alter- native pathway hemolysis the rabbit erythrocytes, the component of complement C3,C3-activator, and C4 were measured by single radial immunodiffusion.these measurement were performed in the 82 cases of normal pregnancy, 115 cases of intrauterin fetal death, 28 cases of hydatidiform mole, 21 cases of toxemia, 5 cases of chorionic malignancy and 3 cases of uteroplacental apoplexia. Results : The aged necrotic villi activated the alternative pathway of complement. In the all cases of abnormal pregnancy with necrotic villi, CH50 and AI-CH50 were came down and C3, C3-activator, and C4 were decreased quanti- tatively, especially uteroplacental apoplexia were markedly decreased. Conclusion : In the abnormal pregnancy with low activity of CH50, AI-CH50, and the decrease of C3, C3-activator, and C4, it considered that necrotic villi presented in the uterin cavity and evacuation would become necessary, these findings suggest to resection by nonspecific immune response.
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Transcript of Cellular immunity during normal pregnancy
45
CELLULAR IMMUNITY DURING NORMAL PREGNANCY. Takashi Yamamoto, Tadayuki Ishimaru, Koji Nakamura, Nobuyuki Hosoi, Tomoyuki Katahira, Takasi Takagi and Yoshiaki Yagaml Department of Obstetrics and Gynecology, Nagoya City Univers i ty Medical School, Nagoya, 467, Japan.
To invest igate a possible role of ce l l u l a r immunity during normal preg- nancy, monocyte subpopulation,T ce l l subsets,concanavalin A(Con A) indu- ced suppressor ce l l a c t i v i t y and natural k i l ler (NK) a c t i v i t y were assessed in peripheral blood of l)normal pregnant women at d i f fe ren t stages of gestation and 2)healthy non-pregnant women who were served as contro l . Monocyte subpopulation and T ce l l subsets were characterized by ind i rec t immunofluorescence with monoclonal antibody(OKT3,0KT4,0KT8,0KIa l ) and by ind i rec t an t ig lobu l in rossett ing reaction(IARR) with OKIa I . Con A induced suppressor ce l l a c t i v i t y was measured by the suppression of mitogenic p ro l i f e ra t i on . NK a c t i v i t y was also invest igated by~Cr re- lease assay.
During the f i r s t t r imester of pregnancy,a s ign i f i can t increase in the + . . . .
percentage of OKT4 T cel l and a s ign i f i can t decreas~ in the percentage of OKT8 T ce l l were observed. The percentage of la monocyte increased s i g n i f i c a n t l y during the f i r s t and se#ond tr imester. The tendency was also observed in the percentage of la- T ce l l by IARR.
Con A induced ce l l in normal pregnancy suppressed s i g n i f i c a n t l y the mitogenic p ro l i f e ra t i on of both autologous and al logenic peripheral blood lymphocyte. Moreover, NK a c t i v i t y showed a s ign i f i can t decrease during normal pregnancy.
Our data suggest that ce l l u l a r immunity during normal pregnancy may be func t i ona l l y suppressed via the complicated c e l l - c e l l in te rac t ion .
IMMUNOREGULATORY MECHANISM OF NORMAL AND ABNORMAL PREGNANCY IN THE ASPECT OF COMPLEMENT.
Ryoki Ohkawa, Kimiyasu Ohkawa, Toshikatu Ohhashi, and Hisaaki Hatano. Dept. of OB.& GYN. Nippon Medical School, I - I - 5 , Sendagi, Bunkyo-Ku, Tokyo, Japan.
Aim of study : In the pregnancy C3-system (a l te rnat ive pathway) of comple- ment w i l l play a important role as pr im i t i ve defence system. During pregnancy the ce l l u l a r immunity come down s l i g h t l y , and the complement system especia l ly C3-system is enhanced in compensation for i t .
Method : Skin test by DNCB, PPD, and PHA were examined. The complement a c t i v i t y of c lassical pathway was measured by Mayer's method (CH50) and that of a l te rnat ive pathway (AI-CH50) was measured by p r inc ip le that human a l te r - nat ive pathway hemolysis the rabbi t erythrocytes, the component of complement C3,C3-activator, and C4 were measured by single radial immunodiffusion.these measurement were performed in the 82 cases of normal pregnancy, 115 cases of i n t rau te r in fe ta l death, 28 cases of hydatidiform mole, 21 cases of toxemia, 5 cases of chorionic malignancy and 3 cases of uteroplacental apoplexia.
Results : The aged necrot ic v i l l i act ivated the a l te rnat ive pathway of complement. In the a l l cases of abnormal pregnancy with necrot ic v i l l i , CH50 and AI-CH50 were came down and C3, C3-act ivator, and C4 were decreased quanti- t a t i v e l y , especia l ly uteroplacental apoplexia were markedly decreased.
Conclusion : In the abnormal pregnancy with low a c t i v i t y of CH50, AI-CH50, and the decrease of C3, C3-act ivator, and C4, i t considered that necrot ic v i l l i presented in the uter in cav i ty and evacuation would become necessary, these f ind ings suggest to resection by nonspecif ic immune response.
