結構生物學期末報告

生技所 蕭孟昌

學號 M93360039

Structure of the DNA-bindingdomains from NFAT, Fos and

Jun bound specifically to DNA

Structure of the DNA-bindingdomains from NFAT, Fos and

Jun bound specifically to DNA Science March 1998 VOL 392 P42-48 Lin Chen*, J. N. Mark Glover*†, Patrick G. Hogan‡, Anj

ana Rao‡ & Stephen C. Harrison*† * Department of Molecular and Cellular Biology and † H

oward Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA‡ Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA

PDB ID :1A02

WHAT QUESTION IS THIS PAPER WANT TO SOLVEWHAT QUESTION IS THIS PAPER WANT TO SOLVE

The interaction between NFAT (nuclear factor of activated T cells) and AP-1(Fos-Jun).

Explain the mechanism of combinatorial regulation of transcription.

WHAT WE CAN DO IN THIS STUDY

WHAT WE CAN DO IN THIS STUDY

What amino acid sequences are conserved and what are these sequences function?

What DNA sequences are conserved and what are these sequences function?

Try to find out other TF has similar mechanism to NFAT and AP-1.

ANALYSIS OF NFAT

ANALYSIS OF NFAT

CONSERVED REGION OF NFAT

CONSERVED REGION OF NFAT

TRP399. SER429( 與 DNA 之間有 INTERANTION) ASN451. THR604( 與 DNA 之間有 INTERANTION) PRO643( 與 AP-1 之間有 INTERACTION) PRO677( 與 AP-1 之間有 INTERACTION)

ANALYSIS OF AP-1

ANALYSIS OF AP-1

CONSERVED REGION OF AP-1

CONSERVED REGION OF AP-1

ALA150( 與 DNA 之間有 INTERACTION) ARG159( 與 NFAT 之間有 INTERACTION) GLU160 ( 與 NFAT 之間有 INTERACTION) SER279( 與 NFAT 和 DNA 之間都有 INTERA

CTION) VAL294(Jun and Fos 的交錯點 )

ANALYSIS OF DNA SEQUENCEANALYSIS OF DNA SEQUENCE

DNA fragment that has the sequence of the distal antigen-receptor response element (ARRE2) from the murine IL-2 promoter23.

分析結果分析結果DNA sequence conserved region 生物上的功能性雖不若 protein sequence 的顯著，但很明顯的和轉錄因子結合的 DNA sequence 仍具有高度的保留性。

AP FAMILYAP FAMILY命運法則

調控基因的能力是否相同？

THANK YOU