蛋白用于三元复合基因载体
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1
DNA
//DNA
DNA
32gHSA
HSAHSADNA
HSADNA
S. Simoes et al. / Biochimica et Biophysica Acta 1463 (2000)
459~469
Transfection by HSA-lipoplexes in vivo. HSA-lipoplexes, plain
lipoplexes (both at a charge ratio (+/3) of 2/1 and containing 100
Wg pCMVluc), or naked DNA were injected into mice via the tail vein
in a volume of 200 Wl. Luciferase gene expression in the lungs and
spleen was measured 8 h following injection, and is given as pg
luciferase/mg organ. Each bar represents a dierent animal. Note
that luciferase expression by naked DNA was too low to be apparent
at this scale.
DNA,
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HOWEVER
DNA
DNADNA
Human serum albuminpolyethylenimine nanoparticles for gene
delivery. Journal of Controlled Release
Effect of different N/P ratios of HSAPEIDNA nanoparticles
(hatched bars) and PEIDNA complexes (black bars) on the viability
of 293 cells. The MTT dye reduction assay was performed 48 h after
incubation of the cells with the same amount of the preparations as
in the transfection trials.
N/PHSA/PEI/DNAPEI/DNA80%
Human serum albuminpolyethylenimine nanoparticles for gene
delivery. Journal of Controlled Release
HSA
J Gene Med 2005; 7: 15551564.
HSA enhances PEI-mediated transfection. 9HTEo-(A) and A549 (B)
cells were plated onto 24-well plates at 100 000and 50 000 per
well, respectively, to obtain 90100% confluency after 2 days of
culture. Cells were incubated with 1 g of pCLuc complexed with PEI
(10N/P) with HSA over a range of albumin amounts (expressed in g on
the x-axis) in a total volume of 1 ml. Twenty-four hours after
transfection, cells were lysed and cell lysates were tested for
luciferase activity, expressed as relative light units (RLU) per g
of protein content. Values represent the mean (SEM) of three
experiments conducted in duplicate
Biomacromolecules 2011, 12, 10061014
BSA/PDMA/DNA50nm
BSA-PDMABSABSA-PDMA
BSA
N/P
N/P
PVPPDMAEMAPPDN/P3PPDDNAPPD/DNAPVPDNADNADNA
B. Zhang et al. / Journal of Biomaterials Science 0 (2012)
116
BSA/PPD/DNAHepG2BSA
Cytotoxicity and transfection efficiency in vitro. (a) Cell
viabilities of HepG2 cells treated by BSA/PPD-1/pDNAcomplexes (the
N/P ratio of the PPD-1/pDNA binary complexes is 15) with the
increase of BSA concentrations determined by MTT assay. Experiments
were performed in triplicate; values represent the relative
viability compared to untreated cells as means SEM of one
representative experiment (n = 3). (b) The transfection
efficiencies of BSA/PPD-1/pDNA complexes (the N/P ratio of the
PPD-1/pDNA binary complexes is 15) with the increase of BSA
concentrations in HepG2 cells determined by flow cytometry.
Experiments were performed in triplicate; values represent the
EGFP-positive cells as means SEM of one representative experiment
(n = 3). (c) Fluorescence images of HepG2 cells. (c1, c2)
PPD-1/pDNA binary complexes at a N/P ratio of 15 and BSA/PPD-1/pDNA
ternary complexes (the concentration of BSA is 7.5 g/ml),
respectively.
10%HepG2BSA/PPD/DNAPEI
BSADNA
PEI/DNANIH/3T3/DNA
Journal of Nanoscience and Nanotechnology, 2010, 10(5):
3170-3174.
/DNADNA