Title セラミック水の Streptococcus mutans におけるう蝕原性抑制効果の検討

Author(s) 米山, 莉紗

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URL https://doi.org/10.18910/72249

DOI 10.18910/72249

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Note

Osaka University Knowledge Archive : OUKAOsaka University Knowledge Archive : OUKA

https://ir.library.osaka-u.ac.jp/repo/ouka/all/

Osaka University

Streptococcus mutans

1

6074-

-

Streptococcus mutans

Hamada Slade, 1980 S. mutans

, 1997 S. mutans

190 kDa Protein

antigen PA Okahashi , 1989

S. mutans 3 Glucosyltransferase ; GTF GTFB

GTFCGTFD

Ooshima , 2001 S. mutans

Ooshima , 2001 S. mutans

Glucan-binding protein ; Gbp GTF

S. mutans Banas

, 2003

S. mutans

Hamada Slade, 1980Hamada , 1984

Cnm Cbm

CBP S. mutans

Nomura , 2012

Smith , 2002

Matsumoto , 1999Signoretto , 2010

Smith , 2002

Gluzman , 2013

2

He , 2015

β-TCP

Myoui , 2006

3,000

, 2016

S. mutans

in vitro S. mutans

-

-

-

3

��3��-

-

LK ?GE@=>5��

1

3,000

wt% 76 : 15 : 9

2 100 mg

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0.1 ppm 3

H2O

45 NaCl

NaCl 5.0 wt %

10 6

S. mutans

8

MK ��/2%�

S. mutans MT8148

c CBP PA Ooshima 1983

SA31 k Cbm PA Nakano 2008

Streptococcus sobrinus 6715 g Ooshima 1993

Brain Heart Infusion BHI; Difco Laboratories Detroit, MO, USA

Todd Hewitt TH; Difco Laboratories Mitis Salivarius MS

Difco Laboratories, Detroit, MO, USA 0.2 U/mL; Sigma-Aldrich

Co., St. Louis, MO, USA 15 % w/v MSB

BHI TH 37 18

MSB 95 % 5% 37 48

4

NK �%��4,18��

Sasaki 2004 10 mL

BHI 37 18 MT8148 SA31 6715

3,000 rpm 10

Phosphate buffered saline; PBS OD550 = 1.0

1.0×109 CFU/mL

37 5 15 30 45 1.5 3 6

12 1 ppm10 ppm

100 ppm MSB

37 48 %

S. mutans /

S. mutans 5

OK ������4,18��

Sasaki 2004 10 mL

BHI TH 37 18 MT8148

SA31 OD550 = 1.0 1.0×109 CFU/mL

1.0×105 CFU

100 ppm 37 3

6 12 MSB

37 48

% S. mutans /

S. mutans

5

PK >=IJ>�� ��#418����5��

Kawabata Hamada 1999

10 mL BHI 37 18 MT8148

SA31 6715 3,000 rpm 10

1% BHI BHI

5

1/10 BHI 1.0×107 CFU/ml 30°

37 18

3

3 mL

Libra S12, Biochrom Ltd, Cambridge, UK

OD550 %

OD550 / OD550 5

QK B;<D:HF��#418����5��

Kuboniwa 2006

10 mL BHI 37 18 MT8148

3,000 rpm 10 10 mM Hexidium Iodide

Molecular Probes, Eugene, OR, USA 5 µL 1 mL

15 100 ppm

0.25 % BHI BHI

1/10 BHI OD600 0.1 1

60 30 12,000 rpm 1

25 %

Chambered Coverglass System CultureWell™,Grace Bio Lab, Bend, OR, USA 150

µL 37 2

200 µL 37

24 PBS 100 µL

488 nm

TSC-SP5 Leica Microsystems GmbH, Wetzlar, Germany

DMI6000B Leica 63×

Image J version 1.34s National Institutes of Health, Bethesda, MD, USA

Ardin 2014 10

mL BHI 37 18 MT8148 6715

3,000 rpm 10 100 ppm

0.25 % BHI OD600 0.1

6

60 30

12,000 rpm 1

25 % 0.45 µL

96 Corning Inc., Corning, NY, USA 200 µL

37 2

200 µL 37 24

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PBS

95 %

Thermo Fisher Scientific, Waltham, MA, USA OD595

5

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41 17 24 18 37 22.1

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27 13 14 18 37

23.2

Ooshima 1994

MSB 37 48

Quiugley Hein

1962 Red-

Cote, John O.Butler Co., Chicago, IL, USA

0 3 4

2

1 10

7

1

1

S. mutans

SK !'��

GraphPad Prism 6 GraphPad Software Inc., La Jolla, CA, USA

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5 %

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1.0×109 CFU/mL S. mutans MT8148 100 ppm

1.5 5 % 3

8 PBS 1.5

9 S. mutans SA31

1.5

3 20 % 6

0 % 10 PBS

1.5 10 %

11

MT8148 5 50 %

90 12

MT8148 1.5

10 ppm P 0.05 13 S. sobrinus

6715 5

3 0 % 14

MK ������4,18���100 ppm BHI 1.0×105 CFU MT8148

SA31 3 6 12

S. mutans

1516 TH

S. mutans 1718

NK>=IJ>�� ��#418����5��

BHI 1 % BHI 1.0×107 CFU/mL

MT8148 100 ppm

19

BHI 1/10 1 % BHI

P

9

0.001 20 6715 BHI

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2122

OKB;<D:HF��#418����5��

BHI 0.25 %

BHI MT8148 100 ppm

MT8148

23 BHI 1/10

BHI MT8148

6715

P 0.001 2425

PK.&����4,18$���

1

2627 100 ppm

1

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S. mutans 1 S. mutans

S. mutans

P 0.05 29

-

-

-

-

10

"-

-

TiC

64:36 1 0.08 ppm

, 2016 TiO2

Lu , 2003

Yao , 2008

Burne

, 1987

Burne , 1987Bellantone , 2002Hipler ,

2006 S. mutans

Burne , 1987

Castillo ,

2011

Kawasita , 2000

H2O2 UV

Porphyromonas gingivalis Fusobacterium nucleatum S. mutans

Feuerstein , 2006Ikai , 2013

11

MT8148 SA 31 MT8148

S. mutans

c/e/f/k

Hamada , 1980Nakano , 2004 S. sobrinus

d/g Okahashi , 1986 MT8148

SA31 c k

MT8148

SA31

Nomura , 2012 SA 31 MT8148

S. mutans

SA31 PBS

PBS

0.1 ppm

NaCl

5.0 wt % 1 ppm

Cl- AgCl

NaCl

10 SA31

PBS

, 2016

PBS

BHI

S. mutans S. sobrinus

12

S. mutans S. sobrinus

BHI

BHI

BHI

S. mutans

99.5 %

0.5 % Na+ K+

Ca2+ Cl-HCO3- Hofman

, 2001Kaufman , 2002

S. mutans

Smith , 2002

Ooshima , 1994

S. mutans S. mutans

S.

mutans Ooshima , 1994

S. mutans

S. mutans

Hamada , 1984

Moriwaki , 2011

13

TiC

BHI

S. mutans

S. mutans

14

(-

PBS

S. mutans

BHI S. mutans

S. mutans

S. mutans

15

)+-

16

��-

-

, . 1997. .

. , , , , , pp.156-

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