Night session

Pat Pataranutaporn (P.P.) JSTP #12

MISSION IMPOSSIBLE

Night session

Pat Pataranutaporn (P.P.) JSTP #12

MISSION IMPOSSIBLE

โครงงานของฉันและประสบการณ์

Present งานต่างแดน

IMPOSSIBLEMISSION

IMPOSSIBLEMISSION

IMPOSSIBLE PROJECT

FamilyJSTP

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“ Everyone has a question.

What's yours? “

“ Everyone has a question.

What's yours? “

“ Never be afraid to asked “

ZOMBIE

Dr.Atichart Ketsuwan and his reseach that use mathematics to study the history of

ancient temple

วัดอุโมง,เชียงให

ม่

NatureThe hidden treasure

Biomimicry

“ Never be afraid to asked “

IMPOSSIBLE

IMPOSSIBLEIS NOTHING

IMPOSSIBLE

IMPOSSIBLE

IMPOSSIBLE

Right solution

POLYSTYRENE

Styromelt™,http://www.polystyrenerecycle.com/

100,000 Tons

Styromelt™,http://www.polystyrenerecycle.com/

of Polystyrene was produce in UK.

“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.

"Ease of Disposal". Retrieved 2009-06-25

“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.

"Ease of Disposal". Retrieved 2009-06-25

CARCINOGEN

Styrene Monomer

Carbon Monoxide

“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.

"Ease of Disposal". Retrieved 2009-06-25

CARCINOGEN

Styrene Monomer

Carbon Monoxide

“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.

"Ease of Disposal". Retrieved 2009-06-25

CARCINOGEN

Styrene Monomer

Carbon Monoxide 1%RECYCLE

Information & Communication Technology Center, Ministry of Energy, THAILAND

Natural degradation

>500 years

Information & Communication Technology Center, Ministry of Energy, THAILAND

Natural degradation

>500 years

My inspiration

My inspiration

My inspiration

My inspiration

My inspiration

My inspiration

สิ่งเล็กๆที่เรียกว่าแบคทีเรีย

สิ่งเล็กๆที่เรียกว่าแบคทีเรีย

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WHY PS foam

spend long time in degradation ?

chemical formula is (C8H8)n

Polymer of styrene

Hydrogen

Carbon

organic compound

s

organic compound

s

organic compound

s

Naturally

POLYSTYRENE

organic compound

s

organic compound

s

organic compound

s

Naturally

POLYSTYRENE

organic compound

s

organic compound

s

organic compound

s

Naturally

POLYSTYRENE

organic compound

s

organic compound

s

organic compound

s

Naturally

POLYSTYRENE

organic compound

s

organic compound

s

organic compound

s

Naturally

HOW we isolate the bacteria that able to

degrade foam ?

If bacteria can degrade foam, how it utilize ?

chemical formula is (C8H8)n

Polymer of styrene

Hydrogen

Carbon

Bacteria nutritional requirements

‣ Energy source ‣ Carbon source ‣ Nitrogen source ‣ Minerals ‣ Water ‣ Growth factors

HOW we isolate the bacteria that able to

degrade foam ?

POLYSTYRENE

Isolation of polystyrene foam biodegradable bacteria from petrol contaminated soil

Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang

Abstract 01 The purpose of this study was to isolate polystyrene foam biodegradable bacteria from petrol contaminated soil. Mineral salt medium with polystyrene foam as the only carbon source was used in this experiment. After 4 months of incubation, surface of polystyrene foam was observed compared with the control group. The result showed that there were some traces of degradation on the surface of polystyrene foam when compared to the control group which the same source of soil was used as inoculum but had been sterilized before inoculation. Twenty-four isolates of Gram-positive bacteria were isolated from polystyrene foam degraded flasks, 19 isolates were bacilli and 5 were cocci shape.

Introduction 02 Polystyrene foam is a type of plastic produced from styrene. It is a lightweight, moisture-resistant material with exceptional insulation properties. Products made from foamed polystyrene are nearly ubiquitous, for example packing materials, insulation, and foam drink cups. Polystyrene takes hundreds of years to decompose and is often abundant as a form of pollution in the outdoor environment.

Methodology 03 Methodology. Soil samples were collected from sources that were contaminated by petrol. One gram of contaminated soil sample was inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4, 0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg; NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask. Small pieces of 0.1 g of polystyrene foam were added as carbon source. Prior to use, polystyrene foam was soaked in 70% alcohol and washed two times with distilled water and dried in desiccator for two days. In the control group, 1 gram of sterilized petrol contaminated soil sample was used. All experiment was carried out in triplicate. All flasks were incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of incubation 1 ml of cell suspension was inoculated into a new flask and incubated in the same condition for 2 months. Surface of polystyrene foam was observed under stereo zoom microscope and taken photograph. Culture broth was diluted and spread onto glucose yeast extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria were picked, purified and morphological studied by Gram-staining and observed under microscope.

Result and discussion 04 The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape. Furthermore, some traces of degradation were found on the surface of polystyrene foam from the experiment flasks rather than the control group. These results indicated that there are polystyrene foam degradable bacteria from petrol contaminated soil and It might use polystyrene foam as carbon and energy sources. However, further investigation need to be done for confirmation the results of these experiments and that is our future study.

Acknowledgement: This project was supported by JSTP. National Science and Technology Development Agency.

Experimental group Control group

Reference 05 Daniel Burd (2008) Plastic not fantastic. Libraries (online). Available : http://www.science.uwaterloo.ca

Rod shape Positive gram

Round shape Positive gram

79.1% (19 isolates )

20.9% (5 isolates )

Types of bacteria that isolated from the experimental group

The surface of polystyrene foam

Isolation of polystyrene foam biodegradable bacteria from petrol contaminated soil

Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang

Abstract 01 The purpose of this study was to isolate polystyrene foam biodegradable bacteria from petrol contaminated soil. Mineral salt medium with polystyrene foam as the only carbon source was used in this experiment. After 4 months of incubation, surface of polystyrene foam was observed compared with the control group. The result showed that there were some traces of degradation on the surface of polystyrene foam when compared to the control group which the same source of soil was used as inoculum but had been sterilized before inoculation. Twenty-four isolates of Gram-positive bacteria were isolated from polystyrene foam degraded flasks, 19 isolates were bacilli and 5 were cocci shape.

Introduction 02 Polystyrene foam is a type of plastic produced from styrene. It is a lightweight, moisture-resistant material with exceptional insulation properties. Products made from foamed polystyrene are nearly ubiquitous, for example packing materials, insulation, and foam drink cups. Polystyrene takes hundreds of years to decompose and is often abundant as a form of pollution in the outdoor environment.

Methodology 03 Methodology. Soil samples were collected from sources that were contaminated by petrol. One gram of contaminated soil sample was inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4, 0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg; NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask. Small pieces of 0.1 g of polystyrene foam were added as carbon source. Prior to use, polystyrene foam was soaked in 70% alcohol and washed two times with distilled water and dried in desiccator for two days. In the control group, 1 gram of sterilized petrol contaminated soil sample was used. All experiment was carried out in triplicate. All flasks were incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of incubation 1 ml of cell suspension was inoculated into a new flask and incubated in the same condition for 2 months. Surface of polystyrene foam was observed under stereo zoom microscope and taken photograph. Culture broth was diluted and spread onto glucose yeast extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria were picked, purified and morphological studied by Gram-staining and observed under microscope.

Result and discussion 04 The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape. Furthermore, some traces of degradation were found on the surface of polystyrene foam from the experiment flasks rather than the control group. These results indicated that there are polystyrene foam degradable bacteria from petrol contaminated soil and It might use polystyrene foam as carbon and energy sources. However, further investigation need to be done for confirmation the results of these experiments and that is our future study.

Acknowledgement: This project was supported by JSTP. National Science and Technology Development Agency.

Experimental group Control group

Reference 05 Daniel Burd (2008) Plastic not fantastic. Libraries (online). Available : http://www.science.uwaterloo.ca

Rod shape Positive gram

Round shape Positive gram

79.1% (19 isolates )

20.9% (5 isolates )

Types of bacteria that isolated from the experimental group

The surface of polystyrene foam

Isolation of polystyrene foam biodegradable bacteria from petrol contaminated soil

Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang

Abstract 01 The purpose of this study was to isolate polystyrene foam biodegradable bacteria from petrol contaminated soil. Mineral salt medium with polystyrene foam as the only carbon source was used in this experiment. After 4 months of incubation, surface of polystyrene foam was observed compared with the control group. The result showed that there were some traces of degradation on the surface of polystyrene foam when compared to the control group which the same source of soil was used as inoculum but had been sterilized before inoculation. Twenty-four isolates of Gram-positive bacteria were isolated from polystyrene foam degraded flasks, 19 isolates were bacilli and 5 were cocci shape.

Introduction 02 Polystyrene foam is a type of plastic produced from styrene. It is a lightweight, moisture-resistant material with exceptional insulation properties. Products made from foamed polystyrene are nearly ubiquitous, for example packing materials, insulation, and foam drink cups. Polystyrene takes hundreds of years to decompose and is often abundant as a form of pollution in the outdoor environment.

Methodology 03 Methodology. Soil samples were collected from sources that were contaminated by petrol. One gram of contaminated soil sample was inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4, 0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg; NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask. Small pieces of 0.1 g of polystyrene foam were added as carbon source. Prior to use, polystyrene foam was soaked in 70% alcohol and washed two times with distilled water and dried in desiccator for two days. In the control group, 1 gram of sterilized petrol contaminated soil sample was used. All experiment was carried out in triplicate. All flasks were incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of incubation 1 ml of cell suspension was inoculated into a new flask and incubated in the same condition for 2 months. Surface of polystyrene foam was observed under stereo zoom microscope and taken photograph. Culture broth was diluted and spread onto glucose yeast extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria were picked, purified and morphological studied by Gram-staining and observed under microscope.

Result and discussion 04 The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape. Furthermore, some traces of degradation were found on the surface of polystyrene foam from the experiment flasks rather than the control group. These results indicated that there are polystyrene foam degradable bacteria from petrol contaminated soil and It might use polystyrene foam as carbon and energy sources. However, further investigation need to be done for confirmation the results of these experiments and that is our future study.

Acknowledgement: This project was supported by JSTP. National Science and Technology Development Agency.

Experimental group Control group

Reference 05 Daniel Burd (2008) Plastic not fantastic. Libraries (online). Available : http://www.science.uwaterloo.ca

Rod shape Positive gram

Round shape Positive gram

79.1% (19 isolates )

20.9% (5 isolates )

Types of bacteria that isolated from the experimental group

The surface of polystyrene foam

Experimental group

control group

The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape.

How to make sure that

bacteria that was isolate can survive in our condition ?

Molecular biology technic

Molecular biology technic

time(week)

Molecular biology technic

time(week)

Microbe diversity

Molecular biology technic

time(week)

Microbe diversity

Dominant species

ControlTreatment

100x 200x 500x

Polystyrene in Medium with Soil sample

regular polystyrene foam that didn’t use in experiment.

Magnification = 300X

Control Experimental group

WHY PS foam spend long time in degradation ?

HOW TO FIND THE MICROBE THAT ABLE TO DEGRADE PS FOAM ?

HOW we isolate the bacteria that able to degrade foam ?

If bacteria can degrade foam, how it

utilize ?

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Question

Question

No great discovery was ever made without a bold guess  Isaac Newton

Start

Goal

Start

Goal

Why

WhyWhy

Start

Goal

Why

Why

Why

Why

Why

Why

Start

Goal

Why

Why

Why

Why

Why

Why

osamu shimomura Wins Nobel Prize in Chemistry 2008

for Discovery Of Green Fluorescent Protein

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You have a chance to answer your own question .

“ Never be afraid to asked “

POSSIBLEIM

AKE POSSIBLEI M

THANK YOU

Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol((Current(advisor)(

Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang(

Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol((Current(advisor)(

Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang(

Advisor