Mission impossible
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Transcript of Mission impossible
Night session
Pat Pataranutaporn (P.P.) JSTP #12
MISSION IMPOSSIBLE
Night session
Pat Pataranutaporn (P.P.) JSTP #12
MISSION IMPOSSIBLE
โครงงานของฉันและประสบการณ์
Present งานต่างแดน
IMPOSSIBLEMISSION
IMPOSSIBLEMISSION
IMPOSSIBLE PROJECT
FamilyJSTP
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“ Everyone has a question.
What's yours? “
“ Everyone has a question.
What's yours? “
“ Never be afraid to asked “
ZOMBIE
Dr.Atichart Ketsuwan and his reseach that use mathematics to study the history of
ancient temple
วัดอุโมง,เชียงให
ม่
NatureThe hidden treasure
Biomimicry
“ Never be afraid to asked “
IMPOSSIBLE
IMPOSSIBLEIS NOTHING
IMPOSSIBLE
IMPOSSIBLE
IMPOSSIBLE
Right solution
POLYSTYRENE
Styromelt™,http://www.polystyrenerecycle.com/
100,000 Tons
Styromelt™,http://www.polystyrenerecycle.com/
of Polystyrene was produce in UK.
“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25
“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25
CARCINOGEN
Styrene Monomer
Carbon Monoxide
“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25
CARCINOGEN
Styrene Monomer
Carbon Monoxide
“Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25
CARCINOGEN
Styrene Monomer
Carbon Monoxide 1%RECYCLE
Information & Communication Technology Center, Ministry of Energy, THAILAND
Natural degradation
>500 years
Information & Communication Technology Center, Ministry of Energy, THAILAND
Natural degradation
>500 years
My inspiration
My inspiration
My inspiration
My inspiration
My inspiration
My inspiration
สิ่งเล็กๆที่เรียกว่าแบคทีเรีย
สิ่งเล็กๆที่เรียกว่าแบคทีเรีย
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WHY PS foam
spend long time in degradation ?
chemical formula is (C8H8)n
Polymer of styrene
Hydrogen
Carbon
organic compound
s
organic compound
s
organic compound
s
Naturally
POLYSTYRENE
organic compound
s
organic compound
s
organic compound
s
Naturally
POLYSTYRENE
organic compound
s
organic compound
s
organic compound
s
Naturally
POLYSTYRENE
organic compound
s
organic compound
s
organic compound
s
Naturally
POLYSTYRENE
organic compound
s
organic compound
s
organic compound
s
Naturally
HOW we isolate the bacteria that able to
degrade foam ?
If bacteria can degrade foam, how it utilize ?
chemical formula is (C8H8)n
Polymer of styrene
Hydrogen
Carbon
Bacteria nutritional requirements
‣ Energy source ‣ Carbon source ‣ Nitrogen source ‣ Minerals ‣ Water ‣ Growth factors
HOW we isolate the bacteria that able to
degrade foam ?
POLYSTYRENE
Isolation of polystyrene foam biodegradable bacteria from petrol contaminated soil
Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang
Abstract 01 The purpose of this study was to isolate polystyrene foam biodegradable bacteria from petrol contaminated soil. Mineral salt medium with polystyrene foam as the only carbon source was used in this experiment. After 4 months of incubation, surface of polystyrene foam was observed compared with the control group. The result showed that there were some traces of degradation on the surface of polystyrene foam when compared to the control group which the same source of soil was used as inoculum but had been sterilized before inoculation. Twenty-four isolates of Gram-positive bacteria were isolated from polystyrene foam degraded flasks, 19 isolates were bacilli and 5 were cocci shape.
Introduction 02 Polystyrene foam is a type of plastic produced from styrene. It is a lightweight, moisture-resistant material with exceptional insulation properties. Products made from foamed polystyrene are nearly ubiquitous, for example packing materials, insulation, and foam drink cups. Polystyrene takes hundreds of years to decompose and is often abundant as a form of pollution in the outdoor environment.
Methodology 03 Methodology. Soil samples were collected from sources that were contaminated by petrol. One gram of contaminated soil sample was inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4, 0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg; NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask. Small pieces of 0.1 g of polystyrene foam were added as carbon source. Prior to use, polystyrene foam was soaked in 70% alcohol and washed two times with distilled water and dried in desiccator for two days. In the control group, 1 gram of sterilized petrol contaminated soil sample was used. All experiment was carried out in triplicate. All flasks were incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of incubation 1 ml of cell suspension was inoculated into a new flask and incubated in the same condition for 2 months. Surface of polystyrene foam was observed under stereo zoom microscope and taken photograph. Culture broth was diluted and spread onto glucose yeast extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria were picked, purified and morphological studied by Gram-staining and observed under microscope.
Result and discussion 04 The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape. Furthermore, some traces of degradation were found on the surface of polystyrene foam from the experiment flasks rather than the control group. These results indicated that there are polystyrene foam degradable bacteria from petrol contaminated soil and It might use polystyrene foam as carbon and energy sources. However, further investigation need to be done for confirmation the results of these experiments and that is our future study.
Acknowledgement: This project was supported by JSTP. National Science and Technology Development Agency.
Experimental group Control group
Reference 05 Daniel Burd (2008) Plastic not fantastic. Libraries (online). Available : http://www.science.uwaterloo.ca
Rod shape Positive gram
Round shape Positive gram
79.1% (19 isolates )
20.9% (5 isolates )
Types of bacteria that isolated from the experimental group
The surface of polystyrene foam
Isolation of polystyrene foam biodegradable bacteria from petrol contaminated soil
Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang
Abstract 01 The purpose of this study was to isolate polystyrene foam biodegradable bacteria from petrol contaminated soil. Mineral salt medium with polystyrene foam as the only carbon source was used in this experiment. After 4 months of incubation, surface of polystyrene foam was observed compared with the control group. The result showed that there were some traces of degradation on the surface of polystyrene foam when compared to the control group which the same source of soil was used as inoculum but had been sterilized before inoculation. Twenty-four isolates of Gram-positive bacteria were isolated from polystyrene foam degraded flasks, 19 isolates were bacilli and 5 were cocci shape.
Introduction 02 Polystyrene foam is a type of plastic produced from styrene. It is a lightweight, moisture-resistant material with exceptional insulation properties. Products made from foamed polystyrene are nearly ubiquitous, for example packing materials, insulation, and foam drink cups. Polystyrene takes hundreds of years to decompose and is often abundant as a form of pollution in the outdoor environment.
Methodology 03 Methodology. Soil samples were collected from sources that were contaminated by petrol. One gram of contaminated soil sample was inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4, 0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg; NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask. Small pieces of 0.1 g of polystyrene foam were added as carbon source. Prior to use, polystyrene foam was soaked in 70% alcohol and washed two times with distilled water and dried in desiccator for two days. In the control group, 1 gram of sterilized petrol contaminated soil sample was used. All experiment was carried out in triplicate. All flasks were incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of incubation 1 ml of cell suspension was inoculated into a new flask and incubated in the same condition for 2 months. Surface of polystyrene foam was observed under stereo zoom microscope and taken photograph. Culture broth was diluted and spread onto glucose yeast extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria were picked, purified and morphological studied by Gram-staining and observed under microscope.
Result and discussion 04 The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape. Furthermore, some traces of degradation were found on the surface of polystyrene foam from the experiment flasks rather than the control group. These results indicated that there are polystyrene foam degradable bacteria from petrol contaminated soil and It might use polystyrene foam as carbon and energy sources. However, further investigation need to be done for confirmation the results of these experiments and that is our future study.
Acknowledgement: This project was supported by JSTP. National Science and Technology Development Agency.
Experimental group Control group
Reference 05 Daniel Burd (2008) Plastic not fantastic. Libraries (online). Available : http://www.science.uwaterloo.ca
Rod shape Positive gram
Round shape Positive gram
79.1% (19 isolates )
20.9% (5 isolates )
Types of bacteria that isolated from the experimental group
The surface of polystyrene foam
Isolation of polystyrene foam biodegradable bacteria from petrol contaminated soil
Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang
Abstract 01 The purpose of this study was to isolate polystyrene foam biodegradable bacteria from petrol contaminated soil. Mineral salt medium with polystyrene foam as the only carbon source was used in this experiment. After 4 months of incubation, surface of polystyrene foam was observed compared with the control group. The result showed that there were some traces of degradation on the surface of polystyrene foam when compared to the control group which the same source of soil was used as inoculum but had been sterilized before inoculation. Twenty-four isolates of Gram-positive bacteria were isolated from polystyrene foam degraded flasks, 19 isolates were bacilli and 5 were cocci shape.
Introduction 02 Polystyrene foam is a type of plastic produced from styrene. It is a lightweight, moisture-resistant material with exceptional insulation properties. Products made from foamed polystyrene are nearly ubiquitous, for example packing materials, insulation, and foam drink cups. Polystyrene takes hundreds of years to decompose and is often abundant as a form of pollution in the outdoor environment.
Methodology 03 Methodology. Soil samples were collected from sources that were contaminated by petrol. One gram of contaminated soil sample was inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4, 0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg; NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask. Small pieces of 0.1 g of polystyrene foam were added as carbon source. Prior to use, polystyrene foam was soaked in 70% alcohol and washed two times with distilled water and dried in desiccator for two days. In the control group, 1 gram of sterilized petrol contaminated soil sample was used. All experiment was carried out in triplicate. All flasks were incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of incubation 1 ml of cell suspension was inoculated into a new flask and incubated in the same condition for 2 months. Surface of polystyrene foam was observed under stereo zoom microscope and taken photograph. Culture broth was diluted and spread onto glucose yeast extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria were picked, purified and morphological studied by Gram-staining and observed under microscope.
Result and discussion 04 The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape. Furthermore, some traces of degradation were found on the surface of polystyrene foam from the experiment flasks rather than the control group. These results indicated that there are polystyrene foam degradable bacteria from petrol contaminated soil and It might use polystyrene foam as carbon and energy sources. However, further investigation need to be done for confirmation the results of these experiments and that is our future study.
Acknowledgement: This project was supported by JSTP. National Science and Technology Development Agency.
Experimental group Control group
Reference 05 Daniel Burd (2008) Plastic not fantastic. Libraries (online). Available : http://www.science.uwaterloo.ca
Rod shape Positive gram
Round shape Positive gram
79.1% (19 isolates )
20.9% (5 isolates )
Types of bacteria that isolated from the experimental group
The surface of polystyrene foam
Experimental group
control group
The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape.
How to make sure that
bacteria that was isolate can survive in our condition ?
Molecular biology technic
Molecular biology technic
time(week)
Molecular biology technic
time(week)
Microbe diversity
Molecular biology technic
time(week)
Microbe diversity
Dominant species
ControlTreatment
100x 200x 500x
Polystyrene in Medium with Soil sample
regular polystyrene foam that didn’t use in experiment.
Magnification = 300X
Control Experimental group
WHY PS foam spend long time in degradation ?
HOW TO FIND THE MICROBE THAT ABLE TO DEGRADE PS FOAM ?
HOW we isolate the bacteria that able to degrade foam ?
If bacteria can degrade foam, how it
utilize ?
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Question
Question
No great discovery was ever made without a bold guess Isaac Newton
Start
Goal
Start
Goal
Why
WhyWhy
Start
Goal
Why
Why
Why
Why
Why
Why
Start
Goal
Why
Why
Why
Why
Why
Why
osamu shimomura Wins Nobel Prize in Chemistry 2008
for Discovery Of Green Fluorescent Protein
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You have a chance to answer your own question .
“ Never be afraid to asked “
POSSIBLEIM
AKE POSSIBLEI M
THANK YOU
Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol((Current(advisor)(
Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang(
Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol((Current(advisor)(
Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang(
Advisor