28/4/2015 LossofGATA3inbladdercancerpromotescellmigrationandinvasion.PubMedNCBI

http://www.ncbi.nlm.nih.gov/pubmed/24448324# 1/2

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CancerBiolTher.2014Apr15(4):42835.doi:10.4161/cbt.27631.Epub2014Jan21.

LossofGATA3inbladdercancerpromotescellmigrationandinvasion.LiY ,IshiguroH ,KawaharaT ,KashiwagiE ,IzumiK ,MiyamotoH .

AbstractThetranscriptionfactorGATA3isknownasabreasttumorsuppressoraswellasaurothelialmarker,anditslossisoftenseeninhighgradeinvasivebladdercancer.Nonetheless,GATA3functionsinbladdercancercellsremainlargelyunknown.Inthisstudy,weassessedtheeffectsofGATA3silencingviaRNAinterferenceoncellmigration,invasion,andproliferationofbladdercancer.GATA3expressionwasdownregulatedinallfourbladdercancerlinesexamined,comparedwithanonneoplasticurotheliallineSVHUC.KnockdownofGATA3inthebladdercancerlines(5637,TCCSUP,J82)resultedinpromotionofcellmigrationandinvasionaswellasincreasesintheexpressionoftheirrelatedmolecules,suchasvascularendothelialgrowthfactor,matrixmetalloproteinase(MMP)2,andMMP9,andtheactivityofMMP2andMMP9.GATA3losswasalsoassociatedwithanincreasinglevelofamesenchymalmarkerNcadherinandadecreasinglevelofanepithelialmarkercatenin.Consistentwiththesefindings,enforcedexpressionofGATA3inUMUC3inhibitedcellmigrationandinvasion.However,GATA3showedmarginaleffectsonbladdercancercellviabilityandtheexpressionofcellcycleorapoptosisrelatedmolecules.Additionally,incontrasttobladdercancerlines,nosignificanteffectsofGATA3silencingoncellmigrationwereseeninSVHUC.ThesefindingssuggestthatGATA3playsanimportantroleinthepreventionofbladdercancerprogressionandmetastasisbyinhibitingcellmigrationandinvasionaswellasepithelialtomesenchymaltransition.

GATA3bladdercancercellinvasioncellmigrationcellproliferation

PMID:24448324[PubMedindexedforMEDLINE] PMCID:PMC3979820 FreePMCArticle

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28/4/2015 LossofGATA3inbladdercancerpromotescellmigrationandinvasion.PubMedNCBI

http://www.ncbi.nlm.nih.gov/pubmed/24448324# 2/2

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