專題討論題目 : RAPD polymorphisms in spring wheat cultiv

ars and lines with different level of Fusarium resistance

作者 : G. Sun, M. Bond, H. Nass, R. Martin and Z. Dong

出處 : Theor Appl Genet (2002)

指導教授 : 王裕文老師

報告學生 : 蔡依真

Fusarium spp. 鐮胞菌Fusarium head blight (FHB)

小麥 大麥

Fusarium head blight

FHB resistance

• resistance to primary infection

• resistance to subsequent colonization after infection

• resistance expressed in the developing kernel

• reduced accumulation of mycotoxins

• yield tolerance

multiple genes

quantitative trait

MaterialsNumber Variety/line Number Variety/line Number Variety/line

1 HC 540 13 95-38-34 25 972-B5

2 NING 68331 14 96-12-10 26 AC Norboro

3 Wong Ju 15 AC Hartland 27 AW 492

4 H 281 16 AW 373 28 AW 491

5 Sumai-3 17 QW 625.25 29 Celtic

6 AW 495 18 QW 628.5 30 Pacific

7 29AA28 19 AW 466 31 Glenlea

8 AC Drummond 20 AC Helena 32 AW 493

9 Nyu Bay 21 Belvedere 33 AW 498

10 AW 478 22 Grandin 34 AC Walton

11 AW 499 23 Max 35 Roblin

12 95-51-10 24 972-B1   

Field experiments for evaluation of FHB resistance

_______1____2____3____4____5____6____7____8__

row1 row2 row3 row4 row5 ……

Fusarium head blight index

FHB Index

＝ severity incidence X severity in infected heads

調查分為兩大項：

第一項為有症狀的穗佔全體之比例。

第二項為被感染穗之嚴重程度。

Severity in infected heads

Adjusted FHB indexRoblin FHB index =100

Genetic diversity

I

II

a

b

c

d

Principal coordinates analysis

I

II

Principal coordinates analysis

China

Markers associated with FHB resistance

FHB index <30.0 FHB index >60.0

HC540, MING68331, Wong Ju, H281, Sumai-3, A

W495, 29AA28, AC Drummond,

Nyu Bay, AW499,

972-B1, 972-B5, AC Norboro, Pacific, Glenlea, AC Walton, Robl, 9

5-51-10, 95-38-34, 96-12-10,

2 test Three markers, H191000, F2500 and B12400,

were associated with low FHB index.

Breeding for FHB resistanceBreeding for FHB resistance is difficult

• poor agronomic traits associated with FHB resistance

• the inheritance is conditioned by multiple genes • screening for FHB resistance is environmentally biased

Markers associated with FHB resistance

ProspectProspect • Information about genetic relationships among

accessions within species has been shown to be useful for the selection of parents for hybridization.

• This study detected three RAPD markers significantly associated with FHB resistance; these will be useful for marker-assistant breeding.

Thank You