專題討論 題目 : RAPD polymorphisms in spring wheat cultivars and lines with different level of...
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Transcript of 專題討論 題目 : RAPD polymorphisms in spring wheat cultivars and lines with different level of...
專題討論題目 : RAPD polymorphisms in spring wheat cultiv
ars and lines with different level of Fusarium resistance
作者 : G. Sun, M. Bond, H. Nass, R. Martin and Z. Dong
出處 : Theor Appl Genet (2002)
指導教授 : 王裕文老師
報告學生 : 蔡依真
Fusarium spp. 鐮胞菌Fusarium head blight (FHB)
小麥 大麥
Fusarium head blight
FHB resistance
• resistance to primary infection
• resistance to subsequent colonization after infection
• resistance expressed in the developing kernel
• reduced accumulation of mycotoxins
• yield tolerance
multiple genes
quantitative trait
MaterialsNumber Variety/line Number Variety/line Number Variety/line
1 HC 540 13 95-38-34 25 972-B5
2 NING 68331 14 96-12-10 26 AC Norboro
3 Wong Ju 15 AC Hartland 27 AW 492
4 H 281 16 AW 373 28 AW 491
5 Sumai-3 17 QW 625.25 29 Celtic
6 AW 495 18 QW 628.5 30 Pacific
7 29AA28 19 AW 466 31 Glenlea
8 AC Drummond 20 AC Helena 32 AW 493
9 Nyu Bay 21 Belvedere 33 AW 498
10 AW 478 22 Grandin 34 AC Walton
11 AW 499 23 Max 35 Roblin
12 95-51-10 24 972-B1
Field experiments for evaluation of FHB resistance
_______1____2____3____4____5____6____7____8__
row1 row2 row3 row4 row5 ……
Fusarium head blight index
FHB Index
= severity incidence X severity in infected heads
調查分為兩大項:
第一項為有症狀的穗佔全體之比例。
第二項為被感染穗之嚴重程度。
Severity in infected heads
Adjusted FHB indexRoblin FHB index =100
Genetic diversity
I
II
a
b
c
d
Principal coordinates analysis
I
II
Principal coordinates analysis
China
Markers associated with FHB resistance
FHB index <30.0 FHB index >60.0
HC540, MING68331, Wong Ju, H281, Sumai-3, A
W495, 29AA28, AC Drummond,
Nyu Bay, AW499,
972-B1, 972-B5, AC Norboro, Pacific, Glenlea, AC Walton, Robl, 9
5-51-10, 95-38-34, 96-12-10,
2 test Three markers, H191000, F2500 and B12400,
were associated with low FHB index.
Breeding for FHB resistanceBreeding for FHB resistance is difficult
• poor agronomic traits associated with FHB resistance
• the inheritance is conditioned by multiple genes • screening for FHB resistance is environmentally biased
Markers associated with FHB resistance
ProspectProspect • Information about genetic relationships among
accessions within species has been shown to be useful for the selection of parents for hybridization.
• This study detected three RAPD markers significantly associated with FHB resistance; these will be useful for marker-assistant breeding.
Thank You