臨床生化（（ⅡⅡ）） Instrumentational Analysis

賴滄海 教授10-23-2007

Individual Steps In the Analytical Processes

• Specimen acquisition

• Specimen identification

• Specimen delivery to laboratory

• Specimen preparation

• Specimen loading and aspiration

• On-analyzer specimen delivery

• Ka = 【 Hp -Ab 】 【 Hp 】【 Ab 】

Ka = affinity constant

免疫檢驗的分類• 方式 Homogeneous Heterogeneous

• 原理 Competitive Sandwich

• 標誌物質 RIA FIA EIA CLIA

Examples of IAs • Radio immunoassay (RIA)• Particle –enhanced turbidimetric inhibition IA (PETINIA)• Enzyme-linked Immunosorbent Assays (ELISA)• Enzyme multiplied Immunoassay Technique (EMIT)• Cloned enzyme donor IA (CEDIA)• Microparticle capture enzyme IA (M EIA)• Solid phase Fluorescence IA (SPFIA)• Particle concentration Fluorescence IA (PCFIA)• Fluorescence excitation transfer IA (FETI)• Substrate-level Fluorescence IA (FPIA)• Dissociation-enhanced lanthanide fluoro imnurco assay

(DELFIA)