UNITN-Trento
Solar-pMFC
PR
PR
araC-pBAD
araC-pBAD
Proteorhodopsin
Proteorhodopsin
LIGHT DARK
More H+ pumping outside!
0
5
10
15
20
BBa_K731201 BBa_K1604010
Dark DarkLight Light
araC-pBAD Proteorhodopsin
ADP/ATP ratioPR increases ATP production! AD
P/AT
P ra
tio
β-carotene gets used up!
β-carotene β-carotene+blh
2+
Abs
orba
nce
(450
nm)
1,0
2,0
3,0
4,0
LB LB LB + NATerrific Broth
Terrific Broth
pncB BBa_K1604031 araC-pBAD BBa_K731201
pncB increases NAD+ concentration!N
AD
+/N
AD
H
LB + Nicotin acid
NAD
+ total
pncB produces more NAD+ without O2!
BBa_K731201 BBa_K1604010
Proteorhodopsin enhances MFC performance power curve
Pow
er (μ
W)
0
3,5
7
10,5
14
Current (µA)
0,01 0,1 1 10 100 1000
BBa_K731201BBa_K1604010
Volta
ge (V
)
0
0,15
0,3
0,45
0,6
Current (µA)
0,01 0,1 1 10 100 1000
BBa_K731201 BBa_K1604010araC-pBAD Proteorhodopsin
PR enhances MFC performance
ATPATPATP
pncB enhances MFC performance
Vara(V) VaraNR(V) VaraMB(V) VpncB(V) VpncBNR(V) VpncBMB(V) Iara(uA) IaraNR(uA) IaraMB(uA) IpncB(uA) IpncBNR(uA) IpncBMB(uA) Para(uA) ParaNR(uA) ParaMB(uA) IpncB(uA) PpncBNR(uA)PpncBMB(uA)
10000000 100000 0,53 0,557 0,612 0,512 0,567 0,534 0,00265 0,053 0,0557 0,0612 0,0512 0,0567 0,0534 0,000795 0,02809 585375,471698113672430,8797127470,0262144 627908,203125502920,634920635
50000 500 0,415 0,537 0,592 0,484 0,544 0,524 0,002075 8,3 10,74 11,84 9,68 10,88 10,48 0,1245 3,4445 694865,060240964652633,1471135944,68512 611438,016528926504735,294117647
25000 250 0,345 0,502 0,562 0,455 0,532 0,509 0,001725 13,8 20,08 22,48 18,2 21,28 20,36 0,207 4,761 730446,376811594629171,314741036 8,281 622030,769230769486994,360902256
20000 200 0,318 0,497 0,541 0,437 0,511 0,497 0,00159 15,9 24,85 27,05 21,85 25,55 24,85 0,2385 5,0562 776757,86163522588895,3722334 9,54845 597530,892448512483383,561643836
15000 150 0,278 0,468 0,519 0,421 0,511 0,48 0,00139 18,5333333333333 31,2 34,6 28,066666666666734,0666666666667 32 0,278 5,15226666666667787856,115107914575557,69230769211,8160666666667620239,904988124450880,626223092
10000 100 0,228 0,449 0,489 0,387 0,486 0,455 0,00114 22,8 44,9 48,9 38,7 48,6 45,5 0,342 5,1984 884214,912280702532563,47438752814,9769 610325,581395349425977,366255144
1000 10 0,056 0,185 0,258 0,127 0,244 0,223 0,00028 56 185 258 127 244 223 0,84 3,136 611160,714285714359805,40540540516,129 468787,401574803203807,37704918
500 5 0,025 0,024 0,025 0,049 0,057 0,07 0,000125 50 48 50 98 114 140 0,75 1,25 23040 26041,6666666667 4,802 66306,122448979685964,9122807018
Volta
ge (V
)
0
0,2
0,4
0,6
Current (uA)
0,01 0,1 1 10 100 1000
BBa_K731201 BBa_K1604031
Polarization curve
V
0
0,15
0,3
0,45
0,6
uA
0,01 0,1 1 10 100 1000
araNRPncbNR
Polarization curve
V
0
0,175
0,35
0,525
0,7
uA
0,01 0,1 1 10 100 1000
araMBPncbMB
Polarization curve
V
0
25000
50000
75000
100000
uA
0,01 0,1 1 10 100
araaraaraNR
Polarization curve
V
0
0,15
0,3
0,45
0,6
uA
0,01 0,1 1 10 100 1000
pncBpncBNRpncBMB
araC-pBAD pncB
NAD+NAD+NAD+
QUALCHE TITOLO?
NORMAL LAMP
LOW CONSUMPTIONLAMP
LED OUR SYSTEM
600 W 100 W 15 mW 200 uW
In the future Synthetic Biology and household consumption must meet.
Promoter strength ratio is consistent among techniques
Attributions
Support
Wet lab: Alessandro, Claudio, Elisa, Silvia, Riccardo, Veronica. Wiki design: Moreno. Wiki content: everybody. MFC: Alessandro and Riccardo. Interlab: Claudio and Elisa. HP: Elisa. Modeling: Kent 2015 iGEM team. Logo and graphics: Gianmarco. Poster: Veronica and Elisa.
HPLC: Graziano Guella. Electronic instruments and advice: technical staff of the Physics dept. FACS and qPCR: Mansy lab. Instructors: Cristina Del Bianco, Sheref Mansy, Martin Hanczyc, Olivier Jousson.
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