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HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIA

TITLE :BONE MARROW:

SAMPLE PREPARATION AND STORAGE VERSION NO. 1PROCEDURE NO. HUSM/HEMA-UPT/STM-BM1 VERSION DATE. 24.03.2011

Page 1 of 3 STANDARD TECHNICAL MANUAL

APPROVED BY:

...ASSOC PROF DR ROSLINE HASSAN

HEAD OF HAEMATOLOGY DEPARTMENT

CONTROLLED COPY NO : 3

REGISTERED HOLDER

HAEMATOLOGY LABORATORY

RECORD OF REVIEW/AMMENDMENT

DATEVERSION

NO. DETAIL OF AMMENDMENT BY

8/3/2019 Husmhema Upt Stm Bm1

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HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIA

TITLE :BONE MARROW:

SAMPLE PREPARATION AND STORAGE VERSION NO. 1PROCEDURE NO. HUSM/HEMA-UPT/STM-BM1 VERSION DATE. 24.03.2011

Page 2 of 3 STANDARD TECHNICAL MANUAL

PREPARED BY : ANG CHENG YONG

DESIGNATION : TRAINING OFFICER / SCIENTIFIC OFFICER

CHECKED BY : DR SHAFINI MOHAMED YUSOFF

DESIGNATION : HAEMATOLOGIST

AUTHORISED BY : ASSOC PROF DR ROSLINE HASSAN

DESIGNATION : HAEMATOLOGIST/LAB DIRECTOR

1. OBJECTIVE

To prepare the smears for staining purpose.

2. METHOD

Bone Marrow spreading

3. PRINCIPLE

The smear should have head and trail

4. REQUIREMENTS

4.1 EQUIPMENT

4.1.1 Glass slides

4.1.2 Spreader

4.1.3 Slide holder

4.1.4 Gauze

4.1.5 Petri dish

4.2 SPECIMEN

4.2.1 Aspirated bone marrow4.2.2 Imprint

5 PROCEDURE

NO. ACTIVITY RESPONSIBILITY

5.1 From bone marrow aspiration

5.1.1 Obtain aspirated marrow from the medical personnelimmediately and expel it into petri dish

MLT/SO

5.1.2 Rotate the petri dish to look for the marrow fragment MLT/SO

5.1.3 Gently touches the marrow fragment onto a spreader then transferit to the glass slide

MLT/SO

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HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIA

TITLE :BONE MARROW:

SAMPLE PREPARATION AND STORAGE VERSION NO. 1PROCEDURE NO. HUSM/HEMA-UPT/STM-BM1 VERSION DATE. 24.03.2011

Page 3 of 3 STANDARD TECHNICAL MANUAL

5.1.4 Drag the fragment and leave a trail of cells behind the spreader MLT/SO

5.1.5 Repeat the step 5.1.3 to 5.1.4 to get at least 7 slides for newcases and at least 3 slides for old cases MLT/SO

5.1.6 Label the slides with

i) Patient R/N and

ii) Date of sample collection

MLT/SO

5.1.7 Allow the slides to dry in the air before stain MLT/SO

5.2 From imprint

5.2.1 Obtain bone biopsy from the medical personnel and placed it inthe middle of one glass slide,

MLT/SO5.2.2 Placed a second slide on top of the first slides and rolled it

forward and backward gently for few times

MLT/SO5.2.3 Repeated step 5.2.2 for few time to get at least 2 slides MLT/SO5.2.4 Label the slides with

iii) Patient R/N and

iv) Date of sample collection

MLT/SO

5.2.5 Allow the slides to dry in the air before stain MLT/SO

5.3 For storage5.3.1 Wrap tightly the additional air-dried unfixed and unstained

smear with in aluminum foil

MLT/SO5.3.2 Label it with

i) Patients name

ii) Patients R/N

iii) Date of sample collection

MLT/SO

5.3.3 Storage it at 4oC-10

oC for future use purposes MLT/SO

6 REFERENCES6.1 S. M. Lewis, B. J. Bain & I. Bates. (2006) Dacie and Lewis Practical Haematology, 10

th

edition. Churchill Livingstone.

6.2 S.H.Lee et al. (2008) ICSH guidelines for the standardization of bone marrow specimens

and reports. International Journal of Laboratory Hematology 30, 349-364.

End of Document