H 2 O 2 (M)02000 0 0 Soluble / InsolubleSol Insol Sol Insol
Total protein7.19.02.77.018.517.57.26.0 Insoluble adjusted by
soluble protein 0.40.86.54.40.40.3 % change+107.1-12.7 SpeciesHouse
mouseChinchilla MLS (years)4.017.2 Supplemental Figure 1:
Representative blot of detergent insoluble protein
Slide 2
H 2 O 2 (M)01000 0 0 Disulphide bonds9.915.512.911.6
10.017.516.1 Total protein2.42.51.61.21.82.35.35.0 Disulphide
(adjusted to total protein) 4.16.38.29.66.54.43.33.2 %
change+55.3+16.6-32.7-1.7 SpeciesShrewGerbilFox SquirrelChinchilla
MLS (years)2.26.316.017.2 Total protein Disulphide bonds
Supplemental Figure 2: Representative blot of disulphide bond
formation Unbound Region of label quantification
Slide 3
Supplemental Figure 3: (A) scatterplot of maximum lifespan
against body mass (log 10 scale) in 52 species of mammals. (B)
Scatterplot of change in protein carbonyl content following
exposure to 100 M H 2 O 2 body mass (log 10 scale) in 52 species of
mammals.
Slide 4
Supplemental Figure 4: Mammalian phylogeny, values at nodes are
millions of years since divergence
Slide 5
Supplemental Figure 5: Reference list for construction of
phylogeny
Slide 6
A C B Supplemental Figure 6: Scatterplot of % change in protein
carbonyl content following exposure to 5 mM paraquat. (A) Data for
16 species of rodents. (B) Data for 19 species of primates. (C)
Data for 14 species of Lauriasiatheria.. Cells from humans are
shown in the scatterplot (with the "H" symbol) but not used in the
statistical analysis. Values are adjusted by total protein content
in each sample. Error bars, if present, represent SEM of 2-3 cell
lines derived from independent animals.
Slide 7
Supplemental Figure 7: Scatterplot of % change in protein
carbonyl content following exposure to 5mM paraquat plotted against
body mass (log 10 scale) in 52 species of mammals.
Slide 8
Shrew (MLS 2.2) Yuma myotis (MLS 8.8) Fringed myotis (MLS 18.3)
Big brown bat (MLS 19) Long-eared myotis (MLS 22) Little brown bat
(MLS 34) Wild sheep (MLS 22.8) Deer (MLS 23) Desert lynx (MLS 20.3)
Common raccoon (MLS 21) Cougar (MLS 23.8) Domestic dog (MLS 24) A B
C Supplemental Figure 8: (A) Representative blot of baseline
protein carbonyl content in (A) 15 species of rodent. (B) 14
species of primate (C) 12 species of Laurasiatheria. Hamster (MLS
3.9) House mouse (MLS 4.0) Cotton rat (MLS 5.2) Gerbil (MLS 6.3)
Nile kusu (MLS 6.7) Cactus mouse (MLS 7.3) White footed mouse (MLS
7.9) Deer mouse (MLS 8.3) Chipmunk (MLS 9.5) Degu (MLS 14) Fox
squirrel (MLS 16) Chinchilla (MLS 17.2) Porcupine (MLS 18) Beaver
(MLS 23.4) Grey Squirrel (MLS 23.6) Red-bellied lemur (MLS 20)
LHoests monkey (MLS 24.1) Olive Baboon (MLS 27.5) Squirrel monkey
(MLS 30.2) Vervet (MLS 30.8) Woolly monkey (MLS 32) Ruffed lemur
(MLS 36) Hamadryas (MLS 39) Baboon (MLS 55) Managabey (MLS 46)
Gibbon (MLS 55) Gorilla (MLS 55.4) Chimpanzee (MLS 59) Human (MLS
122.5) Total Protein Protein Carbonyl
Slide 9
Chinchilla (mls 17.2) Porcupine (mls 18) Beaver (mls 23.4)
Lemur (mls 20) Titi (mls 26.4) Macaque (mls 40) Rodents Primates
Total Protein Protein Carbonyl Supplemental Figure 9:
Representative blot of protein carbonyl content in 3 species of
rodents and 3 species of primate. Protein carbonyl content is
measured using an antibody against 2,4-Dinitrophenol (anti-DNP).
Total protein is measured using an india ink stain.
Slide 10
Rodent species Common Species NameLatin Species NameFamilyMLS
(yrs)Weight (g)Number of Donors Golden hamsterMesocricetus
auratusMuridae3.91051 Wild house mouseMus musculusMuridae420.53
Cotton ratSigmodon hispidusMuridae5.21851 GerbilMeriones
unguiculatusMuridae6.353.21 Nile kusuArvicanthus
niloticusMuridae6.71103 Cactus mousePeromyscus
eremicusCricetidae7.4251 White-footed mousePeromyscus
leucopsisCricetidae7.9231 Deer mousePeromyscus
maniculatusCricetidae8.320.51 ChipmunkTamias
striatusSciuridae9.5961 Red squirrelTamiasciurus
hudsonicusSciuridae9.82002 Guinea pigCavia porcellusCaviidae127282
DeguOctodon degusOctodontidae142351 Fox squirrelSciurus
nigerSciuridae168001 ChinchillaChinchilla
lanigeraChinchillidae17.2642.53 North American PorcupineErethizon
dorsatumErethizontidae1886003 American BeaverCastor
canadensisCastoridae23.4202503 Grey squirrelSciurus
carolinensisSciuridae25.65331 Supplemental Table 1: List of rodent
cell lines
Slide 11
Primate species Common Species NameLatin Species NameFamilyMLS
(yrs)Weight (g)Number of Donors Common marmosetCallithrix
jacchusCallitrichidae16.52551 Southern lesser bushbabyGalago
moholiGalagonidae16.61751 Coquerel's mouse lemurMizra
coquereliCheirogaleidae17.43111 Pygmy marmosetCallithrix
pygmaeaCallitrichidae18.61241 Red-bellied lemurEulemur
rubriventerLemuridae2017651 Red tamarinSaguinus
midasCallitrichidae20.55011 L'Hoest's monkeyCercopithecus
lhoestiCercopithecidae24.147001 Red titiCallicebus
cupreusPitheciidae26.411201 Olive baboonPapio
anubisCercopithecidae27.5240001 Common squirrel monkeySaimiri
sciureusCebidae30.29251 VervetChlorocebus
aethiopsCercopithecidae30.856204 Common woolly monkeyLagothrix
lagotrichaCebidae3276501 Black & white ruffed lemurVarecia
variegataLemuridae3636701 Hamadryas baboonPapio
hamadryasCercopithecidae37.5180002 Rhesus macaqueMacaca
mulattaCercopithecidae4082352 Red-capped mangabeyCercocebus
torquatusCercopithecidae4694931 BonoboPan paniscusHominidae55399251
GorillaGorilla gorillaHominidae55.41398422 White-handed
gibbonHylobatus larHylobatidae5668101 OrangutanPongo
pygmaeusHominidae59644751 ChimpanzeePan
troglodytesHominidae59.4449842 HumanHomo
sapiensHominidae122.5620352 Supplemental Table 2: List of primate
cell lines
Slide 12
Laurasiatheria species Common Species NameLatin Species
NameOrderMLS (yrs)Weight (g)Number of Donors Northern Short-tailed
ShrewBlarina brevicaudaSoricomorpha2.221.63 Yuma MyotisMyotis
yumanensisChiroptera8.86.251 Fringed MyotisMyotis
thysanodesChiroptera18.361 Desert lynxCaracal
caracalCarnivora20.3160001 Big brown batMyotis
lucifugusChiroptera19232 RaccoonProcyon lotorCarnivora2160002
Western Long-eared MyotisMyotis evotisChiroptera227.41 Wild
SheepOvis ariesArtiodactyla22.8800002 White-tailed deerOdocoileus
virginianusArtiodactyla23870001 CougarPuma
concolorCarnivora23.4630001 Domestic dogCanis
familiarisCarnivora24400001 OcelotLeopardus
pardalisCarnivora28.2880001 BobcatLynx rufusCarnivora32.3940002
Little brown batEptesicus fuscusChiroptera34103 Supplemental Table
3: List of Laurasiatheria cell lines
Slide 13
Bird species Common Species NameLatin Species NameOrderMLS
(yrs)Weight (g)Number of Donors Yellow-rump warblerDendroica
coronataPasseriformes6.912 1 Horned larkEremophila
alpestrisPasseriformes833.5 1 Carolina wrenThryothorus
ludovicianusPasseriformes9.217.5 1 KilldeerCharadrius
vociferusCharadriiformes10.988 1 House finchCarpodacus
mexicanusPasseriformes11.621.4 1 Barn swallowHirundo
rusticaPasseriformes1618.3 1 American robinTurdus
migratoriusPasseriformes1775.5 1 Gray catbirdDumetella
carolinensisPasseriformes17.934.5 1 GadwallAnas
streperaAnseriformes22.3791 1 Wood duckAix
sponsaAnseriformes22.5452.8 1 PheasantPhasianus
colchicusGalliformes271095 1 MallardAnas
platyrhynchosAnseriformes29.11,048 1 Mourning doveZenaida
macrouraColumbiformes31.3119 1 Rock doveColumba
liviaColumbiformes35358.7 1 OstrichStruthio
camelusStruthioniformes50111,000 1 Supplemental Table 4: List of
bird cell lines
Slide 14
Common Species NameLatin Species NameMLS (yrs)Corriell Cell
Line Name Southern lesser bushbabyGalago moholi16.6PR00519
Coquerel's mouse lemurMizra coquereli17.4PR01044 Pygmy
marmosetCallithrix pygmaea18.6PR00839 Red-bellied lemurEulemur
rubriventer20PR00273 Red tamarinSaguinus midas20.5PR00550 Red
titiCallicebus cupreus26.4PR00793 Olive baboonPapio
anubis27.5PR00033 Common squirrel monkeySaimiri sciureus30.2PR00612
Common woolly monkeyLagothrix lagotricha32AG05356 Black & White
ruffed lemurVarecia variegata36PR00293 Red-capped
mangabeyCercocebus torquatus46PR00485 BonoboPan paniscus55PR00235
GorillaGorilla gorilla55.4PR00055PR00278 White-handed
gibbonHylobatus lar56PR001131 OrangutanPongo pygmaeus59PR01107
ChimpanzeePan troglodytes59.4PR00175PR00115 HumanHomo
sapiens122.5GM02937GM02912 Supplemental Table 5: List of cell lines
obtained from Corriell cell repository