VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome...
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Erwin R. Schmidt Institut für Molekulargenetik Vorlesung #4 07. 05. 2013 VL Einführung in die Gentechnologie
Transcript of VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome...
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Erwin R. Schmidt Institut für Molekulargenetik
Vorlesung #4 07. 05. 2013
VL Einführung in die Gentechnologie
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Substitutionsvektoren
Linker Arm „Stuffer“ Rechter Arm
Integrat
Ligation
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Sal Bam Eco Eco Bam Sal
Verdau mit Sal I + Eco
Sal Bam Eco Eco Bam Sal
Sal
Sal
Eco
Eco
Sal
Sal
Fällen mit Isopropanol
Eco
Eco
Sal
Sal
Integrat
Linker Arm Rechter Arm „Stuffer“
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Lambda Zap: Moderne Vektoren Mit Phagen und Plasmid- eigenschaften
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P-Elemente sind Transposons bei D. melanogaster, die eine Keimbahn-spezifisch aktive Transposase exprimieren