START-UP OF ANAMMOX PROCESS IN MBBR SYSTEMdosya.marmara.edu.tr/eng/enve/Lisans_Programi... · MBBR...
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Department of Environmental Engineering – The University of Marmara Istanbul, Turkey Burcu Şerit, Dilan Yakar, Ece Kain, Özge Ekiz and Rana Bekçe E-mail: [email protected], [email protected] , [email protected] , [email protected] , [email protected] A-) START-UP OF ANAMMOX MBBR WITH ACTIVATED SLUDGE B-) START-UP OF ANAMMOX MBBR WITH ENRICHED ANAMMOX Why Anammox Process? Anammox process is a revolution for sustainable wastewater treatment. Pollutants become a renewable energy source by this process. Anaerobic and autotrophic Anammox bacteria eliminate the external carbon addition and CO 2 emission. This process reduces the oxygen requirement and excess sludge treatment cost. Why MBBR? MBBR is a flexible solution for nitrogen removal processes. This is an attached growth system, polyethylene rings have a high surface area they can carry large biomass population. MBBR systems do not have sludge recirculation and providing long sludge age, thus they are efficient for processes including slow growing bacteria (eg. Anammox). Results & Discussions CONCLUSION • Inhibition of the heterotrophic bacteria was identified at feeding composition changes. They could not adapt easily to a new feed solution. • Heterotrophic attachment was observed at 49 th day. • The highest removal efficiency was observed at acetate and sewage wastewater feeding under continuous flow conditions. Beef extract and peptone solution was inhibited the heterotrophic bacteria. • Enriched Anammox granules were larger for attaching. Free cells were not attached in the study period. Next Step Anammox seeding will be occurred to MBBR for Anammox bacteria attachment over the heterotrophic layer. Seed Waste activated sludge : • Bursa Doğu WWTP’s RAS line. • WAS supplied from Ataköy WWTP. Materials & Methods Materials & Methods Results & Discussions Seed • Enriched Anammox seed was supplied from Bursa Doğu STP Mainstream Anammox Pilot Plant . • Anammox bacteria were in granular form (0.5-1cm) they were transformed to free cells with magnetic stirrer. • MBBR was designed as batch at first phase then continuous flow was provided in the reactor. • pH: 7.5 Temperature: Room Temperature Dissolved Oxygen: 6 mg/L Feed Reaction Settle Decant Duration(hour) 1 21 1 1 Time Interval 16.00-17.00 17.00-14.00 14.00-15.00 15.00-16.00 Feed Pump ON OFF OFF OFF Magnetic Stirrer OFF ON OFF OFF Air Pump OFF ON OFF OFF Decant Pump OFF OFF OFF ON START - UP OF ANAMMOX PROCESS IN MBBR SYSTEM 0 100 200 300 400 500 600 700 800 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 COD (mg/L) Time (day) Influent COD mg/L Effluent COD mg/L I II III IV V 33.2% 40.2% 27.6% 30.7% 31.7% 0 50 100 150 200 250 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 NH4-N, mg/L Time ( day) Effluent NH4-N, mg/L Influent NH4-N , mg/L II I IV V III 43.2% 78.8% 37.9% 60.7% 51.2% 0 10 20 30 40 50 60 70 80 90 0 5 10 15 20 25 30 35 40 45 NO 2 -N, mg/L Time (Day) Influent N02-N, mg/L Effluent NO2-N,… • Anammox bacteria are sensitive for ambient conditions. pH: 7-7.5 Temperature: 26 o C %95 Ar % 5 CO 2 purging. Phase I Phase II Phase III Phase IV Phase V Acetate & Ammonium feeding at batch mode Acetate & Ammonium feeeding at continuous mode Real sewage feeding at continuous mode Beef extract & Peptone feeeding at continuous mode Acetate & Ammonium feeding at continuous mode 26.3% removal of NO 2 -N 0 10 20 30 40 50 60 0 5 10 15 20 25 30 35 40 45 NH4-N, mg/L Time (Day) Influent NH4-N, mg/L Effluent NH4-N, mg/L 32.2% removal of NH 4 -N Aim of the Study Evaluating different start-up strategies to provide quick attachment on carriers for Anammox process in MBBR systems. • Anammox bacteria are slow growing organisms and they are not easily attach to hydrophobic carrier. Clean ring Material: Polyethylene Surface area: 650m 2 /m 3 Phase I 39 th day No attachment was observed. Phase II 53 rd day Thin film layer was started to produce. Phase III 62 nd day Attachment and treatment was observed. Phase IV 76 th day Inhibiton of the bacteria was occured. Phase V 102 nd day Attachment and treatment was increased. Phase V 124 th day Attachment and treatment was observed. Clean ring Material: Polyethylene Surface area: 650m 2 /m 3 28 th day No attachment was observed. 39 th day No attachment was observed. Clean ring Material: Polyethylene Surface area: 125 m 2 /m 3 39 th day No attachment was observed.
Transcript of START-UP OF ANAMMOX PROCESS IN MBBR SYSTEMdosya.marmara.edu.tr/eng/enve/Lisans_Programi... · MBBR...
Department of Environmental Engineering – The University of Marmara Istanbul, Turkey
Burcu Şerit, Dilan Yakar, Ece Kain, Özge Ekiz and Rana Bekçe E-mail: [email protected], [email protected], [email protected], [email protected], [email protected]
A-) START-UP OF ANAMMOX MBBR WITH
ACTIVATED SLUDGE
B-) START-UP OF ANAMMOX MBBR WITH
ENRICHED ANAMMOX
Why Anammox Process?Anammox process is a revolution for sustainable wastewater treatment.
Pollutants become a renewable energy source by this process.
Anaerobic and autotrophic Anammox bacteria eliminate the external carbon
addition and CO2 emission. This process reduces the oxygen requirement and
excess sludge treatment cost.
Why MBBR?MBBR is a flexible solution for nitrogen removal processes. This is an
attached growth system, polyethylene rings have a high surface area they
can carry large biomass population. MBBR systems do not have sludge
recirculation and providing long sludge age, thus they are efficient for
processes including slow growing bacteria (eg. Anammox).
Results & Discussions
CONCLUSION• Inhibition of the heterotrophic bacteria was identified at feeding
composition changes. They could not adapt easily to a new feed
solution.
• Heterotrophic attachment was observed at 49th day.
• The highest removal efficiency was observed at acetate and sewage
wastewater feeding under continuous flow conditions. Beef extract and
peptone solution was inhibited the heterotrophic bacteria.
• Enriched Anammox granules were larger for attaching. Free cells were
not attached in the study period.
Next StepAnammox seeding will be occurred to MBBR for Anammox bacteria attachment over the heterotrophic layer.
Seed Waste activated sludge :
• Bursa Doğu WWTP’s RAS line.
• WAS supplied from Ataköy
WWTP.
Materials & Methods Materials & Methods
Results & Discussions
Seed• Enriched Anammox seed was supplied from
Bursa Doğu STP Mainstream Anammox
Pilot Plant .
• Anammox bacteria were in granular form
(0.5-1cm) they were transformed to free
cells with magnetic stirrer.
• MBBR was designed as batch at first phase then continuous flow was provided in the reactor.
• pH: 7.5 Temperature: Room Temperature Dissolved Oxygen: 6 mg/L
Feed Reaction Settle Decant
Duration(hour) 1 21 1 1
Time Interval 16.00-17.00 17.00-14.00 14.00-15.00 15.00-16.00
Feed Pump ON OFF OFF OFF
Magnetic
Stirrer
OFF ON OFF OFF
Air Pump OFF ON OFF OFF
Decant Pump OFF OFF OFF ON
START-UP OF ANAMMOX PROCESS IN
MBBR SYSTEM
0
100
200
300
400
500
600
700
800
0 10 20 30 40 50 60 70 80 90 100 110 120 130 140
CO
D (
mg/L
)
Time (day)Influent COD mg/L Effluent COD mg/L
I II III IV V
33.2% 40.2%27.6% 30.7% 31.7%
0
50
100
150
200
250
0 10 20 30 40 50 60 70 80 90 100 110 120 130 140
NH
4-N
, m
g/L
Time ( day)
Effluent NH4-N, mg/L Influent NH4-N , mg/L
III IV VIII
43.2% 78.8% 37.9% 60.7%51.2%
0
10
20
30
40
50
60
70
80
90
0 5 10 15 20 25 30 35 40 45
NO
2-N
, m
g/L
Time (Day)Influent N02-N, mg/LEffluent NO2-N,…
• Anammox bacteria are sensitive for ambient conditions.
pH: 7-7.5 Temperature: 26oC %95 Ar % 5 CO2 purging.
Phase I Phase II Phase III Phase IV Phase V
Acetate &
Ammonium
feeding at batch
mode
Acetate &
Ammonium
feeeding at
continuous mode
Real sewage
feeding at
continuous mode
Beef extract &
Peptone feeeding
at continuous
mode
Acetate &
Ammonium
feeding at
continuous mode32.2% removal
of NH4-N
26.3% removal
of NO2-N
0
10
20
30
40
50
60
0 5 10 15 20 25 30 35 40 45
NH
4-N
, m
g/L
Time (Day) Influent NH4-N, mg/L
Effluent NH4-N, mg/L
32.2% removal
of NH4-N
Aim of the StudyEvaluating different start-up strategies to provide quick attachment on carriers for Anammox process in MBBR systems.
• Anammox bacteria are slow growing organisms and they are not easily attach to hydrophobic carrier.
Clean ring
Material:
Polyethylene
Surface area:
650m2/m3
Phase I
39th day
No attachment
was observed.
Phase II
53rd day
Thin film layer
was started to
produce.
Phase III
62nd day
Attachment and
treatment was
observed.
Phase IV
76th day
Inhibiton of the
bacteria was
occured.
Phase V
102nd day
Attachment
and treatment
was increased.
Phase V
124th day
Attachment and
treatment was
observed.
Clean ring
Material:
Polyethylene
Surface area:
650m2/m3
28th
day
No attachment
was observed.
39th
day
No attachment
was observed.
Clean ring
Material:
Polyethylene
Surface area:
125 m2/m3
39 th
day
No attachment
was observed.
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