?1:e{1ifroxrn{r1ur11dn{ il?J. fld + ariud 2 nrn1.lerru - #uernru 2537

m'r { rfi a ntuai'Lunt € Fr sJ ri'l rfioTo st#Porcine zona pellucida

or3fi r-rrrotni::'oriuo{ frnm"nf

vtU?OOUA

nr:n I rir tff er"Lu#oifi u nr ri fi dr uqi n :ii fi da 6rio rf, u rion Eir.:nTutrj urqiififiq

^d(ctl-tutJlJ 2'lra0 ?DlTtJflfrun:Til UUO:rnr.:noirru:rrluro n:rxnl:yirenun::il orriju* 1 w*A , , a *r -a! o 611 , t d u ed A -o 0J LTzu?1 tltuntl ou:rLrLto rr'r,ro ir-iurrvr moi.Lta lillrn nollril n n'[ri'dn rfr oci'a.:nr:ql s

-.o--o , a i; r !t r V - | X !uonolnuu.luuflflt.ttou.Jtru wqon::rrlnlurrrlolLrJ uruuntrrl{u rdt.:oonr:'l'r.3u1finu rrn:eirtdqirueiaudr.ra.r airuiinr:rir#q{:rfiir{riju#ooiolfirLirrrxaruiu

U

n-ntT.: urn:o'rriiuoia'r'ld:-rnrri.;rar"Lelciornn"rra.rtunr:#.:rnnnr:riiu#oro.:fi'n{ fi.r

rrri'jrf,oq fuorfini:vt-oL u-lnlrel )r nr rfi,ilFr u nr:ff urruiaaaflruuEiifrdfi oro rfi e zu n9qv a g

^d 6l , < <r Arirrrfrr.::r:Jyri.:rure.lirnsi6nr:lii'urffriiuerrrrri,:rrnil:;nrrfi1-r.r4- 1 )^ 3 . ^l ada. u v taveatua d a I n il il ulr I n 01 uar n n r :fl r.J nr ruo Lo 116o r n nr rir.: o'u il o q I u6 I'Loin nrr

^:-,-.^^---ylql]urnrrnelrirtfro Leu"ldi6nr:vrr.:nfi rYrn:uivrurfiu 6a nr:1d' antizona peilucida

antibody tr"l#iunr:nrnrrfio#qiun-,usfiq:run"*uu ifiaufrlruurfurfioornifrnr:erru9u9

^.-.-o ^aJ A ^oX --..tt..oj -jq - d I L dAA q e, d t qvt n,nituel'lDau r.:rruulitjuinnrtvt[fzuno ilnoonu ill6nrtLu';ru ifrueir"["i'siru'l:.ial uns:a q v d uG A -

a

fl at flr tllTn n ntJ&Jtil n n lo o n ul oo o.: nr:nrrru6otunr:triTrur rnnqfioir (zona pelucida) lunr:qrnrrrfio r3ruorn

q

^ J, q ,:_^.e^i^. ^V., ] f , o <t a a a,6- , &n?tilnqm?rlunr::Jfiauluu riusrouufr.lfr.:sirriJu6o nt:f]flrilTilto.tr,.,l-.lur1{ iryur,^..^^.3-v- ---?' ard a T e <tLylnauq'l n1l0lJ1J0.lflt:-lirululirto.: lryilr tnnnfroi'r astiJuoqtiuoi'ulolnr::Jfrauirunro'rriJuqionr:fl.:qirro,:o"'reiau q-.rfuurnfin1:yi.r01unn'[nnr:rfio:.Jfrniur::ra.jr.r

at c & Ta tTJ 5 ru n u lrur n l n n fr oir fr o : ar I r : n fl o.: ri'u n r :rJ fr aui'loi

' ntairtwatfrinn nruz#ntuwnuffisa{ xu1inil't#ulJauuriu {tniarauuriu 40002

? rlnf;nr*r&'qrunmri nr*:fiorunmuersn{ rnrinora'urouuriu o-.rur-o:rouuriil 40002

a9

90

Zona Pellucioa (ze) riJudula.:vat-4,

fl1:no1u[on1qu (gelatin) ry.]aUToU'lut, - (N ehit o.: d'qi 16 u.: nnoi'r uul tiiusr::Jrin ou

qJ

lnoInLJ:6u drfinrualuGtiju tissue-q

specific ttsililtilu species specific firarir

d rfl u specific receptor rilaiulv{ariJillua,a r& e t v ll r

flil56t1t1{ 6l il'tou unlta1i9{'11{t?1 UJT'Ilrd x, a ^!q?nuil'rrnRUfllan t1 rnqn'r:uilfl1151j1*

Tmir rn anfi oir :J*n ousT'r ulnnTnhj:du.U

Ad'3 5Uq ne ZP . ZP . ZP s{ ZP t:Ju

1' 2' 3 3

sperm receptor rafi'ndrirrurlfHfi qindu

nilnh rfiq (contraceptive vaccine)

n1:loligil Porcine zona pellucida

lqifi nr:ilTfl ulnr:tqriu il Porcine

zona pellucida a'rH:-utfrfl uuou6 tail

ranru?f uqrr#nnr:vr-rLjda vrhtvfT v sU, A A :Lor.: flT't.tr 0{T.l [u n n o qfi T 0u 9t til ua ilci'rurfin 1 ufrr6.:rfiubjuog zona pellucida

n1R follicle

n1Tlaiuil Porcine zona pellucida

(Henderson uaiflili, 1 988). uu,i r 6 lle Z <

u't r.l t1J s.t ttT ttlj.l t? tJ't 0 r n1 u u't [tlj.:evXAvA

un?aqtuotuoTau 6J oan uqo?u[nTo.]Xeauotilo uei'rtdr ice-cold saline (O.9Eo)

TaIl6lui{ thomoginize) riruorrrir u'[un ou

llu'ret 5o0 rroi ZOO 'lruIn:rrue: ,duXAAa

rah tfl r*rfr a ril ofr ilr ur olraqj o on u#r n:or

iiruqrdrururo 75 hTqr:rilq: rdo

rfiuciruto.:'lri #rtsirl ice-cold saline

KKU" Vet" J. Vol. + No.z July - December 1ee4

rrd'rriru Percoll gradient rn-.ta1nfi'u d1

tdd'ld'rur ir u n pe rco I I o a n lah 6'luii'lu*[qi'

zona 6s:; rrd:#rteiruil:;no:rttneidu roan yi''t zonaheat-solubilized d t6'n,ZO

aAqdr,1*'t11 t1^t0 Lu [oaluta.: zona pellucida

eglycoprotein a''lnil ilYt't zona

ultracentriluged tt#ri tn:r rvirar rJi lr nrfrAeadaffrAAoLilTOUO? U?! O[An LOT Lil nfl fl il',] fl11.{1 0.]

<VtduAo$,Qezona [nt L? Ll.tflR']?to9rtt14.: tilaul LIJ Lr

CIA Lil

AAd?fin1TilO

o f , ov A ueu'r [sl{'r tl^tflfls9l't ytttun Lo ttnt< lld 9 v

tnu ['] Luflnl?30qttil.iil1nsn1u tta')q,

tlflil9i?U Freund's complete adjuvant tuA , u qqlllva

ilTil10Ty1t141nU !U L9lilTil'lqr 1 ildnnq:

(r-l::lrcx 2000 zonae) dorriT nd'rrarilg n*r Iquvirrfu:Jrsil'rru 1-2 fr'rjqrf

rrsioslf Freund's incomplete adjuvant luAe&,u'-Y

n1Tilq nTr qtilnT.: ql 0 [!u analnnT.] ttTn3

, ll da e o a0 u''t.: lTn q 01Q0!q 0.:il1n1Taa

uYanrrd'usilAnua{aln 3 rfiruu:n 9 r6ou

AAAddquT0nlTqotilu.ttljil to u? [uluttTnl a{:3 ut

proestrus frorovir'[ai' 6'oo'.lnzuan1:r q e s u E u I4v

vl q n 0{}lU?'l t14 9{ nLJ 0.: n iln1:9l .lYl 0.: [91y,yn.: 3 ttilll1Jo.:nr:n:lgtu

n a"[n nr :fl a s fi]$nr :rJ fi ar+BAq

rfr o.:orn.Lunr:rJfr auG qiolfi nr:t at6 u & L Av

0unuT!14'r'1.: fl !:JTil nLTu L{u1 til n nTgl't- Z " ,i , r -t9ltuu141nfllnlTt! nuuttil n.: tflT.l fl:1.11J 0.1

v d a,a - d?153{1T6lsl1rry{nUFl'ldn5 il?J. ilyi 4 ouuYr 2 n:ngrelru - iuererr 2s3T 91

Iryr.ir ir^rnnfiai'r fio:arrur:nil0.:riunr:qt

ard Vl e v Ltnl31J0.lfrtilTu [o nlTnT;ou9l1u porcine

zona pellucida orvrrld'ir.:nrrair.:Joqvazona antibody lJ urJ'r 11t [u tn o

vimmunoprecipitation uuT1.[u0nt0.] zona

pe|Iucida fi zu n r or-.ru3on-oIrr.r qir rruu.rdAd a a <, V! A o q tflLilTilA:iltA! 0.1t1lunn Inilil't Lun]5

Ju,u-ililflufrno1Ju Lil [o

619/ 5l v drunZo0nlT[5 zona pellucida [Ua{Ot

n?'l,r Turner !!n:fln{: ( f OOZ) toi:ru.:ru

e.r fi I a { nr rtd'io 6un ru rir rfi olun I r lo'r ur uI

d vl ,o t o o J q/ ol 2nfl 0 LN:.Jnl:o.tyt0.ltno:i]uLun?liY--vt,La

il.t 7 a't ttn: Llyluzundl.ltnu.:

nrvsilu Bhatnager L[n:flo{: ( i OOZ)6)q

:'l U J lunlT tT deglycosyiated zona

peilucida tunr::l o.:funr:ilfr au81u

n::qir ri r^rriir'loizunvrn d'r uqifi zu ni!n?iln r :n'r J'r 1.r1 o.: i.: iri Il u o u -, r* n r : e ;r.r td

avj r Z - ovl , grioilnq Lil il't14un?J8nT.t'L?ilom.t T?lJli.1

" vt ,i011{?14 t"}l?1fin

Jones Lrniotru; (tsoz) Ttunrlr'd1't tJi{afloatu"tuta{ primary, secondary,

tertiary follicle Iou'[lriolvfrfionr:'j uu, - & a

til n uiluil n.t?t''1.:o nil u'tr? o.:i.t'[d e.:uuilq

ariunrlunr:"[ri' degtycosytated zonac

pellucida rfl u urouGiourdor^ieiurindu,u,

n3.Jn1tuo9,t0lil

#r Kirkpatrick !!a3nru3 (1992) 11u.t1u

nrrld' porcine zona pettuciOa (eZe) luvtal 9

nlTO8JnltilOrJ't?'t n1T[14 PZP OqCIonuq

...df(e3 il nouno9.lflil$rur 6[]il1Tnil0.:nunlT9

&euv,oi-.:yio.r'[oi'lrnnr'] 9oo/o usr arolruil3rur rxnr:n ff.: r o sTo :ro il0 o n.rtuflu'r.r o--l

Liu rrnrnil: ( t sa0) Ttu.tlunlTqo & u e, tL5 PZP 4 flTn ill,rnu 2-4 flilo-rl4 rrn?

u Y a !.q a Anriouslann;nlutgrOuyr 6- 1 0 lrd{aln

o Y qe Y - q on'']Toq nri ttrn Lu ilail 0.t nunlTo,tyl 0{ Luil1

86o/o L[nsr;q;iln fi ri'rriut-.1srrfi orifi ,: 7gcs

rd ou Io utrinrorr l fi orJn 6r o.r olnr:qrfae

::oltfl aT [ilu u:afl nl*ilsrtJanliu']f6s Sacco LLniFr{ur ( 1 9S7) rru.nunrr"ld'

zP 3 al n fl n:tufi.:'ir ar rr:n :J o.: ri'unr:q

Y - u' T9tJ?18,1 tO t9lU81A1\!nl:T!niUnlT

o o\4 ' u I,?'1 1,:''r 1.tfl 0,1 TJ LlJ t[d :'lr l_J i u n'] : fl T''].1 LlJ tto

- Y a o B ,cufl.3alnuil 10- 15 Lool-r ;.1lflnfl]illTno iu-

n tr rryiru',irfr'loiqr ilil nG

qfio Shivers lreinrut ( t Oat ) :ru.:'ru

nr:hi' pzP'Lusu-r'ir au-lfito"ir nr:99

n::ci'uo"r u PZP a;fl''lrirrnfl orri''unr:Y - uv ro.:yl0J [o Lo ua:wL immunoprecipitate

^ 4 da

formation tnq?u?lil-lo'tuu0nuan zonaA

pellucicla fi,:nro'jr immune complex fi^ A d n,v atnol u el 9Jn t:JlJo??''1.:niauqu.ta otnl3A u arr' u U 'uT0 nt::?tJ 911J 0,: fl tlJ:il nil L1

Mahi-Brown llnrnrut (1985)

nu'j1 adjuvant fl1ilt;nd?un:sei'u:sot-uq

rl6quA-YafLota 0: t14fl.t?t anasuu uinlTrufluoto [o u,u

. Vt ,, q ea e ^ iaalum 0r [iln0 tntnont:[ttltu:t'tilytaq ttqi

astli'loroof'[rin.:rvir Freund's rrns cp-g

20,96 1 adjuvant airunr:nJiuurfi uuntr99

nT;o! uft? u crude porcine zonaeq

92 KKU. Vet. J. Vol. I No.z July - Decembeltgg4

^EN,.lriavrifiu rriu nrrlri' Purified Zp3 alphaq

rata Zp3 beta-oG tun:sqi'rulou'[rjyi'rq ea ,l uvl,Lu t n el nl T til n u u $il a.t il't.l 0 0 1,\ u't T?1'1.: T.t L?

(Jone un;nil!, 1 992) uonalnd Keenan

Lrnrnrus (t OOI ) a-.lwu'jrn'rrrrunrrou6reuquaSJu,ooLfiuTflylt 1{1.[ A; Lilvl't Lfin1Tyl1.1'11.[

& Vl ,a , all r o q d elJ0.tT.J [1J r,r OilnO Lilttnst[1{;U'r 114 [T

Deglycosylated Zona pellucida rai o

zp3 a'[nnInI:J:6utfluuau6routunr:^ualqduauRqlnflunlJnltuo fi'l Lunl:tgr:uil?n{u

I

qrruni rfioluourn q orovir'loilo unr:#r r o :r s fr-n 6 uo r n r rl :J'[ q qf,ti'r r o rr r f

AAfUfi T 0 n1:LqT U il LO U 0',1 fl U tYt fl Ufiyt't.t1 t ut?ern::r lunr:uun DNA a'rn ltr.ir rnn

n8qi'r ttnio'rflu Vaccinia Virus tijunru;qt

q q eu a o a[un1: Lfi 1 Fl{iln iln'] 11.t9 9l.t t5u? 9lsil

Ia q i.lv 'q v

1rfl'ru11.{o Lilnuil txau tul_Jo0:Ju

nlTnririltfioTo uld Porcine ZonaI

Pellucida riutijuif;drJn oon-u virtoiir ur 4 w' Vl ' v d v anl LTA-] U Lil fl ,i rLA! fl 01 fl',] tJ',];nn nu il''lil 0nq., g

vlrd ! - ^e v a t a

tq 0 n til 0T3q! niln Nnuflo 0.t n.t ttlt0stJ{q

:l ru u r t u I r { n r rfi ei o n'r r yf 't .r'r ill o .: :-.: trirra:::qi':taoillu rfi o.:ornu-.:o u'1u

9l

Tr fi 'i 1.1 n 1 :6 n rsr n r :1 {"8r l{t n 1 :d'l'lilld'fqAovd

nl q11 !U0U't fl qa3 il nlTul? nSilfl ilnt tugl

Iq u Porcine Zona Pellucida trtri'niu

adr.:rtniunru

pellucidae (cPZP) n-upurified zona lrac-

tion

(peze) ilui'r n.rrnrreiuei?u cPZP a!{v ll cLnrun [otoo5fl.]uns:J1una1.: ttdsol0 Lno

.il

a <r v.l An?lil iloilnqi I 0.:?{TaunlTLljl{do tunr{u

fitorrqois.r ei'runr:n:;{uoiru pPZP o:

lra*lq ror oi::o-u:Jrun nr.:'].rra un'r:tiJ us&

floilnqt lqun.J CPZP un; pPZP fl'ril]:na Uv

tJ0.:nun1T9t6lilqq t9t

riodzaon,r:l# Isr.irOvdtr,[6{A')

rnaaf Eir

t ) fi onrurJnaqn-uun!fl;q?n. aa q ev a o l! w'

2 ) ?5',n']r 114?nTuYn [9t.]'ru

s) rirlfdru'[r.ia.:

+) fi nrrrudr til'rrei arruufi::n-ufq

fUrd,daAtq u tilil9{nq 0fl::l11 u'r01.{ 6l

5) nrrr:n n d''ururfi p n'[ai'6n rfr o3,t

Iq0{nt;

,t A O !f/l , ^ IuatGluzaf fl1Tt5 tsl{1 rilnafio1

$e a Ur, a

01aytuiln11.:tn u.: Lo lu]J1.:nrileaiveaa

zunll1.tLfi u.:Yt Ttu [o n 0 il un:iln?un1To uU ' o q e p !l ,

Yt'l.l'l u? a.: T.t LlJ 1,1't Lvt 1 u ? unl T fl :'1.1 Lll

fi qiln6 siruru'ld nn unrrseiilrosTorrxu

n9nnt u da v a

0u'1.: [Tnq 10tfiuo.]na1? 01a

ruriltlai'lq unr:ld'uauG reufi fi nr:yirtvi

?1:fl1rfi'o?ulrnsc{1aor{ N". fld + ariud 2 nrnglrrrJ - #r.lernru 2sgl

Rxllind 1eilnu1rr. 2537. nr:virrariu#o{ Tr,:firv{lrariyrur#ur::ilernoi nr.lrlrlte

ZqS uthAitken, R.J. , Paterson, M. and Koothan, P.T. 1993. contraceptive vaccines. Br. Med.

Bull. 49 (1) : BB-99

Bhatnagar, P. , Gupta , S.K. and Sehgal, S. 1 992 . lmmunogenicity of deglycosytated

zona Pellucida antigens and their inhibitory effects on fertility in rabbits.

lnt. J. Fertil. 37 (1) : 53-63

D'Occhio,M.J. 1 993. lmmunological Suppression of reproductive Functions in male

and female mammals. Animal reproductive Science. 33 : 345-372Gupta, S.K., Bagavant, H., Chadha, K. , Gupta , M., Yurewicz, E.C. and Sacco, A.G.

'1993. Mapping of lmmunogenic Domains on Porcine Zona - pellucida

3 - Alpha - Glycoproteins and Beta - Glycoproteins by Murine

Monoclonal - Antibodies. American journal of reproductive lmmunology.

30 : 95-100

Henderson, c.J., Hulme, M.J. and Ailken, R.J. 1988. contraceptive potential of

antibodies to the zona pellucida. J. Reprod. Fert. 83 :325-343Jones, G,R., Sacco, A.G., Subramanian, M.G., Kruger, M., Zhang,S., yurewicz, E.C.

and Moghissi K.S. 1992 Histology of ovaries of female rabbits

immunized with deglycosylated Zona pellucida macromolecules of pigs.

J. Reprod. Fertile. 95(2) : 51 3-25Keenan, J.A., sacco, A.G., subramanian, M.G., Kruger, M., yurewicz, E.c. and Mogh,

K.s. 1991. Endocrine response in Rabbits lmmunized with Native versus

Deglycosylated Porcine zona pellucida antigens. Biology of Reproduction.

44:150-156Kirkpatrick, J.F., Liu, l.M.K., Turner, J.w., Navgle, R.and Keiper, R. 1992. Long-term

effects of porcine zonae pellucidae immunocontraception on ovarian

function in feral horses (Equus caballus ). J. Reprod. Fert. 94 : 437-444Lir,l.K.M., Bernoco, M. and Feldman ,M. l gBg. contraception in mares

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93

94 KKU. Vet" J. Vol. 4 No.2 July * December 1994

Mahibrown , C.A., Yanagimachi, R., Hoffman, J.C. and Huang, T.T.F. '1985 . Fertility

control in the bitch by Active immunization with porcine zonae

pellucidae : Use of different Adjuvants and Patterns of Estradiol and

Progesterone Levels in Estrus Cycles. Biology ol Reproduction. 32 :

7 61 *772

Mahibrown, C.A., Mcguinness, R.P. and Moran, F. 1992. The cellular immune -

response to immunization with Zona Pellucida Antigens. Journal of

Reproductive lmmunology 21(1) : 29-46

Sacco, A.G., Yurewicz, E.C., Subramanian,M.G. and Demayo, F.J. 1981. Zona

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Potential of Antiserum to a Purified Pig Zona Antigen (pp=a). Biology of

Reproduction. 25 : 997-1 008

Sacco,A.G", Pierce, D.L., Subramanian, M.G. , Yurewicz, E.C. and Dukelow , W.R. 1987.

Ovaries Remaim Functional in Squirrel Monkeys (Saimiri sciureus)

lmmunized with Porcine Zona Pellucida 55,000 Macromolecule. Biology of

Reproduction. 36 : 481-490

Shivers, C.A., Sieg, P.M. and kitchen, H. 1981. Pregnancy Prevention in the dog:

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Wassarman, P.M. 1994. Gamete interactions during mammalian fertil ization.

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Characterization of a Purified Pig Zona Antigen (ppr") from porcine

oocytes. Biology of Reproduction. 29 : 51 1-523