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Blood Sampling

Draw 8 cc of blood Draw 13.5 cc ( 15cc Kit ) of blood18 cc ( 20cc Kit ) of blood

27 cc (30cc Kit ) of blood

By sampling the 18cc blood PRO PRP KIT can extract the needed PRP volume but

Regen kit have to use 2 tubes to extract PRP.

Regen PRP

PRP PRP

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Move Drawn Blood into the PRP KIT

Regen kit, containing Gel, is a kind ofvacuum blood collection tube, does not

move blood into another container.

Move drawn blood into the PRP kit.

Gel material injected into human body may possibly cause allergy, foreign body

reactions, and blockages in blood vessels.

Regen PRP PRO PRP

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Centrifugation

centrifugationSpeed : 1500G (3100 ~3400 RPM)

Time : 9~15 min

CentrifugationSpeed : 1050G (2800 RPM)

Time : 5 min

Regenlab recommends centrifugation at 3400rpm/5min, it may be a condition

to cause more damages. PRO PRP Centrifuge start slowly and stop slowly

In order to get fine PRP without damages

Regen PRP

PRO PRP

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Regen kit, containing gel in

tube, separates serum and

RBC layer like the bloodcollection tube.

After centrifugation, PRO PRP separates

PPP/PRP/Buffy Coat/RBC layer, and you can watch

the movement of Buffy coat layer with the naked eyewith parallel Layer which can extract fine PRP

*Regen kit: Each layer is not separated completely, so you can find some RBCs in

gel, which means Buffy coat layer is not completely separated and some remains

in gel and RBC layer, causing loss of platelets. Slope layer dont make to Extract

fine PRP

Regen PRP PRO PRP

After complete Concentration

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Analysis

Regen PRP PRO PRP

Regen have insisted that A is PRP but A is not a concentrated platelet but Serum .

Serums quantity is about 3.5~4cc . If insist all of 3.5~4cc PRP , its PRP density might be low.

If saying Regens insist for our Kit, PRO PRP Kit can say to get 10~12cc (20cc Kit ) PRP

including BuffyCoat on the aspect of Regen . But PRO PRP is 2cc high density PRP including

BuffyCoat . If you want to increase qty of PRP , you can extract more PRP by including PPP

Layer more.

A

A

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Reference

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Reference

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Extraction Step

Regen PRP PRO PRP

Withdraw 0.9cc ~ 4cc Extraction of PRO PPPMinimize PRP loss by complete PRP

extraction.

Regen kit uses the whole plasma layer after centrifugation, without separation

process of PPP/PRP/Buffy coat layers, so the concentration of platelets is low and

less effective in treatment. And, the extraction using a needle may possibly causecontamination.

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Summary

Regen PRP PRO PRP

*PRO PRP Kit may adjust the concentration of PRP, by adjusting the volume of PPP, and also

adjust RBC inclusion volume and Buffy-coat layer at doctors opinion and discretion.

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Microscope Images of the extracted PRP

A and B show the images of platelets clustered and activated after centrifugation.

(These images are not the case of Regen PRP).

In these conditions, PRP has no proper effects after the PRP procedure.

-Growth Factors already released outside the body are less effective in the body.

This phenomenon is found in the case of high-speed centrifugation, long time orrepeated centrifugations.

* C is the image of platelets extracted by PRO PRPsystem, which shows each platelet in

unimpaired, stable(inactivated) conditions.

A B C

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PRP PRP Microscope images

Stable state

Activated

state

PRO PRP SYSTEM

For effective PRP treatment, platelets should be

kept In their original conditions.

(they should not be activated in the early stages,

but collected in unimpaired conditions).

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Conclusion

Regen PRO PRO

Centrifugation condition 3100 RPM/9min or 3400RPM/5min

3,300RPM/ 5min (15cc,20cc)

2,800RPM/ 5 min (30cc)

Possibility of

contamination while

extracting

Possible contamination by using a

needleLittle possibility by not using a needle

Gel Used Not used

Dangerousness of Gel

Gel material injected into human

body may possibly cause allergy,foreign body reactions, and

blockages in blood vessels.

Just use the difference of densitygradient (among, RBC, buffy-coat and

plasma) without Gel

Volume 8ccBlood 16.5cc + anticoagulant 3.5cc

( total 20cc)

Control of PRP

concentrationDifficult Easy to control

Extraction volume of PPP,

PRP

Difficult to get PRP and PPP

separately : PRP + PPP = about 4cc

Extract PRP and PPP separately

PRP + PPP = about 11cc

Extraction of high-

concentrated PRPDifficult (Fixed) Easy

Extraction of low-

concentrated PRPDifficult (Fixed) Easy

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PRO PRP KIT TEST RESULT(Tested by Green Cross ReferenceLab)

672%

773%696%

806%733% 708%

809%

608%

1009%

792%

0%

100%

200%

300%

400%

500%

600%

700%

800%

900%

1000%

1100%

-

500.000

1.000.000

1.500.000

2.000.000

2.500.000

3.000.000

A B C D E F G H I J Average

Base line Test 1 Test 2 Test 3 Concentration rate(Max)

PatientName Base line Test 1 Test 2 Test 3 Concentration rate (Max)

A 214,000 1,366,000 1,439,000 1,424,000 672%

B 215,000 1,397,000 1,662,000 1,462,000 773%C 283,000 1,815,000 1,970,000 1,721,000 696%

D 283,000 2,267,000 2,280,000 2,137,000 806%

E 304,000 2,113,000 2,229,000 1,925,000 733%

F 200,000 1,666,000 2,202,000 1,925,000 1101%

G 258,000 1,826,000 1,630,000 1,622,000 708%

H 193,000 1,324,000 1,290,000 1,561,000 809%

I 300,000 1,824,000 1,288,000 1,439,000 608%

J 143,000 1,231,000 1,254,000 1,443,000 1009%

Average 239,300 1,682,900 1,724,400 1,665,900 792%

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TEST REPORT

Patient A

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TEST REPORT

Patient B

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Comparison betweenPRO PRP KIT & other PRP KITS

200

.000

247.

000

247.

000

27

4.

000

26

6.

000

25

8.

000

2.

202.0

00

1.

899.

999

1.

899.

000

1.

907.

000

1.

776.

000

1.

522.

000

1101%

769% 769%696% 668%

590%

0%

200%

400%

600%

800%

1000%

1200%

0

500.000

1.000.000

1.500.000

2.000.000

2.500.000

3.000.000

YES PRP A B C D E

Base line PRP count Concetration rate

Company

Name Base line PRP count Concentration rate

YES PRP 200,000 2,202,000 1101%A 247,000 1,899,000 769%

B 274,000 1,907,000 696%

C 266,000 1,776,000 668%

D 258,000 1,522,000 590%

E 258,000 1,150,000 446%

(Tested by Green Cross Reference Lab)

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Microscope Verification Result

CertificateOf numbers of platelets using YES PRP kit

Examined by laboratory at Cell & Tech corp.

Old

young

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