Preparo de biblioteca para sequenciamento Exemplos dos ... · DNA Insert Rd2 SP Index Rdl SP Index...
Transcript of Preparo de biblioteca para sequenciamento Exemplos dos ... · DNA Insert Rd2 SP Index Rdl SP Index...
Introdução à Bioinformática – IQB001
Rafael Dias Mesquita [email protected]
Laboratório de Bioinformática
Departamento de Bioquímica
Preparo de biblioteca para sequenciamento Exemplos dos kits TruSeq e Nextera da Illumina
Preliminar: PCR
http://bioinfo.iq.ufrj.br/graduacao/links/08%20PCR.swf
Kit TruSeq Illumina
DNA double strands created based on FW and RV strands
Strand specificity
U U U U U U This strand is NOT used as template on PCR
DNA double strand created based only on FW strand
Strand specificity
U U U U U U This strand is NOT used as template on PCR
DNA double strand created based only on FW strand
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf
Kit Nextera Illumina
https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-dna-flex.html
Kit Nextera Illumina
https://www.youtube.com/watch?v=-kTcFZxP6kM
Resumo de vários kits de preparação da amostra