PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint...

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Radionica II dan

Transcript of PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint...

Page 1: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Radionica

II dan

Page 2: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

MICRO-OXYMAX

RESPIROMETAR

Branka Lončarević,

Istraživač pripravnik, Institut za hemiju, tehnologiju i metalurgiju

Univerziteta u Beogradu

22.11.2018.

Beograd

Page 3: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM
Page 4: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Micro-Oxymax respirometar, Columbus Instruments, Ohio

Page 5: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

PRINCIP RADA

INSTRUMENTA

Page 6: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

OSNOVNI DELOVI SISTEMA:

Boce za uzorke

„Condensing air drier“

Senzori

Napajanja

Pumpa za vazduh

2 termometra

Sistemska pumpa za uzorke

Kalibracioni gasovi

„Gas Sample drier“

„Expansion interface“

Page 7: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Kolone sa drajeritom (sušilo)

Creva koja povezuju kalibracione gasove i

sistemsku pumpu za uzorke

Creva koja povezuju kolone sa drajeritom i

„sample drier“

Gumice, inserti, čepovi za boce

0.2 μm filteri

„Sample drier connections“

Ostali konektori

OSTALI DELOVI:

Page 8: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

H2

NO2

H2S

O2

SPISAK SENZORA

CH4

CO

CO2 (0-1 %)

CO2 (0-1 %)

IZMENLJIVI SENZORI

CO2 (0-1 %) ↔ CO2 (0-10 %)

O2 ↔ NO2

Page 9: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

SENZORI

Elektrohemijske ćelije

H2, NO2 i H2S senzori

Page 10: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

IR senzori

CH4, CO i CO2 senzori

SENZORI

Page 11: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Paramagnetni senzor

O2

SENZORI

Page 12: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

„Closed Loop

Measurement

Method” – Metoda

merenja pomoću

zatvorene petlje

Brojevi patenata:

4947339 (SAD);

0372429 (Evropa);

2117492 (Japan).

PRINCIP RADA

Page 13: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

PRINCIP RADA

Page 14: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

NAČINI POSTAVKE

EKSPERIMENATA

Page 15: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

NAČINI POSTAVKE EKSPERIMENATA

OTVORENI

SISTEM ZATVORENI

SISTEM

ANAEROBNI

SISTEM

Page 16: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

ZATVOREN SISTEM

Closed flow operation - tehnička i softverska priprema

Page 17: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Open flow operation

Za veoma aktivne uzorke

Meri se nivo vazduha koji prolazi kroz boce i

koncentracije gasova na ulazu i izlazu iz boca.

Potom, iz te tri koncentracije se preračunava

produkcija/konzumacija gasova.

OTVOREN SISTEM

Page 18: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Tehnička i

softverska

priprema

Početni protok

300 mL/min

(podešava se po

potrebi).

OTVOREN SISTEM

Page 19: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Anaerobic operation

(tehnička i softverska

priprema) 2 načina :

1. metoda sa dve boce;

2. metoda sa azotom.

Metoda sa 2 boce:

Reaktor boca →

jednosmerna veza → boca

za uzorkovanje →

„expansion interface“

ANAEROBNI SISTEM

Page 20: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

KALIBRACIJA

Page 21: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

KALIBRACIJA

Pre svakog merenja

Svi senzori imaju dve tačke kalibracije:

1. 0 (nula) gasa;

2. Raspon („span“) gasa

Prvo proveriti

senzore:

FILE →

PROPERTIES

Page 22: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

KALIBRACIJA

Potom: TOOLS → CALIBRATION → START

CALIBRATION

Podesiti protok na 500 mL/min → OK

Connect bottle 4...

Page 23: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

KALIBRACIJA

NEXT GAS...

Page 24: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

SOFTVERSKA

POSTAVKA

EKSPERIMENTA

Page 25: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

SOFTVERSKA POSTAVKA

EKSPERIMENTA

CHAMBER

SET UP

EXPERIMENT

SET UP

COMMENTS RUN

Page 26: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

EXPERIMENT SET UP

Page 27: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Start/End Chamber

Refresh

Auto Volume Measurement

Purge sensors

Switch Driers – kod dugotrajnih eksperimenata

O2 Consumtion positive

Enable Open Flow Mode

Anaerobic venting mode

EXPERIMENT SET UP

Page 28: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Sample Interval – minimum 12 min (6 min.

uzorak + 6 min. referentna boca)

Experiment Duration – N.A. (run→stop)

Data Units – gas units (μL, mL, mg, μM), time

units (min,h), normalization units (preračunava po

masi životinje)

Manualy Enter Chamber Temps. – kada postoji

više od dve temperaturne probe

Aux(iliary). Temp. Start. Chanel – daje se

komanda koji kanal je vezan za koji termometar

EXPERIMENT SET UP

Page 29: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

CHAMBER SET UP

Page 30: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Utilities

1. Merenje zapremina (Volume test):

a) Merenje zapremine senzora (250-320 mL);

b) Merenje zapremine boca (samo za V do 2 L).

2. Provera curenja sistema (Leakage test)

- Normalna vrednost je +/- 0,5 mL/min.

CHAMBER SET UP

Page 31: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

COMMENTS

Page 32: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

SOFTVERSKO

PRIKAZIVANJE

REZULTATA

Page 33: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

SOFTVERSKA POSTAVKA EKSPERIMENTA

I PRIKAZIVANJE REZULTATA

CHAMBER

SET UP

EXPERIMENT

SET UP COMMENTS

RUN DATA GRAPHING

Page 34: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

DATA

Page 35: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Int Colum. – interval;

Ch Colum. – kanal, odnosno, broj boce;

Time Culumn. – vreme uzorkovanja;

Ch_Temp. – temperatura u boci tokom tog ciklusa merenja;

RQ (RER) – Respiration Exchange Ratio (ΔCO2/ΔO2);

Gas % - Koncentracija gasa u boci tokom ciklusa merenja;

Gas rate – nivo proizvodnje (+) / potrošnje gasa (-);

Gas acumulation – ukupna količina promene gasa od početka eksperimenta.

DATA

Page 36: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

DATA STATUS

R - refresh

+ CP – pritisak u boci viši od uobičajenog opsega

- CP – pritisak u boci niži od uobičajenog opsega

+ PP – pritisak u senzorima viši od uobičajenog opsega

- PP – pritisak u senzorima niži od uobičajenog opsega

+ CO2 – nivo ovog gasa viši od normalnog opsega

merenja senzora

- O2 - nivo ovog gasa niži od normalnog opsega

merenja senzora

Page 37: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

GRAPHING

Page 38: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

REZULTATI

Page 39: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Aromatični ugljovodonici su grupa cikličnih ugljovodonika. Podeljeni su na monociklične ugljovodonike tzv. BTEX jedinjenja (BTEX, eng. Benzene, Toluene, Ethylbenzene, Xylene) i na policiklične aromatične ugljovodonike (eng. Polycyclic aromatic hydrocarbons - PAH).

DEGRADACIJA AROMATIČNE FRAKCIJE

DIZELA

Procenjuje se da se u prosečnom uzorku

sirove nafte nalazi 67% benzenskih, 18%

naftalenskih, 8% fenantrenskih, 3%

hrizenskih i benzfluorenskih, 2% pirenskih

i oko 1% antracenskih struktura.

Page 40: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Respiracija soja Rhodoccocus

sp. NR31 praćena je tokom 7

dana.

Korišćena je mineralna podloga

sa 1000 ppm aromatične

frakcije izolovane iz dizela kao

izvora ugljenika.

Koncentracija kiseonika i

ugljen-dioksida praćena je u

intervalima od 5h, a sistem je

bio zatvoren.

DEGRADACIJA AROMATIČNE FRAKCIJE

DIZELA - METODA

Page 41: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

DEGRADACIJA AROMATIČNE FRAKCIJE

DIZELA - GRAFICI

Page 42: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

DEGRADACIJA AROMATIČNE FRAKCIJE

DIZELA - GRAFICI

Page 43: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Lignin čini između 20-30%

drveta, u zavisnosti od vrste.

Nalazi se u ćelijskim

zidovima drveća u

kompleksu sa celuloznim i

hemiceluloznim

polisaharidima

Da bi se proizvela celuloza

pogodna za proizvode od

papira, potrebno je efikasno

odvajanje lignina od

celuloznih vlakana – u tom

procesu kao

nusproizvod nastaje

kraft lignin

DEGRADACIJA KRAFT LIGNINA

Page 44: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

Respiracija sojeva gljiva i Gram negativnih

bakerija tokom rasta na ligninu praćena je tokom

30 dana

Korišćena je mineralna podloga sa 0,25% lignina

kao jedinim izvorom ugljenika.

Koncentracija kiseonika i ugljendioksida

praćena je u zatvorenom sistemu.

DEGRADACIJA KRAFT LIGNINA - METODA

Page 45: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

DEGRADA

CIJA

KRAFT

LIGNINA:

GRAFICI

Page 46: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

PUBLIKACIJE

Page 47: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

PUBLIKACIJE

Page 49: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM

HVALA

NA

PAŽNJI!

Page 50: PowerPoint Presentationchem.bg.ac.rs/zf/lect/ZF2018_Loncarevic.pdf · Title: PowerPoint Presentation Author: Branka Kekez Created Date: 11/21/2018 12:37:48 PM