Overview of exosome strucrure
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Transcript of Overview of exosome strucrure
Overview of exosome strucrure
Archeal exosome
Rrp41
Rrp42
Rrp4Csl4
Components of the core protein of exosome complex
Rrp45p
Archaeal exosome
Two RNase PH subunits: Rrp41 and Rrp42
Two S1 homologs subunit : Rrp4 and Csl4
Rrp42 Rrp41RNase PH RNase PH1 1275 248
19 18728 224
1 223
Rrp4
20 50 57 124 135 220
1 179
Cls4
9 47 58 122 145 165
All-β S1 KH
All-β S1 ZR
S. solfataricus
The archaeal exosome core is a hexameric ring structure with three catalytic subunits
41(mut):D182A
42(mut):E218A
NATURE STRUCTURAL & MOLECULAR BIOLOGY VOLUME 12 NUMBER 7 JULY 2005
Pink : phosphate binding site
Sequence alignment of Rrp41 and Rrp42
View of the S. solfataricus Rrp41-Rrp42 Exosome Core Bound to a Short Single-Stranded RNA
The RNA is bound with the 3’ end near the phosphate binding site and with the 5’ end toward the central channel.
Molecular Cell, Vol. 20, 473–481, November 11, 2005
Detailed view of the structure at the RNA binding site
Schematic of the interactions at the RNA binding site
RNA Binding Arginines Are Essential for Catalytic Activity
Structure of exosome bound A5, U8 and ADP
polyA5 + Pi polyA4 + ADP
Schematic of the reaction mechanism
How does the RNA reach the active site?
Archaeal exosome
Two RNase PH subunits: Rrp41 and Rrp42
Two S1 homologs subunit : Rrp4 and Csl4
Rrp42 Rrp41RNase PH RNase PH
Rrp4 Cls4
All-β S1 KH All-β S1 ZR
Molecular Cell, Vol. 20, 461–471, November 11, 2005
Archaeoglobus fulgidus
Molecular Cell, Vol. 20, 461–471, November 11, 2005
Structure of the nine subunit archaeal Rrp4- and Csl4- exosomes Archaeoglobus fulgidus
[A] top view
[B] side view
[C] top view
Detailed view of the interface of the N-terminal domain of Rrp4 (red), Cls4 (red) and the C-terminal helix of Rrp41 (blue)
Rrp4-Rrp41 Cls4-Rrp41
Archaeal exosome V.S. bacterial PNPase
PNPase
Archaeal exosomeRrp42 Rrp41
KH-S1Rrp4 (Csl4)
RNase PH RNase PH
RNase PH RNase PH
Align RNase PH domain of Rrp41, Rrp42 and PNPase
PNPase RNase PH RNase PH
Structure, Vol. 8, 1215–1226, November, 2000
The structure of S. antibioticus PNPase with the phosphate analog tungstate bound to the at the PNPase catalytic sites