Thi lieu tham khao

1. Ban loth luyen, ciao to duqc lieu Trung QuOc (1965), K5 thuat nuoi !rang va the bien duvc lieu, nisei dich Nguyen Van Lan va cOng skr, Nxb N8ng nghiep, HA Nei. 2. Bao cao tai hOi nghi Duce lieu toan quer (2003), "Phat tridn dint lieu ben Ong trong the k)", 21". 3. Vien duqc lieu (2002), Cay thuOc va cleng vet lam thuac a Viet Nam, Nxb Khoa hoc va k9 thufit HA NOi. 4. Nguyen Van Thufin, 2004, Bcio cao icing kit khoa hoc va 197 thuet de tai nhanh czia de tai dec lap cap nha nwec "TrOng khao nghicFm met so cay thuOc can that cung cap &roc lieu de scin xucit thuOc tpi cho a met so dja ban nong them, mien mil tinh Thanh Hoe. 5. www.plantextra.com.

Tap chi Dirac lieu, Op 19, so 1/2014 (Trani; 46 - 52)

NHAN GIONG CAY DINH LANG LA NHO BANG KY THUAT NUOI CAY CHOI DINH

Le Thi Nhw Thad% Hang Hi& Thad , Mai Ti:! Phwang Hod, flo Tan Vinh2, TrAn Van Minh3

'DO clgi hoc Notig Lam thanh pha HO Chi Minh ; 2Dgi hoc Nguyin Tut Thanh, 3Dgi hoc Quoc ti, Dgi hoc Quoc gia thanh phd Ho Chi Minh

*Email: letnhuthao@gmail.com; drminh.ptntd@yahoo.com (Nh4rt bai ngay 31 than 12 nam 2013)

Tem tit ChOi dinh dinh lang (Jai 2-3 cm (cay 4 nam tu6i) dirge sir ding lam nguyen lieu nuoi cay. Choi duqc kha

trOng bang mrac javen n6ng d6 75 % trong thai gian 15 phut, cho hieu qua cao voi Au v8 tiling dot 70,17 % va t9 le to chOi dot 55,26 % sau 14 ngay nuoi cay. Moi tnrang LV thich hqp han cho skr sinh twang va phat tritn cua chOi dinh lang, the hien qua cac chi tieu se chi phat sinh (3,10), chieu cao chili (3,83 cm/chei), so la (8,54), chieu clai IA (2,24 cm/la) va chieu rOng la (0,35 cm/la) tot han so vai tren mai trutmg MS. Wei twang thich hqp cho nhan nhanh chi in vitro IA LV b6 sung BA (0,3 mg/I) va sucrose (30 g/1) cho hinh thanh 4,36 chei/mAu, chi pith trien du co than vuan cao (2,45 cm/ch6i), than la to khoe sau 40 nay nuoi cay. Mai tnrtmg LV co be sung IBA (0,3 mg/I) va sucrose (30 g/l) thich hqp cho nuoi cay too re dot ts, le 50,18 %, re dai tuyet del (2,50 cm), co5 s6 luqng re 1,39 re/chei sau 40 ngay nuoi cay. DA xfiy dung he thong nhAn giOng in vitro cay dinh lang

Tu kh6a: Polysciasfruticosa, ChOi dinh, Vi Man giOng, Phat sinh too re, Viral? warn. Summary

Micropropagation of Dinh !ling (Polysclas frutkosa L. Harms) by Shoot-tip Culture Technique Shoot-tip of pindle dinh lang with size of 2-3 cm was used as planting materials. The shoot-tip was sterilized

by javel 75 % in 15 minutes. Good results were obtained with 70.17 % of sterilization samples and 55.26 % of shoots rising after 14 days of culture. LV medium was favored for growth and development with 3.1 shoot/explant, 3.83 cm height/shoot, leaves number 8.54 and it was better than cultivation on MS medium. LV medium supplemented with BA (0.3 mg/I) and sucrose (30 g/l) was favored for in vitro multiplication with 4.36 shoots/explant and 2.45 cm height/shoot with leaves growth and development after 40 days of cultivation. LV medium supplemented with IBA (0.3 mg/I) and sucrose (30 g/I) was favored for root initiation 50.18 %, maximum root length 2.50 cm and root number 1.39 after 40 days of cultivation. A micropropagation system of dinh lang was set up

Keywords: Polyscias fruticosa, Shoot-tip, Micropropagation, Root initiation, Greenhouse.

1. Wein cay nhan sam, con dugc goi la "cay sam at". Cay dinh lang (Polyscisa fruticosa L.Harms) Cay dinh lang duac trong nhiL b vimg Dong

thuOc hQ ngu gia bi (Araliaceae) cOng hQ von nam A va Chau A Thai Binh Ducmg nhu

Fr 46 Top chi Dirac lieu, tap 19, s61/2014

Lao, Malaysia, Indonesia, Myanma, Papua New Guinea, Philippin, Singapore, Thai Lan va Viet Nam. Trong than, la, re c6 chug hap chAt saponin, d4c bit triterpen c6 tac dung ire the tao thanh malonyl dialdehyd trong qua trinh,peroxyd boa chong stress va trieu chimg tram cam. Hap chat polyacetylen, hederagenin, cac glycosid dm acid oleanolic co tac dung chong ung thtr, chong oxy h6a, khang khuAn va Ichang nAm [6,10]. Dugc sir dung nhieu trong y h9c co truyen Viet Nam va Trung Quec thay the cho nhan sam [1].

Hien nay, nhu cau sir dung cac san pham dinh Fang to nhien trong y - dugc ngay cang cao nham nang cao sirc khoe va sire chili dung dm ca the va phong thong nhieu loai benh, Ka qua nghien ciru dugc ly cua Vien y h9c quail su Viet Nam (1964) cho thA'y sir dung bOt dinh lang 0,23-0,50 &gay lam tang sire deo dai va chili dung cho ca the [4]. S6 luang va chAt Luang boat chAt thu nh4rt dugc tir to nhien Lai phu thuec vao nhieu yeu to nhu gi6ng loci, mita vu, the nhtrer'ng, th6i diem thu hoach.

Cong nghe to bao thuc vat da dugc ung

dung thanh ding trong viec nghien ciru phat sinh hinh thai cay dinh lang [7], nuoi cay re va to bao [3,13] cac loci cay dugc lieu nham thu nhan cac chAt c6 boat tinh dugc h9c. Nuoi cAy to bao sam My (Panax quinquefolium) thu nhan hoat chAt saponin [11]. Dueng va auxin c6 vai tre quan trong trong viec san xuat cac chat phenol trong nuoi cay to bao Panax ginseng [2]. Polyscias filicifolia da dugc nuoi cay mo seo va to bao bang ky thua't bioreactor thu nhOn hoat chAt glycosid dm acid oleanolic, da dugc san xuAt va dua vao thuang mai san phAm Witagmal' sau khi qua thir nghiem ye sinh h9c va lam sang [5]. Da thanh cong trong nhan gieng Polyscias filicifolia qua nuoi cAy phoi soma [14]. Cay dinh lang dugc trong trong dan gian v ri ba loci chinh, trong do cay dinh lang la nhe [14] co ham Wang hoat chAt cao, Lai co eat it trong ttr

nhien va kho khan thu hat de nhan gi6ng. Nhan gieng cay dinh lang la nhe bang ky thua't nuoi cAy chi dinh nham bao ten nguen gen va phat trien ngu6n tai nguyen dugc lieu coy nghia khoa h9c va thuc tan.

Hinh 1. Dinh lang Polyscias fruticosa (L.) Harms (A) Dinh Wig Ia nho, ria than xam tring, Ia 2 Ian kep, c6 rang cua

(B) Dinh Wig rang, than xdm tring, Ia 2 Ian kdp, c6 rang cua (C) Dinh lang clia, Ia to xanh

Tap chi Dra/c lieu, tap 19, s6 112014 47

2. Nguyen lieu va plInrong phip Nguyen lieu: Cay dinh lang dugc cung

cap tir Trung tam nghien ciru Sam va Dugc lieu (hinh 1) va ch6i nggn dugc sir dung lam vat lieu khoi dau cho nhan gi6ng.

Mai truang khoang ca ban: dugc sir dung cho nghien ciru Murashige-Skoog MS [12], Litvay LV [9], Loyd McCown WPM [8]. Cac chAt 136 sung vao moi twang nuoi cAy g6m: duing sucrose (30 g/1), BA (benzyladenine), NAA (a-napthaleneacetic acid), IAA ((3- indol acetic acid), IBA ((3-indol butyric acid), mac dira (15%). Chit khir Wing nuac javen (NaC10, niing dO pha tang 25-100 %).

Dieu kien nuoi cay: nhiet dO phong nuoi cay 26+2 'C, dO Am 70 %, cuang (10 chieu sang 22,2 gmol/m2/s, the dO 14 Oa chieu sang va 8 gia tai. Moi truang dinh dtra-ng nuoi cAy dugc khir trong o 1 atm trong thai gian 20 phut.

Phuang phdp: Thi nghiem dugc b6- tri theo kh6i ngeu nhien hoan chinh. Thi nghiem dugc lap 'al 3 Ian, moi Ian 3 binh tam giac (300 ml) chira 50 ml moi truang nuoi cAy, mai binh dugc nuoi cay 3-5 Au. Ket qua nghien ciru dugc xir ly thong ke bang phuang phap phan tich phuang sai mot chieu (P < 0,05) vai phan mem MSTATC.

Thiit kg thi nghiem Tao milli va ming: Doan ch6i dinh dai 2-3

cm (cay 4 nam tu6i) dugc rira bang nuac may de loai be cac loaf chAt ban. Sau do, khir trUng bang dung dich Javel Ming dO 25-100 trong 10-20 phut. Mau dugc nuoi cAy trong moi twang MS (1962) khong co chAt dieu hoa sinh tnrang [7].

Anh huong caa mai truang khoang din nuoi cay chai dinh: 136 tai sinh ch6i, mAu vo thing dugc chuyen sang nuoi cAy trong cac

moi twang khat nhau MS, LV, WPM khong c6 b6 sung chAt dieu hoa sinh truang.

All Throng caa BA din nhan chai: chi don c6 2 cap la dugc nuoi cAy trong moi truang nhan chi chira ming dO BA (0-1,2 mg/1) de xac dinh moi tnrorng thich hgp nhAt cho nhan nhanh ch6i.

A'nh huang caa IBA, IAA va NAA din ra ri. ch'ai Dinh Lang: choi dinh Lang (2-3 cm) dugc cAy chuyen sang moi truang tao re chira ming di, chAt dieu boa sinh truang IBA (0,1-0,5 mg/1), IAA (0,1-0,5 mg/1) va NAA (0,1-0,5 mg/1) de xac dinh moi twang thich hgp tao re.

Ccic chi lieu theo Ty le matt vo tang = (s6 tau cAy vo

triing/eang s6 rau khir trUng) x 100 TS, le mall tao chi = (s6 mkt vo trimg

phat sinh ch6i/ s6 mau cAy) x 100 S6 chi phat sinh = s6 chi tai sinh/ mau

nuoi cay TS, le chi ra re = (s6 chtii ra re/ tong s6

chi nuoi cAy) x 100 Cac chi tieu khac nhu chieu cao ch6i, s6 la,

kich thuac la, s6 re, chieu dai re. 3. Ket qua va thin) 1u4n To mAu vo trang Ket qua nghien ciru tao inAu chi v8 Wing

(Bang 1) cho thAy khir trong bang nuac javen n6ng dO 75 % trong thai gian 15 phut cho hieu qua cao vai mkt vo trung dat 70,17 % va tj, le tao chi dat 55,26 % sau 14 ngay nuoi cAy. Thai gian khir trong cang ngan (10 phut) va n6ng d6 javen thAp (50 %) cho tj, le mau vo triing thAp (12,9 %). Thai gian khir trong cang cao (20 ph6t) va nOng d6 javen cao (75 %) cho tj, le matt v8 trong cao (64,03 %) nhung irc the kha nang tao choi cua cay (45,38 %).

48 'Rip chi &tor lieu, tap 19, so 112014

Bang I. Anh haolw, cua chat khir trung nutc javel va th6i gian khir trung den tj, Ig mkt vo trung

va matt tao ch6i in vitro

Dung -----

Wing d(i (%) 25

dick Javen Thai gian

(phut)

TS, It; mAu vo

trung (% ) --.

0,00'

TS, ic Ian tao chi (0/0) Decdiem mAu

Cay nhiem nam va khuan 10

15

0,00'

25 0,00' 5,00' Cay nhiem dm va khuin 25 20 0,00' 10,00h Cay nhiem nam va khuan 50 10 12,9h 18,I8f Nhiem nam sau 3 - 4 Win 50 15 I9,04g 16,676 Nhiem nam sau 3 - 4 man

_

50 20 30,47f 20,15` Nhiern nam sau 3 - 4 man

75 10 43,75* 25,194 Cay Oat trien binh thutmg, 10 ngay too choi

75 15 70,17' 55,26' Clay phat trien binh thtdrng, 14 nghy tao ch6i

75 20 64,036 45,386 Cay phat trier) ch'am, 14 ngay t40 choi

100 10 54,14" 35,90c Cay phat trien chOm,17 ngay to chiii

100 15 60,28` 20,23e Cay phat trien cham, 17 ngay 40 ch6i

100 20 65,296 10,34h Cay Oat trien cham, 20 ngay too chlSi

a,b,c: Ccic ky tie theo sau gia tri trung binh trong cung mit cOt the hien sit khcic Net gift cac nghiem auk vol tmiv nghla P

Anh huirng dm mai truirng khofing den nuoi cay choi dinh

Ch6i dinh dinh lang vo trimg dirge tach va nuoi cay ten moi tru6ng MS, LV va WPM 136 sung sucrose 30 g/l, khong chira chat dieu him sinh trWmg, nham xac dinh mei throng khoang ca ban thich hap cho nhan ch6i dinh lang in vitro. Sau 30 ngay nuoi ea),

tren moi truong MS, LV va WPM (Bang 2), ch6i khong phat trien ca ve chieu cao tan kith thu6c la tren moi tnromg WPM; nguac 14i, ch6i phat trien tren hai moi

tru6ng MS va LV. Moi tru6ng LV thich hap han cho su sinh truing va phat trien cim chOi dinh Wig the hien qua cac chi tieu s6 ch6i phat sinh (3,10), chieu cao ch6i (3,83 cm/ch6i), so la (8,54), chieu dai la (2,24 cm/la) va chieu Ong la (0,35 cm/la) tot han so voi tren moi tru6ng MS (v6i cac ket qua 1L lua't la 1,08; 2,87;7,23;1,16 va 0,26 cm). Moi truimg LV ducc sir dung lam moi tru6ng ca ban de.

nghien ciru nhan nhanh ch6i in vitro; mei trutmg MS thich hap cho nuoi cAy phoi soma [14].

Tap chi Dtqic lieu, tap 19, st; 112014 49

Bang 2. Anh htrang cua moi trutmg khoang den nuoi cAy chi dinh in vitro

Mai truirng

nuoi cay

r SO chiii phat sinh /mAu

(ch6i) Chieu cao choi (cm)

SO Ia (la)

Kich thtrerc

Dai (cm)

Ia

ROng (cm)

D4c diem chei

MS 1,08b 2,874' 7,23b 1,16b 0,26a Cay phat vitt) chAm

LV 3,10 3,83' 8,54 2,24' 0,35' Than khoe, Ia xanh dam, to, phat trien choi

Than la yang khong phat trien WPM 0,00' 1,33b 5,73` 0,67' 0,24'

Anh huirng ciia BA den nhan chi Choi dinh dinh lang dirge nuoi cAy tren

mai twang LV c be sung BA vai cac nen dO khac nhau (0-1,2 mg/1), de xac dinh dinh nOng d8 chAt dieu h6a sinh twang thich hop cho nhan nhanh chOi. Sau 40 ngay nuoi cAy (Bang 3) tren moi truing LV b6 sung BA 0,1 mg/1 cho CS/ le to chi thAp nhAt: 2,2 chei/mAu (d.6i vii mAu chi dinh), chi phat trien tot than vtran cao (1,81 cm/chOi) va la

xanh. Moi threw LV be) sung BA 0,3 mg/1 cho hinh thanh 4,36 chei/mau, chi phat trien deu than vtran cao (2,45 cm/chei), than la to khoe; Khi tang neng do BA 0,6-1,2 mg/1, chi c6 xu htrong tao cum, than chi khong vtran cao, than la nh6. Nhu vAy, neng (10 BA b6 sung vao moi twang 0,6-1,2 mg/1 c6 tic dung kim ham vuan than ch6i. Moi truing thich hop cho nhan nhanh chi in vitro la LV b6 sung BA (0,3 mg/1) va sucrose (30 g/1).

Bang 3. Anh twang dm BA den nhan nhanh chei in vitro

BA (mg/I)

Chid dinh

Chieu cao chi (cm)

D#c diem chi SO chi phat sinh /cum (clad)

0 1,25r 1,20' Than Yuan thing, Ia to, tao chili it

0,1 2,20d 1,81` Than wan thing, la to, tao chi it

0,3 4,36' 2,45' Than man tlAng, Ia to, phat trier) deu 0,6 3,65" 1,92b Than Ia to khoe, cay giam ',hat trier) 0,9 3,01' 1,85bc Than phat trien chAm, Ia Me, yeu.

1,2 2,06' 1,52" Than Oat trien cham, la nho

Anh htribig cita IBA, IAA va NAA den ra re ciia chi dinh rang

De xac dinh moi truing nuoi cAy thich hop cho tao re, chi co kich thtrac 2 cm mang la dtrvc tach r6i va nuoi cAy ten mai truing LV c6 b6 sung IBA (0-0,5 mg/1), IAA (0-0,5 mg/1), NAA (0-0,5mg/1). Ket qua nghien ciru (Bang 4) cho thAy: Re khong c6 kha nang phat

sinh i nghiem thirc khong 136 sung chAt dieu hoa sinh twang (doi chtrng). Teen moi twang LV be) sung IBA 0,1 mg/1, chi cling khong phat sinh tao re. Khi tang nOng TO IBA len 0,3 mg/I cay bat dAu xuAt hien re sau 30 ngay nuoi cAy va dat ty le 50,18 %, re dai (2,50 cm), co so luting re 1,39 re/chOi sau 40 ngay nuoi cAy. Teen moi tnrerng LV be) sung IAA 0,1-0,5

50 Tap chi Ditor lieu, tap 19, se; 112014

mg./1 chi khong phat sinh re. Sau 30 ngay nuoi cay. ch6i bat dau ra rc trong moi true/mg LV co b8 sung NAA 0,1-0,5 mg/1; voi n6ng d8 NAA 0,3 mg/1) dat ty le tao re 40,51 %, s6 re 1,18 re-/ch6i. Nhu vay, moi twang LV c

b6 sung IBA (0.3 mg/1) va sucrose (30 g/1) la moi truing thich hop cho nuoi cay tao re cay dinh tang. Cay hoan chinh da duov chuyen tr6ng trong bau dat va duy tri trong nha luai sau 2 nam (Hinh 2).

Hinh 2. Nhan ging cay dinh lane bang ky thuat nuoi cay me 4 bao (a) Nutii cay choi dinh (b) Nhan chOi in vitro (c) Ch6i tao re (d) Cay cay mo 2 nam tuOi

Bang 4. Anh hudmg ctia IBA va NAA to re chi in vitro

Chat dieu hba sinh trtrang

Niing de (mg/1)

TS, le chi tao re

(%) s6 re ich6i

(a) Chieu dai re

trung binh (cm) DOi chimg 0,0

0,I

0,00r

o,00f

50,182

o,00b

0,0ob 1,392

0,00d

o,00d IBA

IBA 0,3 2,50' IBA 0,5 40,31` 1,25 2,236 IAA 0,I

0,3

coo'

0,00f

()slob 0,0ob o,00b

o,00d

0,00d o,00d

IAA

IAA 0,5 o,00f NAA 0,1 20,04' 0,0ob 0,00d NAA 0,3 40,5 I b 1, 1 85 2,452 NAA 0,5 30,52d I ,03a 1,58'

4. fat lutin Cay dinh lang dugc nhan ging thanh cong

bang ky thuat nuoi cay mo to bao. Ch6i dinh cita cay dinh lang duov khfr tin bang nuorc javen (n6ng di) 75 %) trong 15 phut cho hieu

qua khir trung cao nhat. Moi truing thich hop cho tai sinh la LV. Nhan nhanh chi tren moi tnrong LV co b6 sung BA (0,3 mg/1). MCA truing LV b6 sung IBA (0,3 mg/I) cho ty le ch6i phat sinh tao re cao nhat (50,18 %).

Tai lic;tu than Iihdo

1. Nguyen Tran Chau, DO Mai Anh, Nguyen Phtrcmg Dung (2007), Nghien dru met so tac dung dtrgc ly thrc nghiem ctia san pham cay mo tir cay dinh lang Polyscias pruticosa Harm Araliacea. Top chi nghien thu y hoc thanh phi): 1-1j Chi Minh,11(2):126

-131. 2. Ahn CH, Yoon WM (1996), Effects of auxin and sucrose on cell

Tgp chi &tor lieu, tap 19, so" 112014 51

growth and production of phenolic compounds in cell suspension culture of Panax ginseng C.A. Meyer. Journal

of the Korean Society for Horticultural Science 37(2): 340 344. 3. Dicosmo F, Masawa M (1996), Plant Cell Culture Secondary Metabolism. CRC Press. 4. DO Tit Lcri (2009), Cay thu6c va vi thu6c Viet nam. NXB Y hoc. 5. Furmanova M, Nosov AM, Orensikov AV, Klushin AG, Starosciak B, Sliwinska A, Guzewska J, Bloch R (2002), Antimicrpbial activity of Polyscias illicifolia cell biomass extracts. Pharmazie (57):424-426. 6. Huan VD, Yamamura S, Ohtani K, Kasai R, Yamasaki K, Nham NT, Chau HM (1998), Oleane saponins from Polyscias fruticosa. Phytochemistry (47):451-457. 7. Le Thien This, VO thj Bach Mai (2005), Nghien ciru ve sr Oat sinh hinh thai trong nuoi cay in vitro cay Binh lang (Polyscias fructicosa). Tap chi phat tridn khoa hoc va cong nghe dai hoc que,c gia TP. He, Chi Minh. 8(11):47-51. 8. Litvay JD, Verma DC, Johnson MA (1985), Influence of a loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Report (4):325-328. 9. Loyd G, McCown B (1980), Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Comb. Proc. la Prop. Soc. (30):421-427. 10. Mitaine-Offer AC, Tapondjou LA, Lontsi D, Sondegam BL, Choudhary MI, Rahman A, Lacaille-Dubois MA (2004), Constituents isolated from Polyscias fulva. Sys.EcoL (32):607-610. 11. Mathur A, Shukla YN (1994), Saponin production in callus and cell suspension culture of Panax quinquefolium. Phytochemistry Oxford 35 (5): 1221 1225. 12. Murashige T, Skoog R (1962), A revised medium for rapid growth and bioassays with tobacco tissue cultures. PhysioL Plant. (15):431-497. 13. Narula A, Kumar S, Bansal KC, Srivastava PS (2004), Biotechnological approaches towards improvement of medicinal plants. Plant biotechnology and molecular markers, pp 78-116. 14. Sliwhiska A, Olszowska 0, Furmanowa M, Nosov A (2008), Rapid multiplication of Polyscias filicifolia by secondary somatic embryogenesis. In vitro Cell.Dev.BioL-Plant (44):69-77.

Trip chi Dttor lieu, Op 19, so 1/2014 (Trail; 52 - 57)

NGHIEN CU'U ANH HUtiNG CUA THtil VV DEN NHAN GIONG VO TiNH Ti. CU ME DAN SAM

Doo Van Nail*, Ngo Qu'jc Lute, Trait Danh Viet', Nguyin Trhn Le Khfic Hgol , Trinh Van Vuvngl , Le Tian Vinh2

Vien Dtrac lieu, 2Cyc an tam dux phiim - BQ Y to *Email: vannui102@gmail.com; luatvdl@gmail.com

(Nhin bai ngay 26 thing 12 nam 2013) Tom tit

Thi nghiem nhan giOng vo tinh dan sam tir cu 6 7 th6i vu khac nhau. Ket qua cho they, cong thirc giam vao ngay 15/9 cho 14 sting (61,11 %) vi tj11' xuit vein (98,18 %) cao Mat.

Ttr Idtha: Nhan gang vo tinh, Cu, Dan sam. Summary

Influences of Crop Seasons on Asexual Propagation of Salvia miltiorrhiza Bunge by its Roots The experiment on asexual propagation Salvia miltiorrhiza Bunge by its roots was performed for seven

different crop seasons. The results showed that the treatment of roots and cultivation on September, 15th gave the highest survival rate (61.11%) and the highest rate of good standard plant (98.18%).

Keywords: Asexual Propagation, Root, Salvia miltiorrhia

1. Mir du nhi&I loci duqc nghien ciru nhan ging bang Teen tht th6' gi6i ding nhu 6 Vi't Nam, cit: Khoai lang, khoai tay, thugc dugc... v6i

52 Top chi Duor lieu, tap 19, so 112014