Microtubules. Basic Structure -Tubulin has a bound GTP, that does not hydrolyze. -Tubulin may...
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Transcript of Microtubules. Basic Structure -Tubulin has a bound GTP, that does not hydrolyze. -Tubulin may...
Microtubules
Basic Structure
-Tubulin has a bound GTP, that does not hydrolyze.
-Tubulin may have bound GTP or GDP.
GTP GTP
tubulin heterodimer
• An -tubulin heterodimer is the basic structural unit of microtubules.
1. Acidic proteins, M.W 55 kDa each
• Both ά and β tubulin have a nucleotide binding site.
Website with an image of a 3-D reconstruction of the structure of an intact microtubule, based on cryo-EM and image processing (by the Visualization Group at Lawrence Berkeley National Laboratory, K. Downing's research group).
A microtubule is a polymer of ά and ß tubulins. It is a hollow cylinder, about 24 nm in diameter. The tubulin heterodimers join end-to-end to form protofilaments, with alternating & subunits. 13 protofilaments yields a helical arrangement of tubulin heterodimers.
seam
microtubule 3-start helix
-GDP -GTP
-GTP -GTP
+
Microtubule Structure
GTP GDP GTP GTP
protofilament ( ) (+)
Microtubules can undergo treadmilling, with:
• addition of tubulin heterodimers at the plus end
• dissociation of tubulin heterodimers at the minus end.
seam
microtubule 3-start helix
-GDP -GTP
-GTP -GTP
+
These associate laterally to form sheets, & eventually microtubules.
Heterodimers: subunits add at v the plus end, where -tubulin is exposed.
Dynamic instability:Microtubules may grow steadily & then shrink rapidly by loss of tubulin dimers at the plus end. A GTP cap stabilizes the plus end of a microtubule.
seam
microtubule 3-start helix
-GDP -GTP
-GTP -GTP
+
Microtubules
GTP must be bound to both & subunits for a tubulin heterodimer to associate with other heterodimers to form a protofilament.
Subunit addition brings -tubulin that was exposed at the plus end into contact with -tubulin.
This promotes hydrolysis of GTP bound to the now interior -tubulin. Pi dissociates.
The GTP on -tubulin does not hydrolyze.
GTP GDP GTP GTP
protofilament ( ) (+)
GTP GDP
taxol tubulin dimer
PDB 1JFF
minus end
plus end
View an animation depicting assembly of microtubules.
Then explore the structure of the ,-tubulin heterodimer, using Chime.
Doublet & triplet microtubules: The wall of one microtubule partly consists of the wall of an attached microtubule. The A tubule is a complete microtubule cylinder, made of 13 protofilaments. “Piggyback” B or C tubules are made of less than 13 protofilaments, usually 10.
A B
A B C
1. The microtubules in most cells extend outwards from a microtubule organizing center (MTOC) in which the (-) ends of microtubules are anchored.
2. In animal cells, the major MTOC is the centrosome, located adjacent to the nucleus near the centre of an interphase cell.
3. The centrosomes of most animal cells contain a pair of centrioles surrounded by amorphous pericentriolar material.
4. The proteins present: pericentrin, centrin, -Tubulin etc.
5. -Tubulin, nucleates microtubule assembly within the centrosome.
Microtubule Organizing Center (MTOC)
Role of the MTOC
• Control the number of microtubules in a cell
• The polarity of the microtubules• The no. of protofilaments that make up
the walls of the microtubules.• Time and location of their assembly
Functions of Microtubules
• Centriole structure
• Molecular Motors
• Cilia and flagella
• Mitotic spindle
Centrioles: cylindrical structures, found in pairs. Orientated at right angles to one another. The wall of each centriole cylinder is made of nine interconnected triplet microtubules.
Centriole Structure
They are not found in plants, many unicellular euk. And some animal cells eg. mouse eggs.
Centrioles at right angles, as in the centrosome.
cartwheel structure at one end
Centriolar microtubules are relatively stable.
Additional tubulins and other proteins, are either present in centrioles or required for their formation.
During cell division, the duplicated centrosome helps to organize the mitotic spindle.
astral microtubule
chromosomal microtubule
polar microtubule
centrosome
Mitotic Spindle
During interphase, the centrosome (MTOC) is usually located near the nucleus.
Microtubules grow out from the MTOC, forming a hub & spoke array, even during interphase.
nucleus
MTOC
microtubules
nucleus
MTOC
microtubules
With minus ends of most microtubules anchored in the centrosome, microtubules grow & shrink mainly through addition & loss of tubulin heterodimers at their plus ends.
Toxins & Drugs
Some toxins and drugs (all of which inhibit mitosis) affect polymerization or depolymerization of tubulin:
Taxol: extracted from the bark of a rare tree, YEW.
Anti-cancer drug,
Binds to and stabilizes microtubules.
Colchicine: extracted from the autmn crocus.
Binds tubulin & blocks polymerization, causes depolymerization.
Nocodazole causes depolymerization of microtubules.
Vinblastine: Anti-cancer drug
Causes depolymerization and formation of vinblastine-tubulin paracrystals.