Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices
description
Transcript of Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices
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Author: Gene Yu Co-Authors: Dr. AlexBlake
Dr. David EddingtonJuly 29, 2010
NSF Research Experiences for Undergraduates (REU) inNovel Advanced Materials and Processing with Applications in Biomedical, Electrical and
Chemical Engineering
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ObjectiveDesign localized and
automated delivery system for microfluidic brain slice devices (μBSDs)
Delivers chemicals/drugs and gases
Automatically mixes oxygen
Characterize delivery of chemicals through tissue Image taken from
Mohammed, J, Caicedo, H, Fall, C, & Eddington, D. (2008). Microfluidic add-on for standard electrophysiology chambers. Lab Chip, 8, 1048-1055.
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MotivationμBSDs allow in vitro study of:
NeurogenesisSynaptogenesisRegenerationProtein expressionResponses to physical trauma
Image taken fromhttp://www.koki.hu/cdnb/research/research.html
Rambani, K, Vukasinovic, J, Glezer, A, & Potter, S. (2009). Culturing thick brain slices: an interstitial 3d microperfusion system for enhanced viability. Journal of Neuroscience Methods, 180, 243-254.
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RelevanceChemical Delivery
Spatial resolution Current systems affect entire
slice with drug Desire stimulation of specific
areas
Oxygen DeliveryAutomatic Mixing
Saves money Pre-mixing is not required
AutomationMulti-tasking
Image modified fromhttp://www.imagingeconomics.com/issues/articles/MI_2006-08_01.asp
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Oxygen Mixing and DeliveryExperimental Set-Up
Y Connector
Output Tube
Valves Gas Feed Lines: 0% and 21%
Oxygen concentration measured by a NeoFox Fiber Optic Oxygen (FOXY) sensor
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Oxygen Mixing and DeliveryOutput
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Oxygen Mixing and DeliveryGraphical User Interface (GUI)
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Delivery CharacterizationμBSD Design
T Channel
Delivery Channel
Reservoir
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Delivery CharacterizationExperimental Set-up
μBSD
Valve
DI Water Lines T Channel
Vacuum Line
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Delivery CharacterizationNo Tissue Delivery
10ms 15ms 20ms 25ms
2x5ms 3x5ms 4x5ms 5x5ms
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Delivery CharacterizationLinear Relationships: No Tissue
Width (µm)
Width (µm)
860
1320
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Delivery CharacterizationLinear Relationships: With Tissue
Full Dose Chemical Delivery
Pulsing Dose Chemical DeliveryMaximum Intensities
0 2 4 6Number of 5ms Pulses
Inte
nsi
ty
Inte
nsi
tyIn
ten
sity
Time (min)
Time (min)
2
2
Maximum Intensities
0 10 20
30Valve Open Time (ms)
Inte
nsi
ty
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Delivery CharacterizationGUI
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ConclusionsOxygen Delivery
Precise and accurate results
Reliable oxygen switching
Chemical Delivery Demonstrated linear
relationship Area of effect: ~2mm x
2mm Selective and
independent valve control
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AcknowledgementsFunding
NSF-REU DoD-ASSURE EEC-NSF Grant # 0755115
REU Directors Christos G. Takoudis, Ph.D. Greg Jursich, Ph.D.
Research Advisor David Thomas Eddington, Ph.D.
Mentors Alexander Blake, Ph.D. Gerardo Mauleon