Lecture Plasma Membrane
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Transcript of Lecture Plasma Membrane
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Essentials ofThe Living World
First Edition
GEORGE B. JOHNSON
Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
PowerPoint® Lectures prepared by Johnny El-Rady
4 Cells
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4.1 Cells
Fig. 4.1 The size of cells and their contents
20 nm
20 m
20 mm 2 mm 0.2 mm
2 m
2 nm 0.2 nm
0.2 m
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Robert Hooke (1665)Examined a thin slice of cork tissueObserved honeycombed compartments he called cellulae (L, small rooms)
The term became cells
Matthias Schleiden and Theodor SchwannProposed the first two statements of the cell theory in 1838-39
The Cell Theory
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In its modern form, the cell theory includes three principles
The Cell Theory
1. All organisms are composed of one or more cells
2. Cells are the smallest living things
3. Cells arise only by division of a previously existing cell
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Cells range in size from a few micrometers to several centimeters
Most cells are small because larger cells do not function efficiently
It is advantageous to have a large surface-to-volume ratio
As cell size increases, volume grows much faster than surface area
Cell Size
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Fig. 4.2 Surface-to-volume ratio
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Visualizing Cells
Few cells can be see with the unaided eye
Fig. 4.3 A scale of visibility
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Visualizing Cells
We can’t see most cells because of the limited resolution of the human eye
Resolution is the minimum distance two points can be apart and still be seen as two pointsResolution of the human eye is 100
One way to increase resolution is to increase magnification, using microscopes
There are two main types of microscopes
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Light Microscopes
Use light as the illuminating source
m
m
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Light Microscopes
Use light as the illuminating source
m
m
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Electron Microscopes
Use a beam of electrons to produce the image
m
m
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4.2 The Plasma Membrane
Encases all living cells
Its basic structure is represented by the fluid-mosaic model
Phospholipid bilayer with embedded proteins
Fig. 4.4 Phospholipid structure
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4.2 The Plasma MembraneIn water, phospholipids spontaneously form a bilayer
Cell membranes contain zones called lipid raftsHeavily enriched in cholesterol
Fig. 4.5
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Two main types
Cell-surface proteinsProject from the surface of the membraneAct as markers or receptors
Transmembrane proteinsExtend all the way across the bilayerProvide channels in and out of the cell
Proteins Within the Membrane
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Fig. 4.6 Proteins are embedded within the lipid bilayer
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Fig. 4.9
4.3 Prokaryotic CellsThere are two major kinds of cells
ProkaryotesEukaryotes
Prokaryotes include bacteria and archaea
Over 5,000 species are recognized
Prokaryotes come in three main shapes
Rod
Spherical
Spiral
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Prokaryotes have a very simple architecture
Pilus
Fig. 4.8
Found in all prokaryotes
They lack a nucleus and organelles
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4.4 Eukaryotic Cells
Appeared about 1.5 billion years ago
Include all cells alive today except bacteria and archaea
Are larger than prokaryotic cells
Have a much more complex architecturePossess nucleus and a variety of organelles
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Fig. 4.10 Structure of a plant cell
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Fig. 4.11 Structure of an animal cell
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Fig. 4.18
The Endosymbiotic Theory
Proposes that mitochondria and chloroplasts arose by symbiosis from ancient bacteria
This theory is supported by a wealth of evidence
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Cell Movement
Essentially, all cell motion is tied to the movement of microfilaments and microtubules
Changes in the shape of microfilamentsEnable some cells to change shape quicklyAllow some cells to crawlCause animal cells to divide
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Cell Movement
Flagella and ciliaConsist of a 9 + 2 arrangement of microtubulesAnchored in the cell by a basal body
FlagellaLong and few in number
CiliaShort and numerous
Fig. 4.21b Cilia
Paramecium
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Fig. 4.21a Eukaryotic flagellum
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Moving Material Within the Cell
Eukaryotic cells have developed high speed locomotives that run along microtubular tracks
KinesinMotor protein that moves vesicles to the cell’s periphery
DyneinMotor protein that moves vesicles to the cell’s interior
Fig. 4.22
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4.9 Outside the Plasma Membrane
Cell WallsOffer protection and support
Fungal cell walls are made up of chitin
Plant cell walls are made up of cellulose
Fig. 4.24
Glues cells together
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4.9 Outside the Plasma Membrane
Extracellular MatrixA mixture of glycoproteins secreted by animal cells
Fig. 4.25
Links ECM to the cytoskeleton
Helps coordinate the behavior of all cells in a tissue
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4.10 Diffusion and OsmosisDiffusion is the movement of molecules down their concentration gradient
Fig. 4.26
Equilibrium
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Osmosis
Diffusion of water through a semi-permeable membrane
Solutes are substances dissolved in a solutionHyperosmotic solution contains higher concentration of solutes than the cellHypoosmotic solution contains lower concentration of solutes than the cellIsotonic solution contains equal concentration of solutes as the cell
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Osmosis
Fig. 4.27
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Osmosis
Movement of water into a cell creates osmotic pressure
This can cause a cell to swell and burst
Fig. 4.28 Osmotic pressure in a red blood cell
Normal shape
Shape in pure water
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4.11 Bulk Passage into and out of Cells
Large amounts of material can be moved in and out of cells by membrane-bound vesicles
ExocytosisDischarge of material from vesicles at the cell surface
EndocytosisThe plasma membrane envelops particles and brings them into the cell interior
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Exocytosis
Means by which hormones, neurotransmitters and digestive enzymes are secreted in animal cells
Fig. 4.30
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2. PinocytosisEngulfment of liquid material
Endocytosis
Fig. 4.29a
Has three major forms
1. PhagocytosisEngulfment of particulate material
Fig. 4.29b
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Fig. 4.31
3. Receptor-Mediated EndocytosisThe process is highly specific and very fast
How low density lipoprotein (LDL) molecules bring cholesterol into animal cells
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4.12 Selective Permeability
Cell membranes have selective permeabilityThey contain protein channels that allow only certain molecules to pass
Selective DiffusionAllows molecules to pass through open channels in either direction
Ion channelsIf the ion fits the pore, it goes through
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Facilitated Diffusion
Net movement of a molecule down its concentration gradient facilitated by specific carrier proteins
Fig. 4.32
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Facilitated Diffusion
The rate can be saturated
Fig. 4.32
It increases up to a certain level and then levels off
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Active Transport
The movement of molecules across a membrane against a concentration gradient
This is possible by the expenditure of energy
Two types of channels are mainly used
1. Sodium-Potassium Pump
2. Proton Pump
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The Sodium-Potassium Pump
Fig. 4.33
Uses the energy of one ATP molecule to pump 3 Na+ outward and 2 K+ into the cell
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The Sodium-Potassium PumpLeads to fewer Na+ in the cell
This concentration gradient is exploited in many ways, including
1. The conduction of signals along nerve cellsChapter 28
2. The transport of material into the cell against their concentration gradient
Coupled channels
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Fig. 4.34 A coupled channel Can enter against its concentration
gradient
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The Proton Pump
Fig. 4.35
This process is termed
chemiosmosisExpends metabolic energy to pump protons across membranes
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How Cells Get Information
Cells sense chemical information by means of cell surface receptor proteins
These bind specific molecules and transmit information to the cell
Cells sense electrical information by means of voltage-sensitive channels
These allow ions into or out of the cell in response to electric signals