Laporan Ekstraksi DNA
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ASTRIDSAFIRAIDHAM
a blog with many benefit
SKIP TO CONTENT HOME ABOUT
LAPORAN PRAKTIKUM : ISOLASI DNA
MAY 29, 2014 / ASTRIDSAFIRAIDHAM
LAPORAN PRAKTIKUM
GENETIKAPERCOBAAN II
ISOLASI DNA
NAMA : ASTRID SAFIRA IDHAM
NIM : H41113341
HARI/TANGGAL : KAMIS, 13 MARET 2014
KELOMPOK : VI (ENAM) B
ASISTEN : ANDRI NINDA
LABORATORIUM GENETIKA !URUSAN BIOLOGI
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS HASANUDDIN
MAKASSAR
2014
https://astridsafiraidham.wordpress.com/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/#contenthttps://astridsafiraidham.wordpress.com/https://astridsafiraidham.wordpress.com/about/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/https://astridsafiraidham.wordpress.com/author/astridsafiraidham/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/#contenthttps://astridsafiraidham.wordpress.com/https://astridsafiraidham.wordpress.com/about/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/https://astridsafiraidham.wordpress.com/author/astridsafiraidham/https://astridsafiraidham.wordpress.com/ -
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BAB I
PENDAHULUAN
I"1 L#$#% B&'##*
Deoxyribonucleic acidatau DNA meu!a"a# $e#%a&a "'m'a %a#(
!a)'#( !e#t'#( *a)am ma"+)u" +'*u!DNA meu!a"a# $e#%a&a %a#(
me#(a#*u#( '#-.ma$' (e#et'" ma"+)u" +'*u! *a' $atu (e#ea$' "e
(e#ea$' $e)a#ut#%a Ke$e)uu+a# DNA *a)am $uatu $e) a"a#
meme#tu" (e#.m e#.m me)'!ut' a('a# (e# %a#( -u#($'.#a)
mau!u# #.#-u#($'.#a) *a)am $e) .(a#'$me DNA (e#.m me)'!ut'
(e# *a#'#te(e# 3Su%., 2004
DNA a*a)a+ a$am #u")eat %a#( me#(a#*u#( mate' (e#et'" *a#
e-u#($' u#tu" me#(atu !e"ema#(a# '.).('$ $e)uu+ e#tu"
"e+'*u!a# $e5aa $e)u)e DNA te*a!at !a*a #u")eu$, m't'".#*'a,
*a# ")..!)a$ Pee*aa# "et'(a#%a a*a)a+ DNA #u")eu$ ee#tu"
)'#'e *a# ea$.$'a$' $a#(at eat *e#(a# !.te'# +'$t.#, $e*a#("a#
DNA m't.".#*'a *a# ")..!)a$ ee#tu" $'"u)a *a# t'*a"ea$.$'a$' *e#(a# !.te'# +'$t.# Se)a'# 'tu DNA m't.".#*'a *a#
")..!)a$ mem')'"' 5'' "+a$, %a'tu +a#%a me&a'$"a# $'-at$'-at %a#(
ea$a) *a' (a'$ 'u Se*a#("a# DNA #u")eu$ mem')'"' !.)a
!e&a'$a# $'-at *a' "e*ua .a#(tua D')'+at *a' .(a#'$me#%a,
$tu"tu DNA !."a'.t t'*a" mem')'"' !.te'# +'$t.# *a# ee#tu"
$'"u)a, $e*a#("a# DNA eu"a'.t ee#tu" )'#'e *a# mem')'"'
!.te'# +'$t.# 3K'$ma#, 2010
I$.)a$' DNA meu!a"a# )a#("a+ mem!e)aa' DNA Sa)a+ $atu
!'#$'!'$.)a$' DNA %a'tu *e#(a# $e#t'-u(a$' Se#t'-u(a$'
meu!a"a# te"#'" u#tu"mem'$a+"a# 5am!ua# e*a$a"a# eat
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m.)e"u) ".m!.#e##%a M.)e"u) %a#( mem!u#%a' eat m.)e"u)
e$a a"a# ea*a *' a('a# a&a+ tau#( *a# m.)e"u) '#(a# a"a#
ea*a !a*a a('a# ata$ tau#( Ha$') $e#t'-u(a$' a"a#
me#u#u""a# *ua ma5am -a"$' %a#( te!'$a+, %a'tu $u!e#ata#
!a*a a('a# ata$ *a# !e)et !a*a a('a# a&a+ 3Ra5+mat, 2012
I"2 T++# P&%-.##
A*a!u# tuua# %a#( a"a# *'5a!a' !a*a !e5.aa# '$.)a$'
DNA '#' %a'tu u#tu" me#(eta+u' 5aa mem'$a+"a# 3me#('$.)a$'
DNA *a' 2 e#'$ ua+ %a#( *'a*'"a# $ea(a' $am!e), $eta u#tu"
me#(eta+u' "ea"t'-a# *ete(e# *a)am !.$e$ '$.)a$' DNA !a*a
$am!e) ua+
I"3 #$+ # T$ P&%-.##
Pe5.aa# '#' *')a"$a#a"a# !a*a +a' Kam'$, 16 Maet 2014,
!u"u) 1400 7 1860 ITA Pe5.aa# '#' etem!at *' :a.at.'um
B'.).(' Da$a, ;uu$a# B'.).(', Fa"u)ta$ Matemat'"a *a# I)mu
Pe#(eta+ua# A)am, U#'
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BAB II
TIN!AUAN PUSTAKA
I$.)a$' DNA a*a)a+ !.$e$ !e#(e)uaa# DNA *a' tem!at#%a ea*a
3e"$ta"$' atau )'$'$ 'a$a#%a *')a"u"a# *e#(a# +.m.(e#a$' *a#
!e#ama+a# u=e e"$ta"$' atau u=e )'$'$ u#tu" me#5e(a+ DNA
u$a" 3Yu&.#., 200>
Pa*a $e) eu"a'.t'" tema$u" ta#ama# *a# +e&a# a('a# tee$a
*a' DNA ea*a !a*a #u")eu$ %a'tu .(a#e) %a#( *'!'$a+"a# *a'
$'t.!)a$ma *e#(a# mema# Nu")eu$ te*'' *a' 90 ? "e$e)uu+a#
DNA $e)u)e S'$a DNA a*a)a+ .(a#e) )a'# $e!et' m't.".#*'a *a#
")..!)a$ Kae#a DNA te*a!at !a*a #u")eu$, ma"a !e)u a*a#%a
met.*e !e)'$'$a# $e) $am!a' !ema#e#a# $e) D'ma#a met.*e
te$eut meu!a"a# a('a# *a' met.*e '$.)a$' DNA 3E).*, 2008
Pem'$a+a# DNA *a' mate' $e)u)e )a'##%a meu!a"a# +a) %a#($'(#'@"a# *a# me#(+au$"a# !e#%a))'#a# DNA me#a*' RNA *a#
ta#$)a$' RNA me#a*' !.te'# e)a#($u#( *a)am
".m!ateme#3 ua#( %a#( ee*a %a'tu $e5aa etuuttuut
*a)am #u5)eu$ *a# $'t.!)a$ma 3 E).*, 2008
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Te*a!at .(a#e).(a#e) emema# (a#*a *' *a)am $'t.!)a$ma,
tema$u" m't.".#*'a a'" !a*a tumu+a# mau!u# +e&a# O)e+
"ae#a 'tu !e)u *')a"u"a# '$.)a$' DNA !a*a ta#ama# *a# +e&a#
u#tu" me#(eta+u' *a# mem!e)aa' DNA *a' ta#ama# *a# +e&a#
te$eut Se) eu"a'.t'" mem')'"' DNA )e'+ a#%a", )e#("a! *e#(a#
".m!.#e#".m!.#e# )a'# DNA ta#ama# *a# +e&a# te$'m!a#
*a)am #u5)eu$%a#( teu#("u$ .)e+ mema# I$.)a$' DNA
meu!a"a# )a#("a+ %a#( te!at u#tu" mem!e)aa' DNA P'#$'!#%a
a*a *ua, %a'tu $e#t'-u(a$' *a# !e$'!'ta$'Se#t'-u(a$' meu!a"a#
te"#'" u#tu" mem'$a+"a# 5am!ua# e*a$a"a# eat m.)e"u)
".m!.#e##%a M.)e"u) %a#( mem!u#%a' eat m.)e"u) e$a a"a#
ea*a *' a('a# a&a+ tau#( *a# m.)e"u) '#(a# a"a# ea*a
!a*a a('a# ata$ tau#( Ha$') $e#t'-u(a$' a"a# me#u#u""a# *ua
ma5am -a"$' %a#( te!'$a+, %a'tu $u!e#ata# !a*a a('a# ata$ *a#
!e)et !a*a a('a# a&a+ Pe$'!'ta$' meu!a"a# )a#("a+ %a#(
*')a"u"a# u#tu" me#(e#*a!"a# $uatu ".m!.#e# *a' 5am!ua#
3A)et, 1994
DNA !a*a ma"+)u" +'*u! *a!at *''$.)a$' $e5aa $e*e+a#a
Pe#('$.)a$'a# DNA $e5aa $e*e+a#a *a!at *')a"u"a# *e#(a#
meme5a+"a# *'#*'#( $e), mema# !)a$ma *a# mema# '#t' a'"
$e5aa me"a#'" mau!u# $e5aa "'m'a&' I$.)a$' DNA meu!a"a#
$uatu te"#'" %a#( *'(u#a"a# u#tu" mem!e.)e+ DNA mu#', %a'tu
ta#!a !.te'# *a# RNA *a' $uatu $e) *a)am a'#(a# Peme5a+a#*'#*'#( $e) $e5aa me"a#'" *a!at *')a"u"a# *e#(a# !em)e#*ea#
atau !e#((eu$ me#((u#a"a# m.ta *a# !'$t') Se*a#("a# $e5aa
"'m'a&' *a!at *')a"u"a# *e#(a# !eme'a# *ete(e# Pe#ama+a#
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$au# 5a' *a# (aam *a!u a*a)a+ u#tu" me)'$'$"a# mema# '#t'
u#tu" me#(e)ua"a# '$' '#t' $e) %a#( e'$' DNA 3Ra5+mat, 2012
I$.)a$' DNA *a!at *')a"u"a# me)a)u' ta+a!a#ta+a!a# a#taa )a'#!e!aa$' e"$ta" $e), !emu#'a# DNA *a' e"$t$" $e) *a# !e$'!'ta$'
DNA Me$"'!u# '$.)a$' DNA *a!at *')a"u"a# *e#(a# ea(a' 5aa,
a"a# teta!' !a*a $et'a! e#'$ atau a('a# ta#ama# *a!at
meme'"a# +a$') %a#( ee*a, +a) '#' *'"ae#a"a# a*a#%a
$e#%a&a !.)'-e#.) *a# !.)'$a"a'*a *a)am ".#$e#ta$' t'#((' %a#(
*a!at me#(+amat !emu#'a# DNA ;'"a '$.)a$' DNA *')a"u"a#
*e#(a# $am!)e ua+, ma"a "a*a a' !a*a ma$'#(ma$'#( ua+
ee*a, *a!at meme' +a$') %a#( ee*ae*a !u)a Sema"'#
t'#((' "a*a a', ma"a $e) %a#( te)aut *' *a)am e"$ta" a"a#
$ema"'# $e*'"'t, $e+'#((a DNA %a#( te!et'$'!a$' u(a a"a# $e*'"'t
3K'$ma#, 2010
Pe#ama+a# *etee# *a)am '$.)a$' DNA *a!at me#%ea"a#
u$a"#%a mema#e $e), me)a)u' '"ata# %a#( *'e#tu" me)a)u' $'$'
+'*.-.'" *etee# *e#(a# !.te'# *a# )ema" !a*a mema#e
meme#tu" $e#%a&a )'!'* !.te'#*etee# ".m!)e"$ Se#%a&a
te$eut *a!at tee#tu" "ae#a !.te'# *a# )'!'* mem')'"' uu#(
+'*.@)'" *a# +'*.-.'", *em'"'a# u(a *e#(a# *etee#, $e+'#((a
*a!at meme#tu" $uatu '"ata# "'m'a 3K'$ma#, 2010
P'#$'! 7 !'#$'! *a)am me#('$.)a$' DNA 3T.+', 2012
1 me)'$'$ $e) $e5aa @$'", *e#(a# 5aa !e#((eu$a#
2 !eme5a+a# *'#*'#( $e)
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6 !eme5a+a# mema# $e)
4 !em'$a+a# DNA *a' a+a# %a#( )a'#
Da)am '$.)a$' DNA, a+a# %a#( "'ta (u#a"a# 'a$a#%aeu!a a'#(a# tumu+a# atau a'#(a# +e&a#, u#tu" 'tu )a#("a+
!etama %a#( +au$ "'ta )a"u"a# a*a)a+ meme5a+"a# a'#(a#
me#a*' $e)$e) %a#( ma#*'' P.$e$ *')a"u"a# $e5aa me"a#'" atau
@$'" *e#(a# me#umu" atau me#((eu$ a+a# %a#( a"a# "'ta
(u#a"a# *e#(a# m.ta atau )e#*e Ke*ua a*a)a+ meme5a+"a#
*'#*'#( $e) *a# mema# $e) )a!'$a# !emu#("u$ DNA $tu"tu
utama !eme#tu" mema# *a# *'#*'#( $e) a*a)a+ )ema", u#tu" 'tu
"'ta (u#a"a# *etee# *a# (aam *a!u Ke*ua a+a# '#' *'(u#a"a#
u#tu" me)ua#(' *a# meu$a" $e) $e+'#((a '$' '#t' $e) 3DNA '$a
"e)ua 3T.+', 2012
Ta+a! $e)a#ut#%a a*a)a+ !em'$a+a# DNA *a' a+a# %a#( )a'#
Pem'$a+a# *')a"u"a# *e#(a# me#((u#a"a# et+a#.)/a)".+.) *'#('#
e".#$e#ta$' 909? Et+a#.)/A)".+.) t'*a" me)aut"a# DNA *a#
eat e#'$ a)".+.) %a#( )e'+ '#(a# *a' a' memuat DNA #a'" *a#
me)a%a#()a%a#( *' !emu"aa# 3T.+', 2012
CTAB atau Cetyl trimethylammonium bromide meu!a"a# $ee#'$
*etee# %a#( *a!at me#*e(a*a$' *'#*'#( $e), *e#atua$' !.te'#,
mem'$a+"a# "a.+'*at, meu$a" mema# $e) *a# me)aut"a#DNA A!a')a *'#*'#( $e) te*e(a*a$' ma"a $emua '$' $e) *a!at
"e)ua tema$u" DNA *a# *')e!a$"a# "e *a)am
u=e e"$ta"$'Da)am !.$e$ '$.)a$' DNA ta#ama#, !e#ama+a#
$e#%a&a !ee*u"$' $e!et' me5+a!t.eta#.) *a!at me#5e(a+
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!.$e$ ."$'*a$' $e#%a&a -e#.)'" $e+'#((a me#(+amat a"t'
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Met.*e '#' t'*a" memutu+"a# 'a%a %a#( )e'+ ma+a)
*'a#*'#("a# *e#(a# me#((u#a"a# "'t Se)a'# 'tu, "e)e'+a# *a'
e"ta"$' '#' a*a)a+ !'ta DNA %a#(*'!.)e+ )e'+ tea) ')a
*'a#*'#()a# *e#(a# e"ta"$' met.*e -e#.) *a# ta#!a -e#.) A"a#
teta!', *a' +a$') *e#(a# met.*e '#' ma$'+ te*a!at !'ta $mea *a#
DNA %a#( *'+a$')"a# )e'+ $e*'"'t *a'!a*a e"ta"$' *e#(a#
me#((u#a"a# "'t Ke#*a)a %a#( umum tea*' *a)am e"$ta"$' CTAB
a*a)a+ a*a#%a '#+''t. !a*a '#a#(, e#*a+#%a ".#$e#ta$'
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METODE PERCOBAAN
III"1 A'#$ # B##
III"1"1 A'#$
A)at 7 a)at %a#( *'(u#a"a# *a)am !e5.aa# '#' a*a)a+ a)at
tu)'$ me#u)'$, tau#( ea"$', t'ma#(a#, (e)a$ aua, !'$au, ata#(
!e#(a*u", $!atu)a, )e#*e, !'!et tete$, *a# me$'#
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T'(a e#'$ )auta# *ete(e# "emu*'a# *'ma$u""a# "e*a)am
ma$'#(ma$'#( tau#( ea"$' %a#( e'$' u$ ua+ $ea#%a" 4
m)
8 Tama+"a# 1 $!atu)a (aam *a!u "e*a)am ma$'#(ma$'#(
tau#( ea"$', "emu*'a# *'a*u" +'#((a )aut
> Cam!ua# "emu*'a# *'$a'#( $ea#%a" 2 "a)' *e#(a# "eta$
$a'#(
9 :a)u *'tama+"a# et+a#.) 9 ? $ea#%a" m) "e*a)am
ma$'#(ma$'#( 5am!ua#
10 :auta# "emu*'a# *'+.m.(e#"a# *e#(a# me#((u#a"a#
me$'#
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DAFTAR PUSTAKA
A)et, B, 1994 Biologi olekuler Sel Edisi Kedua PT ame*'a
Pu$ta"a Utama ;;;;;;;a"ata
A$'$, 2010 De!nisi "solasi D#A dan anfaat "solasi
D#A+tt!//a$'$08Stu*e#t'!a5'* D'a"$e$ !a*a ta#((a) 18 Maet
2014, !a*a !u"u) 2600 ITA
E).*, S, 2008 $enetika Edisi Keempat E)a#((a ;a"ata
K'$ma#, 2010"solasi D#A Buah +tt!//"'$ma#>6&ee)5.m
D'a"$e$ !a*a ttttttttta#((a) 18 Maet 2014, !a*a !u"u) 2600 ITA
Ra5+mat, 2012 "solasi D#A% +tt!//a5+mat%u$u-).($!.t5.m
D'a"$e$ !a*a $$$$$$$ta#((a) 18 Maet 2014, !a*a !u"u) 2600
ITA
http://asris07/http://kirsman83.weebl/http://rachmatyusuf.blogspot.com/http://asris07/http://kirsman83.weebl/http://rachmatyusuf.blogspot.com/ -
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Su%., 2004 $enetika Strata &% U#'
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1"PEPAACarica papaya
(++, -#6%, %6)
(++, -#6%, %6)
2"T.#$Solanu
m lycopercium
(%6, ++, -#6%)
(++, -#6%, %6)
IV"1"2 T#&' P&*##$#
!ENISBUAH PERLAKUAN
HASIL PERCOBAAN
!UMLAHARNA AKTU
Pe!a%a
Caricapapaya
Dete(e#Buu"
Put'+ 7e#'#( Ce!at
Dete(e# Ca'Ku#'#( 7e#'#(
:amat
Dete(e# K'mKu#'#( 7!u5at Se*a#(
T.mat
Solanumlycopercium
Dete(e#Buu"
Put'+ 7e#'#( Ce!at
Dete(e# Ca' Be#'#( :amat
Dete(e# K'm Ku#'#( Se*a#(
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IV"2 P#5#
P'#$'! utama *a)am '$.)a$' DNA a*a t'(a %a"#'
!e#(+a#5ua# 3)'$'$, e"ta"$' atau !em'$a+a# DNA *a' a+a#
!a*at $e!et' $e)u).$a *a# !.te'#, $eta
!emu#'a# DNA Se)a'# 'tu a*a 2 !'#$'! )a(' *a)am me)a"u"a#
'$.)a$' DNA, %a'tu $e#t'-u(a$' *a# !e$'!'ta$' P'#$'! utama
$e#t'-u(a$' a*a)a+ mem'$a+"a# $u$ta#$' e*a$a"a# eat e#'$
m.)e"u) *e#(a# 5aa meme'"a# (a%a $e#t'-u(a) $e+'#((a
$u$ta#$' %a#( )e'+ eat a"a# ea*a *' *a$a, $e*a#("a#
$u$ta#$' %a#( )e'+ '#(a# a"a# te)eta" *' ata$ Te"#'" $e#t'-u(a$'
te$eut *')a"u"a# *' *a)am $eua+ me$'# %a#( e#ama me$'#
$e#t'-u(a$' *e#(a# "e5e!ata# %a#( e
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tee#tu" a#taa *ete(e# *e#(a# atat %a#( a*a !a*a ua+
Sete)a+ !e#ama+a# *ete(e# ta+a! $e)a#ut#%a %a'tu
!e#ama+a#a (aam *a!u $ea#%a" 1 $!atu)a "e ma$'#(ma$'#(
tau#( %a#( e'$' 5am!ua# ua+ *a# *ete(e#, tuua# *a'
!e#ama+a# (aam a*a)a+ u#tu" memu*a+"a# !em'$a+a# e#a#(
e#a#( DNA *a' 5am!ua# $e+'#((a e#a#(e#a#( te$eut a"a#
mu*a+ *'amat' Ha) '#' tea*' "ae#a Na *a)am (aam *a!at
meme#tu" '"ata# !a*a "utu #e(at'
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mau!u# "a#*u#(a# a' *a' ma$'#(ma$'#( ua+ t'*a" $ama Da'
!e5.aa# t'*a" *'!e.)e+ DNA mu#' me)a'#"a# +a#%a DNA "a$a
atau e#a#( 7 e#a#( +a)u$ 3$u!e#ata#t *a)am um)a+ %a#(
$e*'"'t $eta e#*a!a# !ut'+ %a#( te)'+at Da' "e*ua ua+ DNA
"a$a %a#( !a)'#( te)'+at a*a !a*a ua+ !e!a%a, +a) 'tu tea*'
a"'at "a#*u#(a# a' !e!a%a )e'+ $e*'"'t *a' t.mat $e+'#((a
e"$ta" *a# DNA "a$a %a#( *'+a$')"a# .)e+ !e!a%a )e'+ a#%a"
*a'!a*a ua+ t.mat, $e)a'# 'tu e#*a!a# !ut'+ !a*a ua+ !e!a%a
)e'+ a#%a" *a'!a*a ua+ t.mat
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BAB V
PENUTUP
V"1 K&56+'#
Da' !e5.aa# '$.)a$' DNA %a#( te)a+ *')a"u"a# *a!at
*'"eta+u' met.*e atau 5aa a(a'ma#a me#('$.)a$' DNA *a' ua+
%a#( *'a*'"a# $ea(a' $am!e) Ya'tu me)a)u 5aa !e#(+a#5ua#
3)'$'$, e"$ta"$', $eta !emu#'a# Pe#((u#aa# *ete(e# %a#(
ee*a *a)am !e5.aa# *')a"u"a# u#tu" me#(eta+u' *ete(e#
*a)am e#tu" a!a %a#( !a)'#( a"t'- *a)am me#(+a#5u"a#
mema# $e) !a*a $et'a! $am!e) ua+Sete)a+ *')a"u"a# !e5.aa#
te#%ata *ete(e# uu" me#(+a$')"a# DNA "a$a %a#( )e'+
te)'+at $eta e#*a!a# !ut'+ %a#( )e'+ a#%a"
V"2 S#%#
V"2"1 S#%# U$+ L#.%#$.%6+ Sea'"#%a )a.at.'um me#%e*'a"a# a)at %a#( 5u"u!
mema*a' $eta a)at %a#( 5u"u! a#%a", a(a !a"t'"um *a)am
ea)a# *e#(a# e@$'e#
V"2"2 S#%# U$+ A565$&
D'+aa!"a# a(a teu$ mem!e+at'"a# *a# mem'm'#(
!a"t'"a# $e)ama )a e)a#($u#(, a(a !a"t'"um ea)a# *e#(a#
a'"
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Ade puji setyawati Biologi inspirasi
Jumat, 14 November 2014
!"#A! $NA %ANA&AN
ISOLASI DNA TANAMAN
A*e Pu' Set%a&at' 1
D+ B+'#tat' S Ha$ta', M B'.me*2
Fe$t% Au)'%au Ra+ma+, S S'2
Nu(.+. A*' Mau)a#a6
I#*+'#a Re'+a##'$+a6
1 Ma+a$'$&a ;uu$a# B'.).(', Fa"u)ta$ Sa'#$ *a# Te"#.).(', U#'
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sa'i hi(au tersebut% )raktikum ini dilakukan dengan tu(uan agar praktikan mampumelakukan isolasi D#A dari tanaman Sa'i -i(au *Brassica sp%+% -asil setelah
penambahan kloroform dan isoamil alkohol *./0&+ &x 1olum sampel dandisentrifugasi terbentuk . fase yaitu fase a2oueous dan organik% Setelah
penambahan isopropanol dingin terlihat benang3benang tipis D#A%
Kata kunci 0 Tanaman, daun Sa'i hi(au, pemurnian D#A
I" D#5#% T&.%6
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De.G%'. Nu5)e'5 a5'* 3DNA meu!a"a# $e#%a&a "'m'a %a#( !a)'#( !e#t'#( *a)amma"+)u" +'*u! DNA meu!a"a# $e#%a&a %a#( me#(a#*u#( '#-.ma$' (e#et'"ma"+)u" +'*u! *a' $atu (e#ea$' "e (e#ea$' $e)a#ut#%a DNA eu"a'.t t'*a" +a#%a*'um!a' !a*a #u")eu$, teta!' *a!at *'temu"a# !a*a m't.".#*'a *a# ")..!)a$DNA %a#( *''$.)a$' *a' ")..!)a$ me#u#u""a# $'-at ee#tu" $'"u)a, te*'' *a'
u#ta' (a#*a, e!)'"a$' $em'".#$e
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De#(a# me#(eta+u' DNA ta#ama# $a&' ma"a *a!at me#(+a$')"a# !.te#$'%a#( 5u"u! e$a u#tu" me#(+a$')"a#
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N. ama Ketea#(a#
1 Dau# +a$')!e#umu"a# *a#*'tama+"a#
u=e e"$ta"$'*a# *'
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Su!e#ata# %a#(te)a+*'tama+"a#'$.!.!a#.) *'#('#1 G
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te$eut $u)'t *'!'$a+"a# *a' e"$ta" a$am #u")eat Pemu"aa# $e) u#tu"me#(e)ua"a# a$am #u")eat#%a !a*a !a"t'"um '#' *e#(a# 5aa me"a#'" %a'tu*'(eu$ *e#(a# t'! $te') !a*a tau#( m'". atau *a!at u(a me#((u#a"a# a+a#"'m'a %a'tu #'t.(e# 5a' u#tu" me#*e(a*a$' *a# me)aut"a# ".m!.#e# *'#*'#($e)
Pa*a $aat !.$e$ *e$tu"$' a'#(a# ta#ama# *a!at me#%ea"a# *e(a*a$'!a*a DNA *e#(a# a*a#%a a"t'
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A$am #u")eat a*a)a+ m.)e"u) +'*.@)'" *a# e$'-at )aut *a)am a' D'$am!'#('tu, !.te'# u(a me#(a#*u#( e$'*u +'*.-.'" %a#( me#(a"'at"a# !.te'# )aut*a)am !e)aut .(a#'" Be*a$a"a# $'-at '#', te*a!at eea!a met.*e*e!.te'#'$a$' e*a$a"a# !em')'+a# !e)aut .(a#'" B'a$a#%a !e)aut .(a#'"%a#( *'(u#a"a# a*a)a+ -e#.) atau ")..-.m %a#( me#(a#*u#( 4? '$.am') a)".+.)
Pe#((u#aa# ")..-.m '$.am') a)".+.) 3CIA e*a$a"a# !ee*aa# $'-at !e)aut.(a#'"
K)..-.m t'*a" *a!at e5am!u *e#(a# a' *a# "emam!ua##%a u#tu"me#*e!.te'#'$a$' e*a$a"a# "emam!ua# a#ta' !.)'!e!t'*a %a#( te*e#atua$'u#tu" ma$u" atau tem.')'$a$' "e *a)am -a$e a#taa ")..-.m 7 a' K.#$e#ta$'!.te'# %a#( t'#((' !a*a -a$e a#taa te$eut *a!at me#%ea"a# !.te'#me#(a)am' !e$'!'ta$' Se*a#("a# )'!'* *a# $e#%a&a .(a#'" )a'# a"a# te!'$a+!a*a )a!'$a# ")..-.m 3D.%)e, 1990
P.$e$ *e!.te'#'$a$' %a#( e-e"t'- e(a#tu#( !a*a e$a#%a -a$e a#taa
")..-.ma' P.$e$ '#' *a!at *')a"u"a# *e#(a# meme#tu" emu)$' *a' a' *a#")..-.m Ha) '#' +a#%a *a!at *')a"u"a# *e#(a# !e#((..(a# atau $e#t'-u(a$'%a#( "uat "ae#a ")..-.m t'*a" *a!at e5am!u *e#(a# a' I$.am') a)".+.)e-u#($' $ea(a' emu)$'@e *a!at *'tama+"a# "e ")..-.m u#tu" mema#tu!eme#tu"a# emu)$' *a# me#'#("at"a# )ua$ !emu"aa# ")..-.ma' %a#( ma#a!.te'# a"a# me#(a)am' !e$'!'ta$'
Pe#((u#aa# ")..-.m '$.am') a)".+.) '#' memu#("'#"a# u#tu" *'*a!at"a#DNA %a#( $a#(at mu#', #amu# *e#(a# u"ua# %a#( teata$ 32000070000 !DNA "emu*'a# *''"at *a' -a$e au.eu$ *e#(a# !e$'!'ta$' '$.!.!a#.) 3Mu)a*#.,2002 Sete)a+ !.$e$ e"$ta"$', DNA %a#( *'*a!at *a!at *'!e"at"a# me)a)u'
!e$'!'ta$' Pa*a umum#%a *'(u#a"a# '$.!.!a#.) *'#('# u#tu" me#(e#*a!"a#,me)e"at"a# *a# mem'$a+"a# DNA *a' )auta#
Pa*a $aat !e#ama+a# '$.!.!a#.) *'#('# te)'+at e#a#(e#a#( t'!'$ DNAta#ama# e&a#a !ut'+ %a#( me)a%a#( *' )auta# te$eut Ke*ua $e#%a&ate$eut a"a# mem!e$'!'ta$' DNA !a*a -a$e au.eu$ $e+'#((a DNA me#((um!a)meme#tu" $tu"tu @e *a# tee#tu" !e))et $ete)a+ *')a"u"a# $e#t'-u(a$'Me#uut He)*t 3200 a+&a !e$'!'ta$' e-u#($' u#tu" me#(+')a#("a# e$'*ue$'*u ")..-.m %a#( ea$a) *a' ta+a!a# e"$ta"$'
Me#uut Yu&.#., 3200> !'#$'!!'#$'! !e$'!'ta$' a#taa )a'# !etama,
me#uu#"a# "e)auta# a$am #u")eat *a)am a' Ha) '#' *'"ae#a"a# m.)e"u) a' %a#(!.)a me#(e)')'#(' m.)e"u) DNA *' )auta# au.eu$ Muata# *'!.)e !.$'t'- *a' a'e'#tea"$' *e#(a# muata# #e(at'- !a*a (u(u$ -.$-.*'e$te DNA I#tea"$' '#'me#'#("at"a# "e)auta# DNA *a)am a' I$.!.!a#.) *a!at e5am!u *e#(a# a',#amu# "ua#( !.)a *'a#*'#("a# a'
M.)e"u) '$.!.!a#.) t'*a" *a!at e'#tea"$' *e#(a# (u(u$ !.)a *a' a$am#u")eat $e+'#((a '$.!.!a#.) a*a)a+ !e)aut %a#( )ema+ a(' a$am #u")eatQ "e*ua,
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!e#ama+a# '$.!.!a#.) a"a# me#(+')a#("a# m.)e"u) a' *a)am )auta# DNA$e+'#((a DNA a"a# te!e$'!'ta$'Q "et'(a, !e#((u#aa# '$.!.!a#.) *'#('# a"a#me#uu#"a# a"t'
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DAFTAR PUSTAKA
*'a#a, D" 2009 Te"#'" '$.)a$' DNA (e#.m ta#ama# !a!a%a *a# eu" *e#(a#me#((u#a"a# m.*'@"a$' u=e CTAB% BuletinTeknik )ertanian 14(1): 121
.#ata 2008 Komunikasi pribadi% Ciri3ciri D#A murni dan penyebab keberhasilan sertakegagalan dalam )C4 dan elektroforesis%E)a#((a ;a"ata
.%)e ;; a#* D.%)e ;: 1990 I$.)at'.# .- !)a#t DNA -.m -e$+ t'$$ue F.5u$ M.$5.&1231161
ae), RE 2004 4#A ethodologies0 A 5aboratory $uide for "solation and Characteri6ation%Third Edition%E)$e
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Lukisan Karya'amis, 20 &aret 2014
Laporan Genetika Isolasi DNA
LAPORAN PRAKTIKUM
GENETIKA
PERCOBAAN II
ISOLASI DNA
NAMA : NUR AFIAH SULAIMAN
NIM : H41113704
KELOMPOK : V (LIMA) B
HARI/TGL" PERCOBAAN : KAMIS/ 13 MARET 2014
ASISTEN : RISK NURHIKMAANI
http://catatantentangbiologi.blogspot.co.id/http://catatantentangbiologi.blogspot.co.id/ -
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LABORATORIUM GENETIKA!URUSAN BIOLOGI
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS HASANUDDIN
MAKASSAR
2014
BAB I
PENDAHULUAN
I"1 L#$#% B&'##*
DNA 3De.G%'.$e Nu5)e'5 A5'* a*a)a+ m.)e"u) utama %a#( me#(.*e $emua
'#-.ma$' %a#( *'utu+"a# u#tu" !.$e$ meta.)'$me *a)am $et'a! .(a#'$me DNA
te$u$u# ata$ 6 ".m!.#e# utama %a'tu (u)a *e."$''u$a, a$a #'t.(e# *a# -.$-at
%a#( te(au#( meme#tu" #u")e.t'*a M.)e"u) DNA '#' te'"at meme#tu"
".m.$.m, *a# *'temu"a# *' #u")eu$, m't.".#*'a *a# ")..!)a$ 3A(u$ *a#
Sa-aae#a#, 2014
DNA %a#( me#%u$u# ".m.$.m '#' meu!a"a# #u")e.t'*a a#("a! %a#(
teu$u$# +e)'G a#*a 3*.u)e +e)'G, %a#( a$a #'t.(e# *a# "e*ua e#a#(
!.)'#u")e.t'*a $a)'#( e!a$a#(a# *a)am !a$a#(a# %a#( teta! me)a)' '"ata#
+'*.(e# *a# a#taa #u")e.t'*a %a#( $atu *e#(a# %a#( )a'# *'+uu#("a# *e#(a#
'"ata# -.$-at DNA te*a!at *' *a)am $et'a! $e" ma"+)u" +'*u! *a# *'$eut $ea(a'
5eta" 'u "e+'*u!a# "ae#a m.)e"u) '#' e!ea# !e#t'#( $ea(a' !ema&a
'#-.ma$' +ee*'ta$ %a#( me#e#tu"a# $tu"tu !.te'# *a# !.$e$ meta.)'$me )a'#
3A(u$ *a# Sa-aae#a#, 2014
DNA *a!at me#(a)am' *e#atua$' *a# e#atua$' Se)a'# 'tu, DNA u(a '$a
*''$.)a$' I$.)a$' DNA a*a)a+ $uatu te"#'" *'ma#a +a$') a"+'#%a $ta#*$ta#* DNA
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*a!at te!'$a+ *a)am e#tu" "um!u)a# $ta#* e&uu* e#a#(e#a#( !ut'+
Tuua# '$.)a$' DNA a*a)a+ me#*a!at"a# e"$ta" DNA !a*a a'#(a# atau $e) %a#(
*''#('#"a# 3A(u$ *a# Sa-aae#a#, 2014
I$.)a$' DNA '#' $a#(at !e#t'#( *a)am '*a#( '.te"#.).(' mau!u# '*a#(
'.).(' m.)e"u)e Pe#(e#a)a# '$.)a$' DNA $a#(at !e#t'#( me#('#(at '.te"#.).('
!a*a a"+'a"+' '#' $a#(at mau Te)e'+ u#tu" '*a#( '.).(' m.)e"u)e Beea!a
a"te' te)a+ e+a$') *''#t.*u"$' "e *a)am ta#ama# $e!et' !a*' "a!a$ *a# "e*e)a'
Pe#t'#(#%a '.te"#.).(' u#tu" !e"ema#(a# "ea#e"aa(ama# +a%at' *' ma$a
me#*ata#( meme)u"a# "eteam!')a# *a# !em'"'a# Sa)a+ $atu#%a a*a)a+ *e#(a#
me#(eta+u' 5aa !e#(um!u)a# DNA *a# !'#$'! *a$a#%a $ea(a' !'a"a# u#tu"
mem!e)aa' '.).(' m.)e"u)e !a*a "+u$u$#%a 3A(u$, *a# Sa-aae#a#, 2014
1"2 T++# P&%-.##
A*a!u# tuua# *a' !a"t'"um '#' a*a)a+
1 U#tu" me#(eta+u' 5aa atau met.*e %a#( e#a u#tu" mem'$a+"a# 3me#('$.)a$'
DNA *a' ua+ua+a#
2 Me#(eta+u' "ee-e"t'-a# *ete(e# *a# ua+ %a#( *'!a"a' u#tu" me)a"u"a#
!e5.aa# '$.)a$'
I"3 #$+ # T$
Pe5.aa# I$.)a$' DNA '#' *')a"$a#a"a# !a*a +a' Kam'$, 16 Maet 2014
!u"u) 14001800 ITA etem!at *' :a.at.'um B'.).(' Da$a, Fa"u)ta$
Matemat'"a *a# I)mu Pe#(eta+ua# A)am, U#'
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BAB II
TIN!AUAN PUSTAKA
Stu*' me#(e#a' e"$'$te#$' a$am #u")eat !etama "a)' *')a"u"a# .)e+
F'e*'5+ M'e$5+e *a' ;ema# %a#( me#('$.)a$' '#t' *a' $e) *aa+ !ut'+ !a*a ta+u#
1>9 M'e$5+e me#emu"a# a+&a *' *a)am '#t' $e) te$eut te*a!at $e#%a&a
%a#( me#(a#*u#( -.$-at %a#( "emu*'a# *'#ama"a# #u")e'# Se)a#ut#%a !a*a
a"+' aa* "e19 te)a+ e+a$') *')a"u"a# !em'$a+a# a#taa DNA 3deoxyribonucleic
acid *a# RNA 3ribonucleic acid *a' !.te'#!.te'# %a#( me)e"at"a# m.)e"u) a$am
#u")eat te$eut !a*a $e) Pa*a a&a) ta+u# 1960a#, P :e
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%a#( $ama *a# te(a" )uu$ te+a*a! a"$'$ u#ta'a# DNA U#ta'a# DNA mem!u#%a'
*ua )e"u"a# 3groo1e e"$te#a) %a'tu )e"u"a# e$a 3ma(or groo1e *a# )e"u"a# "e5')
3minor groo1e Ke*ua )e"u"a# te$eut mem!u#%a' !ea#a# $ea(a' tem!at
me)e"at#%a m.)e"u) !.te'# tete#tu 3Yu&.#., 200
U"ua# m.)e"u) DNA e
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*e#(a# ua+ e"a*a a' e#*a Sema"'# t'#((' "a*a a' ma"a $e) %a#( te)aut *'
*a)am e"$ta" a"a# $ema"'# $e*'"'t, $e+'#((a DNA %a#( te!e$'!'ta$' u(a a"a#
$e*'"'t 3A(u$ *a# Sa-aae#a#, 2014
P.$e$ '$.)a$' DNA *'a&a)' *e#(a# !.$e$ e"$ta"$' DNA Ha) '#' etuua#
u#tu" mem'$a+"a# DNA *e#(a# !at'"e) )a'# %a#( t'*a" *''#('#"a# P.$e$ '#' +au$
*')a"u"a# *e#(a# +at'+at', $e+'#((a t'*a" me#%ea"a# "eu$a"a# !a*a *'#*'#(
$e), mema# $e) mema# !)a$ma *a# mema# '#t' a'" *e#(a# 5aa me"a#'"
mau!u# $e5aa "'m'a&' Caa me"a#'" '$a *')a"u"a# *e#(a# !em)e#*ea# atau
!e#((eu$ me#((u#a"a# m.ta *a# !'$t') Se*a#("a# $e5aa "'m'a&' *a!at
*e#(a# !eme'a# %a#( *a!at meu$a" mema# $e) *a# mema# '#t', $a)a+
$atu#%a a*a)a+ *ete(e# 3A(u$ *a# Sa-aae#a#, 2014
Se)a'# 'tu, $e5aa "'m'a )'$'$ *'#*'#( $e) *a!at *')a"u"a#
*e#(a# me#ama+"a# $e#%a&a "'m'a $e!et' )'$.'m, EDTA 3et+')e#*'am'#
tetaa$etat, *a# SDS 3$.*'um
*.*e$') $u)-at Da)am +a) '#' -u#($' EDTA a*a)a+ $ea(a' !eu$a" $e)
*e#(a# 5aame#('"at ma(#e$'um I.# '#' e-u#($' u#tu" mem!eta+a#"a# '#te('t
a$ $e)
mau!u#mem!eta+a#"a# a"t'
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$'$' +'*.-.'" *ete(e# *e#(a# !.te'# *a# )ema" !a*a mema# meme#tu"
$e#%a&a )'!'* !.te'#*ete(e# ".m!)e"$ Se#%a&a te$eut *a!at tee#tu"
"ae#a !.te'# *a# )'!'* mem')'"' uu#( +'*.@)'" *a# +'*.-.'", *em'"'a# u(a
*e#(a# *ete(e# $e+'#((a *a!at meme#tu" $uatu '"ata# "'m'a Kemu*'a#!e#ama+a# (aam 3NaC) e-u#($' u#tu" me)aut"a# DNA *a# $ete)a+ 'tu
*'e'"a# et+a#.) *'#('# 9? u#tu" me#(um!u)"a#/me#((um!a)"a# DNA 3A(u$
*a# Sa-aae#a#, 2014
E)e"t.-.e$'$ DNA meu!a"a# te"#'" u#tu" mem'$a+"a# $am!e) DNA
e*a$a"a# ata$ u"ua# 3eat m.)e"u) *a# $tu"tu @$'" m.)e"u)#%a e) %a#(
'a$a *'(u#a"a# a#taa )a'# a(a.$a E)e"t.-.e$'$ (e) a(a.$a *a!at *')a"u"a#
u#tu" mem'$a+"a# $am!e) DNA *e#(a# u"ua# *a' eea!a atu$ +'#((a 20000
!a$a#( a$a 3! 3D&'*.$e!ut., 199>
M.)e"u) DNA emuata# #e(at'- $e+'#((a *' *a)am me*a# )'$t'" a"a#
em'(a$' me)a)u' mat'"$ (e) me#uu "utu !.$'t'- 3a#.*e Ma"'# e$a u"ua#
m.)e"u)#%a, ma"'# e#*a+ )au m'(a$'#%a Beat m.)e"u) $uatu -a(me# DNA *a!at
*'!e"'a"a# *e#(a# mema#*'#("a# )au m'(a$'#%a *e#(a# )au m'(a$' -a(me#
-a(me# m.)e"u) DNA $ta#*a 3DNA ma"e %a#( te)a+ *'"eta+u' u"ua##%a
V'$u)'$a$' DNA $e)a#ut#%a *')a"u"a# *' a&a+ !a!aa# $'#a u)ta
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METODE PERCOBAAN
III"1 A'#$
A)at %a#( *'(u#a"a# *a)am !e5.aa# '#' a*a)a+ !'$au, me$'# )e#*e,
t'ma#(a#, (e)a$ aua, !e#(a*u", !e#%a'#( 3t'$$u / "a!a$ / "eta$ $a'#(, $!atu)a,
tau#( ea"$', !'!et tete$, *a# me$'#
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8 Me#(amat' !.$e$ t'mu)#%a DNA, me)'!ut' &a"tu %a#( *'!e)u"a#, &a#a, $eta
a#%a" $e*'"'t#%a DNA %a#( tee#tu"
W
BAB IV
HASIL DAN PEMBAHASAN
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IV"1 H#56' P&%-.##
IV11 ama +a$') Pe5.aa#
;e#'$ ua+ See)um Se$u*a+
T.mat Dete(e#
Buu"
Dete(e#
5a'
Dete(e#
"'m
Pe!a%a Dete(e#
Buu"
Dete(e#
5a'
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Dete(e#
"'m
IV"1"2 T#&' P&*##$#
N. ;e#'$ Bua+ Pe)a"ua# Ha$') Pe#(amata# ;um)a+
a#a a"tu
1 T.mat
Solanumlycopersiu
m
Dete(e#
Buu"
Be#'#(, a*a
e#*a!a#
:amat
Dete(e#
Ca'
Be#'#( Se*a#(
Dete(e#
K'm
Put'+ Be#'#( :e'+
Ce!at
2 Pe!a%a
Carica papaya
Dete(e#
Buu"
Put'+
Ke"u#'#("u#'#(a#
:e'+
Ce!at
Dete(e#
Ca'
Put'+ "eu+ :amat
Dete(e#
K'm
Put'+ $u$u Se*a#(
IV"2 P#5#
Pa"t'"um '$.)a$' DNA '#' *')a"u"a# etuua# u#tu" me#(eta+u' !e#(au+
ma5am ua+ *a# e#'$ *etee# te+a*a! "ua)'ta$ DNA %a#( *'+a$')"a# *a)am
!.$e$ '$.)a$' Se+'#((a *a)am !a"t'"um '#' *'uat
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*ete(e# *a# e#'$ ua+ a(a *a!at me#(eta+u' ua+ *a# e#'$ *ete(e# ma#a
%a#( me#(+a$')"a# DNA !a)'#( a(u$ *a# a'" ;e#'$ ua+ %a#( *'(u#a"a# a*a)a+
t.mat Solanum lycopersicum$ea(a' 5.#t.+ ua+ %a#( me#(a#*u#( a#%a" a'
*a# !e!a%a Carica papaya$ea(a' ua+ %a#( mem')'"' "a#*u#(a# a' t'*a" te)a)ua#%a" Se*a#("a# u#tu" *ete(e# %a#( *'!a"a' a*a)a+ *ete(e# uu" me" Su-,
*ete(e# 5a' me" R'#$., *a# *ete(e# "'m me" BuCeam
Pa*a *a$a#%a !'#$'! '$.)a$' DNA a*a t'(a %a'tu !e)'$'$a# $e), e"$ta"$' *a#
!emu#'a# Pa*a !a"t'"um "a)' '#' %a#( !etamatama *')a"u"a# 'a)a+
me#(+a#5u"a# $am!e) ua+ *e#(a# 5aa me"a#'" atau !em)e#*ea# ua+ u#tu"
me)'$'$/ meu$a" *'#*'#( $e)% Seme#taa 'tu *'uat !u)a )auta# *ete(e# *e#(a#
5aa me#5am!u"a# "et'(a e#'$ *ete(e# 3Su-, R'#$. *a# Bu5eam "e *a)am
aua*e$ Pe#(a*u"a# *')a"u"a# *e#(a# +at'+at' a(a t'*a" tea*' a#%a" u$a
%a#( *a!at me#(+a)a#(' !e#(amata#
Kemu*'a# u$ ua+ *'tama+"a# *e#(a# )auta# *ete(e# Pe#ama+a#
)auta# *ete(e# e-u#($' u#tu" me)'$'$ *'#*'#( $e) tumu+a# %a#( te*a!at *a)am
)auta# $am!e) I#' *'$ea"a# "ae#a $'-at *a' *ete(e# $ama *e#(a# $'-at *'#*'#(
$e) %a#( +'*.-.'", $e+'#((a tea*' '"ata# *'a#taa "e*ua#%a *a# me#%ea"a#
*'#*'#( $e) u$a"
:a)u *'tama+"a# (aam *a!u *a# *'a*u" Pe#('$.)a$'a# DNA me#((u#a"a#
(aam *a!u *e#(a# tuua# u#tu" meme"at"a# DNA Ha) '#' *a!at tea*' "ae#a
'.# Na%a#( *'"a#*u#( .)e+ (aam mam!u meme#tu" '"ata# *e#(a# "utu
#e(at'
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teua' te$eut e*a$a"a# eat m.)e"u) Et+a#.) e!ea# *a)am !e#(um!u)a#
*a# !e#((um!a)a# DNA "ae#a $'-at#%a %a#( *'#('#
Kemu*'a# $ete)a+ *'amat' !.$e$ t'mu)#%a DNA, me)'!ut' &a"tu %a#(
*'!e)u"a#, &a#a, $eta a#%a" $e*'"'t#%a DNA %a#( tee#tu" Ma"a +a$') %a#(
*'*a!at"a# %a'tu )auta# %a#( !a)'#( 5e!at meme#tu" (um!a)a# DNA a*a)a+
t.matBu"'m *a# t.matSu-
Ha) '#' et.)a" e)a"a#( *e#(a# te.' %a#( me#%ata"a# a+&a ua+ !e!a%a
a"a# )e'+ a#%a" me#(+a$')"a# (um!a)a# DNA "ae#a mem')'"' "a#*u#(a# a'
%a#( )e'+ $e*'"'t *a'!a*a t.mat $e+'#((a DNA %a#( te!e$'!'ta$' )e'+ a#%a"
!a*a ua+ !e!a%a
Ke#%ataa# '#' u(a t'*a" $ea)a# *e#(a# a#((a!a# *ete(e# %a#( !a)'#( a'"
*'(u#a"a# u#tu" '$.)a$' DNA a*a)a+ *ete(e# uu", "emu*'a# *ete(e# 5a', )a)u
*ete(e# "'m Da' +a$') te$eut ma"a %a#( *a!at *'$'m!u)"a# a+&a t.mat *a#
BuCeam !a)'#( a#%a" me#(+a$')"a# DNA Da# *a' "et'(a e#'$ *etee# %a#(
*'(u#a"a#, %a#( !a)'#( $e*'"'t me#(+a$')"a# DNA a*a)a+ *etee# R'#$. :auta#
*e#(a# *etee# uu" Su- *a# Bu "'m me#(+a$')"a# DNA *e#(a# um)a+ %a#(
5u"u! a#%a" Namu# "et'(a e#'$ *etee# te$eut t'*a" *a!at *'a#*'#("a#
ma#a %a#( )e'+ a'" *a)am me#('$.)a$' DNA "ae#a !a*a $et'a! !e)a"ua#
me#u#u""a# +a$') %a#( e)at'- $ama
Kemu#("'#a# te*a!at "e$a)a+a# !a*a $aat *')a"u"a# !em)e#*ea# ua+
!e!a%a %a#( te)a)u )ama *a# te)a)u +a)u$ $e+'#((a DNA %a#( *'*a)am#%a
te5a'"5a'" +a#5u *a# me#%ea"a# "e(a(a)a# !a*a $aat !a*a '$.)a$' DNA
Se)a'# 'tu !a*a $aat !.$e$ !e#%a'#(a# *a# !e#ama+a# et+a#.) tea*' "e+a'$a#
a+a# *a# "ae#a#%a te*a!at $ea('a# !e5.aa# %a#( e)um tu#ta$ *a#
me#((u#a"a# a+a# !e#((a#t' %a#( teata$ "a!a$'ta$#%a *'a#*'#("a# a+a#
%a#( $eme$t'#%a *'(u#a"a# *a)am !a"t'"um
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BAB V
PENUTUP
V"1 K&56+'#
A*a!u# "e$'m!u)a# *a' !a"t'"um '#' a*a)a+
1 U#tu" me)a"u"a# '$.)a$' DNA met.*e atau te"#'" %a#( *'(u#a"a# !a*a *a$a#%a
a*a t'(a %a'tu !e)'$'$a# $e), e"$ta"$', *a# !emu#'a# Pe)'$'$a# *a!at *')a"u"a#
*e#(a# 5aa me"a#'" mau!u# "'m'a, $a)a+ $atu#%a *e#(a# 5aa *')e#*e *a#
*'5am!u )auta# *ete(e# E"$ta"$' *a!at *')a"u"a# *e#(a# !e#ama+a# (aam
*a!u 3NaC) *a# !emu#'a# $a)a+ $atu met.*e#%a a*a)a+ *e#(a# meme'"a#
)auta# et+a#.) *'#('# 9?
2 ;e#'$ *ete(e# *a# ua+ %a#( *'!a"a' u#tu" me)a"u"a# '$.)a$' DNA $a#(at
e!e#(au+ !a*a DNA %a#( *'+a$')"a#
V"2 S#%#
A*a!u# $aa# u#tu" !a"t'"um '#' $ea'"#%a a)at *a# a+a# )a.at.'um
*'tama+ u#tu" me#5e(a+ tea*'#%a "e"ua#(a# a+a# %a#( *a!at
me#(a"'at"a# "ea$a)a+a# *a# "ua#( a"uat#%a +a$') %a#( *'*a!at"a# !a*a
!a"t'"um '#'
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DAFTAR PUSTAKA
A(u$, R.$a#a *a# Sa-aa#a'# 2014 )enuntun )raktikum $enetika%Ma"a$$a
D&'*.$e!ut. 199> Dasar3Dasar ikrobiologi Damata# ;a"ata
K'ma)), ; ;.+# 1992Biologi E)a#((a ;a"ata
Mu)a*#. 2002 Seputar Teknologi 4ekayasa $enetika Pu$ta"a 'au$a+a Mu*a *a#
USESE F.u#*at'.# B.(.
Su+a$.#. *a# '*%a$tut', Utut 200 )elatihan Singkat Teknik Dasar )engklonan $en
IPB Pe$$ B.(.
Yu&.#., T'&'.&. 200 Biologi olekular E)a#((a ;a"ata