Jesper Bartholin Bruhn,1* Allan Beck Christensen,2 Lars Ravn Flodgaard,1
description
Transcript of Jesper Bartholin Bruhn,1* Allan Beck Christensen,2 Lars Ravn Flodgaard,1
Presence of Acylated Homoserine Lactones (APresence of Acylated Homoserine Lactones (AHLs) andHLs) and
AHL-Producing Bacteria in Meat and PotentialAHL-Producing Bacteria in Meat and PotentialRole of AHL in Spoilage of MeatRole of AHL in Spoilage of Meat
Jesper Bartholin Bruhn,1* Allan Beck Christensen,2 Lars Ravn FlodgJesper Bartholin Bruhn,1* Allan Beck Christensen,2 Lars Ravn Flodgaard,1aard,1
Kristian Fog Nielsen,3 Thomas Ostenfeld Larsen,3 Michael Givskov,Kristian Fog Nielsen,3 Thomas Ostenfeld Larsen,3 Michael Givskov,22
and Lone Gram1and Lone Gram1
Received 26 September 2003/Accepted 8 March 2004Received 26 September 2003/Accepted 8 March 2004
學生學生 ::吳俊昌吳俊昌
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2004, p. 4293–4302 Vol. 70, No. 7
IntroductionIntroduction
Enterobacteriaceae Enterobacteriaceae 腸道菌腸道菌 種類 例種類 例 ::
Hafnia alvei Hafnia alvei 718718腐肉中最多 腐肉中最多
Serratia spp.Serratia spp.次多次多
Serratia proteamaculansSerratia proteamaculans B5a B5a產產 proteaseprotease 使牛奶使牛奶 coagulationcoagulation
IntroductionIntroduction
Monitor bacteriaMonitor bacteria
Agrobacterium tumefaciens Agrobacterium tumefaciens NT1(pZLR4)NT1(pZLR4)
AHL-reporter plasmidAHL-reporter plasmid
偵測有偵測有 oxo,6-14Coxo,6-14C
Chromobacterium violaceum Chromobacterium violaceum CV026CV026
偵測無偵測無 oxo,4Coxo,4C
IntroductionIntroduction
AHLAHL 有不同的有不同的 structure structure 例例 ::
N-3-oxo-hexanoyl homoserine lactone(OHHL)N-3-oxo-hexanoyl homoserine lactone(OHHL)腐肉中佔最多腐肉中佔最多比沒有比沒有 oxooxo 的利用率的利用率 ,, 效能及持久度高效能及持久度高
N-hexanoyl homoserine lactone(HHL)N-hexanoyl homoserine lactone(HHL)腐肉中次多腐肉中次多
N-octanoyl homoserine lactone(OHL)N-octanoyl homoserine lactone(OHL)
菌數濃度菌數濃度
分離菌種分離菌種
主要的主要的 AHLAHL 構型構型1.Marker
2.H. alvei 718
3.4 week 再萃取
4.3 week (fat)
5.2 week (fat)
6.1 week (low fat)
7. 當天 (fat)
利用 ESI+-MS 再度證實
AHLAHL 影響影響 phenotypephenotype
1.marker
2.718
3.718
4.718 halⅠ mutant
5.718 halⅠ mutant
6.718 halⅠ mutant + OHHL
7.718 halⅠ mutant + OHHL
8.marker
20kDa
Bacteria communicationBacteria communication
SummarySummary
Hafnia alvei Hafnia alvei 718 718 為主要為主要 以以 N-3-oxo-hexanoyl homoserine lactoneN-3-oxo-hexanoyl homoserine lactone
(OHHL)(OHHL) 為主要構型為主要構型718718 halⅠ halⅠ mutantmutant 影響影響 phenotypephenotypeBacteria communication(718 and B5a)Bacteria communication(718 and B5a)