1 The world leader in serving science

Next Generation Punching Systems for Reference DNA Samples 2014

Introducing CPA200 & CPA300

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Collection Punching Amplification Detection Analysis

3500 and 3500xL Genetic

Analyzers

GeneMapper® ID-X expert

system software

Direct PCR Amplification Kit

NUCLEIC-CARD™ Framed Cards

GeneAmp® PCR System 9700 Cycler Veriti® 96-Well Thermal Cycler

BSD® semi-automated punching systems

BSD® Automated punching systems

NUCLEIC-CARD™ Unframed Cards

Direct Amplification Workflow for DNA Databasing

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Why a Punching System?

Increase Throughput

Improve Lab

Efficiency

Minimize Operator

Errors

Enhance Chain-of- Custody Tracking

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Update on BSD® Punchers by Luminex

• On April 28, 2014 Luminex communicated to Life Technologies the plan to cease the operations of BSD and the manufacture of BSD® punchers

• On the same date, Luminex announced they would no longer accept any BSD® Puncher orders

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Life Technologies and BSD® Punchers

• Life Technologies has been a distributor of BSD® Punchers in the forensic market since the end of 2009

• Life Technologies recognizes the importance of service and support for deployed BSD® instruments

• Life Technologies plans to continue to offer its standard comprehensive service plan for the BSD® punchers until June 30th, 2016

• As a result of the Luminex decision, Life Technologies may need to offer more limited service

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Life Technologies & Copan

• Life Technologies started distributing Copan sample collection devices for forensic applications including the NUCLEIC-CARD™ system in 2012

• Life Technologies and Copan signed a distribution agreement in June 2014 to distribute the CPA technology developed by Copan

• Life Technologies is now the global exclusive distributor of CPA instruments in the human identification market

• Life Technologies will provide service and support for installed CPA instruments

NUCLEIC-CARD™ System FLOQSwabsTM Devices

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Introducing CPA200 and CPA300

CPA2001 Semi-automated puncher

CPA3001 Fully-automated puncher & liquid handler

1. Card Processing Automation

PC Operational Box Operational Box PC

Support Box

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CPA200 – General Specs

CPA2001 Semi-automated puncher

1. Card Processing Automation

Dimensions 610mm (L) x 545mm (W) x 675mm (H)

Weight 72 kg Peripherals Touchscreen,

keyboard, mouse, USB (x2)

Network Ethernet 1 Gbit/sec

Exportable files .pdf & .csv files

Available Late Summer 2014!

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CPA200 – Supported Cards

Optimized for NUCLEIC-CARD™ with Cleaning Area

• Validated punch size: 1.2 mm

• Additional punch sizes are currently under consideration

• Protocol with “cleaning punch” available

CPA200 works with sample collection systems routinely used in forensic laboratories: • NUCLEIC-CARD™ (regular and custom) • FTA® Cards (Whatman) • Buccal DNA Collector (Bode)

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CPA200 – Punch Quality

CPA200 includes: • Ionizer – to neutralize the electrostatic charges on the

puncher tool to ensure detachment of paper disks and release in the correct well

• Dust Remover – to remove any card debris from the puncher tool to ensure excellent cleaning conditions and optimal cutting quality

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Intuitive and User-Friendly User Interface

Real-time updates on plate status

Camera control of each well before and

after punching

Event List

Workflow

User Protocol Status

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CPA200 – Cross-Contamination Study

• Objective: Assess well cross-contamination when preparing a 96-well plate with CPA200

• Method: Collect samples from 23 donors using FLOQSwabs™ Regular Transfer samples onto 1 spot NUCLEIC-CARD™ COLOR, 4 spots

NUCLEIC-CARD™ COLOR, FTA® cards and Bokumbio cards. Cards were dried at RT for one hour Add 15 µL GlobalFiler® Express Reaction Mix1 to each well of the 96-

well plate Punch one 1.2 mm disc in each well of the plate followed by one

cleaning strike between punches Seal the plate and centrifuge it at 300 rpm for 20 seconds Amplify the samples using a GeneAmp® PCR System 9700

1. For Forensic or Paternity Use Only

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CPA200 – Plate Set Up 1 2 3 4 5 6 7 8 9 10 11 12

A NC1 NP8 NC9 NP15 NC17 NP22 NP29 NC29 NP37 NC36 NP44

B NP1 NC2 NC10 NP16 NC18 NP23 NC25 NP33 NC32 NP41 NC40

C NP2 NC3 NP9 NC11 NC19 NP24 NP30 NC30 NP38 NC37 NP45

D NP3 NC4 NP10 NC12 NP17 NC20 NC26 NP34 NC33 NP42 NC41

E NP4 NC5 NP11 NC13 NP18 NC21 NP25 NP31 NP39 NC38 NP46

F NP5 NC6 NP12 NC14 NP19 NC22 NP26 NC27 NP35 NC34 NC42

G NP6 NC7 NP13 NC15 NP20 NC23 NP27 NP32 NC31 NP40 NC39 POS

H NP7 NC8 NP14 NC16 NP21 NC24 NP28 NC28 NP36 NC35 NP43 NEG

Legend: • NP: Non-Probative samples • NC: Negative Control samples – Blank punches used as substrate control • NEG: Negative Control DNA well - No punches added to this well • POS: Control DNA well – No punches added to this well

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CPA200 – Electropherograms

• Representative electropherogram generated from NP well

• Full STR profile generated

• Representative electropherogram generated from NC well

• No STR profile generated

Data generated using Applied Biosystems® 3130 Genetic Analyzer

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CPA200 – Key Findings

• Good punch quality with different card types • No instances of incorrect barcode reading • No disc losses • Consistent release of the paper discs at the bottom of the

well as confirmed by the real-time feedback from the camera • No instances of cross-contamination • Full concordance between STR profile and expected profile

from the donor1 • Very user-friendly software

1. Samples from each donor were spotted on two cards. Each card was punched so that the discs associated with each donor randomly ended up in two different plate wells

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CPA300 – General Specs

CPA3001 Fully-automated puncher

1. Card Processing Automation 2. Operational box

Dimensions 1160mm (L) x 760mm (W) x 1005mm (H)2

Weight 270 kg Peripherals Touchscreen,

keyboard, mouse, USB (x2)

Network Ethernet 1Gbit/sec

Exportable files .pdf & .csv files

Available early Fall 2014!

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CPA300 – Supported Cards

CPA300 works with framed cards: • 51 mm x 51 mm (single collection area) • 85 mm x 53 mm (two collection areas)

Validated punch size: 1.2 mm

NUCLEIC-CARD™ Collection Device

NUCLEIC-CARD™ with Cleaning Area

Whatman EasiCollect

Custom Framed Cards

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CPA300 – Operational Box Overview

Sealing Unit

Tips Station

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CPA300 – Operational Box Overview

Storage

Punching Unit

Scanning Area Plate Gripper Card Gripper

Pipetting Unit

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CPA300 – Punching Unit

• Puncher has two main functions: generates a 1.2 mm disk that

is safely released in the 96 well plate performs the cleaning

procedure. The cleaning disk(s) are released in a separate bin

• Ionizer removes the electrostatic

charges

• Dust Remover removes card debris

Two cards per minute WITH

cleaning punch

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CPA300 – Plate and Card Grippers

• Plate gripper enables the movement of the plate during the punching and liquid handling procedures

CPA300 can accommodate up to three 96-well plates

Bin to collect cleaning punches

• Card gripper transports the card from and to the card rack to enable the punching procedure

CPA300 can accommodate up to four card racks1

1. Each rack fits up to 75 cards

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Sealing Unit

• Plates are automatically sealed at the end of the liquid handling procedure

Reduces risks of cross-contamination Helps prevent reagent

evaporation PCR-ready plate for better

efficiency

Full cycle: Approx. one hour per plate1

1. Includes punching, cleaning, liquid handling and sealing

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Collection Punching Amplification Detection Analysis

3500 and 3500xL Genetic

Analyzers

GeneMapper® ID-X expert

system software

Direct Amplification Workflow for DNA Databasing

Direct PCR Amplification Kit

NUCLEIC-CARD™ Framed Cards

GeneAmp® PCR System 9700 Cycler Veriti® 96-Well Thermal Cycler CPA200

CPA300

NUCLEIC-CARD™ Unframed Cards

28 The world leader in serving science

Please contact Andrea Carbonaro at andrea.carbonaro@thermofischer.com

For Research, Forensic or Paternity Use Only unless otherwise noted. Not for use in diagnostic procedures. STR kits described are for Forensic or Paternity Use Only. When used for purposed other than Human Identification the instruments and modules of the cited software are for Research Use Only. Not for use in diagnostic procedures.

The content provided herein may relate to products that have not been officially released and is subject to change without notice.

©2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. BSD is a trademark of Bizpac Party Ltd. FTA and EasiCollect are trademarks of Whatman Limited. NUCLEIC-CARD and FLOQSwabs are trademarks of Copan Flock Technologies Srl.

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