Photons, Pairs and Background for Nominal and LowP accelerator options
Incident Photon Flux (μmol m -2 s -1 ) Photons Absorbed and Utilized (μmol m -2 s -1 ) Photons...
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Transcript of Incident Photon Flux (μmol m -2 s -1 ) Photons Absorbed and Utilized (μmol m -2 s -1 ) Photons...
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Incident Photon Flux (μmol m-2 s-1)Phot
ons
Abso
rbed
and
Util
ized
(μm
ol m
-2 s-1
)
Photons absorbed
Excess
Photons utilized at 25 °C
Photons utilized at 10 °C
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Before Damage of PSII Degradation of PSII Following Repair of PSII
LipidsUndamaged PSII
Damaged PSII LHCII
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time
Photosystem II Supercomplexes
Free Antenna Complexes
Damaged Photosystem II Monomers
grana stroma lamellae
4 ns
0.5 ns?Immediately After Damage
Before Damage
?Minutes After Damage
4 ns
0.5 ns
Tens of Minutes After Damage
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STED beam 730 nm
Excitation beam660 nm
x-axis
y-axisSample
Fluorescence680-720 nm
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Excess Sunlight
250 500 750 1000 1250
Photons Utilized Sunlight
250
500
750
1000
1250
Incident Photon Flux (μmol m-2 s-1)
Phot
ons
Abso
rbed
and
Util
ized
(μm
ol m
-2 s-1
)
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Without qE With qE
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Laser pulse
Laser pulse
DetectorPhotosynthetic Sample
Photosynthetic Sample
Fluorescence Photons Detector
Shutters
We can digitally control when the measurement is made to measure changes in energy transfer as different conditions areapplied to the sample