Improved methods for in situ enzymatic amplification and detection of low copy number genes in...

9

Transcript of Improved methods for in situ enzymatic amplification and detection of low copy number genes in...

  • !

    " # $

    % &' &(()* +' &(()

    , -

    .-%#/0 -%#/

    !

    /,1 2 3

    2

    "# * - * !* "* - * -

    4

    5 6&7 2 3

    4 * 3

    5

    # 3

    4 , 5

    4

    &89+8 4

    6+'7

    .-:%0 3

    .-%#/0

    6+9(7

    -:% 5

    # 4

    6+7 : -%#/

    4 6;7

    4 53

    -%#/ 3

    < .0 :

    -%#/

    = >? = -:%

    6&87 :4

    @

    3

    8');3&8() A () A B&)88 C &(() - / "D

    $ / 8 ' ) ; 3 & 8 ( ) . ( ) 0 8 8 & ; 8 3 ;

    $ : 2 < GF& .+0 (';1 '+'1*

    5< GF& .+0 (';1 &1(&* 3< H

    -

    < / " / 2 E

    I / 188&

    E / &1+ .&(()0 F19)'

  • ! "" #

    "$ %

    %

    &

    ' ! #

    % &

    "" "$ (

    %

    %

    %

    )*+ %

    ( ,

    & % "- &

    ./0 1

    )

    , &

    % &

    & ,

    2#"3 3 "4$

    "5 &

    #2(*.

    %

    &

    (

    "" "$ +

    ,

    6

    %

    7"89 "0 &

    . : 7 6+ ./0

    1 &

    & 6 +

    * "- ' .

    & 7"89

    66

    ";

    (#" 2#"3 3

    "-5 0?+1+++16116

    +6+11++1166 "9 #6 0?66+1+6

    6+66+16++6++ $8 &

    ' @ "888 ' ( A

    6+ B "8 8"

    B)+ !

  • !" #$ % &

    '( ) $

    )

    ) ( &

    ' & $

    ' *

    ) $

    + $,- $

    $. /, #/,%

    &

    )0 '1 # 2

    3. /, )% ! " &

    $.

    4

    * 5 (

    ' #6 ! 7 /

    /8% ( 0

    (9(

    #)0) 3 (9(% #

    2 :(% $ 2

    )%

    ! )

    ; < #$ 2

    $ 2

  • !" #!" $%!& ! '

    ( #! ) *(

    + ( ,+ - #, %

    +& .& /0 1 2

    3( ! *.;/5 1 3( 9 2 ( (

    2 1< / =/1 < / " !< 5 ' & *!&

    >- =< / ( +( (

    6

  • ! " #$% &'

    ( # (

    )*

    # + ( !

    ,

    - -" .+

    # ( "

    *

    / 01 *

    "

    2 345 "/ *

    2

    *

    2 3+/65 "

    *

    .+# - -"( .+

    1!/

    ! "

    2

    34/65 !

    7 / /

    # /

    1( .+ **

    8

    # (/

    $% &'

    3 +5 " 7 )

    *

    7 " *

    - #( #$

    ) # 9!( #( *

    ) # 9( #( !

    ) # 98( #( "

    7 7

    # 9,(

    %! &

    . 8

    7 315

    7 ,! 3 / 15 "

    2

    :/ *

    *

    * ,!

    *

    8

    2 3 5 "

    * *

    :/

    # ( 3 5 "

    / *

    / ;/ *

    7

    7 *

    7*

    " ) *

    315 *

    *

    7 0 2

    > "

    )/ /

    *

    # (

    ! )

    3195/

  • !"#

    $

    !# %

    &

    '

    (

    )

    *

    + , +-.

    / ++ , +-.

    % .0 0%1

    !2#

    3 41'

    & 5* "46 !77# %

    41'

    2

    !8#

    516 !9# 2

    :

    5 6 4

    $ :

    5 6 ;

    $ 41'

    :

    7+

    5* 7 6 %

    & 5

    6 !77# ( 41'

    1 *

    1

    1

    /

    2 -. :

    "+

  • !

    "#$ % &% '(% ) % *+ ,#--./

    ( 0

    1 .-% #234#5-

    "6$ *% '(% ,#--3/ &

    7 8 52% 59493

    "3$ !% :; ,#--./ & <

    1

    2% #.#4#59

    "2$ % + =((% 7 ,#--3/ & >!

    < ? ( !

    ; #23% #.694#.32

    ".$ % % % 1% !1 ,#--3/ '@

    ( ( &! ? ! 6#%

    .9--4.ABB

    "5$ :% ; +% > ,#--3/ <

    ( %

    ? 8? #.%

    9A4AB

    "9$ :% ; '% > ,#--2/

    0 ( >!

    ,&!/ + 1 : 3% -3#4-35

    "A$ % % *% ;% *% % +C% ;% %

    8% ' ,#--3/ (! ?

    ( : 53% 63.4639

    "-$ D% &% +% :)% % E% % ; )

    % E ,#--6/ > ( +7

    +

    , /

    ( (

    ,&:/ ? #6% 6#5.46#5A

    "#B$ % * ,#-A-/ (

    &< ( ,E% +% E/%

    94#9 %

    ! F

    "##$ +% E% >% )% :(% 1% 1

    ,#--./ &

    0 ( (

    E 1 5#% 2B9242BA6

    "#6$ % ;% % E% ,#--./ =

    0 ( ( 0

    ( =E1 1 '

    #32% .#4.5

    "#3$ (% 1'% 1% * :% E ,#--3/

    ?

    0

    E

    1 .-% A234A.B

    "#2$ +% * ,#-A5/ 8 % 6 7 !

    % )(

    "#.$ F% % 7% ; 1(% ; ,#-A./ &

    1#3 ( ( <

    ? 1#3#A #- : 33% #B34

    ##-

    "#5$ % ;% =% E=% 1% ,#-A-/ 1

    (< ' 1% 6 (

    + ' % ( +% !F

    "#9$ % :% :% > % + ,#-9A/ <

    ( 1 : : #5B%

    6#.4662

    "#A$

    G

    (% ;% :% E *C(% ,#--#/ 8

    1

    < &

    =E1 1 E A5% A34-2

    "#-$ 8C% E% H% ;% >% ;% +H% ' % 1

    ,#--B/ >

    (

    E 1 .5%

    3B943#2

    "6B$ !% 8% ;% > :% E ,#--./ :

    (

    >!

    E 1 5#% 3A9.43AA3

    "6#$ % &% ;% >% % *% %

    > ,#--6/ 1

    0

    ( 0

    E

    1 .A% 5#24563

    "66$ % % *% % 1% 1 )% +;

    ,#--#/

    ( (

    ( ? 1 2% A294A.2

    "63$ !% :;% :% =% +% % 1%

    8% ) ,#--3/ & H

    ( 0 >! 1

    6% 3B.43#6

    "62$ % ;=% D% &% % 1 )% E ,#--3/

    ,/ (

    #3%

    ##.34##.2

    "6.$ % ) ,#-AB/ E

    I ( >! '

    ( 6.% -2A4-.#

    93