COMPARATIVE CHARACTERISTICS OF THE STRUCTURE AND BIODIVERSITY OF

SOIL MICROFLORA IN AREAS CONTAMINATED WITH RADIONUCLIDES AND SS NULES OF

UKRAINE EDUCATIONAL-RESEARCH FARM "VORZEL”

Illienko VladimirDepartment of Ecology and Sustainable Development

Supervisors: Doctor of Biological Sciences, Prof. Gudkov I.M. Candidate of Agricultural Science, Assistant Prof. Palamarchuk S.P.

NATIONAL UNIVERSITY OF LIFE AND ENVIRONMENTAL SCIENCES

Department of Radiobiology and Radioecology

Actuality of the topic

After the Chernobyl accident had accumulated much data on the effects of ionizing radiation on the fauna and flora, but least studied effects of the disaster for prokaryotes. Given the critical role of soil microorganisms in providing and maintaining soil fertility, the redistribution of nutrients and cleansing of pollutants such research is extremely important today.

Microbial complex of sod-podzolic soils that formed under different levels of radionuclide contamination of territories

Object of research

Subject of research

Effect of radioactive components on the soil microflora

Purpose: To study the biodiversity and structure of soil microbial complex formed in the territories

contaminated with radionuclides

Tasks:

1) on the investigated areas to measured gamma survey and to make soil sampling;

2) to determine the intensity of surface contamination by137Cs and 90Sr;

3) to assess the biodiversity of soil microorganisms in the conditions of radionuclide contamination of Chernobyl fallout;

4) to weed the dominant morphotypes in pure culture for further species identification;

5) to analyze the impact of radionuclide contamination on soil microflora composition.

Methods1.Sampling was carried out by standard methods:

soil – method of “envelope”, in accordance with State standard of Ukraine Б В.2.1-8-2001 (GOST 12071-2000) and State standard of Ukraine 4287:2004;

2. Before the measurements all samples were properly prepared.

3. Measurements:gamma-ray background – dosimeter-roentgenometer "Terra”;137Cs content in the samples - gamma-spectrometric method on RUB-01-P6.

4. Classical methods of microbiological testing

Description of places for sampling

#Code of place Location

1. Т1 Chystohalivka village, meadows, № 3 under power lines

2. Т2 Kopachi village rapper 180-5, meadows3. Т3 meadows between Zapollya and Zalesye4. Т4 meadows before village Dytiatki5. Т5 meadows before village Hocheva6. Т6 pine forest ERF "Vorzel"7. Т7 ERF "Vorzel" meadows use for grazing

Location of sampling sitesExclusion Zone ERF “Vorzel”

137Cs and 90Sr contamination of investigated soils

# Point of samplingSurface activity of soil,

kBq/m2

137Cs 90Sr1 ERF "Vorzel" meadows 26,2±1,5 3,9±0,022 ERF "Vorzel" forest 57,8±2 5,6±0,053 Hocheva 96,7±6 28,5±44 Dytiatki 117±7 50±85 Zalesye 287±45 95,6±56 Kopachi  2280±13 962±57 Chystohalivka 28000±25 15000±47

Structure and quantitative composition of functional groups of microorganisms

Biodiversity indexes of soil microorganisms communities

The total quantity of microorganisms for the investigated microcenosis, colony

units

Comparison of microcenosis ERF “Vorzel”

Conclusions:

Variation of surface soil contamination by 137Cs on soil sampling areas ranging from 26,2 ± 1,5 kBq/m2 (meadowsERF "Vorzel") to 28 000 ± 25 kBq/m2 (v. Chystohalivka), contamination by 90Sr from 3,9 ± 0,02 kBq/m2 to 15000 ± 47 kBq/m2 respectively

1.

2.Results of multysubstrat testing showed that the investigated areas are such functional groups of microorganisms: ammonificatjrs, oligonitrophiles, microorganisms that use organic and mineral forms of phosphorus

Analysis of microorganisms isolated from soil extracts showed that the biocoenosis, characterized by the lowest level of radionuclide contamination have the highest number of microorganisms. At the same time followed by the number of representatives of all microorganisms groups was the area of v. Kopachi and v. Chystohalivka, but its level of radionuclide contamination is the largest from analyzed soils

3.

4.For the territory of ERF "Vorzel": the number and diversity of microorganisms is higher for meadow biocenosis and it equaled 75 colony units, represented by 14 morphotypes. For forest biocenosis, which has twice higher specific activity of soil was found 29 colony units represented by 10 morphotypes

Thank you for attention!

I express my gratitude for the help: Director UIAR Prof. V.A. Kashparov,

postgraduate student of radiobiology and radioecology department O.Y. Parenyuk

The research results reported1. Паренюк О.Ю., Іллєнко В.В. Оцінка структури

ґрунтової мікрофлори радіонуклідно забруднених територій. – Львів, 2011 р.

2. Moscow Summer Ecology School (MOSES -2011): "Agro-Ecological Management and Engineering“. – 2011

3. Паренюк О.Ю., Іллєнко В.В. Аналіз функціональних груп грунтових мікроорганізмів з територій підданих радіонуклідному забрудненню. – Київ, НУБіП, 2011 р.