Hybrid Multi-Mode Microplate Reader
description
Transcript of Hybrid Multi-Mode Microplate Reader
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Hybrid Multi-Mode Microplate Reader
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Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3 Multi-Volume Multi-Sample Plate with 2 µL
microspots Specifications and Summary
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Synergy™ H4: Hybrid Technology
Filter-based optics: Use of dichroic mirrors increases
sensitivity about 5x compared to filter-only optics
Peak transmission for filters and mirrors is around 80%. Very efficient optical system
Because of high amount of energy delivered to samples, high sensitivity can be obtained with short read times
In summary, a fast and very sensitive optical system
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Synergy™ H4: Hybrid Technology
Monochromator-based optics: Use of two gratings per
monochromator provides smooth performance from 250 to 900 nm
Very large range of applications, including spectral scanning
Variable bandpass system adds flexibility
In summary, an extremely flexible optical system
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%CV Assay Window Z'
FilterMonochromator
Synergy™ H4: Hybrid Technology
Patent-pending design: Flexible monochromator system High-performance filter system Each with its strengths and
weaknesses Extremely versatile instrument
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Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
![Page 7: Hybrid Multi-Mode Microplate Reader](https://reader035.fdocument.pub/reader035/viewer/2022062410/56815c7e550346895dca9321/html5/thumbnails/7.jpg)
Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
![Page 8: Hybrid Multi-Mode Microplate Reader](https://reader035.fdocument.pub/reader035/viewer/2022062410/56815c7e550346895dca9321/html5/thumbnails/8.jpg)
Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
![Page 9: Hybrid Multi-Mode Microplate Reader](https://reader035.fdocument.pub/reader035/viewer/2022062410/56815c7e550346895dca9321/html5/thumbnails/9.jpg)
Filter wheels: Easy front access No tool required Easy load/unload software function
Dichroic mirrors: Easy side access Easy load/unload
software function
High-performance, easy-to-use filter system
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Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
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300 µl200 µl 100 µl
300 µl200 µl 100 µl
Top fiber
Automated Z-Focusing
If no Z-focusing available, performance issues in low volumes, or use of adapters required.
With Z-focusing, reading height can be precisely adjusted (100 µm resolution) for best performance on all plate types, and with all volumes.
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Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
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Time (minutes)F
luor
esce
nce
(RF
U)
0:00:00 0:01:00 0:02:00 0:03:00 0:04:00 0:05:00 0:06:00 0:07:00 0:08:005000
10000
15000
20000
25000
30000
35000
Baseline Response
Trigger Reagent Injection
Dual Reagent Dispenser Option
Two reagent dispensers Volume range 5 µL - 1000 µL Enables dispense/read processes Ion channel assays, flash
luminescent assays, time-sensitive kinetic assays
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Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
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Advanced 4-ZoneTM Temperature Control System
4 independent heaters with 4 monitoring sensors
Precision: +/- 0.5°C at 37°C Range: RT to 65°C Enables applications with strict
temperature uniformity requirements (e.g. ORAC assays)
Enables high-temperature assays (e.g. Invader assays at 62°C)
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Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
![Page 17: Hybrid Multi-Mode Microplate Reader](https://reader035.fdocument.pub/reader035/viewer/2022062410/56815c7e550346895dca9321/html5/thumbnails/17.jpg)
Sixteen 2 µL microspots Enables low volume A260 nucleic
acid quantification dsDNA detection limit 2 ng/ µL Can also be used for A280
protein quantification, as well as fluorescence or luminescence detection
Take3™ Multi-Volume Multi-Sample Plate with 2 µL microspots
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Contents
Synergy H4 – Hybrid Technology Key Features and Benefits
Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots
Specifications and Summary
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Summary: Versatility Redefined
Hybrid Technology: Monochromators and Filters Quadruple Grating Optical Architecture Variable Bandpass Selection Dichroic Mirrors and Filters for High Performance Dual Reagent Dispenser Option Temperature Control System to 65°C Take 3™ Multi-Volume Plate with 2 µL microspots
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Visit www.biotek.com for more information