Glucose Biosensors Final
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Transcript of Glucose Biosensors Final
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B Y
H A R S H A V A R D H A N . G . B .
S A N G A R . S I
A R V I N D K U M A R . M
K A R T H I K E Y A N . R
J E Y A S U R I Y A K A N T H . R
K I S H O R E . T
Glucose biosensors1
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WORKING OF A BIOSENSOR2
BIORECEPTOR NUCLEIC ACIDS,TISSUES.,ETC
TRANSDUCER OPTICAL TRANSDUCER,PIEZOELECTRIC
CRYSTALS
AMPLIFIER
DIGITALDISPLAY
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BIO-RECEPTOR
Includes a species of biological origin such as enzyme,antibody or DNA strand
The bio-receptor must be capable of recognizing theanalyte of interest with a high degree.
i.e. binding between a receptor and analyte takesplace.
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TRANSDUCER
Specifications:
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TRANSDUCER5
Translates the binding between receptor and analyteinto measureable physical change.
Possible changes are production of electrons,protons or an electrochemical species.
Eg: hydrogen peroxide, change in conductivity.
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SCHEMATIC CONFIGURATION6
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CROSS-SECTIONAL VIEW7
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WORKING
Glucose reacts with glucoseoxidase(GOD) to form gluconicacid. Two electrons & two protonsare also produced.
Glucose mediator reacts withsurrounding oxygen to form H2O2and GOD.
Now this GOD can reacts withmore glucose.
Higher the glucose content, higherthe oxygen consumption.
Glucose content can be detected byPt-electrode.
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POLYMERS IN BIOSENSORS9
For determination of glucose, the glucose oxidase isimmobilized in polyaniline.
Aniline is electrochemically polymerized from asolution containing glucose oxidase into platinumelectrodes
RXN
INVOLVED:
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APPLICATIONS
The foremost application of a glucose biosensor is to check the
sugar level in a persons body.
In food industry, these biosensors are used to monitor the
freshness of food.
Keeping glucose levels in check is crucial to minimize theonset of the damage caused by diabetes
Environmental applications e.g. the detection of pesticides and
river water contaminants.
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Determination of drug residues in food, such asantibiotics and growth promoters.
Drug discovery and evaluation of biological activity ofnew compounds.
Analytical measurement of folic acid, biotin, vitamin
B12 and pantothenic acid.
Using bacteria researcher can detect pollutants insamples.
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DRAWBACKS12
The monitoring systems currently in general usehave the drawback of below optimal number ofreadings, due to their reliance on a drop of fresh
blood.
Some continuous glucose monitors arecommercially available, but suffer from the severedrawback of a short working life of the probe.
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