Microbiological Test (Stamp Method) for Food HygieneMicrobiological Test (Stamp Method) for Food Hygiene

Simple and easy prepared media for detection of contaminant from the specimen directly!

Most kinds of media for your test purpose Most kinds of media for your test purpose and targeted microorganisms are availableand targeted microorganisms are available

Food Stamp

What is What is ““Food StampFood Stamp””??

For keeping quality and safety of the products, and also prevent food borne accident.

Best solution for your routine test!You can start food hygiene test anytime, even you

have never experienced microbiology!

It is simple prepared culture media that can be handled by anyone.

Intended Use, Food StampIntended Use, Food Stamp

It is useful for “Self Hygiene Control” at all kinds of food related industries.

① Test for Food Manufacturing Environment:Hygiene control for manufacturing apparatus, Kitchen apparatus (food processor, cutting board, etc.) and manufacturing environment (wall, production line, working surface, etc.).

② Test for confirmation of cleanliness, disinfection: Confirm cleanliness and disinfection.

③ Hygiene education for employeesHygiene education and enlightenment

You can select the medium by purpose and use! You can select the medium by purpose and use! There are 10 kinds of media are prepared.There are 10 kinds of media are prepared.

① Standard method(Total viable count)

② Desoxycholate(Coliform)

③X-GAL(Coliform)

④ XM-G(Coliform/E. coli)

⑤ TCBS(V. parahaemolyticus)

⑥ TGSE(S. aureus)

⑦ MLCB(Salmonella)

⑧ Cereus(B. cereus)

⑨Sabouraud(Yeast and Mold)

⑩ Potato Dextrose with CP(Food poisoning Yeast and Mold)

Various kinds of Food StampVarious kinds of Food Stamp

Potato Dextrose agar with CPHygiene control for Meats and manufacturing environment.

Sabouraud agarYeast and Mold

Hygiene control for Grains / Meats / Vegetables and manufacturing environment.

Cereus agarB. cereus

Hygiene control for Egg / Meat and manufacturing environment.

MLCB agarSalmonella

Hygiene control for Meat / Environment andEmployees.

TGSE agarS. aureus

Test for sea food and hygiene control for its processing environment.

TCBS agarV. parahaemolyticus

XM-G agarColiform / E. coli

X-GAL agarHygiene control for food and manufacturing environment.Control for fecal pollution by coliform isolation.

Desoxycholate agarColiform

Hygiene control for food and manufacturing environment.

Standard method agarTotal viable count

Purpose of testName of mediumTarget bacteria

Food Stamp and test purposesFood Stamp and test purposes

Operation of Food StampOperation of Food Stamp

Cut off a side of bag, and take out Food Stamp from the bag. Hold the cap of Food Stamp and cut off each plate by bending.

Take off the cap of Food Stamp plate and gently press the medium against the surface of specimen immediately. The surface of medium / agar is elastic enough to be pressed firmly against the specimen.Caution:1. Wash or clean up the place of specimen where Food Stamp used.2. Press against the different parts of the specimen when several kinds of Food

Stamps use simultaneously.

Put the cap again immediately after pressing.Write necessity information (date/name of Specimen etc.) on the cap with oil felt pen.Caution:Do not write any information inside of thecap. It may hindrance to read surface ofthe medium.

Put the Food Stamp plate into the incubator.In case of the incubator was not available,It may be possible to keep plates at room Temperature for 1.5 – 2 times longer time.Incubation Temp.: Incubation Time:Fungi 20-25 ℃ 2 – 3 days(Sabouraud and PDA) Others 35-37 ℃ 1 – 2 days XM-G 35℃ 20±2hours.

All colonies grown on the medium surfaceshall be counted. A piece of Food Stamphas a medium surface of 10 cm2. And it maybe recommended to use the square imprintedon the back of container. (1 cm2 / square)

Blue colonies (E. coli) / Red colonies (Coliforms)

④ XM-G agarBlue Green-Blue colonies (Coliforms)③ X-GAL agarRed colonies (Coliforms)② Desoxycholate agarCount all colonies grown on the surface.① Standard Method agar

InterpretationName of medium

① Standard Method agar(Total viable count)

② Desoxycholate(Coliform)

③X-GAL(Coliform)

④ XM-G(Coliform/E. coli)

Mainly used for controlling of fecal pollution by coliform isolation.

Interpretation of Food Stamp (1)Interpretation of Food Stamp (1)

InterpretationName of medium

White, slightly thick colonies with an irregular rim⑧ Cereus agarBlack and/or black center colonies (Salmonella) Note2⑦ MLCB agarBlack colonies with an opaque ring around these colonies (S. aureus)⑥ TGSE agarGreen colonies (V. parahaemolyticus) Note1⑤ TCBS agar

Note1: V. alginolyticus is not pathogenic, their existence, if too many yellow colonies present, implies a risk of contamination by V. parahaemolyticus. Note2: Citrobacter or other organisms may also develop black colonies just like Salmonella. It is recommended that certain identification tests should be performed simultaneously.

⑤ TCBS(V. parahaemolyticus)

⑥ TGSE(S. aureus)

⑦ MLCB(Salmonella)

⑧ Cereus(B. cereus)

Food poisoning bacteria (basically it should not be presented in the products)

Interpretation of Food Stamp (2)Interpretation of Food Stamp (2)

InterpretationName of medium

Count all fungi grown on the surface (bacteria inhibit growth)

⑩ Potato Dextrose agar with CP

Count all fungi grown on the surface⑨ Sabouraud agar

⑨Sabouraud(Fungi)

⑩ Potato Dextrose with CP(Food poisoning Yeast and

Mold)

Mold Yeast

Use these plates to detect fungi as contaminant

Interpretation of Food Stamp (3)Interpretation of Food Stamp (3)

Either one of two egg yolk reactions is positive, Identify those colonies are Staphylococcus aureus.

Lecithinase ReactionLecithinase Reaction

It is observed “Turbid” inside of medium around colonies from top surface.

It is observed “Pearly luster” on the surface of medium around colonies from sidelong glance.

Egg Yolk Reaction of Egg Yolk Reaction of Staphylococcus aureusStaphylococcus aureus

Lipase ReactionLipase Reaction

×＋＋＋Severe contaminationOver 100

◎－ or ±Negative / less contamination0 ～ 9

〇＋Slight contamination 10 ～ 29

△＋＋Moderate contamination30 ～ 99

CleanlinessMarkJudgmentColonies/plate

Judgment of Cleanliness by the number of colonies isolatedJudgment of Cleanliness by the number of colonies isolated

● Quantitative test: Judged by the number of colonies isolated(Contaminants)

① Standard method(Total viable count)

② Sabouraud(Yeast and Mold)

③ Potato Dextrose with CP(Food poisoning

Yeast and Mold)

● Qualitative test: Judged by (Food poisoning bacteria)

① Desoxycholate(Coliform)

② X-GAL(Coliform)

③ XM-G(Coliform/E. coli)

④ TCBS(V. parahaemolyticus)

⑤ MLCB(Salmonella)

）⑥ TGSE

(S. aureus)

⑦ Cereus(B. cereus)

Example of use, Food Stamp (1)Example of use, Food Stamp (1)Hygiene check (kitchen apparatus)

Total Viable Count

Coliforms

S. aureus

Cutting board

Kitchen Knife

Cooking table

Dishcloth Sponge

Check for cleaning, sterilization

Total Viable Count

Coliforms

S. aureus

Before washing

After washing by water

After washing by Detergent

After spraying of

Ethanol

Cooking table

Example of use, Food Stamp (2)Example of use, Food Stamp (2)

Hand Washing

Total Viable Count

Before

Coliforms

S. aureus

After AfterBefore

Ms. TS Mr. MR

Example of use, Food Stamp (3)Example of use, Food Stamp (3)Check for Hand washing (for employees education)

Hygiene control for food / material (Sea Food)

Example of use, Food Stamp (4) Example of use, Food Stamp (4) -- 11

Total Viable Count

Coliforms

Tuna

Vibrio parahaemolyticus

Scallop Ark shell Prawn Garnish (Radish)

Food Stamp application for Sea Food

Example of use, Food Stamp (4) Example of use, Food Stamp (4) -- 11Hygiene control for food / material (Daily dishes)

Total Viable Count

Coliforms

S. aureus

Sandwich(Lettuce)

Rice Ball(Rice)

Sandwich(Ham)

Tofu(soybean

curd )

Salad

Food Stamp application for Daily Dishes

Total Viable Count

Coliforms

S. aureus

Top shelf

Refrigerator

Second shelf

Internal Surface

Doorknob

Direction for Use (1)Direction for Use (1)Isolation data will be changed where the places stamped.

Cooking table

Total Viable Count

Coliforms

S. aureus

Before work During work After washing

Direction for Use (2)Direction for Use (2)Isolation data will be changed when Food Stamp used.

Adaptable test objects, Non adaptable Adaptable test objects, Non adaptable objects for Food Stampobjects for Food Stamp

1) Adaptable objects:

・ Kitchen apparatus (cutting board / kitchen knife / dish cloth, etc.)

・ Cooking table, Work bench, Wall, Floor

・ Instruments (Slicer / Refrigerator, etc.)

・ Employee’s Hands

・ Other (include foods that can be stamped)

2) Non adaptable objects:

・ Liquid surface・ Not smooth and irregular surfaces

・ Acquire lots of oil on the surface(thin film of oil will not be the problem)

Advantages and application of Food StampAdvantages and application of Food StampEasy and simple stamp media that

anyone can be used for food microbiology test!Simple Operation is one of the major advantages!

Applicable for “Self Hygiene Control”.1) Test for Manufacturing Environment

Hygiene control for the manufacturing apparatus / kitchen apparatus and manufacturing environment.

2) Confirmation of Cleanliness and SterilizationConfirmation of washing and effect of disinfection (before and after)

3) For the employees hygiene educationEducation and hygiene control for your Employees