Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and
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Transcript of Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and
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Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) andKaryotyping of parental, vector-infected and TG2-transfected LNCaP cells (B)ABLNCaP LNCaP-EV LNCaP-TG2Relative cell growth over 3 daysHistonesp65TG2IBLNCaPCont. LNCaPEVLNCaPTG2 C N C N C N C NPC3
Fig. S2. Co-localization of TG2 and NF-B in nuclear fraction of prostate cancer cells (A). Transient down regulation of NF-kB partially restored AR expression in PC3 cells (B). Putative NF-B binding sites in the promoter of AR gene (C and D).Exon 1Cont siRAp65Actinp65 siRAR
B-38 bpGACCCGACTCGCAAACTGTTGCATTTGCTCTCCACCTCCCAGCGCCCCCTCCGAGATCCCGGGGAGCCAGCTTGCTGGGAGAGCGGGACGGTCCGGAGCAAGCCCAGAGGCAGAGGAGGCGACAGAGGGAAAAAGGGCCGAGCTAGCCGCTCCAGTGCTGTACAGGAGCCGAAGGGACGCACCACGCCAGCCCCAGCCCGGCTCCAGCGACAGCCAACGCCTCTTGCAGCGCGGCGGCTTCGA AGCCGCCGCCCGGAGCTGCCCTTTCCTCTTCGGTGAAGTTTTTAAAAGCTGCTAAAGACTCGG +246 bp
-760 bpGGGTGATTTTGCCTTTGAGAGTCTGGATGAGAAATGCATGGTTAAAGGCAATTCCAGACAGGAAGAAAGGCAGAGAAGAGGGTAGAAATGACCTCTGATTCTTGGGGCTGAGGGTTCCTAGAGCAAATGGCACAATGCCACGAGGCCCGATCTATCCCTATGACGGAATCTAAGGTTTCAGCAAGTATCTGCTGGCTTGGTCATG -460 bp
CDFig. S3. TG2 and AR protein expression in selected prostate tumor samples (A). TG2 expression Is associated with increased accumulation of HIF-1 protein in prostate cancer cells (B)
TG2 AR
LnCap-EVLnCap-TG2PC3 HIF-1 TG2 -actin ABGene designationPrimer Sequence (5-3)
AR F: TTGGATGGCTCCAAATCACR: GCAATGATACGATCG AGTTCC
E-cadherinF: TCCCATCAGCTGCCCAGA A A R:TGACTCCTGTGTTCCTGT TA
N-cadherinF: ACAGTGGCCACCTACAAAGGR: CCGAGATGGGGTTGATAATG
Vimentin F: GAG AACTTTGCCGTTGAA GCR: GCTTCCTGTAGGTGGCAATC
FibronectinF: CAGTGGGAGACCTCGAGAAG R:TCCCTCGGAACATCAGAAAC
ZEB1 F: CTGAAGAGGACCAGAGGCAG R: CCCAGAACTGCGTCACATGTC
ZEB2 F: AAGTACCGCCACGAGAAGAA R: TTGTTTCTCATTCGGCCATT
SnailF: TTCTTCTGCGCTACTGCTGCGR: GGGCAGGTATGGAGAGGAAGA TG2 F: TATGGCCAGTGCTGGGTCTTCGCC R: GGCTCCAGGGTTAGGTTGAGCAGGGAPDH F: ACCACAGTCCATGCCATCAC R: TCCACCACCCTGTTGCTGTATable S1 Primers used to amplify various transcripts